##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937785_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 827918 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912194202807523 33.0 31.0 34.0 30.0 34.0 2 31.999470962100112 33.0 31.0 34.0 30.0 34.0 3 31.964636594445345 34.0 31.0 34.0 30.0 34.0 4 35.59993501771915 37.0 35.0 37.0 33.0 37.0 5 35.61363081851101 37.0 35.0 37.0 33.0 37.0 6 35.70281960290753 37.0 35.0 37.0 35.0 37.0 7 35.65900970869096 37.0 35.0 37.0 33.0 37.0 8 35.65744675197302 37.0 35.0 37.0 33.0 37.0 9 37.28079713208313 39.0 37.0 39.0 34.0 39.0 10-11 37.180872381081215 39.0 37.0 39.0 34.0 39.0 12-13 37.05259216492455 39.0 37.0 39.0 33.0 39.0 14-15 38.118073891375715 40.0 38.0 41.0 33.0 41.0 16-17 38.09071248601914 40.0 38.0 41.0 33.0 41.0 18-19 38.17712986068669 40.0 38.0 41.0 33.5 41.0 20-21 38.18839788481468 40.0 38.0 41.0 34.0 41.0 22-23 38.00683944545233 40.0 38.0 41.0 33.0 41.0 24-25 37.8407970354552 40.0 37.5 41.0 33.0 41.0 26-27 37.756530840010726 40.0 37.0 41.0 33.0 41.0 28-29 37.76086822608036 40.0 37.0 41.0 33.0 41.0 30-31 37.453540688812176 39.5 37.0 41.0 33.0 41.0 32-33 37.081637915832246 39.0 35.5 41.0 32.5 41.0 34-35 36.99722194710104 39.0 35.0 41.0 32.0 41.0 36-37 36.847333914711356 39.0 35.0 41.0 31.5 41.0 38-39 36.67121140016282 39.0 35.0 41.0 31.0 41.0 40-41 36.37705424933387 39.0 35.0 41.0 30.5 41.0 42-43 36.013469933012686 39.0 35.0 41.0 30.0 41.0 44-45 35.566273471527374 39.0 35.0 41.0 28.0 41.0 46-47 34.89829185982187 39.0 35.0 41.0 24.0 41.0 48-49 34.281067086353964 39.0 34.5 40.0 19.0 41.0 50-51 33.25241871779572 38.0 33.5 39.5 13.0 40.5 52-53 33.319403612435046 38.0 33.5 39.5 9.5 40.5 54-55 33.65162069190427 38.0 34.0 40.0 8.0 41.0 56-57 33.46381948453833 38.0 34.0 40.0 6.5 41.0 58-59 33.24378199290268 38.0 34.0 40.0 2.0 41.0 60-61 32.939827978133096 38.0 33.0 40.0 2.0 41.0 62-63 32.60528880879508 37.0 33.0 40.0 2.0 41.0 64-65 32.237629209656035 37.0 32.0 40.0 2.0 41.0 66-67 31.83867242891204 36.0 32.0 39.0 2.0 41.0 68-69 31.377628581574506 36.0 31.0 39.0 2.0 40.5 70-71 30.946913824799076 35.0 31.0 39.0 2.0 40.0 72-73 30.492850741256984 35.0 30.5 38.0 2.0 40.0 74-75 30.04744310426878 35.0 30.0 37.0 2.0 39.5 76-77 29.56969711976307 35.0 29.0 37.0 2.0 39.0 78-79 29.065345239504396 34.5 29.0 36.0 2.0 39.0 80-81 28.55372935483949 34.0 28.5 36.0 2.0 37.5 82-83 28.05091265076976 34.0 27.0 35.0 2.0 37.0 84-85 27.60733430122307 34.0 26.0 35.0 2.0 37.0 86-87 27.16732635357608 34.0 26.0 35.0 2.0 36.0 88-89 26.717918924337916 33.5 25.0 35.0 2.0 36.0 90-91 26.244162465364926 33.0 24.5 35.0 2.0 35.5 92-93 25.727973664058517 33.0 21.5 35.0 2.0 35.0 94-95 25.16310673279238 32.0 19.0 35.0 2.0 35.0 96-97 24.521848782125765 32.0 7.5 34.5 2.0 35.0 98-99 23.686430298652766 31.5 2.0 34.0 2.0 35.0 100-101 21.517305457786883 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0941994701477995 1101 2 0.28308989500098036 1101 3 0.22731853704100047 1101 4 0.10714988966978467 1101 5 0.0696149203437173 1101 6 0.1581963381585183 1101 7 0.15953738632983772 1101 8 -4.4169721925868544E-4 1101 9 0.22344435492129122 1101 10-11 -0.009162854222509509 1101 12-13 0.1818098023053878 1101 14-15 0.28559503705531597 1101 16-17 0.4560813725078674 1101 18-19 0.2415492726624464 1101 