##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937786_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 914282 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84576749843046 33.0 31.0 34.0 30.0 34.0 2 31.923875784495376 33.0 31.0 34.0 30.0 34.0 3 31.91327183516683 33.0 31.0 34.0 30.0 34.0 4 35.54166110674824 37.0 35.0 37.0 33.0 37.0 5 35.54817988323077 37.0 35.0 37.0 33.0 37.0 6 35.65984783688184 37.0 35.0 37.0 33.0 37.0 7 35.61134310858138 37.0 35.0 37.0 33.0 37.0 8 35.61307233435636 37.0 35.0 37.0 33.0 37.0 9 37.21441633981638 39.0 37.0 39.0 34.0 39.0 10-11 37.12295714013838 39.0 37.0 39.0 34.0 39.0 12-13 36.994180132606786 39.0 37.0 39.0 33.0 39.0 14-15 38.03323372876203 40.0 38.0 41.0 33.0 41.0 16-17 38.01473670049285 40.0 38.0 41.0 33.0 41.0 18-19 38.1108443565552 40.0 38.0 41.0 33.5 41.0 20-21 37.99071129038961 40.0 38.0 41.0 33.0 41.0 22-23 37.892291984311186 40.0 38.0 41.0 33.0 41.0 24-25 37.75148477165689 40.0 37.0 41.0 33.0 41.0 26-27 37.79528690272804 40.0 37.0 41.0 33.0 41.0 28-29 37.71489704489424 40.0 37.0 41.0 33.0 41.0 30-31 37.394107616687194 39.5 36.5 41.0 33.0 41.0 32-33 37.027040891103624 39.0 35.5 41.0 32.0 41.0 34-35 36.707699593779594 39.0 35.0 41.0 31.0 41.0 36-37 36.63415773251579 39.0 35.0 41.0 31.0 41.0 38-39 36.54273025171665 39.0 35.0 41.0 31.0 41.0 40-41 36.29314861279124 39.0 35.0 41.0 30.0 41.0 42-43 35.93293972756764 39.0 35.0 41.0 30.0 41.0 44-45 35.49133473042234 39.0 35.0 41.0 27.5 41.0 46-47 34.83217869322594 39.0 35.0 40.5 23.5 41.0 48-49 34.248120929866275 38.5 34.5 40.0 19.0 41.0 50-51 33.25309751258365 37.5 33.5 39.5 14.0 40.5 52-53 33.33245103808234 38.0 33.5 39.5 11.0 40.5 54-55 33.62262354503315 38.0 34.0 40.0 8.5 41.0 56-57 33.39053814906123 38.0 33.5 40.0 7.0 41.0 58-59 33.18338926064387 38.0 33.0 40.0 3.5 41.0 60-61 32.88639664786138 38.0 33.0 40.0 2.0 41.0 62-63 32.546869565407604 37.0 33.0 40.0 2.0 41.0 64-65 32.186607086216284 36.5 32.0 40.0 2.0 41.0 66-67 31.77630534124045 36.0 31.5 39.0 2.0 41.0 68-69 31.3201703631921 35.5 31.0 39.0 2.0 40.5 70-71 30.880698734088607 35.0 31.0 38.5 2.0 40.0 72-73 30.422720232925947 35.0 30.0 38.0 2.0 40.0 74-75 29.946326735077363 35.0 29.5 37.0 2.0 39.5 76-77 29.499333903543985 35.0 29.0 37.0 2.0 39.0 78-79 28.987591355839882 34.0 29.0 36.0 2.0 39.0 80-81 28.47574763584977 34.0 28.0 36.0 2.0 37.5 82-83 27.989396597548676 34.0 27.0 35.0 2.0 37.0 84-85 27.53160020650084 34.0 26.0 35.0 2.0 36.5 86-87 27.046578079848448 33.5 25.5 35.0 2.0 36.0 88-89 26.572109589820208 33.0 25.0 35.0 2.0 36.0 90-91 26.08149564357605 33.0 23.5 35.0 2.0 35.0 92-93 25.57692210937107 32.5 20.5 35.0 2.0 35.0 94-95 25.00664619887518 32.0 18.5 35.0 2.0 35.0 96-97 24.30516952100118 32.0 4.5 34.0 2.0 35.0 98-99 23.48803596702112 31.5 2.0 34.0 2.0 35.0 100-101 21.14170627880676 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16354002964905945 1101 2 0.2787878925949663 1101 3 0.29035003570445284 1101 4 0.23296057246561475 1101 5 0.23503561714070287 1101 6 0.05938927642151981 1101 7 0.15260816734672744 1101 8 0.17719389502619975 1101 9 0.13188376461400964 1101 10-11 0.1495722545311864 1101 12-13 0.29493255561934006 1101 14-15 0.4783676092103377 1101 16-17 0.4379680293806345 1101 18-19 0.34677853892640087 1101 20-21 0.31250831484808117 1101 22-23 0.24780296930416057 1101 24-25 0.2982100275189623 1101 26-27 0.263166909347774 1101 28-29 0.21604709032249758 1101 30-31 0.3442487413412394 1101 32-33 0.3408444991215589 1101 34-35 0.47536503203115643 1101 36-37 0.6387582354318297 1101 