##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937788_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 484084 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.254707860619234 33.0 31.0 34.0 31.0 34.0 2 32.46869964716867 34.0 31.0 34.0 31.0 34.0 3 32.56941150709381 34.0 31.0 34.0 31.0 34.0 4 36.17283157468538 37.0 35.0 37.0 35.0 37.0 5 36.034549788879616 37.0 35.0 37.0 35.0 37.0 6 36.12169168987201 37.0 35.0 37.0 35.0 37.0 7 36.085954090612375 37.0 35.0 37.0 35.0 37.0 8 36.08711298039183 37.0 35.0 37.0 35.0 37.0 9 37.74735376504904 39.0 38.0 39.0 35.0 39.0 10-11 37.706393931631695 39.0 37.0 39.0 35.0 39.0 12-13 37.64506986390792 39.0 37.0 39.0 35.0 39.0 14-15 38.88630382330339 40.0 38.0 41.0 35.0 41.0 16-17 38.866145131836625 40.0 38.0 41.0 35.0 41.0 18-19 38.874407127688585 40.0 38.0 41.0 35.0 41.0 20-21 38.82661996678262 40.0 38.0 41.0 35.0 41.0 22-23 38.68074652333066 40.0 38.0 41.0 34.5 41.0 24-25 38.62519521405376 40.0 38.0 41.0 34.0 41.0 26-27 38.53641620049413 40.0 38.0 41.0 34.0 41.0 28-29 38.41728501665 40.0 38.0 41.0 34.0 41.0 30-31 38.43871621454128 40.0 38.0 41.0 34.0 41.0 32-33 38.33076387569099 40.0 38.0 41.0 34.0 41.0 34-35 38.192180489336565 40.0 38.0 41.0 34.0 41.0 36-37 38.03025817833269 40.0 37.0 41.0 33.5 41.0 38-39 37.879707860619234 40.0 37.0 41.0 33.0 41.0 40-41 37.68015158526206 40.0 37.0 41.0 33.0 41.0 42-43 37.44868349294751 40.0 37.0 41.0 33.0 41.0 44-45 37.11146825757513 40.0 37.0 41.0 32.5 41.0 46-47 36.72392911147652 40.0 36.0 41.0 31.0 41.0 48-49 36.38589790201701 40.0 36.0 41.0 30.5 41.0 50-51 36.12431933300832 40.0 36.0 41.0 30.0 41.0 52-53 35.9058748068517 39.0 35.5 41.0 29.0 41.0 54-55 35.711590343824625 39.0 35.0 41.0 28.5 41.0 56-57 35.438435271564444 39.0 35.0 40.0 27.5 41.0 58-59 35.21709868535213 39.0 35.0 40.0 27.0 41.0 60-61 34.912418092727705 38.0 35.0 40.0 26.0 41.0 62-63 34.56963357599095 38.0 34.0 40.0 26.0 41.0 64-65 34.21151494368746 38.0 34.0 40.0 24.5 41.0 66-67 33.8163314631345 37.0 34.0 40.0 23.0 41.0 68-69 33.3688378463242 37.0 33.0 39.0 22.0 41.0 70-71 32.98528664446667 36.0 33.0 39.0 20.5 40.0 72-73 32.54479491162691 36.0 32.0 39.0 19.5 40.0 74-75 32.06636554812801 35.0 32.0 38.0 18.0 40.0 76-77 30.76399963642674 34.0 30.5 36.0 14.0 39.0 78-79 31.27017418464564 35.0 31.0 37.0 13.0 39.0 80-81 31.04226745771395 35.0 31.0 36.5 9.0 39.0 82-83 30.682245230166664 35.0 31.0 36.0 7.0 37.5 84-85 30.253567562654414 34.0 31.0 35.5 2.0 37.0 86-87 29.8160102378926 34.0 30.5 35.0 2.0 36.5 88-89 29.39279236661406 34.0 30.0 35.0 2.0 36.0 90-91 28.916975153072606 34.0 29.0 35.0 2.0 36.0 92-93 28.508094669520165 34.0 29.0 35.0 2.0 35.0 94-95 28.090323167053654 34.0 29.0 35.0 2.0 35.0 96-97 27.61731744903777 34.0 28.0 35.0 2.0 35.0 98-99 26.93094793465597 33.0 26.5 35.0 2.0 35.0 100-101 24.7058702621859 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07810706770428766 1101 2 0.07229678001623796 1101 3 0.02586283498474984 1101 4 0.04373037456216622 1101 5 -0.0053674118901483325 1101 6 0.012997157154735817 1101 7 0.028130302570836818 1101 8 0.04643570981826173 1101 9 