20-21 0.35506125542159594 1101 22-23 0.2374902501705023 1101 24-25 0.2139055245604382 1101 26-27 0.35488939022177846 1101 28-29 0.0703065994848373 1101 30-31 0.15716531818413415 1101 32-33 0.15655979151826926 1101 34-35 0.33841633500315993 1101 36-37 0.3820072746384966 1101 38-39 0.4931668094725268 1101 40-41 0.7466225870812551 1101 42-43 0.5797303134103657 1101 44-45 0.7040314672390195 1101 46-47 0.9481727054229694 1101 48-49 0.8736838527577575 1101 50-51 0.7790632272594067 1101 52-53 0.9289069638378109 1101 54-55 0.8196566480044467 1101 56-57 1.0089684002956574 1101 58-59 1.1444999938435316 1101 60-61 1.197730865224237 1101 62-63 1.1019245941843963 1101 64-65 1.1545072906569551 1101 66-67 1.2714532644164898 1101 68-69 1.2013851778127105 1101 70-71 1.298590064871533 1101 72-73 1.2910924089346736 1101 74-75 1.2718629535293822 1101 76-77 1.3857633005737853 1101 78-79 1.2134423332761841 1101 80-81 1.233289385975123 1101 82-83 1.2210434443412872 1101 84-85 1.614122989405562 1101 86-87 1.6588318512333728 1101 88-89 1.5458592528365571 1101 90-91 1.703135306887468 1101 92-93 1.7537439273197037 1101 94-95 1.712357256099203 1101 96-97 1.8400005834217126 1101 98-99 2.048559170684424 1101 100-101 2.134028414419898 1102 1 0.19248993654275992 1102 2 0.1359176932087358 1102 3 0.10916272843839181 1102 4 0.21493399276648262 1102 5 0.06601699869708 1102 6 0.04374184419003058 1102 7 -0.0396754381042399 1102 8 0.11209076740721713 1102 9 0.20246638071398593 1102 10-11 0.05890599624807891 1102 12-13 0.06415745582468446 1102 14-15 0.1923785429417677 1102 16-17 0.16084038197217865 1102 18-19 0.10137885941455949 1102 20-21 0.003318697787726421 1102 22-23 0.1545127481759394 1102 24-25 0.12542196018749507 1102 26-27 -0.042753866935093754 1102 28-29 -0.23679294944397355 1102 30-31 -0.031249883362747255 1102 32-33 -0.07636570534676679 1102 34-35 0.3025763904341474 1102 36-37 0.4305163323271515 1102 38-39 0.339512484636451 1102 40-41 0.41033342312929477 1102 42-43 0.3963312149354863 1102 44-45 0.6224594154437639 1102 46-47 0.6641884335025026 1102 48-49 0.9807361032527311 1102 50-51 0.826666824718103 1102 52-53 0.8323020346157151 1102 54-55 0.869277180308579 1102 56-57 0.9498426574479097 1102 58-59 0.952638102183947 1102 60-61 1.1612265821311567 1102 62-63 1.315271780120483 1102 64-65 1.3600868855107642 1102 66-67 1.52084486612819 1102 68-69 1.474571826845981 1102 70-71 1.5594617603467782 1102 72-73 1.375480762254746 1102 74-75 1.6199358316969494 1102 76-77 1.6453263803268143 1102 78-79 1.3877143000422834 1102 80-81 1.5178716397262093 1102 82-83 1.5360818772122293 1102 84-85 1.6391031981359454 1102 86-87 1.4776070918535424 1102 88-89 1.4030345718502168 1102 90-91 1.493348234714535 1102 92-93 1.7452992664872795 1102 94-95 1.76096237667549 1102 96-97 1.808559470357622 1102 98-99 1.7903953684150302 1102 100-101 2.337907426106394 1103 1 0.19933810281751718 1103 2 0.25743616464032115 1103 3 0.40858341013535693 1103 4 0.28667285338457305 1103 5 0.17782761159370608 1103 6 0.24280409994211993 1103 7 0.1956940072999629 1103 8 0.25313984677080725 1103 9 0.2544523992508303 1103 10-11 0.3265996083161511 1103 12-13 0.3214857317237687 1103 14-15 0.5729037870638791 1103 16-17 0.5833599134187324 1103 18-19 0.4105744892009824 1103 