38-39 0.6499811018751629 1101 40-41 0.7888602070306447 1101 42-43 0.7589043221046765 1101 44-45 1.0245119850824054 1101 46-47 1.190991062761249 1101 48-49 1.571852555400163 1101 50-51 1.4144147540969314 1101 52-53 1.2806424044567066 1101 54-55 1.6404925775764028 1101 56-57 1.5184826432923089 1101 58-59 1.3556650717661114 1101 60-61 1.4380388012165923 1101 62-63 1.527002333701894 1101 64-65 1.5362488810375048 1101 66-67 1.473790486863038 1101 68-69 1.6604177442468817 1101 70-71 1.6865418656529663 1101 72-73 1.606484143540225 1101 74-75 1.6938659340570084 1101 76-77 1.66102691737537 1101 78-79 1.629514526326897 1101 80-81 1.683110304676962 1101 82-83 1.7336092769522757 1101 84-85 1.895174884322845 1101 86-87 2.024685040401888 1101 88-89 1.942872561893246 1101 90-91 1.8448746726744112 1101 92-93 2.0065750337908597 1101 94-95 1.9809494105203953 1101 96-97 2.0108467708625604 1101 98-99 2.3675773569172556 1101 100-101 2.656334601776333 1102 1 0.09600093202014293 1102 2 0.18666993071652271 1102 3 0.1969895040205678 1102 4 0.0774874527709386 1102 5 0.0299386681814795 1102 6 0.09344863024135464 1102 7 0.008172862132980185 1102 8 0.061709479242388454 1102 9 0.1623239830781813 1102 10-11 0.08603833336253786 1102 12-13 0.062292067365078196 1102 14-15 0.22192006473883907 1102 16-17 0.2889095529269454 1102 18-19 0.15049966809662862 1102 20-21 0.190015936966482 1102 22-23 0.196838963250336 1102 24-25 0.16102039579340754 1102 26-27 0.21384631975772805 1102 28-29 0.34174487058614744 1102 30-31 0.45933834235898985 1102 32-33 0.398710634981704 1102 34-35 0.5064188584442562 1102 36-37 0.5963770637093333 1102 38-39 0.804379448039974 1102 40-41 0.899442613645995 1102 42-43 0.9363228367237824 1102 44-45 0.9709602972492846 1102 46-47 1.1869768384625985 1102 48-49 1.2955732458873186 1102 50-51 1.2277784113977148 1102 52-53 1.1036184304267422 1102 54-55 1.1723198570833162 1102 56-57 1.3001857206540635 1102 58-59 1.3646791863939995 1102 60-61 1.3031078389652677 1102 62-63 1.2445762517997636 1102 64-65 1.1945106541944845 1102 66-67 1.2510483338065086 1102 68-69 1.3315798157083627 1102 70-71 1.3674799894054637 1102 72-73 1.2277424511009265 1102 74-75 1.1243401504593677 1102 76-77 1.2485908005979347 1102 78-79 1.4118896113217438 1102 80-81 1.797986596050258 1102 82-83 1.8234488286181723 1102 84-85 1.8890250959874315 1102 86-87 1.8854404526543043 1102 88-89 1.8261647686884501 1102 90-91 1.8253233906231294 1102 92-93 1.728163998718152 1102 94-95 1.9361308059712528 1102 96-97 2.344179830018973 1102 98-99 2.5343430014523634 1102 100-101 2.384111191454121 1103 1 -0.01150428087912303 1103 2 0.16228074382975066 1103 3 0.04218744560637688 1103 4 -0.09415747078146097 1103 5 -0.01400136738015334 1103 6 -0.0473813722846117 1103 7 0.05647204540415629 1103 8 -0.057049281152458775 1103 9 -0.13806778920851315 1103 10-11 0.03825239159842653 1103 12-13 0.08484157133479897 1103 14-15 0.10295699831585381 1103 16-17 0.11954666579798356 1103 18-19 0.1176163033125519 1103 20-21 0.06910762951191174 1103 22-23 0.07516915150905135 1103 24-25 -0.01975050657060251 1103 26-27 -0.30490587807985037 1103 28-29 -0.1467084337233544 1103 30-31 0.07122499089813772 1103 32-33 0.24252239112203 1103 34-35 0.15717999126547255 1103 36-37 0.039904401094119635 1103 38-39 0.03996101443571831 1103 40-41 0.1309965652960372 1103 42-43 0.25630477111802463 1103 44-45 0.1842898416344596 1103 46-47 0.3752519631511859 1103 48-49 0.35652702071978837 1103 50-51 0.30192509322017 1103 52-53 0.2771448267655856 1103 54-55 