0.15378941448992833 1101 10-11 0.12217019167153609 1101 12-13 0.21880217892252318 1101 14-15 0.17815527400038178 1101 16-17 -0.04130366681343389 1101 18-19 0.20903426214395182 1101 20-21 0.15976406983321567 1101 22-23 0.2553850350068174 1101 24-25 0.11141713943584364 1101 26-27 0.0693902110350777 1101 28-29 0.03755227353182278 1101 30-31 0.08367373773437947 1101 32-33 0.1491920414996386 1101 34-35 0.14316153131092335 1101 36-37 0.29943125813910854 1101 38-39 0.4977155364301069 1101 40-41 0.43646916464027896 1101 42-43 0.4078103770128223 1101 44-45 0.6402691194050121 1101 46-47 0.5362018996851674 1101 48-49 0.6431161497409903 1101 50-51 0.6936083973082461 1101 52-53 0.5388168886668439 1101 54-55 0.6592504314025831 1101 56-57 0.7815668220128771 1101 58-59 0.9156699839131051 1101 60-61 0.9273004222457644 1101 62-63 0.9729829453525625 1101 64-65 0.9105431910012669 1101 66-67 0.9051689769337656 1101 68-69 0.9689865183872968 1101 70-71 0.994333192017109 1101 72-73 1.2346587600912535 1101 74-75 1.06545628823633 1101 76-77 0.9708642376177892 1101 78-79 0.9937829908554683 1101 80-81 1.1952042104129141 1101 82-83 1.2016147433129944 1101 84-85 1.0726420399263894 1101 86-87 1.354307428135801 1101 88-89 1.3157764071529883 1101 90-91 1.361231728531628 1101 92-93 1.4485291116005072 1101 94-95 1.2581861157409477 1101 96-97 1.306311707579372 1101 98-99 1.7281901071093735 1101 100-101 2.149091433237423 1102 1 0.23702347629500053 1102 2 0.0998804583607864 1102 3 0.10484793503590595 1102 4 0.15487018411093345 1102 5 -0.01978354707574681 1102 6 0.06661983793599546 1102 7 0.08726518326793098 1102 8 0.14483257309302644 1102 9 0.1153274883571811 1102 10-11 0.11357934935698921 1102 12-13 0.24587621501199663 1102 14-15 0.2895621890079312 1102 16-17 0.2931016581606798 1102 18-19 0.29833127081479205 1102 20-21 0.2558174606866288 1102 22-23 0.21770426634785878 1102 24-25 0.04476991771059602 1102 26-27 0.016274022680370592 1102 28-29 0.09562755191366534 1102 30-31 0.3354716598555001 1102 32-33 0.1978209841400016 1102 34-35 0.2468173482882463 1102 36-37 0.28414577028105725 1102 38-39 0.2614191804037205 1102 40-41 0.31501555657469993 1102 42-43 0.5383669895108412 1102 44-45 0.5735752802176179 1102 46-47 0.8458237438719749 1102 48-49 0.6967463901107038 1102 50-51 0.8580779535855996 1102 52-53 0.737949047912096 1102 54-55 0.752192421640629 1102 56-57 0.8838927813876509 1102 58-59 0.862040373509096 1102 60-61 1.0344903468262672 1102 62-63 1.2283748218188109 1102 64-65 1.161225443859422 1102 66-67 1.323963247773655 1102 68-69 1.3152786662427687 1102 70-71 1.2255948621317856 1102 72-73 1.1187425505697028 1102 74-75 1.1239555335374227 1102 76-77 0.8846490211741767 1102 78-79 1.079804177862048 1102 80-81 1.1729928795977642 1102 82-83 1.1543868443998129 1102 84-85 1.0971930633686746 1102 86-87 1.1408076410642103 1102 88-89 1.3213585332620426 1102 90-91 1.466079440622181 1102 92-93 1.3906900703579126 1102 94-95 1.245737363451024 1102 96-97 1.6201448725018714 1102 98-99 1.5936036039986021 1102 100-101 2.2696479701395553 1103 1 0.10792092670484266 1103 2 0.13246780296496752 1103 