##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937787_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 953172 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.870365474436934 33.0 31.0 34.0 30.0 34.0 2 31.95744524597869 33.0 31.0 34.0 30.0 34.0 3 31.926588275778137 33.0 31.0 34.0 30.0 34.0 4 35.55521983440554 37.0 35.0 37.0 33.0 37.0 5 35.571352284792255 37.0 35.0 37.0 33.0 37.0 6 35.67053270553478 37.0 35.0 37.0 33.0 37.0 7 35.63258362604021 37.0 35.0 37.0 33.0 37.0 8 35.62648189413873 37.0 35.0 37.0 33.0 37.0 9 37.24475750441683 39.0 37.0 39.0 34.0 39.0 10-11 37.13494888645491 39.0 37.0 39.0 34.0 39.0 12-13 37.01577574666482 39.0 37.0 39.0 33.0 39.0 14-15 38.06045446152426 40.0 38.0 41.0 33.0 41.0 16-17 38.019578837817306 40.0 38.0 41.0 33.0 41.0 18-19 38.10419630454945 40.0 38.0 41.0 33.5 41.0 20-21 38.09513498088488 40.0 38.0 41.0 33.5 41.0 22-23 38.00512132123059 40.0 38.0 41.0 33.0 41.0 24-25 37.99835968744361 40.0 38.0 41.0 33.0 41.0 26-27 37.896359733605266 40.0 38.0 41.0 33.0 41.0 28-29 37.758462795801805 40.0 37.0 41.0 33.0 41.0 30-31 37.407137431649275 39.5 36.5 41.0 33.0 41.0 32-33 37.11376068537473 39.0 35.5 41.0 32.0 41.0 34-35 37.020781663750085 39.0 35.0 41.0 32.0 41.0 36-37 36.82897420402614 39.0 35.0 41.0 31.0 41.0 38-39 36.643868053195014 39.0 35.0 41.0 31.0 41.0 40-41 36.35432692105937 39.0 35.0 41.0 30.5 41.0 42-43 35.967716214911896 39.0 35.0 41.0 30.0 41.0 44-45 35.52511771222822 39.0 35.0 41.0 28.0 41.0 46-47 34.870570054512726 39.0 35.0 40.5 23.5 41.0 48-49 34.29751660770564 38.5 34.5 40.0 20.0 41.0 50-51 33.30581993596119 37.5 33.5 39.5 14.5 40.5 52-53 33.38048222146685 38.0 33.5 39.5 12.5 40.5 54-55 33.64715602220795 38.0 34.0 40.0 8.5 41.0 56-57 33.4309699613501 38.0 34.0 40.0 7.5 41.0 58-59 33.20174795315012 38.0 33.0 40.0 4.0 41.0 60-61 32.88841678102168 38.0 33.0 40.0 2.0 41.0 62-63 32.53269346980398 37.0 33.0 40.0 2.0 41.0 64-65 32.16119441192146 36.5 32.0 40.0 2.0 41.0 66-67 31.726384639918084 36.0 31.5 39.0 2.0 41.0 68-69 31.246267725027593 35.5 31.0 39.0 2.0 40.0 70-71 30.78966440474542 35.0 30.5 38.5 2.0 40.0 72-73 30.339210027151445 35.0 30.0 38.0 2.0 40.0 74-75 29.83203503669852 35.0 29.0 37.0 2.0 39.0 76-77 29.36174635847465 34.5 29.0 36.5 2.0 39.0 78-79 28.827461885158186 34.0 28.5 36.0 2.0 39.0 80-81 28.280824971778443 34.0 27.5 35.5 2.0 37.5 82-83 27.80255976885599 34.0 26.0 35.0 2.0 37.0 84-85 27.311631059242195 34.0 26.0 35.0 2.0 36.5 86-87 26.805113347853272 33.0 25.0 35.0 2.0 36.0 88-89 26.366612741456947 33.0 24.5 35.0 2.0 36.0 90-91 25.85099698690268 33.0 22.0 35.0 2.0 35.0 92-93 25.299557162820562 32.0 19.5 35.0 2.0 35.0 94-95 24.628770043601783 32.0 13.0 34.0 2.0 35.0 96-97 23.887209758574528 31.5 2.0 34.0 2.0 35.0 98-99 22.955345939662518 31.0 2.0 34.0 2.0 35.0 100-101 20.34344011364161 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22066814622321473 1101 2 0.3657245395768456 1101 3 0.45886021841740643 1101 4 0.19985406325967858 1101 5 0.20561142384990916 1101 6 0.29202609111035827 1101 7 0.3022709783000721 1101 8 0.2557338390983048 1101 9 0.3390377457876781 1101 10-11 0.5331130078418624 1101 12-13 0.29901684395569106 1101 14-15 0.4780997123518347 1101 16-17 0.4837087392066266 1101 18-19 0.3964465119202316 1101 20-21 0.5336976530809281 1101 22-23 0.4960859899889343 