FastQCFastQC Report
Thu 26 May 2016
SRR937787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953172
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158611.664022862610316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127961.3424649486136815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85150.8933329976121833No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28960.3038276407615834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23530.24685995811878653No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14030.14719274170873672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13940.14624852597432572No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.14257657589606074No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.13250494139567676No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.12033504970771276No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11330.11886626967640677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11270.11823679252013278No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.11813187966075378No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10890.1142501038637308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74550.039.0900151
GTATCAA118800.034.413991
GGTAGCG600.00394783131.671227
ATGGGCG1205.3568256E-627.723985
TATCAAC147300.027.6162132
ATCAACG148150.027.3950543
TCAACGC148850.027.2357274
GTACATG79250.026.9339541
CAACGCA150550.026.8650465
AACGCAG152600.026.5227366
TACATGG79700.026.4142632
GTACACG1109.4217845E-525.930541
TGCGACG559.4705625E-525.91281732-33
ACATGGG80900.025.3776383
TAGGTCG950.001222369725.0141165
GAGTACT94900.024.90413912-13
GTGTAAG4250.024.6085911
ACGCAGA167250.024.0019577
CGCAGAG168100.023.8240688
GGTGCGA601.7103313E-423.75216930-31
Produced by FastQC (version 0.11.5)