##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937787_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 953172 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13784815332385 33.0 31.0 34.0 31.0 34.0 2 32.406977964103014 34.0 31.0 34.0 31.0 34.0 3 32.45624084635302 34.0 31.0 34.0 31.0 34.0 4 36.11226934907866 37.0 35.0 37.0 35.0 37.0 5 36.05182380514744 37.0 35.0 37.0 35.0 37.0 6 36.11687922011977 37.0 35.0 37.0 35.0 37.0 7 36.06187655533314 37.0 35.0 37.0 35.0 37.0 8 36.05445292140348 37.0 35.0 37.0 35.0 37.0 9 37.715357773833055 39.0 37.0 39.0 35.0 39.0 10-11 37.64749541530804 39.0 37.0 39.0 35.0 39.0 12-13 37.54017900232067 39.0 37.0 39.0 35.0 39.0 14-15 38.637172514509444 40.0 38.0 41.0 34.0 41.0 16-17 38.62534673700025 40.0 38.0 41.0 34.5 41.0 18-19 38.724071835933074 40.0 38.0 41.0 35.0 41.0 20-21 38.69704575879275 40.0 38.0 41.0 34.5 41.0 22-23 38.6230737999018 40.0 38.0 41.0 34.0 41.0 24-25 38.608982953758606 40.0 38.0 41.0 34.0 41.0 26-27 38.4444685744021 40.0 38.0 41.0 34.0 41.0 28-29 38.272919787824236 40.0 38.0 41.0 34.0 41.0 30-31 38.1756152090074 40.0 37.0 41.0 34.0 41.0 32-33 37.958850029165774 40.0 36.5 41.0 33.5 41.0 34-35 37.75440424183673 40.0 36.0 41.0 33.0 41.0 36-37 37.549469036018685 40.0 36.0 41.0 33.0 41.0 38-39 37.374492221760605 40.0 35.5 41.0 33.0 41.0 40-41 37.13018741633199 40.0 35.0 41.0 33.0 41.0 42-43 36.81591097934056 40.0 35.0 41.0 31.5 41.0 44-45 36.33443806574259 39.0 35.0 41.0 30.5 41.0 46-47 35.67508854645331 39.0 35.0 41.0 28.0 41.0 48-49 35.10157400762927 39.0 35.0 41.0 24.5 41.0 50-51 34.7410451628877 39.0 35.0 40.5 23.0 41.0 52-53 34.47687930405006 39.0 35.0 40.0 22.0 41.0 54-55 34.237914563163834 38.0 34.0 40.0 20.0 41.0 56-57 33.966124162270816 38.0 34.0 40.0 19.0 41.0 58-59 33.716543813708334 38.0 34.0 40.0 17.5 41.0 60-61 33.378893840775845 38.0 33.5 40.0 13.5 41.0 62-63 33.01966591549059 37.0 33.0 40.0 9.0 41.0 64-65 32.602835060199 36.5 33.0 39.5 7.5 41.0 66-67 32.177437545374815 36.0 32.0 39.0 3.5 40.5 68-69 31.737785520346797 35.5 31.5 39.0 2.0 40.0 70-71 31.333153932343798 35.0 31.0 38.0 2.0 40.0 72-73 30.850789784005407 35.0 31.0 37.5 2.0 39.5 74-75 30.38352836633892 35.0 30.0 37.0 2.0 39.0 76-77 29.158623522302378 33.5 28.5 35.5 2.0 38.5 78-79 29.697631172548082 34.0 30.0 36.0 2.0 38.5 80-81 29.4910294259588 34.0 30.0 36.0 2.0 37.5 82-83 29.12167688517917 34.0 29.0 35.0 2.0 37.0 84-85 28.743834795818593 34.0 29.0 35.0 2.0 36.5 86-87 28.385701111656658 34.0 29.0 35.0 2.0 36.0 88-89 27.955027004570006 34.0 28.5 35.0 2.0 36.0 90-91 27.42146905280474 33.5 26.5 35.0 2.0 35.0 92-93 26.99881920576769 33.0 26.0 35.0 2.0 35.0 94-95 26.54679270897592 33.0 25.0 35.0 2.0 35.0 96-97 25.99622366162665 33.0 24.5 35.0 2.0 35.0 98-99 25.17562517572904 32.0 19.5 34.0 2.0 35.0 100-101 22.642498415815822 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29839848393531554 1101 2 0.20754752877350313 1101 3 0.2721963585705254 1101 4 0.13167482610123926 1101 5 0.09483796655067067 1101 6 0.18324817654625747 1101 7 0.18422660254707068 1101 8 0.12936017065283067 1101 9 0.14243259422776333 1101 10-11 0.22979913735319002 1101 12-13 0.2544445672003164 1101 14-15 0.39463049709232934 1101 16-17 0.3309514511677847 1101 18-19 0.3688495605377895 1101 20-21 0.21167241084854993 1101 22-23 0.24774985502526192 1101 24-25 0.3648708146777295 