1101 24-25 0.39859296551777135 1101 26-27 0.39433745419590593 1101 28-29 0.2904601669570539 1101 30-31 0.4333032241837955 1101 32-33 0.6131209836111537 1101 34-35 0.6133971120293396 1101 36-37 0.574661178246707 1101 38-39 0.6710514940065977 1101 40-41 0.814354866820139 1101 42-43 0.7260271521386699 1101 44-45 0.8868566596835876 1101 46-47 1.0884149670371883 1101 48-49 1.2669025941613299 1101 50-51 1.2657598516332769 1101 52-53 1.3725561791287149 1101 54-55 1.2848672304724502 1101 56-57 1.086576743555419 1101 58-59 1.104035586562233 1101 60-61 1.410733368586996 1101 62-63 1.4288644377013355 1101 64-65 1.3426393730549364 1101 66-67 1.2858543751293858 1101 68-69 1.2676663056506179 1101 70-71 1.5875878724992845 1101 72-73 1.5735848648653068 1101 74-75 1.3930485431447224 1101 76-77 1.4443821036065643 1101 78-79 1.6098333159092242 1101 80-81 1.5623227605603738 1101 82-83 1.459002850128197 1101 84-85 1.6264626537091083 1101 86-87 1.7114824575517353 1101 88-89 1.6799690937711453 1101 90-91 1.8220520275126795 1101 92-93 1.7991535415898277 1101 94-95 2.021362845324127 1101 96-97 2.3640361250970052 1101 98-99 2.497418309563521 1101 100-101 2.518367148477399 1102 1 0.10970587329375547 1102 2 0.14860726815565073 1102 3 0.23836213314675803 1102 4 0.08846743108711763 1102 5 0.08314671468937718 1102 6 0.23300181060378833 1102 7 0.26549615967530116 1102 8 0.27397745198042145 1102 9 0.25698814177109597 1102 10-11 0.19784108559674252 1102 12-13 0.3609349477345276 1102 14-15 0.4542806325467268 1102 16-17 0.48027615864228324 1102 18-19 0.43638613020142714 1102 20-21 0.4765139668818037 1102 22-23 0.3572629989209304 1102 24-25 0.5109305714158552 1102 26-27 0.3602040162213811 1102 28-29 0.44741301195345073 1102 30-31 0.6566823508217965 1102 32-33 0.7142316033290328 1102 34-35 0.7194562462342304 1102 36-37 0.9058748456819927 1102 38-39 0.950796264929302 1102 40-41 0.874764066724012 1102 42-43 1.0282706320889758 1102 44-45 1.1696850651665187 1102 46-47 1.479815429230868 1102 48-49 1.6596496300375847 1102 50-51 1.6563582492936817 1102 52-53 1.458986141958924 1102 54-55 1.591898172907861 1102 56-57 1.5561494189103726 1102 58-59 1.5689901596721754 1102 60-61 1.6730794105423215 1102 62-63 1.5966735936354581 1102 64-65 1.8337118515685589 1102 66-67 1.7656033640807074 1102 68-69 1.5625761676571734 1102 70-71 1.4740679403967647 1102 72-73 1.6778705097635402 1102 74-75 1.7213977856890388 1102 76-77 1.6844984956327025 1102 78-79 2.036550181377116 1102 80-81 1.8384655906786342 1102 82-83 2.1440305101704276 1102 84-85 1.7914214448476713 1102 86-87 2.0991295133677603 1102 88-89 1.990577691391664 1102 90-91 1.9810058797231846 1102 92-93 2.132414231096906 1102 94-95 2.1814344751192607 1102 96-97 2.274209371036193 1102 98-99 2.195663737476135 1102 100-101 2.146936495424317 1103 1 0.3535566406152135 1103 2 0.4343955563208084 1103 3 0.4542044372433338 1103 4 0.32583683335818847 1103 5 0.2791362504694632 1103 6 0.20387803867348708 1103 7 0.24198036766944142 1103 8 0.20657918289579413 1103 9 0.26607189380079177 1103 10-11 0.31175975553644975 1103 12-13 0.38004007651441896 1103 14-15 0.592183906852533 1103 16-17 0.28958092221323994 1103 18-19 0.40797992821026696 1103 20-21 0.30357004394745246 1103 22-23 0.38739000196615336 1103 24-25 0.5432171182779513 1103 26-27 