0.2960875202681166 1103 56-57 0.5531630166823547 1103 58-59 0.6595407659643868 1103 60-61 0.5824793069474268 1103 62-63 0.5279830733923063 1103 64-65 0.6969483965757242 1103 66-67 0.8039570944489967 1103 68-69 0.9888001145647358 1103 70-71 0.9405534454710001 1103 72-73 0.9158070786696122 1103 74-75 1.0000812241432655 1103 76-77 0.7211710742937214 1103 78-79 0.9393455555127659 1103 80-81 1.0281534335910578 1103 82-83 0.70259190722523 1103 84-85 1.0182399912589197 1103 86-87 1.0154211859767415 1103 88-89 0.8912832909491399 1103 90-91 0.8600111491012079 1103 92-93 1.0304672705863247 1103 94-95 0.9813511593092414 1103 96-97 1.0839177858690583 1103 98-99 1.430628284484314 1103 100-101 1.7922202215329222 1104 1 0.26955349004173357 1104 2 0.2385406839165718 1104 3 0.3182657196355301 1104 4 0.15914487901566332 1104 5 0.1916880760068338 1104 6 0.23767989821551083 1104 7 0.2047878131741072 1104 8 0.21192156709747678 1104 9 0.37677477350887045 1104 10-11 0.41981519734390815 1104 12-13 0.29804205439774734 1104 14-15 0.385717589996311 1104 16-17 0.5186380170766114 1104 18-19 0.29488798482217504 1104 20-21 0.3252579649413363 1104 22-23 0.36618074167616044 1104 24-25 0.4426286182320709 1104 26-27 0.37382064444809515 1104 28-29 0.4317853142416581 1104 30-31 0.4836953069668297 1104 32-33 0.3841980336204287 1104 34-35 0.538026720679369 1104 36-37 0.3311174001682744 1104 38-39 0.453739294276275 1104 40-41 0.830200323442952 1104 42-43 0.8002620660610731 1104 44-45 0.8268853576193962 1104 46-47 0.9002991111453369 1104 48-49 0.9537155752805049 1104 50-51 0.9378840663054078 1104 52-53 1.0851356654466002 1104 54-55 1.1156983791537058 1104 56-57 1.2927460763622847 1104 58-59 1.4573700078310097 1104 60-61 1.2843833773975533 1104 62-63 1.1266762241361334 1104 64-65 1.287989601016914 1104 66-67 1.2363696786048806 1104 68-69 1.3372868808850278 1104 70-71 1.2884988755984281 1104 72-73 1.243713516669505 1104 74-75 1.2949916290229346 1104 76-77 1.458667999424538 1104 78-79 1.2785973652076201 1104 80-81 1.1389617679393957 1104 82-83 1.4203889714316809 1104 84-85 1.6489515856452641 1104 86-87 1.5629170682393045 1104 88-89 1.5602617815923807 1104 90-91 1.3350081836933896 1104 92-93 1.6549594693745284 1104 94-95 1.7073403118872328 1104 96-97 1.6697876375031377 1104 98-99 2.085208426521376 1104 100-101 2.1204713633144436 1105 1 0.16237182848085752 1105 2 0.055938669745739134 1105 3 0.21170435705877466 1105 4 0.1319507714558128 1105 5 0.15883524094032708 1105 6 0.169606086638332 1105 7 0.22060143533999366 1105 8 0.2923013101336167 1105 9 0.23734857007881516 1105 10-11 0.17922773418666793 1105 12-13 0.13900739969418652 1105 14-15 0.14741522825795528 1105 16-17 0.303862495275105 1105 18-19 0.3750381171859729 1105 20-21 0.2892135915533629 1105 22-23 0.3533865748877716 1105 24-25 0.2084367377456786 1105 26-27 0.13853073471160116 1105 28-29 0.15578078005618323 1105 30-31 0.16565751275001617 1105 32-33 0.3077091659862248 1105 34-35 0.3025257091918334 1105 36-37 0.2375157841893767 1105 38-39 0.26155916345322083 1105 40-41 0.32224799041843966 1105 42-43 0.2489053121056628 1105 44-45 0.22403678460720755 1105 46-47 0.3916939634641565 1105 48-49 0.548750882298485 1105 50-51 0.22730456698673862 1105 52-53 0.23273130654560958 1105 54-55 0.3731279602117823 1105 56-57 0.6390895138991795 1105 58-59 0.6372362033372454 1105 60-61 0.6543837175615081 1105 62-63 0.4501881568877195 1105 64-65 0.43618453097315424 1105 66-67 0.3209244339969821 1105 68-69 0.4788466126757527 1105 70-71 0.2738253029364053 1105 72-73 