##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937786_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 914282 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2231915317156 33.0 31.0 34.0 31.0 34.0 2 32.41667122397685 34.0 31.0 34.0 31.0 34.0 3 32.45840014350058 34.0 31.0 34.0 31.0 34.0 4 36.10427198610494 37.0 35.0 37.0 35.0 37.0 5 36.04383002180946 37.0 35.0 37.0 35.0 37.0 6 36.10695824701788 37.0 35.0 37.0 35.0 37.0 7 36.05208458659364 37.0 35.0 37.0 35.0 37.0 8 36.04610612480613 37.0 35.0 37.0 35.0 37.0 9 37.69971299883406 39.0 37.0 39.0 35.0 39.0 10-11 37.64248940698822 39.0 37.0 39.0 35.0 39.0 12-13 37.53800031062626 39.0 37.0 39.0 35.0 39.0 14-15 38.64473215047436 40.0 38.0 41.0 34.0 41.0 16-17 38.6376730592968 40.0 38.0 41.0 34.5 41.0 18-19 38.73745080839391 40.0 38.0 41.0 35.0 41.0 20-21 38.70369153062184 40.0 38.0 41.0 34.5 41.0 22-23 38.54528744960527 40.0 38.0 41.0 34.0 41.0 24-25 38.57305842179984 40.0 38.0 41.0 34.0 41.0 26-27 38.41227870613224 40.0 38.0 41.0 34.0 41.0 28-29 38.25223508720504 40.0 38.0 41.0 34.0 41.0 30-31 38.158197908304004 40.0 37.0 41.0 34.0 41.0 32-33 37.94931487221667 40.0 36.5 41.0 33.5 41.0 34-35 37.7554288501797 40.0 36.0 41.0 33.0 41.0 36-37 37.5356033477636 40.0 36.0 41.0 33.0 41.0 38-39 37.34816664880201 40.0 35.5 41.0 33.0 41.0 40-41 37.10773645330434 40.0 35.0 41.0 33.0 41.0 42-43 36.79785230377499 40.0 35.0 41.0 31.5 41.0 44-45 36.30210700855972 39.0 35.0 41.0 30.5 41.0 46-47 35.66672591169902 39.0 35.0 41.0 28.0 41.0 48-49 35.09093529129962 39.0 35.0 41.0 24.5 41.0 50-51 34.73630947563225 39.0 35.0 40.5 23.0 41.0 52-53 34.46824393349098 39.0 35.0 40.0 22.0 41.0 54-55 34.23646424188598 38.0 34.0 40.0 20.5 41.0 56-57 33.97619060639934 38.0 34.0 40.0 19.5 41.0 58-59 33.714560168525686 38.0 34.0 40.0 17.5 41.0 60-61 33.3619260796997 38.0 33.5 40.0 13.5 41.0 62-63 33.02080320951303 37.0 33.0 40.0 9.0 41.0 64-65 32.63115701720038 36.5 33.0 39.5 7.5 41.0 66-67 32.200071750291485 36.0 32.0 39.0 3.5 40.5 68-69 31.765281937082868 35.5 31.5 39.0 2.0 40.0 70-71 31.339998490618868 35.0 31.0 38.0 2.0 40.0 72-73 30.84385889692677 35.0 31.0 37.5 2.0 40.0 74-75 30.347084378780288 35.0 30.0 37.0 2.0 39.0 76-77 29.112087955357318 33.5 28.5 35.5 2.0 38.5 78-79 29.672188668266465 34.0 30.0 36.0 2.0 38.5 80-81 29.4537155932196 34.0 30.0 36.0 2.0 37.5 82-83 29.089784114747967 34.0 29.0 35.0 2.0 37.0 84-85 28.704867316648475 34.0 29.0 35.0 2.0 36.5 86-87 28.270367895244576 34.0 29.0 35.0 2.0 36.0 88-89 27.91499121715182 34.0 28.5 35.0 2.0 36.0 90-91 27.408577987973075 33.5 26.5 35.0 2.0 35.0 92-93 26.956803262013253 33.0 26.0 35.0 2.0 35.0 94-95 26.498732885477345 33.0 25.0 35.0 2.0 35.0 96-97 25.9581004547831 33.0 24.5 35.0 2.0 35.0 98-99 25.153946484782594 32.0 19.0 34.5 2.0 35.0 100-101 22.719158312205643 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2933790948492643 1101 2 0.14790490455833094 1101 3 0.0954060363705409 1101 4 0.14050020929737883 1101 5 0.08503731133949088 1101 6 0.08234672366849338 1101 7 0.20473494121815605 1101 8 0.12117071684638603 1101 9 0.1826852720827148 1101 10-11 0.25151744649804897 1101 12-13 0.13701356369254114 1101 14-15 0.29611371251332486 1101 16-17 0.22740207732942963 