0.4173136991784716 1103 28-29 0.43791921995852334 1103 30-31 0.4978465362910711 1103 32-33 0.6066049966557685 1103 34-35 0.6615763715424379 1103 36-37 0.5252087767624758 1103 38-39 0.5820691929973592 1103 40-41 0.34336115598698314 1103 42-43 0.39542315055443567 1103 44-45 0.44613605992347516 1103 46-47 0.5937757865670292 1103 48-49 0.6203978709661229 1103 50-51 0.4731127556328971 1103 52-53 0.42051072522164645 1103 54-55 0.6083400921135862 1103 56-57 0.5886806229517205 1103 58-59 0.34274514165775116 1103 60-61 0.6504014668654108 1103 62-63 0.6019309893896363 1103 64-65 0.7090275823417826 1103 66-67 0.8067857279884443 1103 68-69 0.8091420829483802 1103 70-71 0.8530446446595406 1103 72-73 0.8616688739774361 1103 74-75 0.9094175113449658 1103 76-77 1.0745073251599564 1103 78-79 1.2477638176662005 1103 80-81 1.1775249043264004 1103 82-83 1.2043590068545704 1103 84-85 1.1120009406625293 1103 86-87 1.1490928831308658 1103 88-89 1.2088166790850003 1103 90-91 1.202817682313544 1103 92-93 1.0395969011678154 1103 94-95 1.1514304840635958 1103 96-97 1.6033833045222465 1103 98-99 1.8869836014522114 1103 100-101 1.8359307493976011 1104 1 0.33268518105224487 1104 2 0.27243533283544963 1104 3 0.18117720451072472 1104 4 0.0423428143788982 1104 5 0.27692139426847007 1104 6 0.11452328597384565 1104 7 0.1831518478357239 1104 8 0.22694010446988244 1104 9 0.37959463622301115 1104 10-11 0.24820535066921678 1104 12-13 0.34092104996037875 1104 14-15 0.43427087101488837 1104 16-17 0.39173851332230214 1104 18-19 0.2521923665518244 1104 20-21 0.4350191840621065 1104 22-23 0.27106233050417217 1104 24-25 0.33205739411996404 1104 26-27 0.23296630913350924 1104 28-29 0.3077252155227512 1104 30-31 0.3570736393440015 1104 32-33 0.468871088449589 1104 34-35 0.4639095453010924 1104 36-37 0.38294213958682377 1104 38-39 0.48844985739361135 1104 40-41 0.47216292181214214 1104 42-43 0.5886087561100624 1104 44-45 0.6197757867477804 1104 46-47 0.7241970334310892 1104 48-49 0.5943066528255159 1104 50-51 0.5164774282220037 1104 52-53 0.6555059076821621 1104 54-55 0.5419792432570674 1104 56-57 0.44932268885808213 1104 58-59 0.5395401144492595 1104 60-61 0.6468776942775136 1104 62-63 0.7993811778493551 1104 64-65 0.8171048387075999 1104 66-67 0.8293304492983289 1104 68-69 0.7142645817667201 1104 70-71 0.6189508571645383 1104 72-73 0.7797737327235446 1104 74-75 0.8055275754576314 1104 76-77 0.6725268316499964 1104 78-79 0.6346963175753046 1104 80-81 0.724308060511639 1104 82-83 0.8556668627768111 1104 84-85 0.9445134238465904 1104 86-87 0.8411286419993793 1104 88-89 1.0081608574369731 1104 90-91 1.266832631835424 1104 92-93 1.2337301076493752 1104 94-95 1.124988555108672 1104 96-97 1.2891191458040119 1104 98-99 1.202458204838429 1104 100-101 1.316358359049648 1105 1 0.24058303773233902 1105 2 0.24125066268230455 1105 3 0.19644727350572566 1105 4 0.17006454346166322 1105 5 0.18227702240346844 1105 6 0.1627379652773513 1105 7 0.08160964600698861 1105 8 0.12132031833434098 1105 9 0.25471485983894127 1105 10-11 0.17034928839338193 1105 12-13 0.3256960648213507 1105 14-15 0.38723463361331767 1105 16-17 0.3274898441309375 1105 18-19 0.47401203446747786 1105 20-21 0.39764132791633955 1105 22-23 0.4327159228526085 1105 24-25 0.32414935182796967 1105 26-27 0.3258528280587143 1105 28-29 