20-21 0.3270501181742276 1103 22-23 0.43896281509777424 1103 24-25 0.31519944090764085 1103 26-27 0.13633205465733766 1103 28-29 0.2769646023240071 1103 30-31 0.28420897564519976 1103 32-33 0.31838420156647373 1103 34-35 0.32230281600331523 1103 36-37 0.5484144836744846 1103 38-39 0.6434390685369706 1103 40-41 0.839043223906863 1103 42-43 0.8441439277351677 1103 44-45 0.6937051678363417 1103 46-47 1.093073505741181 1103 48-49 1.4165586547355815 1103 50-51 1.4818139353387139 1103 52-53 1.451389767626118 1103 54-55 1.5258268819277916 1103 56-57 1.61938676715274 1103 58-59 1.673649544307466 1103 60-61 1.7445061040509913 1103 62-63 1.8172475675002673 1103 64-65 1.9248358531702472 1103 66-67 1.90892523270551 1103 68-69 1.9747672251734372 1103 70-71 1.915339140768026 1103 72-73 1.931081230539835 1103 74-75 2.016192318418252 1103 76-77 1.7711633225191008 1103 78-79 1.8070588183225098 1103 80-81 1.8316532598718567 1103 82-83 1.7199881832949053 1103 84-85 1.861219521359999 1103 86-87 1.8767111108533072 1103 88-89 1.934477349491953 1103 90-91 1.8311022106092558 1103 92-93 1.800839773483041 1103 94-95 1.8795385518328978 1103 96-97 1.9685521240514063 1103 98-99 2.191442887946476 1103 100-101 2.6448459116938814 1104 1 0.29558519151173 1104 2 0.3452273438212323 1104 3 0.3481099025319381 1104 4 0.35299424598995444 1104 5 0.16515492596029446 1104 6 0.1635553432009189 1104 7 0.32332779127815314 1104 8 0.24762246058877224 1104 9 0.3455942532325622 1104 10-11 0.4024913718967369 1104 12-13 0.40956706942746024 1104 14-15 0.40923493910500497 1104 16-17 0.5165597835521396 1104 18-19 0.5000269109796918 1104 20-21 0.4051440731564 1104 22-23 0.4786562093984159 1104 24-25 0.626466631706549 1104 26-27 0.6620161872145829 1104 28-29 0.5673748874472864 1104 30-31 0.6623078881096731 1104 32-33 0.6666074787927698 1104 34-35 0.5137632125241325 1104 36-37 0.6328063852869263 1104 38-39 0.8623255208071114 1104 40-41 0.924433426366555 1104 42-43 0.8080398478244462 1104 44-45 0.9031772640153903 1104 46-47 0.9749668362749944 1104 48-49 1.0580592943479985 1104 50-51 1.0178023406624632 1104 52-53 1.0832301211432096 1104 54-55 1.1677911919434578 1104 56-57 1.0577356144895091 1104 58-59 0.9282580587347127 1104 60-61 0.9662781026356768 1104 62-63 0.9838248050744838 1104 64-65 0.8028963694482876 1104 66-67 0.8330397874847115 1104 68-69 1.0361593250851797 1104 70-71 1.1384482014903838 1104 72-73 1.1078125583285647 1104 74-75 1.309444407365202 1104 76-77 1.3160474924032712 1104 78-79 1.2646981041339984 1104 80-81 1.2541673255128103 1104 82-83 1.189098694323846 1104 84-85 1.2566589292616435 1104 86-87 1.3316991156902525 1104 88-89 1.5434637096112453 1104 90-91 1.4483048685974111 1104 92-93 1.6691209268850429 1104 94-95 1.6940242179046656 1104 96-97 1.959843480146386 1104 98-99 2.184490262036146 1104 100-101 2.558483167853794 1105 1 0.1308824568710918 1105 2 0.0869619212878554 1105 3 -0.011886084134875574 1105 4 0.06667636770543339 1105 5 -0.016377795711335352 1105 6 0.1220693694287931 1105 7 0.17498682146362654 1105 8 0.0607497761519582 1105 9 0.15400218625448758 1105 10-11 0.08182419241527583 1105 12-13 0.1886628060041673 1105 14-15 0.16453495434080878 1105 16-17 0.1421529579128844 1105 18-19 0.016934935044822907 1105 20-21 0.1548068416874102 