##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937785_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 827918 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.266575916938635 33.0 31.0 34.0 31.0 34.0 2 32.4503574025447 34.0 31.0 34.0 31.0 34.0 3 32.476834662369946 34.0 31.0 34.0 31.0 34.0 4 36.097068791836875 37.0 35.0 37.0 35.0 37.0 5 36.04682106198923 37.0 35.0 37.0 35.0 37.0 6 36.1194490275607 37.0 35.0 37.0 35.0 37.0 7 36.07555941530441 37.0 35.0 37.0 35.0 37.0 8 36.058695426358646 37.0 35.0 37.0 35.0 37.0 9 37.722704422418644 39.0 37.0 39.0 35.0 39.0 10-11 37.65869325223029 39.0 37.0 39.0 35.0 39.0 12-13 37.552532376394765 39.0 37.0 39.0 35.0 39.0 14-15 38.671865450443164 40.0 38.0 41.0 34.0 41.0 16-17 38.66408931802425 40.0 38.0 41.0 34.5 41.0 18-19 38.75390739179484 40.0 38.0 41.0 35.0 41.0 20-21 38.722804673892824 40.0 38.0 41.0 34.5 41.0 22-23 38.65474902103831 40.0 38.0 41.0 34.0 41.0 24-25 38.6306542435362 40.0 38.0 41.0 34.0 41.0 26-27 38.42223988365998 40.0 38.0 41.0 34.0 41.0 28-29 38.352629729031136 40.0 38.0 41.0 34.0 41.0 30-31 38.23004935271368 40.0 37.5 41.0 34.0 41.0 32-33 37.967730499880425 40.0 36.5 41.0 34.0 41.0 34-35 37.755253539601746 40.0 36.0 41.0 33.0 41.0 36-37 37.527572175022165 40.0 36.0 41.0 33.0 41.0 38-39 37.33903236793982 40.0 35.5 41.0 33.0 41.0 40-41 37.10040849456105 40.0 35.0 41.0 33.0 41.0 42-43 36.78764986387541 40.0 35.0 41.0 31.5 41.0 44-45 36.273490249034325 39.0 35.0 41.0 30.5 41.0 46-47 35.644189400399554 39.0 35.0 41.0 28.0 41.0 48-49 35.04382680410379 39.0 35.0 41.0 24.0 41.0 50-51 34.684977256201705 39.0 35.0 41.0 22.5 41.0 52-53 34.383227566014995 39.0 35.0 40.0 20.5 41.0 54-55 34.1403738051353 38.0 34.0 40.0 19.5 41.0 56-57 33.878870250435426 38.0 34.0 40.0 17.5 41.0 58-59 33.63065243176257 38.0 34.0 40.0 13.5 41.0 60-61 33.271408521133736 37.5 33.5 40.0 8.5 41.0 62-63 32.883897922258 37.0 33.0 40.0 7.0 41.0 64-65 32.49221360086385 36.5 32.5 39.5 2.0 41.0 66-67 32.08359704705055 36.0 32.0 39.0 2.0 40.5 68-69 31.616574950659366 35.5 31.5 39.0 2.0 40.0 70-71 31.184739309931658 35.0 31.0 38.0 2.0 40.0 72-73 30.72254377849014 35.0 31.0 37.5 2.0 40.0 74-75 30.239238064639252 35.0 30.0 37.0 2.0 39.0 76-77 28.986579588799856 33.5 28.5 35.5 2.0 38.0 78-79 29.537868484560065 34.0 30.0 36.0 2.0 38.5 80-81 29.339307757531543 34.0 30.0 36.0 2.0 37.5 82-83 28.94754613379586 34.0 29.0 35.0 2.0 37.0 84-85 28.553437055360554 34.0 29.0 35.0 2.0 36.5 86-87 28.231102597117108 34.0 29.0 35.0 2.0 36.0 88-89 27.851045031029642 34.0 28.5 35.0 2.0 36.0 90-91 27.37164791682268 34.0 26.5 35.0 2.0 35.0 92-93 26.950319355298472 33.0 26.0 35.0 2.0 35.0 94-95 26.482074915631742 33.0 25.0 35.0 2.0 35.0 96-97 25.999327831983358 33.0 24.5 35.0 2.0 35.0 98-99 25.282938044588956 32.5 20.5 34.5 2.0 35.0 100-101 23.064665220468694 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2426482307752238 1101 2 0.21289356269490156 1101 3 0.21810243383275463 1101 4 0.13886978730539568 