1101 26-27 0.2666541417256809 1101 28-29 0.17516077647857742 1101 30-31 0.32511929354075875 1101 32-33 0.1945261888351908 1101 34-35 0.19872292153134907 1101 36-37 0.24203967009326988 1101 38-39 0.13414345130140504 1101 40-41 0.3705679200604379 1101 42-43 0.5031957196226884 1101 44-45 0.523068255079636 1101 46-47 0.47283147971372586 1101 48-49 0.42011186876455753 1101 50-51 0.5065823505899729 1101 52-53 0.6178713844828607 1101 54-55 0.4621450424463447 1101 56-57 0.3998485243213352 1101 58-59 0.39179209498781375 1101 60-61 0.6179840387263269 1101 62-63 0.4431274395976459 1101 64-65 0.4919921856850067 1101 66-67 0.46296975099965465 1101 68-69 0.3517498563656467 1101 70-71 0.3811692201867203 1101 72-73 0.26374829127796673 1101 74-75 0.262583415466473 1101 76-77 0.4128442231173821 1101 78-79 0.36261103691409247 1101 80-81 0.43041525717546136 1101 82-83 0.5483855528578054 1101 84-85 0.4782556365673294 1101 86-87 0.5356180602990364 1101 88-89 0.525763322395278 1101 90-91 0.4412969358092802 1101 92-93 0.2731083017494882 1101 94-95 0.22689903039148973 1101 96-97 0.34855909383438544 1101 98-99 0.27612972727499496 1101 100-101 0.45898086345227185 1102 1 0.315953152142626 1102 2 0.19047729476238828 1102 3 0.15175246857671 1102 4 0.09886367618994285 1102 5 0.012236184241849912 1102 6 0.03513401879709477 1102 7 -0.04340122928396539 1102 8 0.09511105643884576 1102 9 0.022259680618105904 1102 10-11 0.038564584285275316 1102 12-13 0.1067758588373735 1102 14-15 0.13378505077377412 1102 16-17 0.2128888300592351 1102 18-19 0.11715467994634565 1102 20-21 0.06844605353806799 1102 22-23 0.23648580132080355 1102 24-25 0.17811248471029728 1102 26-27 0.07770691054192014 1102 28-29 0.07348583565880062 1102 30-31 0.21777813427311088 1102 32-33 0.293703545435001 1102 34-35 0.2656080735593278 1102 36-37 0.30186473694263327 1102 38-39 0.3820568053243747 1102 40-41 0.2748033320723522 1102 42-43 0.27977377360322464 1102 44-45 0.3606965214954343 1102 46-47 0.17363201041841592 1102 48-49 0.3666950099432498 1102 50-51 0.5248898729966456 1102 52-53 0.4721851495718994 1102 54-55 0.49858944882912226 1102 56-57 0.4125818579955265 1102 58-59 0.41079111845028393 1102 60-61 0.3366210786416559 1102 62-63 0.298870221655946 1102 64-65 0.3966596260829789 1102 66-67 0.23628650194168443 1102 68-69 0.31137245784119116 1102 70-71 0.3294363773376858 1102 72-73 0.33041231635094803 1102 74-75 0.3936586548715546 1102 76-77 0.3586700364299915 1102 78-79 0.530353087847427 1102 80-81 0.6810089766586671 1102 82-83 0.6533333004305213 1102 84-85 0.5905312502265865 1102 86-87 0.5808679209762744 1102 88-89 0.38230561260587237 1102 90-91 0.5672581350587258 1102 92-93 0.5005463222925677 1102 94-95 0.5117482550411374 1102 96-97 0.38468970445154227 1102 98-99 0.2776910369600998 1102 100-101 0.16446468411702853 1103 1 0.4125093631546548 1103 2 0.3299250529372202 1103 3 0.2763718148089893 1103 4 0.2069720798223571 1103 5 0.18682300063689183 1103 6 0.2136462844592586 1103 7 0.2613131368762609 1103 8 0.1490928178611881 1103 9 0.3164141508738041 1103 10-11 0.34175838557444393 1103 12-13 0.3404501176218062 1103 14-15 0.4638822575815311 1103 16-17 0.42793565662326927 1103 18-19 0.2653458569797351 1103 20-21 0.2814522478751442 1103 22-23 0.2235278550196469 1103 24-25 0.26508401153511585 1103 26-27 0.041345933225329645 1103 28-29 