0.4336415031510725 1105 30-31 0.6070414962244612 1105 32-33 0.38041280604431194 1105 34-35 0.49600799842389875 1105 36-37 0.6731481728086166 1105 38-39 0.7023886168194835 1105 40-41 0.7574840357507355 1105 42-43 0.7724970383149667 1105 44-45 0.7369566598383912 1105 46-47 0.9970808473249306 1105 48-49 1.0811167028518938 1105 50-51 0.8588513270070806 1105 52-53 1.0067371143933102 1105 54-55 0.8858855757020834 1105 56-57 0.9169971466829736 1105 58-59 0.8463056984354722 1105 60-61 1.1241036679218297 1105 62-63 1.1508787132652287 1105 64-65 1.0723533509403538 1105 66-67 0.9797230962883994 1105 68-69 1.1905876259622552 1105 70-71 1.250136111738044 1105 72-73 1.2707786685708875 1105 74-75 1.1314366830644431 1105 76-77 1.3287453146951265 1105 78-79 1.3384164870906012 1105 80-81 1.4390466409378995 1105 82-83 1.2038615882996169 1105 84-85 1.3636425874241773 1105 86-87 1.3091896521449193 1105 88-89 1.4243011283408578 1105 90-91 1.4132228342786384 1105 92-93 1.4329548323039027 1105 94-95 1.9443909769466217 1105 96-97 2.049042603435204 1105 98-99 2.0417701584053916 1105 100-101 2.2035222338567912 1106 1 0.08554509287954914 1106 2 0.2311233839148059 1106 3 0.13063942707077558 1106 4 0.3379873871973871 1106 5 0.13632014768896283 1106 6 0.12905087794708692 1106 7 0.2306586888208244 1106 8 0.24142018960214529 1106 9 0.17445059548114727 1106 10-11 0.13170432452144354 1106 12-13 -0.13536828570395443 1106 14-15 0.07360368494009606 1106 16-17 0.06661119498846091 1106 18-19 0.3539900254147028 1106 20-21 0.16646776265742602 1106 22-23 0.20519409547833334 1106 24-25 0.10790586629995857 1106 26-27 0.1936116420614553 1106 28-29 0.23415137088912985 1106 30-31 0.2408529035580429 1106 32-33 0.16268474761650964 1106 34-35 0.31678085877022966 1106 36-37 0.14657185226545266 1106 38-39 0.26888749041277293 1106 40-41 0.3256945803709783 1106 42-43 0.6327616141216197 1106 44-45 0.564088267745035 1106 46-47 0.6329601453191955 1106 48-49 0.4204506175564333 1106 50-51 0.5483355157866185 1106 52-53 0.507170547390281 1106 54-55 0.25749402738598093 1106 56-57 0.44250545717672907 1106 58-59 0.2629600369180949 1106 60-61 0.25352105092087385 1106 62-63 0.4261294295976086 1106 64-65 0.6125166280052596 1106 66-67 0.7041641753548795 1106 68-69 0.44641626160861847 1106 70-71 0.3609554558267476 1106 72-73 0.6130817290750166 1106 74-75 0.4972794377257941 1106 76-77 0.2608040435685126 1106 78-79 0.5792890889343774 1106 80-81 0.26955357586585293 1106 82-83 0.04990333282849946 1106 84-85 0.48493699484081176 1106 86-87 0.634549894659763 1106 88-89 0.6286524582274886 1106 90-91 0.651790065705903 1106 92-93 0.4448885144599579 1106 94-95 0.3398382434637952 1106 96-97 0.5937900184096705 1106 98-99 1.0912541914605 1106 100-101 1.1190339443339319 1107 1 0.10917196860097889 1107 2 0.15445928233727813 1107 3 0.1991393308799303 1107 4 0.1135260559161253 1107 5 0.1935777467655342 1107 6 -0.001371880806281922 1107 7 0.20714002779735097 1107 8 0.21357294066024224 1107 9 0.17066308105251693 1107 10-11 0.20926420332099127 1107 12-13 0.2170929974818847 1107 14-15 0.17480246331638938 1107 16-17 0.3805179860619532 1107 18-19 0.41846193768864737 1107 20-21 0.35218222014106715 1107 22-23 0.28565172341214407 1107 24-25 0.35995593363354317 1107 26-27 0.4351949433311759 1107 28-29 0.31231244245510936 1107 