3 0.043180680190083365 1103 4 0.03188691725294035 1103 5 -0.12555591508501607 1103 6 -0.08369045431135902 1103 7 0.017887232661664143 1103 8 0.036641457039735315 1103 9 0.15962403718825868 1103 10-11 0.14998209004988183 1103 12-13 0.0617442342687653 1103 14-15 0.09178750379475531 1103 16-17 -0.08539994989892818 1103 18-19 0.0010400022788346064 1103 20-21 -0.061579546924193096 1103 22-23 0.06870073061431725 1103 24-25 -0.11107952396312015 1103 26-27 0.022193546455014257 1103 28-29 0.08931683351386965 1103 30-31 -2.890222547549115E-4 1103 32-33 0.12457353606068011 1103 34-35 0.4076757458218836 1103 36-37 0.3248366699288141 1103 38-39 0.32165815870324366 1103 40-41 0.25374449032797486 1103 42-43 0.35306059256315336 1103 44-45 0.5614249416261856 1103 46-47 0.6452674151752689 1103 48-49 0.9207818872774567 1103 50-51 1.0462384066389134 1103 52-53 1.1662647136754103 1103 54-55 1.0773117776177514 1103 56-57 1.3403544842661788 1103 58-59 1.1892771744359791 1103 60-61 1.1269354630526962 1103 62-63 1.2383652666820097 1103 64-65 1.3106326591529722 1103 66-67 1.3498020689294634 1103 68-69 1.099775373549285 1103 70-71 1.203713765321936 1103 72-73 1.1739326212177836 1103 74-75 1.113426571817893 1103 76-77 0.8963856922577342 1103 78-79 1.1260600291348446 1103 80-81 1.2411223367195028 1103 82-83 1.127336984564014 1103 84-85 1.1303563796337137 1103 86-87 1.350775945976281 1103 88-89 1.3693539521232836 1103 90-91 1.1515839318694105 1103 92-93 1.4090287749876573 1103 94-95 1.5112569697927007 1103 96-97 1.621130350025659 1103 98-99 1.6873654646867067 1103 100-101 2.2821553778675643 1104 1 0.07448853127274901 1104 2 0.1009635046052324 1104 3 0.08077963599903626 1104 4 0.0980438180122789 1104 5 -0.14928830286423533 1104 6 0.009013388277686829 1104 7 -0.025192025520524908 1104 8 0.10870381939443519 1104 9 0.008824312691437797 1104 10-11 0.027374673572417407 1104 12-13 0.08244417389615677 1104 14-15 2.9640474934922167E-5 1104 16-17 0.08214892215364955 1104 18-19 0.11891114502179079 1104 20-21 -0.06384989717035694 1104 22-23 0.16563278166743345 1104 24-25 -0.002439650830631024 1104 26-27 -0.2272091048378897 1104 28-29 -0.3118400247295412 1104 30-31 -0.1651179381837693 1104 32-33 -0.20491529500655048 1104 34-35 -0.06309987214704194 1104 36-37 -0.11480347530557111 1104 38-39 -0.08775529163391127 1104 40-41 -0.119292336963845 1104 42-43 0.12494991511445619 1104 44-45 0.16037862478052745 1104 46-47 0.2131079464542296 1104 48-49 0.274551561520461 1104 50-51 0.6083169675599791 1104 52-53 0.6461196068328974 1104 54-55 0.5309162775029677 1104 56-57 0.49812150551563406 1104 58-59 0.47918261902497505 1104 60-61 0.3712106941267237 1104 62-63 0.47814125028786947 1104 64-65 0.4825808662565265 1104 66-67 0.6591479189502962 1104 68-69 0.3220826276159343 1104 70-71 0.4286814532210599 1104 72-73 0.5872979265246236 1104 74-75 0.6124465798108893 1104 76-77 0.32826349299411106 1104 78-79 0.4850793568730829 1104 80-81 0.26643419100893695 1104 82-83 0.5562514255221558 1104 84-85 0.6708783050947993 1104 86-87 0.6939921054279985 1104 88-89 0.7778171715989792 1104 90-91 