0.12181451276052258 1103 30-31 0.19619909770300836 1103 32-33 0.25170451859651877 1103 34-35 0.14619896848521563 1103 36-37 0.1737183636094457 1103 38-39 0.15319914933150613 1103 40-41 0.24561532054750046 1103 42-43 0.22579542918059303 1103 44-45 0.20195979995630609 1103 46-47 0.2684476017780568 1103 48-49 0.10166977881738859 1103 50-51 0.16266056771369364 1103 52-53 0.3481296082918135 1103 54-55 -0.053960589714634466 1103 56-57 0.2963040914558732 1103 58-59 0.016696240669524798 1103 60-61 0.12773168555547443 1103 62-63 0.234351116527165 1103 64-65 0.11770670413818607 1103 66-67 0.13836349104555268 1103 68-69 0.047867791586387654 1103 70-71 0.07743743250719604 1103 72-73 0.0801567247726247 1103 74-75 0.16213300550492704 1103 76-77 0.04668634508620784 1103 78-79 0.19208515133254878 1103 80-81 0.2413484077418815 1103 82-83 0.4645365490404849 1103 84-85 0.27743908417680174 1103 86-87 0.35279754171664024 1103 88-89 0.3186920124003052 1103 90-91 0.397982019613341 1103 92-93 0.5077063034853992 1103 94-95 0.38017394759863876 1103 96-97 0.42076092019270916 1103 98-99 0.7641412663091209 1103 100-101 0.7201624627938585 1104 1 0.3158980022452056 1104 2 0.2267797147398909 1104 3 0.2117031645684122 1104 4 0.07584410900633998 1104 5 0.05510419950734047 1104 6 0.08618903633476549 1104 7 0.15468341477859582 1104 8 0.21097547593102206 1104 9 0.22033605219605334 1104 10-11 0.23043383490847447 1104 12-13 0.22382324362924066 1104 14-15 0.18645182406357463 1104 16-17 0.32360638537458186 1104 18-19 0.18675936548162753 1104 20-21 0.2623972127889118 1104 22-23 0.2655525547566455 1104 24-25 0.3204667786813147 1104 26-27 0.30636665773479166 1104 28-29 0.2308257355065848 1104 30-31 0.3489976639547834 1104 32-33 0.29350500580428474 1104 34-35 0.3338284966693408 1104 36-37 0.32556402661194994 1104 38-39 0.19379297924509586 1104 40-41 0.07497595125128953 1104 42-43 0.15539420994922182 1104 44-45 0.11397380414969405 1104 46-47 0.11556192592877323 1104 48-49 0.17198691835029933 1104 50-51 0.2389390336152104 1104 52-53 0.2812741284164062 1104 54-55 0.14398801713777942 1104 56-57 0.27551368169233115 1104 58-59 0.13471762569697177 1104 60-61 0.2203375199290747 1104 62-63 0.21062624953972886 1104 64-65 0.1714798374173867 1104 66-67 0.20970700887954052 1104 68-69 0.3584566827645297 1104 70-71 0.07303347424708662 1104 72-73 0.24251992483079832 1104 74-75 0.1283200896512824 1104 76-77 -0.02683188278643911 1104 78-79 0.17056756579849974 1104 80-81 0.08069407074438928 1104 82-83 0.05309974061494316 1104 84-85 0.14776167752799196 1104 86-87 0.11655404085009025 1104 88-89 0.08955892711470526 1104 90-91 0.30877194459304036 1104 92-93 0.15537828055512293 1104 94-95 0.07189611191612144 1104 96-97 -0.11069700662917015 1104 98-99 0.18997236761682856 1104 100-101 -0.04379097977743385 1105 1 0.21346414193168783 1105 2 0.09393512380074043 1105 3 0.14657619507181607 1105 4 0.02835578071572087 1105 5 -0.06364834689563281 1105 6 0.0342015061953731 1105 7 0.06672564666323666 1105 8 0.029888654443055884 1105 9 0.1415595278607924 1105 10-11 0.018643237731062356 1105 12-13 0.06613661316324482 1105 14-15 0.0724109580527923 1105 16-17 0.016356149936676445 1105 18-19 -4.722185980980953E-4 1105 20-21 -0.18578640874713415 1105 22-23 -0.060697603871737726 1105 24-25 -0.1256233656538157 1105 26-27 -0.16054930595767303 1105 28-29 -0.08574558715356773 