1105 22-23 0.03014578773831289 1105 24-25 -0.036335343745228954 1105 26-27 -0.09021994541864586 1105 28-29 0.25515800394914123 1105 30-31 0.24553066897796327 1105 32-33 0.32541284328146247 1105 34-35 0.2679762833557575 1105 36-37 0.4845155821371989 1105 38-39 0.3670151749544033 1105 40-41 0.5468215082534087 1105 42-43 0.7372658319271892 1105 44-45 0.7118490365337138 1105 46-47 0.9326020168564071 1105 48-49 1.0291837336890666 1105 50-51 0.8721350682797677 1105 52-53 0.8327535378275215 1105 54-55 0.8865475789030981 1105 56-57 0.987704808441265 1105 58-59 1.0020272103235115 1105 60-61 1.0127194280761174 1105 62-63 1.0716369067604603 1105 64-65 1.3648038918467726 1105 66-67 1.2677599503963144 1105 68-69 1.2547410577302998 1105 70-71 1.1619150524730948 1105 72-73 1.1146593790626333 1105 74-75 1.1218861118426737 1105 76-77 1.0233045649739978 1105 78-79 1.0238609525600673 1105 80-81 1.093734331454776 1105 82-83 1.1528913685043634 1105 84-85 1.262366780902095 1105 86-87 1.257974556692396 1105 88-89 1.0038394765109082 1105 90-91 1.1073183318438282 1105 92-93 1.4007943010205963 1105 94-95 1.6043559496207749 1105 96-97 1.7119105325389654 1105 98-99 2.0037063242874034 1105 100-101 2.248908395368911 1106 1 0.2265110879077632 1106 2 0.22562694559858087 1106 3 0.28859486646635446 1106 4 0.2355214370405747 1106 5 0.1728967075900485 1106 6 0.1974703516839753 1106 7 0.09391304353611218 1106 8 0.11379120528236797 1106 9 0.2398152840430967 1106 10-11 0.12649592301274737 1106 12-13 0.1840971968673344 1106 14-15 0.23276642124891112 1106 16-17 0.0880078515545506 1106 18-19 0.2889308361735914 1106 20-21 0.46639738269980313 1106 22-23 0.3453658300956519 1106 24-25 0.17661294836251784 1106 26-27 0.3344694369958674 1106 28-29 0.38416804583273034 1106 30-31 0.3907177413907661 1106 32-33 0.31013068618029394 1106 34-35 0.3445221772027125 1106 36-37 0.4003000966157302 1106 38-39 0.1865393830352815 1106 40-41 0.5086341007017339 1106 42-43 0.40294024188098376 1106 44-45 0.2519920057237499 1106 46-47 0.4770175680019335 1106 48-49 0.7856790233209949 1106 50-51 0.7841137387084203 1106 52-53 0.8565369924951725 1106 54-55 0.8547137726905731 1106 56-57 0.7292443065751897 1106 58-59 0.7524338513371376 1106 60-61 0.7594702479381041 1106 62-63 0.8514761102880186 1106 64-65 0.834142194359611 1106 66-67 1.02773647312873 1106 68-69 1.0766445763375287 1106 70-71 1.299336904580347 1106 72-73 1.5482580160475798 1106 74-75 1.5953408321245384 1106 76-77 1.6398706022463543 1106 78-79 1.3611176917465748 1106 80-81 1.2540868764923516 1106 82-83 0.9994214529207568 1106 84-85 1.2249821274549895 1106 86-87 1.1795520606272092 1106 88-89 1.3313806977148452 1106 90-91 1.5453658934892012 1106 92-93 1.3154224986577816 1106 94-95 1.651649132966302 1106 96-97 1.857416074612928 1106 98-99 2.3445522077818985 1106 100-101 2.896795002622998 1107 1 0.07727032074973295 1107 2 0.0746891318932903 1107 3 0.10446214601408954 1107 4 0.06656703327875846 1107 5 0.023966607732702983 1107 6 -0.0046218974838794225 1107 7 0.0766678382737922 1107 8 0.08425667788763747 1107 9 0.14354434834277896 1107 10-11 0.1476899844065258 1107 12-13 0.10731799255602681 1107 14-15 0.21687061103070704 1107 16-17 0.3276306126894326 1107 18-19 0.42810344672994205 1107 20-21 0.2986116797357923 