1101 18-19 0.2858057595109358 1101 20-21 0.2737931311007742 1101 22-23 0.25918980901322186 1101 24-25 0.2682938483853121 1101 26-27 0.10100084808136245 1101 28-29 0.15258244634177487 1101 30-31 0.1020935772791205 1101 32-33 0.06478118206160133 1101 34-35 0.027158948569677932 1101 36-37 0.008596083816840405 1101 38-39 -0.0762316761555013 1101 40-41 0.02864326063723155 1101 42-43 0.1758400264212696 1101 44-45 0.10401173487950643 1101 46-47 -0.07008103361908269 1101 48-49 -0.1685781372844204 1101 50-51 0.07907913837895109 1101 52-53 0.0056089611754686075 1101 54-55 0.02398452255042116 1101 56-57 0.193464231198746 1101 58-59 0.06703080145428686 1101 60-61 0.067007375436269 1101 62-63 0.16968751632486345 1101 64-65 0.27727865758889436 1101 66-67 0.1866804684558332 1101 68-69 0.10737365776900987 1101 70-71 0.2100193434346167 1101 72-73 0.15489961597034707 1101 74-75 0.17618256410678867 1101 76-77 0.08584461535305721 1101 78-79 0.21906903591643712 1101 80-81 0.29128543663676254 1101 82-83 0.3296761227082605 1101 84-85 0.20058646590252138 1101 86-87 0.048965609829966894 1101 88-89 0.16257887284696437 1101 90-91 0.21585616762848758 1101 92-93 0.4730792788516567 1101 94-95 0.3193754858391813 1101 96-97 0.39590207882949713 1101 98-99 0.5514250072622886 1101 100-101 0.802655036520239 1102 1 0.1987820252521928 1102 2 0.20569920606520498 1102 3 0.24083879722245882 1102 4 0.1077662267130961 1102 5 0.04358011584037769 1102 6 0.002316125524124857 1102 7 0.10391899070160093 1102 8 0.13970397999042206 1102 9 0.12951523852346014 1102 10-11 0.14009084914330572 1102 12-13 0.17354270321569487 1102 14-15 0.29301222318429865 1102 16-17 0.3120843611135129 1102 18-19 0.26360620258083145 1102 20-21 0.24249391634347717 1102 22-23 0.293135105233965 1102 24-25 0.1395022557464074 1102 26-27 0.18586162104094228 1102 28-29 0.24290921039607838 1102 30-31 0.19975128384400875 1102 32-33 0.11056132507228966 1102 34-35 0.1951679854409889 1102 36-37 0.0989201061019429 1102 38-39 0.10752882482056947 1102 40-41 0.1774993945233021 1102 42-43 0.3585109483137572 1102 44-45 0.43705444067670385 1102 46-47 0.1722447543653871 1102 48-49 0.2775060679315189 1102 50-51 0.11841201109505306 1102 52-53 0.214193988922176 1102 54-55 0.2154787319338567 1102 56-57 0.28779205905951244 1102 58-59 0.3414816250817623 1102 60-61 0.31324923238172175 1102 62-63 0.34648529439510156 1102 64-65 0.5972253027600942 1102 66-67 0.6349879743232236 1102 68-69 0.6248381792754429 1102 70-71 0.5497492243321638 1102 72-73 0.5371356932663041 1102 74-75 0.5912721651245327 1102 76-77 0.5570664573832822 1102 78-79 0.7985866490418339 1102 80-81 0.856284833447539 1102 82-83 0.8527686738696723 1102 84-85 0.7807322183536591 1102 86-87 0.8796786343304177 1102 88-89 0.7697288585897653 1102 90-91 0.9180331323803017 1102 92-93 0.9226101621400247 1102 94-95 1.0189812194269514 1102 96-97 1.0332287990155002 1102 98-99 0.8849916651552938 1102 100-101 1.0867601778858607 1103 1 0.14139989125715857 1103 2 0.11933347598689892 1103 3 0.08458245135340547 1103 4 -0.01110087778758384 1103 5 0.023876966308172598 1103 6 -0.01695612402144775 1103 7 0.05001439294921539 1103 8 -0.00601822324578194 1103 9 0.20411975158171458 1103 10-11 -0.0325898440608583 1103 12-13 0.0158035908696732 1103 14-15 0.19967037090869866 1103 16-17 0.22173032972219886 1103 18-19 0.08146923581956855 1103 20-21 0.03065240086327492 1103 22-23 0.06650400019837832 1103 24-25 0.226831010148274 1103 26-27 0.22502306242616044 1103 28-29 0.005554205765143649 1103 30-31 0.08342407473865876 1103 32-33 0.07190631499319267 1103 34-35 0.11596906318624178 1103 36-37 0.13528874599337115 1103 38-39 0.060433808897087715 1103 40-41 0.02905682556692568 1103 42-43 0.10987051206466703 1103 44-45 0.07458954416699726 1103 46-47 0.10491601234570425 1103 48-49 0.16226199032990252 1103 50-51 0.20637442373874393 1103 52-53 0.28249658967600055 1103 54-55 0.4108804223495497 1103 56-57 0.30548124644157326 1103 58-59 0.34729784623745985 1103 60-61 0.2630726005337465 1103 62-63 0.4025954685876556 1103 64-65 0.2508105020884841 1103 66-67 0.12154990009945976 1103 68-69 0.28949583666538103 1103 70-71 0.3089795230399588 1103 72-73 0.31520919886057186 1103 74-75 0.3979478955495388 1103 76-77 0.5051758217810374 1103 78-79 0.4970201170933244 1103 80-81 0.3566405116692586 1103 82-83 0.21789543028240743 1103 84-85 0.3189133003583855 1103 86-87 0.39692732553361765 1103 88-89 0.4730597897794979 1103 90-91 0.487993707507961 1103 92-93 0.4668167242020047 1103 94-95 0.46067289810433465 1103 96-97 0.6485784347316574 1103 98-99 0.6744428496349677 1103 100-101 0.7399231447560908 1104 1 0.1606443929134045 1104 2 0.16212610203265143 1104 3 0.07686044977875639 1104 4 0.015817064402490644 1104 5 0.06614975039225612 1104 6 0.05333319629115607 1104 7 0.004476873972599549 1104 8 0.04924822163226139 1104 9 0.23600365725865657 1104 10-11 0.18455005392910806 1104 12-13 0.12762971686885294 1104 14-15 0.12098476702826844 1104 16-17 0.05964074017444432 1104 18-19 0.17906498664888915 1104 20-21 0.20013361769150606 1104 22-23 0.17510360327158025 1104 24-25 0.028943176599604215 1104 26-27 0.15619057333846342 1104 28-29 0.16595928445393326 1104 30-31 0.1829387827634008 1104 32-33 0.20659195390124552 1104 34-35 0.37593542073402375 1104 36-37 0.4017248671284932 1104 38-39 0.3738467664157241 1104 40-41 0.5394031135530071 1104 42-43 0.45743581273313794 1104 44-45 0.3383082372222077 1104 46-47 0.14290905617563254 1104 48-49 0.10508454908278964 1104 50-51 0.24921561082533117 1104 52-53 0.24210256061420665 1104 54-55 0.3207925268656453 1104 56-57 0.41302763218659777 1104 58-59 0.4779768012357053 1104 60-61 0.40671377106181694 1104 62-63 0.593911639971509 1104 64-65 0.4369242734424006 1104 66-67 0.4077006096877156 1104 68-69 0.3513060808159594 1104 70-71 0.38235326552792515 1104 72-73 0.26852253459807685 1104 74-75 0.2946474165466171 1104 76-77 0.41617139476549525 1104 78-79 0.6051094311964818 1104 80-81 0.8066210474487931 1104 82-83 0.8629022805734934 1104 84-85 0.8117796038521554 1104 86-87 0.8299528933196569 1104 88-89 0.8989691748773083 1104 90-91 0.7277491190787018 1104 92-93 0.6393493257761804 1104 94-95 0.6607177175567749 1104 96-97 1.0617688075451177 1104 98-99 1.1623336366502244 1104 100-101 1.399964015638652 1105 1 0.11276331423348296 1105 2 0.15363701029043142 1105 3 0.09197073293523772 1105 4 0.0512021199992887 1105 5 0.09679852310070203 1105 6 0.1204815618033308 