1105 30-31 -0.031760128873209226 1105 32-33 0.008124197500841035 1105 34-35 -0.11069164412587185 1105 36-37 -0.0547320741354369 1105 38-39 0.004537213520848127 1105 40-41 0.19851657013574453 1105 42-43 0.28127084048200857 1105 44-45 0.4166981213797385 1105 46-47 0.5023129494992773 1105 48-49 0.7816488061282385 1105 50-51 0.646337210891609 1105 52-53 0.6436744430015295 1105 54-55 0.6687703622997887 1105 56-57 0.94182343449922 1105 58-59 0.8980010829274292 1105 60-61 0.9042546057086227 1105 62-63 0.7600327289247204 1105 64-65 0.9187823200350564 1105 66-67 0.8451464261957469 1105 68-69 0.9139198442448908 1105 70-71 0.7981079460980638 1105 72-73 0.8342689112789223 1105 74-75 0.80736018756674 1105 76-77 0.8068850807657668 1105 78-79 0.7672594961222323 1105 80-81 0.7750756204267972 1105 82-83 0.857931310668576 1105 84-85 0.9409966779621328 1105 86-87 0.8290062853582079 1105 88-89 0.8053469595216498 1105 90-91 0.7128314118688373 1105 92-93 0.5602116873584428 1105 94-95 0.5487342809104554 1105 96-97 0.48850667889256627 1105 98-99 0.6685475173954138 1105 100-101 0.5969520522621607 1106 1 0.16783818163103348 1106 2 0.0835560905380035 1106 3 0.07854148928717564 1106 4 -9.554501301778373E-4 1106 5 0.12257341507438468 1106 6 0.09448373077076155 1106 7 0.12143812745722471 1106 8 -0.0669680479364132 1106 9 0.12840081352168653 1106 10-11 -0.05274019215142545 1106 12-13 -0.1682507150208039 1106 14-15 0.07447819986168724 1106 16-17 0.17543254834205158 1106 18-19 0.18675461479752897 1106 20-21 0.24409282695735612 1106 22-23 0.2562554659758618 1106 24-25 0.19967588479259035 1106 26-27 0.21020331021274785 1106 28-29 0.12932736974191528 1106 30-31 0.2919942054567599 1106 32-33 0.11320704289762062 1106 34-35 -0.1357386209956033 1106 36-37 0.15542777699200627 1106 38-39 0.08929218113016901 1106 40-41 0.0723298202084166 1106 42-43 -0.13375617770660142 1106 44-45 -0.09833901889683005 1106 46-47 0.10041674502272002 1106 48-49 0.0293998858176181 1106 50-51 0.10406711205850883 1106 52-53 0.2737062886474746 1106 54-55 0.33984922115116234 1106 56-57 0.5411671857969225 1106 58-59 0.6945334891813175 1106 60-61 0.7943579288679601 1106 62-63 0.6734758998893824 1106 64-65 0.3736689526499788 1106 66-67 0.2700074421419245 1106 68-69 0.633074727763006 1106 70-71 0.6748643878986513 1106 72-73 0.7003718555088163 1106 74-75 0.42625449220524914 1106 76-77 0.3691281354561866 1106 78-79 0.41076215381917436 1106 80-81 0.1055306472114026 1106 82-83 0.01035308509655053 1106 84-85 -0.16912320389493374 1106 86-87 0.12810295389356696 1106 88-89 0.3305658821162254 1106 90-91 0.5741309062835249 1106 92-93 0.40982017018723127 1106 94-95 0.1805822627218383 1106 96-97 0.47267274861558306 1106 98-99 -0.035447592857483556 1106 100-101 -0.6039441418327698 1107 1 0.2564419715502311 1107 2 0.20497298164997346 1107 3 0.13641230498551948 1107 4 0.12814468453700556 1107 5 0.06828570826407088 1107 6 -0.0012622292138431135 1107 7 0.04271794661843842 1107 8 0.09582292936254788 1107 9 0.1053150112900525 1107 10-11 0.18149052158462808 1107 12-13 0.16674591829970353 1107 14-15 0.13643712637188088 1107 16-17 0.183668889242405 1107 18-19 0.3344015463096568 1107 20-21 0.26322879271342003 1107 22-23 0.2095968953717815 1107 24-25 0.10628660045245653 1107 26-27 0.22175823518389137 1107 28-29 0.3532536394610304 1107 30-31 0.24436170257103385 