1107 22-23 0.40461346574841883 1107 24-25 0.38086296157116095 1107 26-27 0.34813457145985893 1107 28-29 0.4099618853212945 1107 30-31 0.3491961056323305 1107 32-33 0.3252625084447871 1107 34-35 0.5668638055643029 1107 36-37 0.6005494758094727 1107 38-39 0.40632910142700496 1107 40-41 0.3497954046590408 1107 42-43 0.30714693073817045 1107 44-45 0.6403060544372252 1107 46-47 0.7757590484267212 1107 48-49 0.9053324285093538 1107 50-51 0.4596217431465206 1107 52-53 0.8226975039338598 1107 54-55 1.1190007701016782 1107 56-57 1.1875599403259187 1107 58-59 0.9785859092438258 1107 60-61 0.9148514493963305 1107 62-63 0.6162808865335876 1107 64-65 0.7890214673558624 1107 66-67 0.8386277924080652 1107 68-69 0.7596392400454555 1107 70-71 1.055048850477739 1107 72-73 0.8763321957908019 1107 74-75 0.8578790051049374 1107 76-77 0.8397511961069739 1107 78-79 1.00359034985404 1107 80-81 1.127308200526798 1107 82-83 1.3750425233492471 1107 84-85 1.4242117993522783 1107 86-87 1.395243929386126 1107 88-89 1.2324331624477658 1107 90-91 0.9467607262677724 1107 92-93 1.1760628537061208 1107 94-95 1.4864333037276474 1107 96-97 1.599859145358426 1107 98-99 1.8434384549420422 1107 100-101 2.522840334688304 1108 1 0.21002545833245634 1108 2 0.25380210066143505 1108 3 0.3425348268453945 1108 4 0.26181839749563807 1108 5 0.2515528146292567 1108 6 0.38983996249522335 1108 7 0.17931499954163854 1108 8 0.16111723750682216 1108 9 0.3121011810375407 1108 10-11 0.3412160496567296 1108 12-13 0.3502995320892879 1108 14-15 0.4229358612338885 1108 16-17 0.34563130931081787 1108 18-19 0.43079937149483527 1108 20-21 0.5794859395755694 1108 22-23 0.4412185975259675 1108 24-25 0.45679933681788043 1108 26-27 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-1.4235515620104096 2316 48-49 -1.4946842606234583 2316 50-51 -1.2132186188792815 2316 52-53 -1.4135846707885271 2316 54-55 -1.739418897399446 2316 56-57 -1.485659136636663 2316 58-59 -1.5274003543160894 2316 60-61 -1.437276415036557 2316 62-63 -1.226836129962976 2316 64-65 -1.1586324257053136 2316 66-67 -1.2438537532623855 2316 68-69 -1.0210321766051607 2316 70-71 -1.2484507322939393 2316 72-73 -1.2353835754397018 2316 74-75 -1.035478720037883 2316 76-77 -1.0643684521374581 2316 78-79 -1.241204566379352 2316 80-81 -1.143018521342384 2316 82-83 -0.9987465516876632 2316 84-85 -1.462824595795034 2316 86-87 -1.5049293808391795 2316 88-89 -1.7180732885439518 2316 90-91 -1.663156469957915 2316 92-93 -1.9460361256892185 2316 94-95 -2.074091762066047 2316 96-97 -2.17794687556718 2316 98-99 -2.31050852496681 2316 100-101 -1.9188966189030694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3034.0 3 537.0 4 493.0 5 547.0 6 598.0 7 754.0 8 861.0 9 1152.0 10 1570.0 11 2188.0 12 3533.0 13 5400.0 14 6090.0 15 8680.0 16 11918.0 17 17814.0 18 15064.0 19 7847.0 20 6670.0 21 6425.0 22 6854.0 23 7172.0 24 7911.0 25 8805.0 26 9838.0 27 11318.0 28 13133.0 29 15334.0 30 18297.0 31 22872.0 32 29474.0 33 37973.0 34 52214.0 35 76687.0 36 106883.0 37 152212.0 38 130989.0 39 18773.