1105 7 -0.002452465969255968 1105 8 0.009171904847569579 1105 9 0.042382331779776905 1105 10-11 0.1026905976711987 1105 12-13 0.17063894731792573 1105 14-15 0.07923914563875911 1105 16-17 0.16082451075185844 1105 18-19 0.11672844043361152 1105 20-21 0.17537218267982269 1105 22-23 0.11277856260198149 1105 24-25 0.13805426814572996 1105 26-27 -0.047281500200980986 1105 28-29 0.007487901247230866 1105 30-31 0.014280489466031554 1105 32-33 0.14426341154383238 1105 34-35 0.035346256756980665 1105 36-37 0.04925047447122921 1105 38-39 -0.009609559533387824 1105 40-41 -0.023678291684319674 1105 42-43 -0.03301058242933408 1105 44-45 0.11581749668526697 1105 46-47 -0.33708430062234385 1105 48-49 -0.23683375554004016 1105 50-51 -0.09725488795508141 1105 52-53 -0.10709275152624542 1105 54-55 -0.10604220747630677 1105 56-57 -0.2224846847501709 1105 58-59 -0.2416465572230706 1105 60-61 -0.24433803590914493 1105 62-63 -0.2889353922980433 1105 64-65 -0.3498869695767297 1105 66-67 -0.26801708624171994 1105 68-69 -0.4018729514776034 1105 70-71 -0.314111930696658 1105 72-73 -0.3649598038890751 1105 74-75 -0.24502820710398154 1105 76-77 -0.39474146523302167 1105 78-79 -0.031812064964665154 1105 80-81 0.04353273888406761 1105 82-83 0.07626061929275707 1105 84-85 0.3214952868113379 1105 86-87 0.2252699361342927 1105 88-89 0.23313624340433847 1105 90-91 0.25084775262006787 1105 92-93 0.33398413975651664 1105 94-95 0.1170786835423776 1105 96-97 0.3588314917589095 1105 98-99 0.6021081079453872 1105 100-101 0.40862969249489467 1106 1 0.10344304220134859 1106 2 0.04641486916998616 1106 3 0.005513443088112524 1106 4 -0.03082281795785491 1106 5 0.12930998781555303 1106 6 0.09301910213748243 1106 7 -0.017690091376373118 1106 8 0.020556077248691906 1106 9 0.06454409122967064 1106 10-11 -0.16754569375152784 1106 12-13 -0.1932745098158648 1106 14-15 0.07066514977663729 1106 16-17 0.15451451291644247 1106 18-19 0.07304422725787418 1106 20-21 0.10667324533682176 1106 22-23 -0.033636291439997024 1106 24-25 0.08217290717962555 1106 26-27 0.08353328739347177 1106 28-29 0.02987492787154622 1106 30-31 -0.09865764889583062 1106 32-33 -0.03831374560959233 1106 34-35 -0.19199111041089623 1106 36-37 -0.33578524107296204 1106 38-39 -0.06810477480826194 1106 40-41 -0.08587216743751469 1106 42-43 -0.09043861019634392 1106 44-45 -0.017100204029041777 1106 46-47 0.0902374614451773 1106 48-49 0.03422809603029009 1106 50-51 0.423708802500137 1106 52-53 0.29138222745720554 1106 54-55 0.04058910440924279 1106 56-57 0.00474193654424937 1106 58-59 -0.03762245489388505 1106 60-61 -0.11251395069523085 1106 62-63 0.10764306360244547 1106 64-65 0.05520129991831624 1106 66-67 0.21281506831924446 1106 68-69 0.03366036541164874 1106 70-71 0.17020746040239843 1106 72-73 0.07183324731075658 1106 74-75 0.052056407777239855 1106 76-77 0.013528231172266203 1106 78-79 0.13359067247197132 1106 80-81 -0.1553278370951503 1106 82-83 -0.02886364261116725 1106 84-85 0.025032358992479686 1106 86-87 0.0672620027026305 1106 88-89 -0.14387794716917668 1106 90-91 -0.017635481964859423 1106 92-93 -0.17395306733322968 1106 94-95 0.09800899921845385 1106 96-97 0.2579092806672101 1106 98-99 0.3906036252544638 1106 