30-31 0.4446332036734759 1107 32-33 0.29057648482312715 1107 34-35 0.18229403691567114 1107 36-37 0.23557844133816985 1107 38-39 0.29499729225963023 1107 40-41 0.2816692340839495 1107 42-43 0.401073945787644 1107 44-45 0.6862867094533911 1107 46-47 0.4295585966466291 1107 48-49 0.30102968642908223 1107 50-51 0.02978270701401442 1107 52-53 0.016044152623059915 1107 54-55 -0.034270834099203284 1107 56-57 0.02990084963536077 1107 58-59 0.005727687937721271 1107 60-61 0.18161839605361507 1107 62-63 0.20157190747372056 1107 64-65 0.3832637903754019 1107 66-67 0.3297713800489923 1107 68-69 0.3401193685728323 1107 70-71 0.43923676594602057 1107 72-73 0.44551728121045286 1107 74-75 0.5286075927854341 1107 76-77 0.6114076411060303 1107 78-79 0.4919778634630454 1107 80-81 0.5420141376203738 1107 82-83 0.4752712628015665 1107 84-85 0.5952498555564709 1107 86-87 0.555312688041834 1107 88-89 0.606763029601094 1107 90-91 0.753511881789013 1107 92-93 0.812505867287971 1107 94-95 0.8972942361532894 1107 96-97 0.9015526194100545 1107 98-99 1.0081933988479577 1107 100-101 0.6708086653804877 1108 1 -0.007738284371221482 1108 2 -0.06488012948000943 1108 3 0.1226107709442914 1108 4 0.04467381670990278 1108 5 0.04488953723661382 1108 6 0.17959710104766202 1108 7 0.26842768311155396 1108 8 0.15885541138518988 1108 9 0.3687166253450016 1108 10-11 0.323671551135412 1108 12-13 0.24073651227584492 1108 14-15 0.23142510566911767 1108 16-17 0.31020199428578366 1108 18-19 0.4761628405222922 1108 20-21 0.43535912190203874 1108 22-23 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2316 80-81 -0.4939346785880794 2316 82-83 -0.6881768502638366 2316 84-85 -0.6876427996121315 2316 86-87 -0.8270056499288962 2316 88-89 -0.715725996344883 2316 90-91 -0.6694142228989435 2316 92-93 -0.8077209214577259 2316 94-95 -0.7858148866255306 2316 96-97 -0.6194280391336378 2316 98-99 -0.9373632393246751 2316 100-101 -0.9191421648305571 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3301.0 3 654.0 4 524.0 5 550.0 6 696.0 7 814.0 8 1085.0 9 1440.0 10 1835.0 11 2780.0 12 4226.0 13 6133.0 14 7106.0 15 9795.0 16 13739.0 17 20237.0 18 16835.0 19 8940.0 20 7756.0 21 7709.0 22 8143.0 23 8620.0 24 9321.0 25 10633.0 26 12144.0 27 13785.0 28 16126.0 29 18924.0 30 23076.0 31 28170.0 32 35691.0 33 46635.0 34 64050.0 35 91606.0 36 124907.0 37 172161.0 38 137049.0 39 15974.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.906878959053536 19.09295802496143 15.453915381031461 19.546247634953573 2 15.089985185590175 21.2397000949426 41.90974460125686 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35.530817014600565 18.837030541438136 22-23 20.131558553801618 25.417595956570967 35.5146624214865 18.93618306814092 24-25 19.451598768020226 25.61338911078676 36.08637308895495 18.848639032238065 26-27 19.363011143627222 25.605071112208606 36.058697125564144 18.97322061860003 28-29 18.936231028145023 25.880431732847697 36.189729583746235 18.993607655261048 30-31 19.012138873504973 25.801490377491536 36.13708278205199 19.049287966951496 32-33 18.766091962478534 26.03598083992425 35.8508956851997 19.34703151239751 34-35 19.356993030509386 25.815962151210204 35.74170639024103 19.08533842803938 36-37 19.57640101628451 25.545338772010567 35.66704703783327 19.211213173871652 38-39 19.311780302612412 