0.6953051804770141 1104 92-93 0.8737586288179173 1104 94-95 1.127274418327179 1104 96-97 1.5138031559914928 1104 98-99 1.7114996279308414 1104 100-101 2.1635221372189406 1105 1 0.01046079129472588 1105 2 0.024275367679408077 1105 3 0.012790482007886794 1105 4 0.044358090190648625 1105 5 -0.128614358485315 1105 6 0.020151715210261045 1105 7 0.0022959541583276177 1105 8 -0.17244569626474515 1105 9 -0.01697023809631304 1105 10-11 -0.043865259093628595 1105 12-13 -0.03915510841255099 1105 14-15 0.041896645827385726 1105 16-17 -0.30125144273995375 1105 18-19 -0.18904595477963682 1105 20-21 0.07957748194603198 1105 22-23 -0.07087774682349846 1105 24-25 -0.11119205741483995 1105 26-27 -0.15760716142664677 1105 28-29 -0.2985042722120781 1105 30-31 -0.2011889815577277 1105 32-33 -0.44525115840328766 1105 34-35 -0.4838867117853738 1105 36-37 -0.22970968152085902 1105 38-39 -0.22290200507146807 1105 40-41 -0.23083688523161072 1105 42-43 -0.17241821415062475 1105 44-45 0.09269690877258796 1105 46-47 -0.2062223427390748 1105 48-49 -0.3085474133502686 1105 50-51 -0.13999212353238732 1105 52-53 -3.418323800588041E-4 1105 54-55 -0.19539839787276492 1105 56-57 -0.29738183598244206 1105 58-59 -0.014260383988919045 1105 60-61 -0.13264992054764235 1105 62-63 -0.07788302219289989 1105 64-65 0.13051901578226932 1105 66-67 -0.0823572167760176 1105 68-69 -0.37161338173824987 1105 70-71 -0.21438808824274957 1105 72-73 -0.32210866776811997 1105 74-75 0.1230859500166197 1105 76-77 0.018718803607271184 1105 78-79 0.33337131207785475 1105 80-81 0.01570087581948343 1105 82-83 0.1653717496928273 1105 84-85 0.17784857936980103 1105 86-87 0.0894732710715509 1105 88-89 0.20952561959155958 1105 90-91 -0.10797986997305742 1105 92-93 0.6184288637811761 1105 94-95 0.6875121031679683 1105 96-97 0.4980834991124432 1105 98-99 0.28201380535783827 1105 100-101 0.5262435177666838 1106 1 -0.2794529730580315 1106 2 -0.17760815861213786 1106 3 -0.15754477296694347 1106 4 -0.07354493837986809 1106 5 -0.19368018661751307 1106 6 -0.13142768352240353 1106 7 -0.15524688574193846 1106 8 -0.10327454312103157 1106 9 -0.08120229615847308 1106 10-11 -0.05732469022421327 1106 12-13 -0.28441850034801774 1106 14-15 -0.31939974752151556 1106 16-17 -0.21508726150216262 1106 18-19 -0.029394867702279726 1106 20-21 -0.4914557576874188 1106 22-23 -0.0018852586947915029 1106 24-25 -0.202509731752194 1106 26-27 -0.30188908687966176 1106 28-29 -0.3487885481026254 1106 30-31 -0.32812451922030306 1106 32-33 -0.6033061713465742 1106 34-35 -0.832613028745051 1106 36-37 -0.6059940844620826 1106 38-39 -0.2856928320125647 1106 40-41 -0.5817826187110526 1106 42-43 -0.38346527054523705 1106 44-45 -0.7461591586913201 1106 46-47 -0.5113782180388355 1106 48-49 -0.525085441206123 1106 50-51 -0.24521390637101348 1106 52-53 -0.304993475304137 1106 54-55 -0.8208132977409548 1106 56-57 -0.8164870815872831 1106 58-59 -0.6026122190771375 1106 60-61 -0.5480976947785834 1106 62-63 -0.13101799765623667 1106 64-65 -0.8229883769790192 1106 66-67 -0.6405821600002426 1106 68-69 -0.7211020027619597 1106 70-71 -0.2898204575790686 1106 