1107 32-33 0.1051270121165544 1107 34-35 0.17629244864657068 1107 36-37 0.2066674364764296 1107 38-39 0.26601279482773776 1107 40-41 0.23091846006990124 1107 42-43 0.15613296234725738 1107 44-45 0.16335418041060024 1107 46-47 0.11631228176762676 1107 48-49 0.24555273306656034 1107 50-51 0.3944792898191807 1107 52-53 0.39064429364939457 1107 54-55 0.22848427309421737 1107 56-57 0.11643050822862477 1107 58-59 0.11117811211321538 1107 60-61 -0.04535806766916295 1107 62-63 -0.06324976382742875 1107 64-65 0.03575052248453403 1107 66-67 0.103434962342007 1107 68-69 0.1561004107179933 1107 70-71 0.13335202737537344 1107 72-73 0.16487743457768644 1107 74-75 0.15820668535646476 1107 76-77 0.08139727743386871 1107 78-79 0.08433852934822283 1107 80-81 0.28370013547611705 1107 82-83 0.23434548601998273 1107 84-85 0.23952555524319408 1107 86-87 0.061244306336817544 1107 88-89 0.10330900447097946 1107 90-91 0.34332699131622846 1107 92-93 0.1895852240541025 1107 94-95 0.19627339392615184 1107 96-97 0.5062734123323942 1107 98-99 0.47959425015906376 1107 100-101 0.7211929301179794 1108 1 0.07065713200433521 1108 2 0.03252952048969604 1108 3 -0.013918436053188543 1108 4 0.1037190118812461 1108 5 0.047625567028710236 1108 6 -0.0054970553513271625 1108 7 -0.11979210969692389 1108 8 -0.1174044774489289 1108 9 -0.028109946249948337 1108 10-11 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62-63 -0.7835331807008572 2316 64-65 -0.6582303218898211 2316 66-67 -0.5790670717503872 2316 68-69 -0.6795286009642254 2316 70-71 -0.50825031007302 2316 72-73 -0.360595178000537 2316 74-75 -0.27937430165547994 2316 76-77 -0.16547310818986816 2316 78-79 -0.4913654926300204 2316 80-81 -0.5733822728892761 2316 82-83 -0.7708383105506904 2316 84-85 -0.6670342671629577 2316 86-87 -0.6909800113633047 2316 88-89 -0.7419223044800347 2316 90-91 -0.3639790416511346 2316 92-93 -0.08182485399477812 2316 94-95 -0.24120714407873223 2316 96-97 -0.20179163844457548 2316 98-99 -0.34787202408681495 2316 100-101 -0.0795909488208757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 7.0 5 20.0 6 63.0 7 135.0 8 323.0 9 653.0 10 1086.0 11 1880.0 12 3088.0 13 4416.0 14 5726.0 15 8449.0 16 12600.0 17 19354.0 18 17340.0 19 8787.0 20 6173.0 21 6100.0 22 6103.0 23 6350.0 24 7004.0 25 8041.0 26 9606.0 27 11385.0 28 13987.0 29 16969.0 30 20656.0 31 26097.0 32 32765.0 33 43261.0 34 60107.0 35 88625.0 36 131316.0 37 199392.0 38 161234.0 39 14061.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.872556323913656 20.292244246933272 15.701123854895746 19.134075574257324 2 15.587847733672412 22.41054080480753 40.119411816545174 21.882199644974882 3 19.30553981862665 27.908289374845253 31.4890701783099 21.297100628218203 4 11.90739971379772 18.484701606845356 37.87406680011583 31.7338318792411 5 11.787384660911211 40.161561297485385 33.34777968646866 14.703274355134734 6 28.354903417221657 38.29539684338189 17.577310286076383 15.772389453320073 7 25.858816666876493 36.920303995501335 20.27787219935122 16.943007138270954 8 27.184075906552017 34.26884130041587 19.290012715438557 19.257070077593553 9 27.735707721166797 16.48369863990969 20.791420646011424 34.98917299291209 10-11 24.52584633203661 28.50938760265723 25.997459010545843 20.96730705476032 12-13 26.597166631169994 26.843090371728838 27.47557811488542 19.08416488221575 