25.97296230977714 35.63326540551019 19.08199198210026 40-41 19.11545822932981 26.193284476413286 35.617619383612634 19.07363791064427 42-43 19.176554901243755 26.377312333707426 34.95895489451849 19.487177870530328 44-45 19.215222875085182 26.629102672756627 34.49174329472726 19.66393115743093 46-47 19.62445448804103 26.961925288111228 33.57663543250574 19.836984791342005 48-49 19.89073501665298 27.30983579253104 32.8572036549862 19.942225535829785 50-51 19.829277993874562 27.681323825892136 32.52035638717935 19.96904179305395 52-53 19.975385029326183 28.08618603011077 32.032989526025176 19.905439414537863 54-55 19.60445904312776 27.923344303361198 32.18978148208859 20.282415171422446 56-57 19.833171614798534 28.250765820879604 31.74882441044532 20.167238153876543 58-59 20.107224098892036 27.702010517523075 31.986351386138097 20.204413997446796 60-61 20.00496965672595 27.644057005009582 32.52514247049726 19.825830867767205 62-63 20.01440539146205 27.581176114579076 32.569581403070416 19.834837090888456 64-65 19.901327886456148 27.67641935070366 32.59762665775665 19.824626105083542 66-67 19.74535945020504 27.90040272207764 32.180309800761314 20.173928026956006 68-69 19.66338121407631 28.023527062952493 32.190149946935385 20.12294177603581 70-71 19.82262649590925 28.157741689918414 31.905585726061382 20.114046088110953 72-73 19.884255379955146 28.099696296455846 31.69107870182364 20.32496962176537 74-75 19.62551865367016 28.530653758420076 31.4442554345028 20.399572153406957 76-77 19.703333622277032 28.337917031129745 31.588376057599614 20.37037328899361 78-79 19.71529968661425 28.16962233471069 31.42310753623112 20.691970442443935 80-81 19.646028020198752 28.537198106209456 31.308064580527073 20.508709293064715 82-83 19.726522380289186 28.40193476905231 31.28265395593236 20.588888894726143 84-85 19.646444376268665 28.25666009657613 30.952218351145422 21.144677176009782 86-87 19.730273639929546 28.1488606259715 31.113245042505568 21.00762069159339 88-89 19.968869395926543 27.819497129901194 30.992339223773147 21.219294250399116 90-91 19.501030741524488 27.89253810381385 31.45936608870891 21.14706506595275 92-93 19.777504843474862 27.88733089863794 31.412561095024564 20.92260316286263 94-95 19.368580646076403 27.742903808258486 31.373386476955073 21.51512906871004 96-97 19.493236782990778 27.5953858406373 31.312318363967613 21.59905901240431 98-99 19.518455594885744 27.95956170129159 30.92634677879448 21.595635925028184 100-101 19.634102142545736 27.512356800310954 31.568486351053938 21.28505470608937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2002.0 1 1329.5 2 886.0 3 1106.0 4 882.5 5 788.5 6 1528.5 7 3702.5 8 6344.0 9 7202.0 10 5702.5 11 4145.0 12 3891.5 13 4043.0 14 4133.0 15 4131.0 16 4177.5 17 4246.5 18 4361.0 19 4466.5 20 4705.5 21 5197.5 22 5820.5 23 6578.0 24 7431.5 25 8412.5 26 9799.5 27 11406.5 28 12372.5 29 13161.5 30 14669.5 31 16504.5 32 18356.0 33 20742.5 34 23785.5 35 25641.0 36 27208.0 37 28814.5 38 29201.5 39 29123.5 40 29092.5 41 30053.5 42 30903.5 43 31594.5 44 32616.0 45 33111.5 46 32141.5 47 32071.0 48 36681.0 49 36124.0 50 29998.0 51 26314.5 52 23835.0 53 22433.0 54 21132.0 55 19179.0 56 16669.5 57 14408.0 58 12944.5 59 11486.5 60 9945.0 61 8458.5 62 6915.5 63 5224.5 64 3543.0 65 2582.5 66 1859.0 67 1338.0 68 922.0 69 587.0 70 439.5 71 332.0 72 275.0 73 244.5 74 201.0 75 155.5 76 115.0 77 70.5 78 42.5 79 37.0 80 27.5 81 12.5 82 10.5 83 9.5 84 10.0 85 9.5 86 4.5 87 2.5 88 2.5 89 1.5 90 0.5 91 0.5 92 1.