100-101 0.8430970803521127 1107 1 0.14009174518629663 1107 2 0.3052414724385528 1107 3 0.10648435947522472 1107 4 0.06935828148328937 1107 5 0.12672313786880807 1107 6 0.1575028690058602 1107 7 0.20296800963244266 1107 8 0.17271949755496507 1107 9 0.24428528656576276 1107 10-11 0.31969457414799507 1107 12-13 0.3370147706130311 1107 14-15 0.43575803304727145 1107 16-17 0.3893195118992523 1107 18-19 0.45102050083769996 1107 20-21 0.5192149799822658 1107 22-23 0.5007676161594006 1107 24-25 0.18585333447856556 1107 26-27 0.43389787966044224 1107 28-29 0.49524165423986233 1107 30-31 0.5427734355866534 1107 32-33 0.45022692796863595 1107 34-35 0.6164752884626239 1107 36-37 0.25693747334438655 1107 38-39 0.3318836571657613 1107 40-41 0.4655756308480221 1107 42-43 0.5196820163917693 1107 44-45 0.47178152686676356 1107 46-47 0.6946418876112546 1107 48-49 0.7171584764587848 1107 50-51 0.6329295164467865 1107 52-53 0.6373509698532303 1107 54-55 0.7581427860332326 1107 56-57 0.6481685055076412 1107 58-59 0.603590233236126 1107 60-61 0.7744534713502418 1107 62-63 0.5012780564821284 1107 64-65 0.6520516056732077 1107 66-67 0.6572895408771018 1107 68-69 0.6714315658137338 1107 70-71 0.56046379190213 1107 72-73 0.8783211148448942 1107 74-75 0.9190819491808035 1107 76-77 0.6005696486942362 1107 78-79 0.7775268329244795 1107 80-81 0.7359885643711941 1107 82-83 0.5489313320787907 1107 84-85 0.7721164186515814 1107 86-87 0.9952310961283999 1107 88-89 0.8761506936675509 1107 90-91 0.7967904447736345 1107 92-93 0.7045756792113202 1107 94-95 1.301613237104938 1107 96-97 1.3706008013041675 1107 98-99 1.3901997415889156 1107 100-101 1.9189913087049497 1108 1 0.025210596680764752 1108 2 0.05922358587701382 1108 3 0.09236574333024805 1108 4 0.010097278894448891 1108 5 -0.10221543591325855 1108 6 -0.04831261699084877 1108 7 -0.021418904935693206 1108 8 -0.01707104139537563 1108 9 -0.16083121143376644 1108 10-11 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88-89 -0.2331123448165684 2316 90-91 0.0216269827418607 2316 92-93 0.0900197201865609 2316 94-95 0.07524326281422233 2316 96-97 0.4644325679482222 2316 98-99 0.19983559321612887 2316 100-101 0.9742477460022236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 9.0 5 34.0 6 56.0 7 118.0 8 312.0 9 598.0 10 1078.0 11 1833.0 12 2937.0 13 4360.0 14 5471.0 15 8146.0 16 12024.0 17 18419.0 18 16648.0 19 8313.0 20 6044.0 21 5951.0 22 5964.0 23 6320.0 24 6987.0 25 7899.0 26 9297.0 27 11255.0 28 13379.0 29 16303.0 30 19906.0 31 24790.0 32 31325.0 33 41535.0 34 57926.0 35 85124.0 36 125288.0 37 189059.0 38 154739.0 39 14834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9075421371978 20.324537929495275 15.605806323150679 19.16211361015625 2 15.501125473322237 22.436075521556806 40.14920998116555 21.91358902395541 3 19.255656351103926 27.962051095832575 31.47125285196471 21.311039701098785 4 11.83576147350921 18.619678872992957 37.8427833923962 31.70177626110163 5 11.789099914894361 40.162313298138834 33.280351974166386 14.768234812800413 6 28.229255306349682 38.58164111291702 17.442211483984156 15.746892096749143 7 25.88993330285404 36.93674380552171 20.218925889386423 16.954397002237823 8 27.192922971249573 