FastQCFastQC Report
Thu 26 May 2016
SRR937787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953172
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132991.3952361168813183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117451.2322015334063527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82220.8625935298141364No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.2835794589014365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24320.2551480740097275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.24035536083728856No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22900.24025044797790956No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14230.14929099889631672No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.14488465880239873No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12190.12788877558300077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.10973885091043381No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.10931919947291781No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.1081651580197488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG702.0458674E-433.9488751
GGTATCA92900.031.8732071
CGCGTAA300.00395220831.6656710-11
GGGTCGC600.0039522431.6640076
GTATCAA131100.028.350291
CATGGGT7150.023.920354
GAGTACT93450.023.66030112-13
GTATAGA3500.023.0852341
TATCAAC162000.022.9037782
ATCAACG163400.022.7075393
AGTACTT94550.022.70684812-13
TCAACGC164000.022.653434
CCGTCCA1050.002196350622.6183349
GTACTTT97750.022.61830514-15
GTACATG88200.022.3631481
CAACGCA166100.022.281225
AACGCAG168950.021.983956
GCGGTAT1302.9291236E-421.9361951
TACATGG89450.021.9351582
ACATGGG88100.021.3545483
Produced by FastQC (version 0.11.5)