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.86017567330335 18.98954556185795 15.625843710084567 19.524435054754136 2 15.07397893095542 21.22339433881406 41.92393643937505 21.778690290855472 3 18.715622760637576 27.680819437181647 31.99540507040071 21.608152731780063 4 11.282990368252754 17.827809824430606 38.790234149010075 32.09896565830657 5 11.466737348137713 38.83414302347254 34.4242969977319 15.274822630657848 6 27.810125356952714 37.94165335656551 18.451067228110933 15.797154058370843 7 25.337598714577997 36.61879808687004 21.011640667441824 17.03196253111014 8 26.38887376382884 33.48615694004305 20.226440298967987 19.89852899716012 9 27.619403716516683 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19.211881669720896 34-35 19.16785393431763 25.7776527491674 36.08525337955359 18.969239936961383 36-37 19.353490307859452 25.476138049182985 36.05588799655434 19.114483646403222 38-39 19.10670200351715 25.970951753826128 35.90862427656147 19.013721966095257 40-41 18.98177414310525 26.082532661541318 36.011326660177815 18.92436653517562 42-43 19.025825650692784 26.307510909158005 35.330366147217525 19.33629729293169 44-45 19.020993488534156 26.61649377769067 34.837249649745566 19.525263084029607 46-47 19.474043662131155 26.938974764427865 33.93006969317701 19.656911880263962 48-49 19.630064307709063 27.30560983430027 33.162693059244155 19.901632798746512 50-51 19.62061119181254 27.716655100918462 32.74554728777659 19.91718641949241 52-53 19.740619620821825 28.135359408513512 32.26880689260854 19.85521407805613 54-55 19.414341766653802 27.965646449040136 32.44150495509502 20.17850682921104 56-57 19.592043129254087 28.284912926739423 31.997268432185322 20.125775511821168 58-59 19.882503953030618 27.741140916619184 32.3327054456853 20.043649684664903 60-61 19.790353451884616 27.51078600517341 32.894835542155555 19.804025000786414 62-63 19.88311027573319 27.601268231124898 32.86535903269851 19.650262460443397 64-65 19.761122705138675 27.63082599885068 32.92943774007198 19.67861355593866 66-67 19.558611895326546 27.827062197576314 32.44818985870814 20.166136048389 68-69 19.452137332263792 27.953554719117847 32.5709812575845 20.023326691033862 70-71 19.630002443905116 28.11718887998974 32.15955312831954 20.093255547785603 72-73 19.670489939937852 27.98179927543114 32.03286389804497 20.31484688658604 74-75 19.472050609970402 28.303812262077667 31.872896771122118 20.351240356829813 76-77 19.51510927766697 28.20723161832097 31.92171040253698 20.355948701475082 78-79 19.54052333924609 28.012177208389495 31.81900680249062 20.6282926498738 80-81 19.457081461979246 28.394571782561563 31.666093967786896 20.482252787672294 82-83 19.532041683681957 28.315682804724958 31.63434517597592 20.517930335617162 84-85 19.42719354600636 28.138463716248836 31.36043952587151 21.073903211873297 86-87 19.530765107914107 28.134614663348405 31.412507849013416 20.922112379724076 88-89 19.722895567696273 27.694532985929417 31.3986556750874 21.18391577128691 90-91 19.272273232678256 27.71654372131796 31.89273325840059 21.11844978760319 92-93 19.57242801963637 27.80433802868023 31.67148098520618 20.95175296647722 94-95 19.09559290126388 27.71730155110179 31.693992376668323 21.49311317096601 96-97 19.202948520658516 27.565135545812648 31.722486764805513 21.509429168723322 98-99 19.336627231032967 28.020612669811356 31.22454115093657 21.418218948219106 100-101 19.26505833148836 27.736643885749405 31.77157623864432 