1101 5 0.13964840687467728 1101 6 0.15496173430545213 1101 7 0.13229511794509108 1101 8 0.26185143142587464 1101 9 0.25823975837401747 1101 10-11 0.19496548915847 1101 12-13 0.1205228623829413 1101 14-15 0.34032340964866137 1101 16-17 0.2268916930551157 1101 18-19 0.2778028160283412 1101 20-21 0.328889707536014 1101 22-23 0.3093598135453206 1101 24-25 0.303598370447574 1101 26-27 0.19775198346453493 1101 28-29 0.28168823349873406 1101 30-31 0.4726418137296875 1101 32-33 0.3237474786868759 1101 34-35 0.34470307597006666 1101 36-37 0.23011904938997674 1101 38-39 0.38493140610763277 1101 40-41 0.2887319133166386 1101 42-43 0.23521554962849223 1101 44-45 0.3881037456812493 1101 46-47 0.4675883124250717 1101 48-49 0.5151781357146064 1101 50-51 0.503009469216309 1101 52-53 0.47808881973783457 1101 54-55 0.5131043676812723 1101 56-57 0.5220424960092487 1101 58-59 0.4220852469781917 1101 60-61 0.7208385754674467 1101 62-63 0.536385166626637 1101 64-65 0.7625499543571692 1101 66-67 0.5807209424165691 1101 68-69 0.42630520019265816 1101 70-71 0.7343290535237763 1101 72-73 0.6164661697460225 1101 74-75 0.40444917961595905 1101 76-77 0.489662153144252 1101 78-79 0.7260983247469674 1101 80-81 0.7914081396817458 1101 82-83 0.91066292786509 1101 84-85 0.9342450755003142 1101 86-87 1.0045708446445225 1101 88-89 1.0023807823550506 1101 90-91 1.000797812577865 1101 92-93 1.132899616062776 1101 94-95 1.121602634551408 1101 96-97 0.728993489239226 1101 98-99 0.9781221372209217 1101 100-101 1.409969469886029 1102 1 0.06661704004293512 1102 2 0.06667504547380076 1102 3 0.06333161904971263 1102 4 0.09022030033522555 1102 5 0.05043414728420004 1102 6 0.04538059935908478 1102 7 -0.004278531040341704 1102 8 0.0709501686425682 1102 9 0.18218881495158712 1102 10-11 0.17902406514382818 1102 12-13 0.0888429423813335 1102 14-15 -0.040389631602053555 1102 16-17 0.09214593949770489 1102 18-19 0.19412663977879419 1102 20-21 0.03972112604150624 1102 22-23 0.07186457811022962 1102 24-25 0.09719626123062852 1102 26-27 0.12914726411476352 1102 28-29 0.15313564273280633 1102 30-31 0.14639758714780982 1102 32-33 -0.011598417151638785 1102 34-35 -0.12063982610134616 1102 36-37 -0.22440925482614915 1102 38-39 -0.16956827213956416 1102 40-41 0.014880154169830462 1102 42-43 -0.12674092346696142 1102 44-45 -0.08729721078104546 1102 46-47 0.02583868703175085 1102 48-49 -0.10474615592768544 1102 50-51 -0.2805384688651813 1102 52-53 0.034142864949885166 1102 54-55 -0.0858990473736796 1102 56-57 -0.1617134625903418 1102 58-59 -0.17965190952947552 1102 60-61 -0.2285204651690833 1102 62-63 -0.15200380087864573 1102 64-65 0.05097973894481811 1102 66-67 0.02480076797561992 1102 68-69 -0.07930348358375028 1102 70-71 -0.059726775603106574 1102 72-73 -0.09216776198065801 1102 74-75 -0.07395364563600282 1102 76-77 -0.11259852681124016 1102 78-79 -0.08114744038428867 1102 80-81 -0.15716658026985542 1102 82-83 0.03166512998140547 1102 84-85 -0.0030185287792328097 