34.363905228364985 19.2103749171481 19.232796883237338 9 27.80345670154285 16.537567183866685 20.753990563086663 34.904985551503806 10-11 24.515412093861634 28.620053768968432 25.902456791230716 20.962077345939218 12-13 26.68902752157104 26.899583427445446 27.407870853210813 19.0035181977727 14-15 23.14477371314321 26.786319756924016 28.394029413244493 21.674877116688286 16-17 19.432516444598054 30.638468218777138 28.691858748176163 21.23715658844864 18-19 19.438313343147957 27.317118788295076 34.01527099953844 19.22929686901853 20-21 21.053296466516898 25.839073721236993 34.35192861721001 18.755701195036103 22-23 20.41206510528037 26.49689972251177 34.23299167754051 18.85804349466735 24-25 19.930065340890447 26.65763954666066 34.74606303088106 18.66623208156783 26-27 19.874370815003754 26.72392860814763 34.77448872224859 18.627211854600024 28-29 19.18518866902993 26.96858596939864 34.99009595602189 18.856129405549545 30-31 19.39002408447284 26.79862449441201 34.77389908146502 19.03745233965013 32-33 19.196648298883716 27.170829131493345 34.449272762670596 19.18324980695234 34-35 19.654548596603675 26.751483677902442 34.65708610691231 18.936881618581573 36-37 19.923284063341505 26.611701860038806 34.42083514714279 19.044178929476903 38-39 19.76076295976677 26.984468131532797 34.37004904944484 18.884719859255604 40-41 19.367268801656394 27.379960832332085 34.40919548778841 18.843574878223116 42-43 19.506003614420926 27.51374005049687 33.63818158745717 19.342074747625027 44-45 19.538774688772172 27.720495427012672 33.12178299474341 19.61894688947174 46-47 20.011550046922068 28.224333411354486 32.24557631015376 19.51854023156969 48-49 20.306699683467464 28.5686473101297 31.42279953012309 19.701853476279748 50-51 20.27733237666278 28.921437805841087 31.039110471386287 19.762119346109845 52-53 20.288379296540892 29.605854648784515 30.348842042170798 19.756924012503802 54-55 19.823041468605968 29.414447621193464 30.564147604349646 20.19836330585093 56-57 20.15231624378474 29.72851920960929 30.152567807306717 19.966596739299252 58-59 20.306098118523607 29.19777486596039 30.46494407633531 20.03118293918069 60-61 20.0859800367939 29.10245416622005 31.023961972345514 19.787603824640538 62-63 20.11797235426269 29.12586051130833 31.140993697786897 19.615173436642085 64-65 19.92749501794851 29.25557978829289 30.996672798983244 19.820252394775352 66-67 19.770267816713005 29.50495580138294 30.63453070278098 20.090245679123072 68-69 19.949293460814268 29.553512297374823 30.520885001922277 19.976309239888636 70-71 20.013135990864964 29.846261875438866 30.21972433013009 19.92087780356608 72-73 20.054151782491616 29.689964365480236 30.034278264255448 20.2216055877727 74-75 19.91983873684487 30.25899011464734 29.605143708396316 20.216027440111475 76-77 19.908409002911576 29.83237119400798 30.02634854454096 20.232871258539486 78-79 19.881557331326658 29.86797290113991 29.70215972752389 20.548310040009536 80-81 19.84154762047992 30.251695047802592 29.58127752846226 20.325479803255238 82-83 19.794366967459666 30.2805578342904 29.53880229696473 20.386272901285206 84-85 19.653298682889375 30.161089563960036 29.334313345889747 20.851298407260842 86-87 19.709181631050377 