FastQCFastQC Report
Thu 26 May 2016
SRR937785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827918
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117411.4181356124640363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102951.243480634555596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72090.8707384064605432No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22780.27514802190555104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21470.2593251988723521No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20980.25340673834848376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.2426568814786005No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.1518266301735196No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12460.15049799617836548No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9820.11861078029466686No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.11365859904000156No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.10810249324208435No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.10363345162202053No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT2150.033.1373444
GGTATCA80000.031.6102311
GTATCAA113750.027.6220591
CGTATAG700.00836084927.1623881
AGGTAAG2155.820766E-1126.5098742
TATAACG908.9488784E-426.3871462
GTACATG77400.025.2410181
TACATGG77700.024.757052
GAGTACT78800.024.08285112-13
GTATAGA3400.023.7670881
CGCACGT500.001654589123.7484356-57
TGGGACG1000.001653892723.748436
AGTACTT79200.023.48139612-13
TAAGGTG3250.023.3830685
ACATGGG78200.023.3232653
GTCGGGA1852.5414738E-723.106582
GTACTTT83350.022.62366114-15
CATGGGG18050.022.3669414
TATCAAC141600.022.2389952
ATCAACG142450.022.1062953
Produced by FastQC (version 0.11.5)