21.226721544117915 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1924.0 1 1261.0 2 810.5 3 1023.5 4 821.5 5 696.5 6 1354.5 7 3356.0 8 5831.5 9 6750.0 10 5345.5 11 3757.0 12 3511.5 13 3701.5 14 3812.0 15 3806.5 16 3784.5 17 3786.0 18 3870.0 19 4036.5 20 4263.0 21 4677.0 22 5249.0 23 5797.5 24 6514.5 25 7506.5 26 8780.5 27 10115.0 28 10855.0 29 11617.5 30 12928.5 31 14328.5 32 15992.0 33 18152.5 34 20682.5 35 22292.0 36 23701.0 37 25366.5 38 25677.0 39 25190.5 40 25217.5 41 26197.0 42 26821.0 43 27267.5 44 28242.5 45 28745.5 46 27700.0 47 27664.0 48 32015.0 49 31391.0 50 25749.5 51 22492.5 52 20085.5 53 18940.5 54 18090.0 55 16314.0 56 14034.0 57 12176.0 58 11055.5 59 9767.0 60 8342.0 61 7121.5 62 5734.0 63 4218.0 64 2925.0 65 2117.5 66 1482.0 67 1096.5 68 769.5 69 499.5 70 371.0 71 287.5 72 238.5 73 219.5 74 171.0 75 110.5 76 92.0 77 65.5 78 41.0 79 23.5 80 12.5 81 8.5 82 7.5 83 6.0 84 6.0 85 3.5 86 3.0 87 1.5 88 3.0 89 4.0 90 1.5 91 1.0 92 1.0 93 1.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2358929265941796 2 0.22610934899349935 3 0.2162049864841687 4 0.2043680654364321 5 0.20243550689802614 6 0.17852009498525215 7 0.17139378537488012 8 0.17815774025930103 9 0.18105657806691 10-11 0.1856464045956242 12-13 0.1843781630547953 14-15 0.17870127234822772 16-17 0.17821813271362624 18-19 0.1796675516174307 20-21 0.16934044192782377 22-23 0.16541493239668664 24-25 0.16342198140395547 26-27 0.17296398918733497 28-29 0.17936558934580477 30-31 0.17344712882193647 32-33 0.17737263835307363 34-35 0.17399066091086315 36-37 0.16571689466831258 38-39 0.1686761249302467 40-41 0.16577728712263776 42-43 0.17314516655031054 44-45 0.16620003430291405 46-47 0.1650525776707355 48-49 0.17187692500948162 50-51 0.17918441198282922 52-53 0.1842573781461449 54-55 0.16849494756727115 56-57 0.15538978497870562 58-59 0.1614894228655495 60-61 0.16716631357211706 62-63 0.1706086834686527 64-65 0.16221413231745171 66-67 0.1650525776707355 68-69 0.1687365173845719 70-71 0.16571689466831258 72-73 0.16396551349288213 74-75 0.16444865312748363 76-77 0.17181653255515641 78-79 0.17586282699494393 80-81 0.17175614010083123 82-83 0.17580243454061875 84-85 0.16801180793266968 86-87 0.16680395884616592 88-89 0.16988397401675046 90-91 0.16897808720187263 92-93 0.16795141547834447 94-95 0.16626042675723923 96-97 0.16112706813959837 98-99 0.16173099268285024 100-101 0.16293884176935397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 827918.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.09441774519501 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06497285854932 39.37589916756834 2 12.656758192071832 12.933793807312746 3 4.14070232335113 6.347003228034065 4 1.8928068554568158 3.86847456734718 5 1.0472779101198837 2.675502751749006 6 0.639034224266963 1.9590648964903705 7 0.45013132789057714 1.6099438675208348 8 0.29096234915977676 1.1893241452874337 9 0.24370791070945735 1.120690241783793 >10 1.401456031205617 13.502281262381874 >50 0.11289638115179568 3.9613857432451174 >100 0.053601447005711206 4.783939412877608 >500 0.0026089199863821803 0.8973597625758518 >1k 0.0023717454421656185 1.657903971883893 >5k 2.3717454421656186E-4 0.9604960807800408 >10k+ 4.743490884331237E-4 3.1569370931618526 