0.40257858963467186 1105 74-75 0.3493703395614105 1105 76-77 0.42065467700313164 1105 78-79 0.370822317634687 1105 80-81 0.40776132932798603 1105 82-83 0.6870049803479787 1105 84-85 0.6714099105578732 1105 86-87 0.5417328574497056 1105 88-89 0.72418629320698 1105 90-91 0.49411237191210944 1105 92-93 0.7533228805387111 1105 94-95 0.7744401540111383 1105 96-97 0.9208111308269196 1105 98-99 0.7405057798456767 1105 100-101 0.998751194192927 1106 1 -0.08936864359859698 1106 2 0.1602059096062014 1106 3 0.07651133203523841 1106 4 -0.08342981680613093 1106 5 -0.008144366717246498 1106 6 0.023665677986059563 1106 7 -0.09271374238196017 1106 8 -0.03641509485397165 1106 9 0.09206256886061226 1106 10-11 -0.14737317292271968 1106 12-13 -0.2845049560215642 1106 14-15 -0.12852070022034923 1106 16-17 0.018138141754143078 1106 18-19 -0.1438994271752918 1106 20-21 -0.015088382240143972 1106 22-23 -0.2787848496565388 1106 24-25 -0.3136419622991298 1106 26-27 -0.17034957416871066 1106 28-29 0.11931895969605932 1106 30-31 0.21310079324414488 1106 32-33 0.23316460839082254 1106 34-35 0.08294558706933941 1106 36-37 0.08791698628548517 1106 38-39 0.18101419307774336 1106 40-41 0.22126537248666267 1106 42-43 0.6219452359930457 1106 44-45 0.34889268912413485 1106 46-47 0.49151878362796 1106 48-49 0.8462394679226648 1106 50-51 0.5794361733556386 1106 52-53 0.5158569074678212 1106 54-55 0.6222054974249076 1106 56-57 0.557766805457824 1106 58-59 0.34706943011962466 1106 60-61 0.17763028318095309 1106 62-63 0.39722645985822425 1106 64-65 0.2514503462389719 1106 66-67 0.5783444075186495 1106 68-69 0.41578187164491354 1106 70-71 0.5597057074608749 1106 72-73 0.6189911280811096 1106 74-75 0.7152696715624387 1106 76-77 0.6036295125203388 1106 78-79 0.3137605063355906 1106 80-81 0.49714457549089275 1106 82-83 0.4415002560296948 1106 84-85 0.5597617099266223 1106 86-87 0.3221892311264156 1106 88-89 0.7995249490541099 1106 90-91 1.0757773857805155 1106 92-93 1.0603280604252454 1106 94-95 0.9389707676942933 1106 96-97 0.9251604233118051 1106 98-99 1.0884844159599076 1106 100-101 1.1855920674744773 1107 1 0.5157508062420426 1107 2 0.5995994259270923 1107 3 0.5022406162935447 1107 4 0.4955997454837018 1107 5 0.4486231279275543 1107 6 0.5812604859368804 1107 7 0.5062467308699681 1107 8 0.4317708392242352 1107 9 0.584218250556404 1107 10-11 0.5629654511204265 1107 12-13 0.5229771634003555 1107 14-15 0.7464837873759365 1107 16-17 0.46789301927267246 1107 18-19 0.6689249263116679 1107 20-21 0.8350578136743536 1107 22-23 0.709875493155927 1107 24-25 0.8573593899632215 1107 26-27 0.8491255964596718 1107 28-29 0.6429137437130379 1107 30-31 0.6685319814399762 1107 32-33 0.8119767715717856 1107 34-35 0.6645280930228807 1107 36-37 0.6638730437426759 1107 38-39 0.6528131407862787 1107 40-41 0.926931910176485 1107 42-43 0.9218084141685665 1107 44-45 0.8532825555333758 1107 46-47 0.9985433676776481 1107 48-49 0.9267499369861767 1107 50-51 0.7143133727764379 1107 52-53 0.5570410744303373 1107 54-55 0.4890186259377032 1107 56-57 0.7117920796000945 1107 58-59 0.7726102353340067 1107 60-61 0.7629285188575423 1107 62-63 0.4550825456371328 1107 64-65 0.6428953921321217 1107 66-67 0.8068697634349036 1107 68-69 0.8626716682471063 1107 70-71 0.8951635021164499 1107 72-73 0.7603972814731357 1107 74-75 0.84012357537231 1107 76-77 0.8438874396098619 1107 78-79 0.9990174561263672 1107 80-81 1.017902201412845 1107 82-83 1.0767005623829355 1107 84-85 1.2175735180888623 1107 86-87 1.2274730267154084 1107 88-89 