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24602065524375452 2 0.21727453177390857 3 0.2170647060551506 4 0.2187433118052146 5 0.2254577348054706 6 0.1635591477718607 7 0.1651328406625457 8 0.1656574049594407 9 0.1615658034436597 10-11 0.17258165367845468 12-13 0.1655524921000617 14-15 0.16523775352192469 16-17 0.16964409361584268 18-19 0.1653951228109932 20-21 0.16056913127955919 22-23 0.1578413969357052 24-25 0.1608314134280067 26-27 0.1581036790841527 28-29 0.16418862492813469 30-31 0.16796548786577867 32-33 0.1689097036001897 34-35 0.16744092356888368 36-37 0.1542219032871297 38-39 0.14965819390414323 40-41 0.1554284011699882 42-43 0.1611461520061437 44-45 0.16277230132651818 46-47 0.1618805420217967 48-49 0.16161825987334918 50-51 0.1643459942172032 52-53 0.18029274884281118 54-55 0.1561103347559517 56-57 0.1485041524509742 58-59 0.1497106503338327 60-61 0.14645835169308374 62-63 0.15159908180265472 64-65 0.15044504034948572 66-67 0.14677309027122074 68-69 0.1575791147872577 70-71 0.1522810153886182 72-73 0.14913362960724824 74-75 0.1499204760525907 76-77 0.14997293248228022 78-79 0.15416944685744022 80-81 0.1500253889119697 82-83 0.15007784534165922 84-85 0.14519939738053572 86-87 0.14220938088823423 88-89 0.14430763807581423 90-91 0.1500253889119697 92-93 0.14436009450550372 94-95 0.14808450101345821 96-97 0.14750748028687372 98-99 0.14698291598997873 100-101 0.13292459283319275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 953172.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.33126233133483 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86082165603766 37.423100937846 2 13.025587000006686 12.851372986339088 3 4.451214178901936 6.5875204305710575 4 2.102057422638577 4.147885846068529 5 1.1263287043559902 2.7781608392948915 6 0.690635770221718 2.044196061372663 7 0.47345474430851686 1.634928413544895 8 0.3277029380458839 1.2932799682792528 9 0.23483828987015676 1.0426382354724077 >10 1.5157952294322254 14.149838855263475 >50 0.12564247191573277 4.2657736489971425 >100 0.05992872208429774 5.160325515839214 >500 0.002996436090279673 0.9762321394756031 >1k 0.0023543426423626003 1.719967721399262 >5k 2.1403114930569094E-4 0.8990500397614926 >10k+ 4.280622986113819E-4 3.025728360475055 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15861 1.664022862610316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12796 1.3424649486136815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8515 0.8933329976121833 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2896 0.3038276407615834 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2353 0.24685995811878653 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1403 0.14719274170873672 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1394 0.14624852597432572 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.14257657589606074 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.13250494139567676 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1147 0.12033504970771276 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1133 