29.84369155249693 29.3980413045428 21.049085511909894 88-89 19.684408092907876 29.804808582034862 29.477447877132 21.033335447925257 90-91 19.53057785425303 29.686495840746733 29.582904208369044 21.200022096631194 92-93 19.59307584695198 29.95622636721506 29.46948807163038 20.981209714202585 94-95 19.200531127157706 29.870105722304498 29.490846369063373 21.438516781474426 96-97 19.395656919856236 29.64812825802105 29.453494654822038 21.50272016730068 98-99 19.28612874664281 30.129496969435127 28.89856754334203 21.685806740580038 100-101 18.953441813754125 30.249036738498297 29.371161449410447 21.42635999833713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1773.0 1 1213.0 2 768.0 3 920.5 4 845.0 5 852.0 6 1536.5 7 3185.0 8 4773.0 9 5401.0 10 4861.0 11 4022.5 12 3934.0 13 4108.0 14 4290.0 15 4340.5 16 4285.5 17 4306.0 18 4303.0 19 4435.0 20 4727.0 21 5028.0 22 5640.0 23 6343.5 24 6975.5 25 8038.5 26 9261.5 27 10629.0 28 11718.0 29 12677.5 30 14289.5 31 15644.5 32 17285.5 33 19542.0 34 22616.5 35 24480.0 36 26131.5 37 28191.0 38 28674.0 39 28395.5 40 28034.0 41 28923.5 42 29571.0 43 30347.0 44 31604.0 45 31879.0 46 30564.0 47 30342.5 48 35668.0 49 34815.0 50 28452.5 51 25331.0 52 22541.0 53 21327.0 54 20078.0 55 18092.0 56 15792.5 57 13815.5 58 12792.0 59 11701.0 60 10242.0 61 8596.5 62 6834.5 63 5012.0 64 3359.0 65 2486.0 66 1776.5 67 1286.0 68 921.0 69 541.5 70 405.0 71 323.5 72 281.0 73 273.0 74 217.5 75 162.5 76 118.0 77 70.0 78 49.0 79 36.5 80 21.0 81 16.0 82 11.5 83 8.0 84 6.0 85 3.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026687608418409198 2 0.0 3 0.0 4 2.1875088867548526E-4 5 0.013562555097880086 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038828282739898634 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.843789990396836E-4 24-25 0.0 26-27 4.375017773509705E-4 28-29 9.843789990396836E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.4687722168871314E-5 40-41 8.203158325330696E-4 42-43 0.018156323760065277 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.6406316650661393E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0937544433774263E-4 82-83 0.003500014218807764 84-85 8.75003554701941E-4 86-87 0.0 88-89 0.0 90-91 0.012632863821009272 92-93 0.0036093896631455066 94-95 0.0 96-97 0.0 98-99 0.0014765684985595254 100-101 0.021875088867548527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 914282.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.07087672736167 #Duplication Level Percentage of deduplicated Percentage of total 1 75.06966709024702 36.086647126593434 2 13.136572319990567 12.629730972286762 3 4.637838785936728 6.688349296804234 4 2.2232103875069393 4.274866899073442 5 1.226216864513825 2.9472659867528015 6 0.7520272662913088 2.1690366008102577 7 0.5090560581694391 1.7129539713705833 8 0.3675243908027016 1.4133775747660293 9 0.284478615693211 1.2307622819902957 >10 1.582229090483291 14.252914112730652 >50 0.1336418156368859 4.35609744983633 >100 0.07229214344764509 6.297250871891853 >500 0.002736611102879484 0.8843535804845561 >1k 0.001824407401919656 1.5444122030329026 >5k 2.28050925239957E-4 0.8784337722105798 >10k+ 4.56101850479914E-4 2.6335472993653766 