72-73 -0.4167892147212875 1106 74-75 -0.42339871655475747 1106 76-77 -0.18633304895041647 1106 78-79 0.24446363027627882 1106 80-81 -0.16026828262859283 1106 82-83 -0.2678591490319029 1106 84-85 -0.05578967558914627 1106 86-87 -0.01851186399920124 1106 88-89 -0.10093462148709875 1106 90-91 0.029342060561987182 1106 92-93 0.202008357801855 1106 94-95 0.28350286023119153 1106 96-97 0.7458640825926786 1106 98-99 1.083993413724123 1106 100-101 1.4199060711676736 1107 1 -0.010538104952516392 1107 2 -0.06501823055458544 1107 3 -0.22388170342257752 1107 4 -0.024654051089704865 1107 5 -0.35132407150959466 1107 6 -0.0782202494696449 1107 7 -0.47422170588582446 1107 8 -0.11256710906827294 1107 9 -0.26183778776518096 1107 10-11 0.02870648483573035 1107 12-13 -0.31763192960460884 1107 14-15 -0.3147534645478913 1107 16-17 -0.32431987541103524 1107 18-19 -0.19319882453681458 1107 20-21 -0.2823430478552922 1107 22-23 -0.09851295653651704 1107 24-25 -0.24440121856275 1107 26-27 -0.18286930270699742 1107 28-29 -0.24035029870214686 1107 30-31 -0.36626900363277315 1107 32-33 -0.8845861473657592 1107 34-35 -0.4896124292246711 1107 36-37 -0.6488597918961361 1107 38-39 -0.8532138152259989 1107 40-41 -0.41779880576141437 1107 42-43 -0.6562620331351638 1107 44-45 -1.0488869764371245 1107 46-47 -1.064555676072402 1107 48-49 -1.0829541462420949 1107 50-51 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2316 62-63 1.240670880749235 2316 64-65 1.282189120367363 2316 66-67 1.1117766087224794 2316 68-69 0.9639021250833011 2316 70-71 0.9411324849849123 2316 72-73 1.0962606732372002 2316 74-75 0.7013329735030318 2316 76-77 0.5654419245134363 2316 78-79 0.5277222692667252 2316 80-81 0.656036706167253 2316 82-83 0.09157592585900431 2316 84-85 0.19832047891020466 2316 86-87 0.3546453479361702 2316 88-89 0.3319878929603597 2316 90-91 0.4302678772856581 2316 92-93 0.5890292329050517 2316 94-95 0.7464525005189593 2316 96-97 0.4862462031257593 2316 98-99 -0.16243305166094402 2316 100-101 -1.063895084629003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 1.0 5 13.0 6 21.0 7 47.0 8 119.0 9 227.0 10 401.0 11 713.0 12 1162.0 13 1602.0 14 2141.0 15 3149.0 16 4320.0 17 5973.0 18 5179.0 19 3360.0 20 2675.0 21 2616.0 22 2658.0 23 2921.0 24 3353.0 25 3587.0 26 4242.0 27 5146.0 28 6074.0 29 7567.0 30 9058.0 31 11561.0 32 14695.0 33 19429.0 34 27280.0 35 39393.0 36 61913.0 37 106369.0 38 112818.0 39 12294.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86865513706557 21.38136139677921 15.16008895208492 21.589894514070302 2 14.795985820642699 22.760719214020707 40.80262929574206 21.640665669594533 3 18.64304542186893 26.84203567975806 30.890300030573208 23.624618867799803 4 11.973954933441304 18.872757620578245 38.50633361152197 30.646953834458486 5 12.041360218163788 39.91178415007024 34.79774398810015 13.249111643665811 6 28.997240148403996 39.313011791342 18.060708472083356 13.629039588170649 7 26.448922087902098 35.10754331892812 21.389882747622313 17.053651845547467 8 24.879359780533957 37.24477570008511 20.15497310384148 17.72089141553945 9 25.981234661752918 