14-15 23.105221303185573 26.84011909707797 28.425142576575897 21.629517023160563 16-17 19.519876790337946 30.536408958718887 28.735579727478356 21.20813452346481 18-19 19.394978031247245 27.302732350509668 34.055396088009296 19.246893530233788 20-21 21.02558614814535 25.812392726601285 34.38067840851389 18.781342716739477 22-23 20.38512724180638 26.447295878837412 34.23765337708929 18.92992350226692 24-25 19.900164765904947 26.643764153422712 34.765553487020426 18.690517593651922 26-27 19.836669861298425 26.64034374027906 34.82054195432778 18.70244444409473 28-29 19.202759235702203 26.927412691959663 34.998282034805186 18.871546037532948 30-31 19.321381660392877 26.755297050270045 34.90209531962752 19.02122596970956 32-33 19.24238227728049 27.067098068344432 34.52509095944908 19.165428694925996 34-35 19.692878095453917 26.698749019064767 34.652874822172706 18.955498063308614 36-37 19.947134410158924 26.5426911407385 34.498495549596505 19.01167889950607 38-39 19.74480996084652 26.970473324856375 34.4006118517959 18.884104862501204 40-41 19.423476201816904 27.386926238064714 34.33701127091855 18.85258628919983 42-43 19.540032695168964 27.55208038226906 33.58862006660749 19.31926685595449 44-45 19.578523078730807 27.722383788025667 33.09465657824611 19.60443655499742 46-47 20.02492729538845 28.139936968354085 32.28866353606694 19.54647220019052 48-49 20.215449048020716 28.60700901830939 31.455078411871103 19.722463521798794 50-51 20.330118803321962 28.88497563923405 31.011087190979175 19.773818366464816 52-53 20.383257166597424 29.552955814900145 30.379459321087904 19.684327697414528 54-55 19.801242170861443 29.510895223307486 30.49387832181038 20.19398428402069 56-57 20.173536360698805 29.66788785234984 30.164125677212507 19.99445010973885 58-59 20.20737075784853 29.182875703440725 30.55587029413369 20.053883244577055 60-61 20.172382319245635 29.063170130889283 31.038154708698958 19.726292841166128 62-63 20.137498793502118 29.114524975555305 31.134464713608878 19.613511517333706 64-65 19.99980066556718 29.159322766510137 31.047596866043065 19.79327970187962 66-67 19.853657052452235 29.390078600714247 30.638436714464966 20.117827632368552 68-69 19.955212700331103 29.498506040882443 30.565994385063767 19.980286873722687 70-71 20.023091320349316 29.837584402395372 30.239872761684143 19.89945151557117 72-73 20.10550037139152 29.54713315120461 30.049193639762812 20.298172837641054 74-75 19.94099700788525 30.21317243897219 29.622932692105934 20.222897861036625 76-77 19.971316824245783 29.79042607210451 29.974915335322482 20.26334176832723 78-79 19.935331713478785 29.92361294708615 29.617844418426053 20.52321092100901 80-81 19.88544564884407 30.312682286093175 29.570423805986746 20.23144825907601 82-83 19.86516443706782 30.255224458407344 29.486151867313076 20.39345923721176 84-85 19.698606179475572 30.169387145964816 29.29394133164913 20.838065342910483 86-87 19.693822311188327 29.89156206854587 29.436240258841007 20.9783753614248 88-89 19.772454499292888 29.680897046912836 29.42973566155951 21.11691279223477 90-91 19.57322389979738 29.61342204696814 29.57213264234352 21.241221410890958 92-93 19.610714913327545 29.933772750043936 29.51206909666031 20.94344323996821 94-95 19.26357467487505 29.876979181092185 29.45145262345096 21.407993520581805 96-97 19.43972336577239 29.716095311234486 