FastQCFastQC Report
Thu 26 May 2016
SRR937786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914282
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152391.6667723962628598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121351.3272710170385067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82680.9043161737844561No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25950.2838292780564421No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.24521974620521897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13880.15181311674078676No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.13814118619856894No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12610.13792243530989345No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.13464117197976117No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.1252348837667153No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11240.1229379994356227No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11220.12271924854694721No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.11331296033390136No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10340.11309420944522587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC407.5379085E-659.38348
TATCACG405.373658E-447.5249522
CAACGTA405.383837E-447.5067189
GGTATCA74700.038.1173931
GTATCAA116900.033.913181
GACCGGT856.400865E-427.9451277
GTACATG74950.027.5889151
ATCAACG143600.027.4345573
TCAACGC144050.027.3878544
TATCAAC144100.027.3408642
TATAACG700.00836920627.1571182
CAACGCA145600.027.0962925
AACGCAG147850.026.7335726
TACATGG76400.026.6239262
GTATAGG2155.638867E-1126.5313761
ACATGGG76950.025.5675773
GAGTACT91900.025.53469712-13
ACGCAGA162250.024.2730757
CGCAGAG162150.024.1708558
AGTACTT93400.024.10742212-13
Produced by FastQC (version 0.11.5)