FastQCFastQC Report
Thu 26 May 2016
SRR937788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484084
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.6564976326422687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26220.5416415332876112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.39414647044727774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.21359929268474068No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6840.14129779129242032No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.12931639963312153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.10535361631452392No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCG554.9577313E-543.1837439
GGTATCA25500.028.7083131
GTATCAA35250.027.1059151
ATAGCCC2002.0469088E-823.7510593
GTACATG26350.023.2721351
AGTACTT23800.023.25208712-13
TACATGG25900.022.5589222
TAGCCCT3600.022.4315554
ATCAACG42900.022.2562373
GAGTACT23350.022.17443712-13
TCAACGC43300.022.0506364
GTCCTAG1954.179983E-721.9399191
ATGGGCG652.9422122E-421.92179118-19
CAACGCA43200.021.8817635
GTATACT1754.039677E-621.7152544
AACGCAG43750.021.6044466
TATCAAC44150.021.5185152
GTAGACC3800.021.2509463
TCTATAC2551.1868906E-820.491113
GTACTTT25400.020.38266214-15
Produced by FastQC (version 0.11.5)