0.9826906789766028 1107 90-91 1.1598579869987482 1107 92-93 1.233240131460903 1107 94-95 1.2621365375065317 1107 96-97 1.4776643025894813 1107 98-99 1.4938948614884637 1107 100-101 1.9017256741855313 1108 1 0.06745944356847389 1108 2 0.07754247134954184 1108 3 0.19738300273741416 1108 4 0.24460892411396884 1108 5 0.11836895047403573 1108 6 0.0011475181797635514 1108 7 0.08462281936137828 1108 8 0.1799045177368228 1108 9 0.11583980857005116 1108 10-11 0.14286895782789344 1108 12-13 -0.012979532292753504 1108 14-15 0.10054709638982473 1108 16-17 0.17804128945389408 1108 18-19 0.23892139606925866 1108 20-21 0.12752662986640217 1108 22-23 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-0.7730335489683213 2316 46-47 -0.672640000523792 2316 48-49 -0.8307191520158383 2316 50-51 -0.6042836755011578 2316 52-53 -0.8538386277751897 2316 54-55 -0.6846344868240166 2316 56-57 -0.43446867298080605 2316 58-59 -0.4339048738591984 2316 60-61 -0.6961475430043542 2316 62-63 -1.020534961759278 2316 64-65 -0.8388857882148137 2316 66-67 -0.654071789269139 2316 68-69 -0.5870897655443592 2316 70-71 -0.7929275691797883 2316 72-73 -1.0190757636805792 2316 74-75 -1.2154905687251016 2316 76-77 -0.8675514823102937 2316 78-79 -0.7614173215253643 2316 80-81 -0.8104997185178355 2316 82-83 -1.0219581083728961 2316 84-85 -1.023545092355608 2316 86-87 -1.2425095297338586 2316 88-89 -0.7824769444251167 2316 90-91 -0.5650312806571485 2316 92-93 -0.6134447388240645 2316 94-95 -0.8801181839382046 2316 96-97 -0.705085393858532 2316 98-99 -0.4914648119865035 2316 100-101 -0.06293545918923016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3334.0 3 602.0 4 509.0 5 591.0 6 679.0 7 863.0 8 1076.0 9 1399.0 10 1819.0 11 2581.0 12 3963.0 13 5898.0 14 6991.0 15 9455.0 16 13245.0 17 19437.0 18 16250.0 19 8294.0 20 7406.0 21 7335.0 22 7661.0 23 8190.0 24 9024.0 25 9940.0 26 11234.0 27 12850.0 28 14971.0 29 17649.0 30 21378.0 31 26601.0 32 33128.0 33 43592.0 34 60204.0 35 86842.0 36 118893.0 37 165204.0 38 136631.0 39 18560.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.897729440946385 19.024622395919003 15.587970182052649 19.489677981081964 2 15.122874174827949 21.31032169377594 41.8327972866611 21.734006844735013 3 18.690716959622037 27.745641295471113 31.933834289754508 21.629807455152342 4 11.30648308522067 17.700993181469382 38.95463813553748 32.03788559777247 5 11.56045411293187 38.86971659599472 34.34908725756306 15.220742033510342 6 27.776846363814677 38.01451033376397 18.37668791386284 15.83195538855851 7 25.50788633163682 36.517413480449896 20.979340495587053 16.995359692326232 8 26.4737238281922 33.49092937104389 20.130583748141444 19.904763052622467 9 27.554629749352944 16.135253419358797 21.47429645912001 34.83582037216825 10-11 24.07163791152706 27.891654425482553 26.79260521071731 21.24410245227308 12-13 26.471600216963715 26.33983311326492 28.120360072102084 19.068206597669285 14-15 23.06686712266022 25.97755170471605 29.316250742395784 21.639330430227947 16-17 19.157497418366983 29.971102145015188 29.523034740426045 21.348365696191784 18-19 19.031173147079336 26.429488292744125 35.330771957370786 19.208566602805753 20-21 20.740896964650588 24.9476578891147 35.580802962492264 18.730642183742447 22-23 20.011208827691394 25.42563140049536 35.62089838260889 18.942261389204354 24-25 19.40731139298203 25.577220281338903 36.160327127559036 18.855141198120023 26-27 19.335577985028838 25.584317970653718 36.12220167242256 18.957902371894885 28-29 18.868649170462525 25.800363131521763 