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12865 1.4071150914050587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11158 1.2204112079205323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8013 0.8764254354783316 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2549 0.278798007616906 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2242 0.24521974620521897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2210 0.2417197319864112 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2176 0.23800096687892794 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1403 0.1534537484058529 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1298 0.14196932675038992 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1155 0.12632863821009271 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.11539109377631845 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 940 0.10281291767747806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.1875088867548526E-4 0.0 18-19 0.0 0.0 0.0 3.828140551820992E-4 0.0 20-21 0.0 0.0 0.0 5.468772216887132E-4 0.0 22-23 0.0 0.0 0.0 0.001093754443377426 0.0 24-25 0.0 0.0 0.0 0.0016406316650661393 0.0 26-27 0.0 0.0 0.0 0.002132821164585981 0.0 28-29 0.0 0.0 0.0 0.004539080940016319 0.0 30-31 0.0 0.0 0.0 0.01000785315690345 0.0 32-33 0.0 0.0 0.0 0.01585943942897268 0.0 34-35 0.0 0.0 0.0 0.021000085312846584 0.0 36-37 0.0 0.0 0.0 0.02865636641648857 0.0 38-39 0.0 0.0 0.0 0.03423451407771344 0.0 40-41 0.0 0.0 0.0 0.04287517418039511 0.0 42-43 0.0 0.0 0.0 0.05988305577491408 0.0 44-45 0.0 0.0 0.0 0.07896907081185017 0.0 46-47 0.0 0.0 0.0 0.10259416678880258 0.0 48-49 0.0 0.0 0.0 0.1292817752072118 0.0 50-51 0.0 0.0 0.0 0.16138346812033924 0.0 52-53 0.0 0.0 0.0 0.22460247494755448 0.0 54-55 0.0 0.0 0.0 0.2755714320089425 0.0 56-57 0.0 0.0 0.0 0.3681577456408417 0.0 58-59 0.0 0.0 0.0 0.48967386430007376 0.0 60-61 0.0 0.0 0.0 0.6124478005691898 0.0 62-63 0.0 0.0 0.0 0.7756906512432706 0.0 64-65 0.0 0.0 0.0 0.8895504887988608 0.0 66-67 0.0 0.0 0.0 1.0830356498323275 0.0 68-69 0.0 0.0 0.0 1.3051224895601137 0.0 70-71 0.0 0.0 0.0 1.4822013339429192 0.0 72-73 0.0 0.0 0.0 1.7863197569240126 0.0 74-75 0.0 0.0 0.0 2.081360018025073 0.0 76-77 0.0 0.0 0.0 2.2873139797130424 0.0 78-79 0.0 0.0 0.0 2.5094008194408293 0.0 80-81 0.0 0.0 0.0 2.882042958299518 0.0 82-83 0.0 0.0 0.0 3.216677130250842 0.0 84-85 0.0 0.0 0.0 3.640124163004412 0.0 86-87 0.0 0.0 0.0 4.181587300198407 0.0 88-89 0.0 0.0 0.0 4.89613707805688 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9190 0.0 32.156925 1 CGGGTCG 160 1.904482E-9 29.686626 6 GTATCAA 13305 0.0 29.139017 1 AGGTAAG 215 5.820766E-11 26.5152 2 TTAGCGA 55 9.4815245E-5 25.908329 74-75 GAGTACT 9245 0.0 24.198856 12-13 CTGTACG 100 0.0016536702 23.749302 9 AGGCCGT 100 0.0016536702 23.749302 6 GTACATG 8150 0.0 23.668352 1 TATCAAC 16350 0.0 23.53528 2 AGTACTT 9350 0.0 23.495298 12-13 ATCAACG 16480 0.0 23.320799 3 TCAACGC 16565 0.0 23.201136 4 GTACTTT 9605 0.0 23.06934 14-15 CAACGCA 16715 0.0 22.992926 5 TACATGG 8285 0.0 22.764082 2 AACGCAG 16995 0.0 22.638346 6 GATCGTC 105 0.0021962551 22.618383 7 CGCTTAT 75 3.0351666E-5 22.166016 58-59 GTCGGGA 215 4.420508E-8 22.092373 9 >>END_MODULE