29.36909602883845 21.475085294154674 98-99 19.335145209779515 30.10689231715077 28.834119931113023 21.72384254195669 100-101 19.057247925337872 30.237941460870644 29.293751800261596 21.411058813529888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2026.0 1 1371.0 2 829.0 3 994.0 4 898.5 5 900.0 6 1636.5 7 3333.0 8 4943.5 9 5604.5 10 5006.5 11 4148.0 12 4169.5 13 4384.5 14 4458.0 15 4489.0 16 4458.5 17 4447.5 18 4500.0 19 4677.5 20 4998.0 21 5354.0 22 5870.5 23 6483.0 24 7153.0 25 8248.0 26 9659.0 27 11090.0 28 11996.0 29 13016.0 30 14855.0 31 16286.0 32 17987.5 33 20361.5 34 23530.5 35 25550.0 36 27030.0 37 28962.0 38 29806.5 39 29693.0 40 29189.0 41 30051.0 42 30799.5 43 31596.0 44 32774.0 45 33109.5 46 31920.0 47 31655.5 48 37219.5 49 36456.0 50 29717.0 51 26289.5 52 23600.5 53 22401.0 54 20854.5 55 18903.5 56 16759.0 57 14744.5 58 13641.5 59 12288.5 60 10658.5 61 9071.0 62 7241.5 63 5279.5 64 3510.0 65 2557.5 66 1855.0 67 1333.0 68 906.5 69 556.0 70 410.0 71 320.0 72 300.0 73 276.0 74 207.0 75 147.5 76 121.5 77 81.0 78 49.0 79 42.5 80 27.5 81 16.0 82 13.5 83 12.0 84 10.0 85 5.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02119239759455796 2 0.0 3 0.0 4 0.0 5 0.02318574192275896 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003252298640748994 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.819335859634988E-4 24-25 1.5736928906849972E-4 26-27 0.0011015850234794978 28-29 9.96672164100498E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0011540414531689977 42-43 0.013219020281753974 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.0982571875799962E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0011540414531689977 84-85 5.2456429689499904E-5 86-87 0.0 88-89 0.0 90-91 0.015002538891196971 92-93 0.0015212364609954972 94-95 0.0 96-97 0.0 98-99 0.0017310621797534968 100-101 0.019461335414804465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 953172.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.34589411187735 #Duplication Level Percentage of deduplicated Percentage of total 1 74.46291762619644 35.25513413191342 2 13.365991960238945 12.656496800993544 3 4.797205914211633 6.813840097414073 4 2.271569257941643 4.301979102172034 5 1.2950116755048948 3.0656742831049812 6 0.7889545225009186 2.2412254368849154 7 0.5362516752223444 1.7772520522675774 8 0.3658583535479912 1.3857512693623182 9 0.2644807427053911 1.1269869514884114 >10 1.636064730054359 14.46964305566516 >50 0.13711991215758187 4.4491374990842205 >100 0.07300892507944225 6.373872851354395 >500 0.002671058226466239 0.8006202272939944 >1k 0.002225881855388533 1.7766033686615976 >5k 2.2258818553885323E-4 0.8664867064382284 >10k+ 4.4517637107770647E-4 2.639296165901118 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13299 1.3952361168813183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11745 1.2322015334063527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8222 0.8625935298141364 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2703 0.2835794589014365 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2432 0.2551480740097275 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2291 0.24035536083728856 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2290 0.24025044797790956 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1423 