36.38338974619145 18.94759795182426 30-31 18.89638721610084 25.759548040427415 36.31641114646611 19.027653597005635 32-33 18.81714892446667 25.94160529554019 35.949965867390155 19.291279912602988 34-35 19.34133111118051 25.84068983162371 35.73157095137836 19.086408105817426 36-37 19.46961944600875 25.471501727798824 35.82328463650487 19.23559418968755 38-39 19.20979807009702 25.975852110886162 35.68830576955101 19.126044049465815 40-41 19.112521424474927 26.161057274899907 35.73594201136175 18.990479289263416 42-43 19.149259029584034 26.344549599987726 35.1132655945622 19.392925775866036 44-45 19.16267812838446 26.66355873847332 34.5010707013491 19.67269243179312 46-47 19.55478155689502 27.00996635881412 33.67038430713455 19.764867777156315 48-49 19.770875680058733 27.33356709638891 32.95434497948182 19.941212244070545 50-51 19.819756644076897 27.597449200409795 32.568769140584344 20.014025014928972 52-53 19.875244497772808 28.16219858972041 32.041070146890426 19.921486765616354 54-55 19.50363312852247 28.002699646767 32.193835514379124 20.29983171033141 56-57 19.831552285782678 28.26314039518824 31.776813351803863 20.128493967225218 58-59 20.011809964739914 27.71759284872673 32.07900844500122 20.19158874153213 60-61 19.904586110761173 27.65622518116041 32.57874932218893 19.86043938588948 62-63 19.972502944156876 27.6073727165667 32.66661189165503 19.753512447621397 64-65 19.815166390781982 27.62124228079766 32.724378602157486 19.83921272626287 66-67 19.692893184143585 27.900721044824845 32.26434585612844 20.142039914903126 68-69 19.602356914713845 27.95393532123836 32.385721570962424 20.057986193085366 70-71 19.709965194508687 28.163963772490185 31.989609843260258 20.13646118974087 72-73 19.824795016303934 28.044300250926664 31.78048381027293 20.350420922496472 74-75 19.654366002595513 28.491824239080955 31.607756304311742 20.24605345401179 76-77 19.652803035946693 28.225419480365822 31.75632890591107 20.365448577776416 78-79 19.61571939496292 28.17329219119154 31.518327991537852 20.69266042230769 80-81 19.52783716467855 28.42343800582713 31.51047957410143 20.538245255392887 82-83 19.658848987769325 28.40588251080748 31.43476982110494 20.50049868031826 84-85 19.557333000336282 28.16519720764447 31.143439574727715 21.13403021729153 86-87 19.71146815496811 28.090886435771818 31.242335530549703 20.95530987871037 88-89 19.84550029467116 27.756325571103385 31.164980797080016 21.233193337145437 90-91 19.560538878040365 27.750137622593655 31.532636410693204 21.156687088672772 92-93 19.69552233573828 27.802292181998638 31.5459301828794 20.956255299383685 94-95 19.385251760535414 27.740243180372925 31.426618386250272 21.447886672841392 96-97 19.411655801225418 27.64552771426975 31.425353816086844 21.51746266841799 98-99 19.431220170063167 27.98028218236955 31.06920244067975 21.51929520688754 100-101 19.51181838995593 27.680841577596492 31.57657771151451 21.23076232093307 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1979.0 1 1320.0 2 888.0 3 1111.0 4 884.0 5 796.5 6 1506.0 7 3564.0 8 6113.0 9 6968.0 10 5505.0 11 4017.0 12 3870.5 13 4017.0 14 4033.5 15 3997.0 16 4065.5 17 4148.5 18 4151.0 19 4261.5 20 4611.5 21 5097.0 22 5626.5 23 6271.0 24 7056.5 25 8116.5 26 9567.0 27 10909.0 28 11761.5 29 12767.5 30 14295.5 31 15771.5 32 17535.0 33 20009.0 34 22883.5 35 24570.5 36 26001.0 37 27679.5 38 28173.0 39 28061.5 40 28032.5 41 29032.5 42 29773.0 43 30341.5 44 31444.5 45 31836.0 46 30801.0 47 30891.5 48 35261.5 49 34423.0 50 28362.5 51 24907.0 52 22455.5 53 21383.5 54 20267.5 55 18074.0 56 15845.0 57 13851.0 58 12409.5 59 11034.5 60 9551.5 61 8045.5 62 6398.0 63 4773.0 64 3332.5 65 2444.5 66 1731.0 67 1242.5 68 854.5 69 544.5 70 432.5 71 324.5 72 265.5 73 241.0 74 190.0 75 152.0 76 117.5 77 73.0 78 45.0 79 35.5 80 25.5 81 14.0 82 12.0 83 8.0 84 5.0 85 5.5 86 3.5 87 3.0 88 2.0 89 1.5 90 1.5 91 2.0 92 2.