17.615331223506665 21.26986225531106 35.13357185942936 10-11 23.399761198469687 29.024094991778288 27.77513819915552 19.80100561059651 12-13 24.79421987408911 26.811582975690623 28.370543668562043 20.023653481658226 14-15 21.42572363474108 28.59348790705745 27.461143107394587 22.519645350806886 16-17 19.616326918468697 31.809458688987863 28.161228216590512 20.412986175952934 18-19 19.460775402616076 30.072466761966933 31.28744184893531 19.179315986481686 20-21 21.053577478288894 28.855322629956788 31.27711306302212 18.813986828732205 22-23 20.123821741312955 29.48574934463718 30.995407812296648 19.395021101753212 24-25 19.82141473819029 29.572232005908084 31.35632872495907 19.250024530942554 26-27 19.796830053039027 29.84160594119743 31.331140158342414 19.030423847421126 28-29 19.390400601563403 30.257998810094367 31.187819167396587 19.163781420945643 30-31 19.840978012080548 29.986737838887468 31.22629543632923 18.945988712702754 32-33 19.591124680840515 30.027949694681087 30.953615488221054 19.427310136257343 34-35 19.708253113096074 30.262309847051338 30.95588782112195 19.073549218730633 36-37 19.730563290668563 30.11068326984573 30.933680931408595 19.22507250807711 38-39 19.55290817296172 30.472087488948198 30.925108039100653 19.04989629898943 40-41 19.823832227464656 30.35000123945431 30.77823270341511 19.047933829665926 42-43 19.57384960880605 30.594011540583143 30.34175497023951 19.490383880371297 44-45 19.81970071309938 30.680832252253744 30.06410044537725 19.43536658926963 46-47 19.900161955363117 30.69994050619314 29.702592938415645 19.697304600028094 48-49 19.855644888077276 30.720391502301254 29.607877971591705 19.81608563802976 50-51 20.056643061947927 31.10658480759538 29.034320489832343 19.802451640624355 52-53 19.98351525768255 31.231149965708433 28.87587691392403 19.909457862684988 54-55 19.87124135480619 31.051738954396345 28.854289751365464 20.222729939432 56-57 20.1585881789111 31.019306566628934 28.60133778435148 20.220767470108495 58-59 20.085047223209195 31.03583262409003 28.770523297609508 20.108596855091264 60-61 20.122334140355804 30.89587757496633 28.931445782136983 20.050342502540882 62-63 20.01026681319771 30.738466877649333 29.160848117268905 20.09041819188405 64-65 19.968538518108428 30.749415390717317 29.09422744812884 20.18781864304542 66-67 19.847898298642384 30.75189429933648 28.953549383991206 20.44665801802993 68-69 20.010679964634235 30.677733616479784 28.88186760975368 20.4297188091323 70-71 19.908631559811933 30.629704761983454 28.913163831070644 20.548499847133968 72-73 19.73097644210509 30.470021731765563 28.980817378802026 20.818184447327322 74-75 19.646590261194337 30.74218524057808 28.676221482222093 20.93500301600549 76-77 19.440634270085358 30.368902917675445 28.992798770461327 21.197664041777873 78-79 19.39973227786913 30.45235950785401 28.65742309186009 21.49048512241677 80-81 19.313796779071403 30.44574908486957 28.573863213822392 21.666590922236637 82-83 19.191356845165856 30.238853914363773 28.77340122809645 21.796388012373924 84-85 19.17949851471445 30.178564788610196 