FastQCFastQC Report
Thu 26 May 2016
SRR937785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827918
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145611.7587490548580897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114901.3878186003927924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79260.957341185962861No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.30256619616918584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.2640358103097166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13500.16305962667800433No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.14784072818805727No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.14470032056314755No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10900.13165555042890723No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.12863592771264787No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10480.12658258426559152No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.12175118791957658No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10070.1216304030109262No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9360.11305467449674968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.042.93571
GTATCAA99600.035.162581
GTACATG64950.028.167881
TATCAAC124250.028.0668322
ATCAACG124550.028.0339813
TCAACGC125450.027.8311774
TACATGG65050.027.6812482
CAACGCA126500.027.5626165
AACGCAG129250.027.0112956
ACATGGG66000.025.9839083
GAGTACT85350.025.29408512-13
ACGCAGA141900.024.5363647
CGCAGAG142050.024.4435798
CATGGGG15050.024.3035184
CGACGTT500.001654643923.74829342-43
CATGGGA47650.023.6265534
GTACTTT91950.023.50435314-15
ATGGGGA7550.023.2792875
AGTACTT87750.023.27608712-13
GCAGAGT147450.023.0007559
Produced by FastQC (version 0.11.5)