0.14929099889631672 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1381 0.14488465880239873 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1219 0.12788877558300077 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1046 0.10973885091043381 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1042 0.10931919947291781 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1031 0.1081651580197488 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0491285937899981E-4 5.2456429689499904E-5 18-19 0.0 0.0 0.0 2.0982571875799962E-4 1.0491285937899981E-4 20-21 0.0 0.0 0.0 2.622821484474995E-4 1.0491285937899981E-4 22-23 0.0 0.0 0.0 3.671950078264993E-4 1.0491285937899981E-4 24-25 0.0 0.0 0.0 6.294771562739988E-4 1.0491285937899981E-4 26-27 0.0 0.0 0.0 0.0012064978828584978 1.0491285937899981E-4 28-29 0.0 0.0 0.0 0.003567037218885993 1.0491285937899981E-4 30-31 0.0 0.0 0.0 0.007973377312803984 1.0491285937899981E-4 32-33 0.0 0.0 0.0 0.013061650992685477 1.0491285937899981E-4 34-35 0.0 0.0 0.0 0.019041683977288462 1.0491285937899981E-4 36-37 0.0 0.0 0.0 0.02570365054785495 1.0491285937899981E-4 38-39 0.0 0.0 0.0 0.03283772498562694 1.0491285937899981E-4 40-41 0.0 0.0 0.0 0.040181625142156924 1.0491285937899981E-4 42-43 0.0 0.0 0.0 0.055761184759938395 1.0491285937899981E-4 44-45 0.0 0.0 0.0 0.07475041230753736 1.0491285937899981E-4 46-47 0.0 0.0 0.0 0.09358227056606783 1.0491285937899981E-4 48-49 0.0 0.0 0.0 0.11744994607479028 1.0491285937899981E-4 50-51 0.0 0.0 0.0 0.14787467529470022 1.0491285937899981E-4 52-53 0.0 0.0 0.0 0.20269164432022763 1.0491285937899981E-4 54-55 0.0 0.0 0.0 0.2528399911033895 1.0491285937899981E-4 56-57 0.0 0.0 0.0 0.33183937421577636 1.0491285937899981E-4 58-59 0.0 0.0 0.0 0.4406340093917992 1.0491285937899981E-4 60-61 0.0 0.0 0.0 0.5498482960053379 1.0491285937899981E-4 62-63 0.0 0.0 0.0 0.6999785977766867 1.0491285937899981E-4 64-65 0.0 0.0 0.0 0.8043668928587915 1.0491285937899981E-4 66-67 0.0 0.0 0.0 0.9758469615137666 1.0491285937899981E-4 68-69 0.0 0.0 0.0 1.1765977179354827 1.0491285937899981E-4 70-71 0.0 0.0 0.0 1.343041969340266 1.0491285937899981E-4 72-73 0.0 0.0 0.0 1.6163399680225605 1.0491285937899981E-4 74-75 0.0 0.0 0.0 1.884811975173421 1.0491285937899981E-4 76-77 0.0 0.0 0.0 2.0849857108685526 1.0491285937899981E-4 78-79 0.0 0.0 0.0 2.3150071550570095 1.0491285937899981E-4 80-81 0.0 0.0 0.0 2.6695601633283395 1.0491285937899981E-4 82-83 0.0 0.0 0.0 3.003602707591075 1.0491285937899981E-4 84-85 0.0 0.0 0.0 3.418113414997503 1.0491285937899981E-4 86-87 0.0 0.0 0.0 3.943831753345671 1.0491285937899981E-4 88-89 0.0 0.0 0.0 4.614329837636859 1.0491285937899981E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 70 2.0458674E-4 33.948875 1 GGTATCA 9290 0.0 31.873207 1 CGCGTAA 30 0.003952208 31.66567 10-11 GGGTCGC 60 0.00395224 31.664007 6 GTATCAA 13110 0.0 28.35029 1 CATGGGT 715 0.0 23.92035 4 GAGTACT 9345 0.0 23.660301 12-13 GTATAGA 350 0.0 23.085234 1 TATCAAC 16200 0.0 22.903778 2 ATCAACG 16340 0.0 22.707539 3 AGTACTT 9455 0.0 22.706848 12-13 TCAACGC 16400 0.0 22.65343 4 CCGTCCA 105 0.0021963506 22.618334 9 GTACTTT 9775 0.0 22.618305 14-15 GTACATG 8820 0.0 22.363148 1 CAACGCA 16610 0.0 22.28122 5 AACGCAG 16895 0.0 21.98395 6 GCGGTAT 130 2.9291236E-4 21.936195 1 TACATGG 8945 0.0 21.935158 2 ACATGGG 8810 0.0 21.354548 3 >>END_MODULE