28.710631669841057 21.9313050268343 86-87 19.037501755893604 30.241858850943228 28.362536254038556 22.358103139124616 88-89 19.068488113633173 30.02423133175234 28.285586798985303 22.621693755629188 90-91 19.094137626126983 29.881764992686495 28.60148831905065 22.422609062135873 92-93 18.598821247296367 29.92308979467888 28.59260315119063 22.885485806834122 94-95 18.22886110675007 30.320667487460856 28.396414671833814 23.054056733955264 96-97 18.19994050619314 30.4575239008106 28.441448178415317 22.90108741458094 98-99 18.30495659826228 30.754810135472916 27.85994934742461 23.0802839188402 100-101 18.116211514542968 30.529798724830275 27.420591139925705 23.933398620701055 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 507.0 1 347.5 2 210.0 3 250.0 4 241.0 5 282.0 6 471.0 7 823.5 8 1084.0 9 1114.0 10 1048.5 11 962.5 12 975.0 13 1018.0 14 1021.5 15 978.5 16 954.0 17 1012.0 18 1075.0 19 1172.0 20 1283.0 21 1483.0 22 1745.0 23 2098.5 24 2648.5 25 3285.5 26 4097.0 27 5076.5 28 6143.0 29 7287.5 30 8569.0 31 10029.0 32 11574.0 33 13157.5 34 14962.0 35 16565.0 36 17928.0 37 19141.5 38 19541.5 39 19547.0 40 19613.0 41 19453.5 42 19134.5 43 18807.0 44 18529.0 45 18205.0 46 17612.5 47 17379.0 48 17795.5 49 16780.5 50 15006.5 51 13520.5 52 11762.0 53 10329.0 54 8999.0 55 7747.5 56 6575.5 57 5556.5 58 4635.5 59 3713.5 60 2945.5 61 2407.5 62 1881.5 63 1247.0 64 784.0 65 541.5 66 409.0 67 336.5 68 240.0 69 149.0 70 98.0 71 81.0 72 75.5 73 69.0 74 56.5 75 41.5 76 31.5 77 22.5 78 21.0 79 18.5 80 8.5 81 3.0 82 3.5 83 2.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04709926376414011 2 0.0 3 0.0 4 0.0 5 0.009089331603605985 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0023756207600333827 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.065757182637724E-4 24-25 3.098635773956586E-4 26-27 0.0013427421687145205 28-29 0.0024789086191652687 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010948513067979938 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.098635773956586E-4 84-85 4.131514365275448E-4 86-87 0.0 88-89 0.0 90-91 0.009915634476661075 92-93 0.002685484337429041 94-95 0.0 96-97 0.0 98-99 4.131514365275448E-4 100-101 0.01446030027846407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 484084.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.46618977504001 #Duplication Level Percentage of deduplicated Percentage of total 1 87.4716732734532 68.63568915015074 2 8.800391651457442 13.81066402835875 3 1.995752574470695 4.697973007573266 4 0.74804654706927 2.3478544929160297 5 0.3215824939866878 1.2616676500745052 6 0.18224282083674848 0.8579939858948954 7 0.1066230283320476 0.585641194284434 8 0.06473839406110207 0.40638200913038064 9 0.05257558150315697 0.3712865000183818 >10 0.23396408505783017 3.219894953318618 >50 0.012654805799277745 0.6818423842858075 >100 0.00790925186965235 0.9381537239528128 >500 7.909251869650123E-4 0.37650270729741764 >1k 0.0010545669159533497 1.8084542127439696 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.6564976326422687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2622 