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2568135433050197 2 0.2240009100036969 3 0.22170402567260428 4 0.22553216622442526 5 0.2281571768885311 6 0.18495387637512278 7 0.17795384793750724 8 0.17620384082810336 9 0.18418824826475858 10-11 0.1881804519830862 12-13 0.18451637459777182 14-15 0.18550075359681148 16-17 0.1733053915531532 18-19 0.17735228299364966 20-21 0.16811005794711043 22-23 0.175930402217259 24-25 0.1761491531059345 26-27 0.17434445827436174 28-29 0.18211011482234146 30-31 0.17871947604787145 32-33 0.18211011482234146 34-35 0.18085229721245744 36-37 0.17062569316687848 38-39 0.1629694120632365 40-41 0.16214909623070345 42-43 0.17062569316687848 44-45 0.17089913177772287 46-47 0.1707897563333851 48-49 0.1834226201543944 50-51 0.17795384793750724 52-53 0.18561012904114924 54-55 0.1717194476102559 56-57 0.16149284356467697 58-59 0.16335222611841863 60-61 0.1549850046265813 62-63 0.159360022400091 64-65 0.15979752417744197 66-67 0.15750063984634938 68-69 0.16171159445335245 70-71 0.1635162892849252 72-73 0.1602897136769618 74-75 0.16991475277868318 76-77 0.16253191028588554 78-79 0.16532098411649798 80-81 0.16214909623070345 82-83 0.15011779735355174 84-85 0.14885997974366771 86-87 0.15143030268560465 88-89 0.15312562207283967 90-91 0.15963346101093534 92-93 0.15750063984634938 94-95 0.15536781868176341 96-97 0.15848501884538907 98-99 0.16444598056179605 100-101 0.15586000818128323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 914282.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.227273929392126 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51837979361608 38.43309622527218 2 12.700615764342746 12.75834614135198 3 4.362691290292767 6.57378271520724 4 1.968536364055705 3.9549686078958195 5 1.0921061861318486 2.7426758285414037 6 0.689042935952832 2.0765248975929294 7 0.4576372720319022 1.6090110835852263 8 0.3340497950001642 1.3422728447624424 9 0.24624501728347606 1.1131394343160546 >10 1.4490595857904975 13.822230588180611 >50 0.11904963482706808 4.096285243789526 >100 0.05711552496341867 5.034723694370746 >500 0.0024071677130745816 0.82820248401231 >1k 0.0024071677130745816 1.6971830863482518 >5k 2.188334284613256E-4 0.9087694940695128 >10k+ 4.376668569226512E-4 3.008787630703779 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15239 1.6667723962628598 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12135 1.3272710170385067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8268 0.9043161737844561 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2595 0.2838292780564421 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2242 0.24521974620521897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1388 0.15181311674078676 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.13814118619856894 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1261 0.13792243530989345 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.13464117197976117 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1145 0.1252348837667153 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1124 0.1229379994356227 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1122 