FastQCFastQC Report
Thu 26 May 2016
SRR937786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914282
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128651.4071150914050587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111581.2204112079205323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80130.8764254354783316No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25490.278798007616906No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22420.24521974620521897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22100.2417197319864112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.23800096687892794No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14030.1534537484058529No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.14196932675038992No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11550.12632863821009271No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.11539109377631845No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.10281291767747806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91900.032.1569251
CGGGTCG1601.904482E-929.6866266
GTATCAA133050.029.1390171
AGGTAAG2155.820766E-1126.51522
TTAGCGA559.4815245E-525.90832974-75
GAGTACT92450.024.19885612-13
CTGTACG1000.001653670223.7493029
AGGCCGT1000.001653670223.7493026
GTACATG81500.023.6683521
TATCAAC163500.023.535282
AGTACTT93500.023.49529812-13
ATCAACG164800.023.3207993
TCAACGC165650.023.2011364
GTACTTT96050.023.0693414-15
CAACGCA167150.022.9929265
TACATGG82850.022.7640822
AACGCAG169950.022.6383466
GATCGTC1050.002196255122.6183837
CGCTTAT753.0351666E-522.16601658-59
GTCGGGA2154.420508E-822.0923739
Produced by FastQC (version 0.11.5)