0.5416415332876112 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1908 0.39414647044727774 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1034 0.21359929268474068 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 684 0.14129779129242032 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 626 0.12931639963312153 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 510 0.10535361631452392 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.098635773956586E-4 0.0 24-25 0.0 0.0 0.0 0.0012394543095826343 0.0 26-27 1.032878591318862E-4 0.0 0.0 0.00216904504176961 0.0 28-29 2.065757182637724E-4 0.0 0.0 0.005474256533989969 0.0 30-31 2.065757182637724E-4 0.0 0.0 0.012497830954958229 0.0 32-33 2.065757182637724E-4 0.0 0.0 0.020244420389849693 0.0 34-35 2.065757182637724E-4 0.0 0.0 0.027371282669949842 0.0 36-37 2.065757182637724E-4 0.0 0.0 0.03408499351352245 0.0 38-39 2.065757182637724E-4 0.0 0.0 0.04957817238330538 0.0 40-41 2.065757182637724E-4 0.0 0.0 0.07808562150370596 0.0 42-43 2.065757182637724E-4 0.0 0.0 0.13830244337759562 0.0 44-45 2.065757182637724E-4 0.0 0.0 0.18891349435221988 0.0 46-47 2.065757182637724E-4 0.0 0.0 0.3098635773956586 0.0 48-49 2.065757182637724E-4 0.0 0.0 0.43814709843746125 0.0 50-51 2.065757182637724E-4 0.0 0.0 0.5173688863916179 0.0 52-53 2.065757182637724E-4 0.0 0.0 0.6279901835218681 0.0 54-55 2.065757182637724E-4 0.0 0.0 0.7420199800034705 0.0 56-57 2.065757182637724E-4 0.0 0.0 1.0056105965080442 0.0 58-59 2.065757182637724E-4 0.0 0.0 1.3375777757579264 0.0 60-61 2.065757182637724E-4 0.0 0.0 1.6405210665917487 0.0 62-63 2.065757182637724E-4 0.0 0.0 1.949558341114352 0.0 64-65 2.065757182637724E-4 0.0 0.0 2.3244932697630993 0.0 66-67 2.065757182637724E-4 0.0 0.0 2.7962089224184234 0.0 68-69 2.065757182637724E-4 0.0 0.0 3.30913643086737 0.0 70-71 2.065757182637724E-4 0.0 0.0 3.6554606225365847 0.0 72-73 2.065757182637724E-4 0.0 0.0 4.070677816246767 0.0 74-75 2.065757182637724E-4 0.0 0.0 4.573276538782526 0.0 76-77 2.065757182637724E-4 0.0 0.0 5.156439791441155 0.0 78-79 2.065757182637724E-4 0.0 0.0 5.68238157014072 0.0 80-81 2.065757182637724E-4 0.0 0.0 6.4144859156675285 0.0 82-83 2.065757182637724E-4 0.0 0.0 7.190384313466258 0.0 84-85 2.065757182637724E-4 0.0 0.0 8.116256682724487 0.0 86-87 2.065757182637724E-4 0.0 0.0 8.894324125564985 0.0 88-89 2.065757182637724E-4 0.0 0.0 10.149065038299138 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCCCG 55 4.9577313E-5 43.183743 9 GGTATCA 2550 0.0 28.708313 1 GTATCAA 3525 0.0 27.105915 1 ATAGCCC 200 2.0469088E-8 23.751059 3 GTACATG 2635 0.0 23.272135 1 AGTACTT 2380 0.0 23.252087 12-13 TACATGG 2590 0.0 22.558922 2 TAGCCCT 360 0.0 22.431555 4 ATCAACG 4290 0.0 22.256237 3 GAGTACT 2335 0.0 22.174437 12-13 TCAACGC 4330 0.0 22.050636 4 GTCCTAG 195 4.179983E-7 21.939919 1 ATGGGCG 65 2.9422122E-4 21.921791 18-19 CAACGCA 4320 0.0 21.881763 5 GTATACT 175 4.039677E-6 21.715254 4 AACGCAG 4375 0.0 21.604446 6 TATCAAC 4415 0.0 21.518515 2 GTAGACC 380 0.0 21.250946 3 TCTATAC 255 1.1868906E-8 20.49111 3 GTACTTT 2540 0.0 20.382662 14-15 >>END_MODULE