0.12271924854694721 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.11331296033390136 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1034 0.11309420944522587 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.1875088867548526E-4 0.0 18-19 0.0 0.0 0.0 3.2812633301322787E-4 1.0937544433774263E-4 20-21 0.0 0.0 0.0 4.375017773509705E-4 1.0937544433774263E-4 22-23 0.0 0.0 0.0 9.843789990396836E-4 1.0937544433774263E-4 24-25 0.0 0.0 0.0 0.0015312562207283967 1.0937544433774263E-4 26-27 0.0 0.0 0.0 0.0020234457202482387 1.0937544433774263E-4 28-29 0.0 0.0 0.0 0.004265642329171962 1.0937544433774263E-4 30-31 0.0 0.0 0.0 0.009789102268227965 1.0937544433774263E-4 32-33 0.0 0.0 0.0 0.015695376262466067 1.0937544433774263E-4 34-35 0.0 0.0 0.0 0.020562583535495612 1.0937544433774263E-4 36-37 0.0 0.0 0.0 0.02832824008347534 1.0937544433774263E-4 38-39 0.0 0.0 0.0 0.034015763189037956 1.0937544433774263E-4 40-41 0.0 0.0 0.0 0.04260173556955075 1.0937544433774263E-4 42-43 0.0 0.0 0.0 0.05906273994238102 1.0937544433774263E-4 44-45 5.4687722168871314E-5 0.0 0.0 0.07798469181281048 1.0937544433774263E-4 46-47 1.0937544433774263E-4 0.0 0.0 0.1016097877897629 1.0937544433774263E-4 48-49 1.0937544433774263E-4 0.0 0.0 0.12807864531949661 1.0937544433774263E-4 50-51 1.0937544433774263E-4 0.0 0.0 0.1597428364552731 1.0937544433774263E-4 52-53 1.0937544433774263E-4 0.0 0.0 0.22203215200561752 1.0937544433774263E-4 54-55 1.0937544433774263E-4 0.0 0.0 0.2725636072896546 1.0937544433774263E-4 56-57 1.0937544433774263E-4 0.0 0.0 0.36378272786733196 1.0937544433774263E-4 58-59 1.0937544433774263E-4 0.0 0.0 0.48431446752752433 1.0937544433774263E-4 60-61 1.0937544433774263E-4 0.0 0.0 0.6067602774636272 1.0937544433774263E-4 62-63 1.0937544433774263E-4 0.0 0.0 0.7690734368608373 1.0937544433774263E-4 64-65 1.0937544433774263E-4 0.0 0.0 0.8818942076952188 1.0937544433774263E-4 66-67 1.0937544433774263E-4 0.0 0.0 1.0743403020074769 1.0937544433774263E-4 68-69 1.0937544433774263E-4 0.0 0.0 1.2951146364032105 1.0937544433774263E-4 70-71 1.0937544433774263E-4 0.0 0.0 1.4721387930638468 1.0937544433774263E-4 72-73 1.0937544433774263E-4 0.0 0.0 1.775491587934576 1.0937544433774263E-4 74-75 1.0937544433774263E-4 0.0 0.0 2.066539645317309 1.0937544433774263E-4 76-77 1.0937544433774263E-4 0.0 0.0 2.2693217191194837 1.0937544433774263E-4 78-79 1.0937544433774263E-4 0.0 0.0 2.4888929236275024 1.0937544433774263E-4 80-81 1.0937544433774263E-4 0.0 0.0 2.857925672823046 1.0937544433774263E-4 82-83 1.0937544433774263E-4 0.0 0.0 3.187747325223509 1.0937544433774263E-4 84-85 1.0937544433774263E-4 0.0 0.0 3.6047958944833214 1.0937544433774263E-4 86-87 1.0937544433774263E-4 0.0 0.0 4.1399699436278965 1.0937544433774263E-4 88-89 1.0937544433774263E-4 0.0 0.0 4.8438009279412695 1.0937544433774263E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTC 40 7.5379085E-6 59.3834 8 TATCACG 40 5.373658E-4 47.524952 2 CAACGTA 40 5.383837E-4 47.506718 9 GGTATCA 7470 0.0 38.117393 1 GTATCAA 11690 0.0 33.91318 1 GACCGGT 85 6.400865E-4 27.945127 7 GTACATG 7495 0.0 27.588915 1 ATCAACG 14360 0.0 27.434557 3 TCAACGC 14405 0.0 27.387854 4 TATCAAC 14410 0.0 27.340864 2 TATAACG 70 0.008369206 27.157118 2 CAACGCA 14560 0.0 27.096292 5 AACGCAG 14785 0.0 26.733572 6 TACATGG 7640 0.0 26.623926 2 GTATAGG 215 5.638867E-11 26.531376 1 ACATGGG 7695 0.0 25.567577 3 GAGTACT 9190 0.0 25.534697 12-13 ACGCAGA 16225 0.0 24.273075 7 CGCAGAG 16215 0.0 24.170855 8 AGTACTT 9340 0.0 24.107422 12-13 >>END_MODULE