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14561 1.7587490548580897 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11490 1.3878186003927924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7926 0.957341185962861 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2505 0.30256619616918584 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2186 0.2640358103097166 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1350 0.16305962667800433 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1224 0.14784072818805727 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 0.14470032056314755 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1090 0.13165555042890723 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1065 0.12863592771264787 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1048 0.12658258426559152 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.12175118791957658 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1007 0.1216304030109262 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 936 0.11305467449674968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.2078490865037359E-4 0.0 20-21 0.0 0.0 0.0 1.811773629755604E-4 0.0 22-23 0.0 0.0 0.0 3.623547259511208E-4 0.0 24-25 0.0 0.0 0.0 6.643169975770547E-4 0.0 26-27 0.0 0.0 0.0 0.0016305962667800434 0.0 28-29 0.0 0.0 0.0 0.0034423698965356476 0.0 30-31 0.0 0.0 0.0 0.009179653057428391 0.0 32-33 0.0 0.0 0.0 0.015520860761573005 0.0 34-35 0.0 0.0 0.0 0.020473042016238324 0.0 36-37 0.0 0.0 0.0 0.027720136535260738 0.0 38-39 0.0 0.0 0.0 0.03708096695566469 0.0 40-41 0.0 0.0 0.0 0.04493198601793898 0.0 42-43 0.0 0.0 0.0 0.0617210883203409 0.0 44-45 0.0 0.0 0.0 0.08213373788225403 0.0 46-47 0.0 0.0 0.0 0.10629071961232876 0.0 48-49 0.0 0.0 0.0 0.13419203351056508 0.0 50-51 0.0 0.0 0.0 0.17000475892540085 0.0 52-53 0.0 0.0 0.0 0.2396372587623412 0.0 54-55 0.0 0.0 0.0 0.29133319966470106 0.0 56-57 0.0 0.0 0.0 0.38717602467877255 0.0 58-59 0.0 0.0 0.0 0.5164158769346723 0.0 60-61 0.0 0.0 0.0 0.6448706272843445 0.0 62-63 0.0 0.0 0.0 0.8241154317214989 0.0 64-65 0.0 0.0 0.0 0.9351771552255175 0.0 66-67 0.0 0.0 0.0 1.1310298846020983 0.0 68-69 0.0 0.0 0.0 1.3613063129440355 0.0 70-71 0.0 0.0 0.0 1.5382562041168328 0.0 72-73 0.0 0.0 0.0 1.85096833261265 0.0 74-75 0.0 0.0 0.0 2.155467087320242 0.0 76-77 0.0 0.0 0.0 2.361465749023454 0.0 78-79 0.0 0.0 0.0 2.593493558540822 0.0 80-81 0.0 0.0 0.0 2.9842931304791054 0.0 82-83 0.0 0.0 0.0 3.3346297580195143 0.0 84-85 0.0 0.0 0.0 3.775675851956353 0.0 86-87 0.0 0.0 0.0 4.335272333733534 0.0 88-89 0.0 0.0 0.0 5.078763838930909 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6220 0.0 42.9357 1 GTATCAA 9960 0.0 35.16258 1 GTACATG 6495 0.0 28.16788 1 TATCAAC 12425 0.0 28.066832 2 ATCAACG 12455 0.0 28.033981 3 TCAACGC 12545 0.0 27.831177 4 TACATGG 6505 0.0 27.681248 2 CAACGCA 12650 0.0 27.562616 5 AACGCAG 12925 0.0 27.011295 6 ACATGGG 6600 0.0 25.983908 3 GAGTACT 8535 0.0 25.294085 12-13 ACGCAGA 14190 0.0 24.536364 7 CGCAGAG 14205 0.0 24.443579 8 CATGGGG 1505 0.0 24.303518 4 CGACGTT 50 0.0016546439 23.748293 42-43 CATGGGA 4765 0.0 23.626553 4 GTACTTT 9195 0.0 23.504353 14-15 ATGGGGA 755 0.0 23.279287 5 AGTACTT 8775 0.0 23.276087 12-13 GCAGAGT 14745 0.0 23.000755 9 >>END_MODULE