0.11886626967640677 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1127 0.11823679252013278 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1126 0.11813187966075378 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1089 0.1142501038637308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0491285937899981E-4 0.0 12-13 0.0 0.0 0.0 1.0491285937899981E-4 0.0 14-15 0.0 0.0 0.0 1.0491285937899981E-4 0.0 16-17 0.0 0.0 0.0 2.098257187579996E-4 0.0 18-19 0.0 0.0 0.0 3.147385781369994E-4 1.0491285937899981E-4 20-21 0.0 0.0 0.0 3.671950078264993E-4 1.0491285937899981E-4 22-23 0.0 0.0 0.0 4.721078672054991E-4 1.0491285937899981E-4 24-25 0.0 0.0 0.0 7.343900156529987E-4 1.0491285937899981E-4 26-27 0.0 0.0 0.0 0.0013114107422374974 1.0491285937899981E-4 28-29 0.0 0.0 0.0 0.003671950078264993 1.0491285937899981E-4 30-31 0.0 0.0 0.0 0.008078290172182985 1.0491285937899981E-4 32-33 0.0 0.0 0.0 0.013009194562995974 1.0491285937899981E-4 34-35 0.0 0.0 0.0 0.018831858258530464 1.0491285937899981E-4 36-37 0.0 0.0 0.0 0.02580856340723395 1.0491285937899981E-4 38-39 0.0 0.0 0.0 0.03283772498562694 1.0491285937899981E-4 40-41 0.0 0.0 0.0 0.040181625142156924 1.0491285937899981E-4 42-43 0.0 0.0 0.0 0.05544644618180139 1.0491285937899981E-4 44-45 0.0 0.0 0.0 0.07396356586219487 1.0491285937899981E-4 46-47 0.0 0.0 0.0 0.09263805483165682 1.0491285937899981E-4 48-49 0.0 0.0 0.0 0.11613853533255278 1.0491285937899981E-4 50-51 0.0 0.0 0.0 0.14635343883370472 1.0491285937899981E-4 52-53 0.0 0.0 0.0 0.20132777714830064 1.0491285937899981E-4 54-55 0.0 0.0 0.0 0.25158103679084154 1.0491285937899981E-4 56-57 0.0 0.0 0.0 0.3292690091609909 1.0491285937899981E-4 58-59 0.0 0.0 0.0 0.4372767978916712 1.0491285937899981E-4 60-61 0.0 0.0 0.0 0.5462288023567625 1.0491285937899981E-4 62-63 0.0 0.0 0.0 0.6948378676671156 1.0491285937899981E-4 64-65 0.0 0.0 0.0 0.797390187710088 1.0491285937899981E-4 66-67 0.0 0.0 0.0 0.9668769120368621 1.0491285937899981E-4 68-69 0.0 0.0 0.0 1.1668932784429251 1.0491285937899981E-4 70-71 0.0 0.0 0.0 1.3326031398320555 1.0491285937899981E-4 72-73 0.0 0.0 0.0 1.603068491311117 1.0491285937899981E-4 74-75 0.0 0.0 0.0 1.870648739157256 1.0491285937899981E-4 76-77 0.0 0.0 0.0 2.069353694821082 1.0491285937899981E-4 78-79 0.0 0.0 0.0 2.295860558220342 1.0491285937899981E-4 80-81 0.0 0.0 0.0 2.6478956578665764 1.0491285937899981E-4 82-83 0.0 0.0 0.0 2.9784760777698045 1.0491285937899981E-4 84-85 0.0 0.0 0.0 3.389157465808899 1.0491285937899981E-4 86-87 0.0 0.0 0.0 3.909210509750601 1.0491285937899981E-4 88-89 0.0 0.0 0.0 4.569532046682026 1.0491285937899981E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7455 0.0 39.090015 1 GTATCAA 11880 0.0 34.41399 1 GGTAGCG 60 0.003947831 31.67122 7 ATGGGCG 120 5.3568256E-6 27.72398 5 TATCAAC 14730 0.0 27.616213 2 ATCAACG 14815 0.0 27.395054 3 TCAACGC 14885 0.0 27.235727 4 GTACATG 7925 0.0 26.933954 1 CAACGCA 15055 0.0 26.865046 5 AACGCAG 15260 0.0 26.522736 6 TACATGG 7970 0.0 26.414263 2 GTACACG 110 9.4217845E-5 25.93054 1 TGCGACG 55 9.4705625E-5 25.912817 32-33 ACATGGG 8090 0.0 25.377638 3 TAGGTCG 95 0.0012223697 25.014116 5 GAGTACT 9490 0.0 24.904139 12-13 GTGTAAG 425 0.0 24.608591 1 ACGCAGA 16725 0.0 24.001957 7 CGCAGAG 16810 0.0 23.824068 8 GGTGCGA 60 1.7103313E-4 23.752169 30-31 >>END_MODULE