1102 86-87 0.09290356327825222 1102 88-89 0.21173152068900336 1102 90-91 0.12868336425660942 1102 92-93 0.10518566587967726 1102 94-95 0.21666207008742333 1102 96-97 0.4116406737864473 1102 98-99 0.45089467419163043 1102 100-101 0.8510254363558332 1103 1 0.08319151448014139 1103 2 0.15987333871553489 1103 3 0.1284386772062973 1103 4 0.07611304426445997 1103 5 0.006702659808148326 1103 6 0.03822465975310507 1103 7 0.14880725301851072 1103 8 0.004340304465372924 1103 9 0.12187362822458425 1103 10-11 0.01905321555525319 1103 12-13 0.19819556172525665 1103 14-15 0.15800927617999605 1103 16-17 0.08059102166563292 1103 18-19 0.2588072599546365 1103 20-21 0.1997380996987843 1103 22-23 0.2611795602140816 1103 24-25 0.22275645901063257 1103 26-27 0.19067719108778647 1103 28-29 0.33832000482855307 1103 30-31 0.15625723579982065 1103 32-33 0.10496297482244188 1103 34-35 0.3189259032124383 1103 36-37 0.3373424869864081 1103 38-39 0.15136467520520824 1103 40-41 0.30835753995825854 1103 42-43 0.34168547426005347 1103 44-45 0.42929235880591676 1103 46-47 0.1360571809383444 1103 48-49 0.11789849753799331 1103 50-51 0.01952417600632117 1103 52-53 -0.19349954101571853 1103 54-55 -0.12528313171866756 1103 56-57 -0.07312044795476424 1103 58-59 -0.30942239616355494 1103 60-61 -0.197246250033011 1103 62-63 -0.3686259088995385 1103 64-65 -0.13285318793762713 1103 66-67 -0.05797516297580785 1103 68-69 -0.05647458542904715 1103 70-71 -0.2038468793235566 1103 72-73 0.03296767505747411 1103 74-75 0.29277924401174715 1103 76-77 0.09075033216316086 1103 78-79 0.13826391586460218 1103 80-81 0.30686587094765017 1103 82-83 0.48529157198751705 1103 84-85 0.2487677306091136 1103 86-87 0.39249029162258253 1103 88-89 0.3136107829722654 1103 90-91 0.5672972605160318 1103 92-93 0.6898837125672976 1103 94-95 0.6585830620735749 1103 96-97 0.5803285530520839 1103 98-99 0.7467705115531338 1103 100-101 1.2038309538007965 1104 1 0.16790030613756812 1104 2 0.16091246588759134 1104 3 0.13841585334661488 1104 4 0.1636194112270175 1104 5 0.07782308968990748 1104 6 0.12636189063957914 1104 7 0.18599517464751614 1104 8 0.2133010995288842 1104 9 0.1184656021579471 1104 10-11 0.18313713114974917 1104 12-13 0.08520190341438649 1104 14-15 0.22123758865301824 1104 16-17 0.2709111972577105 1104 18-19 0.20881591124727095 1104 20-21 0.111137198409061 1104 22-23 0.11828159103212954 1104 24-25 0.07154012614778082 1104 26-27 0.03890873078458412 1104 28-29 0.1656445779990463 1104 30-31 0.26315319088308087 1104 32-33 0.13642646247928525 1104 34-35 0.25916782834861607 1104 36-37 0.2547537480532611 1104 38-39 0.35286591749193974 1104 40-41 0.4431145455611798 1104 42-43 0.4948080076695973 1104 44-45 0.546001212674895 1104 46-47 0.5888313297094712 1104 48-49 0.502423667939297 1104 50-51 0.46111093972299955 1104 52-53 0.4558966181991764 1104 54-55 0.4377867357318692 1104 56-57 0.16896750750177603 1104 58-59 0.04714986448725966 1104 60-61 0.08805831435009992 1104 62-63 -0.010617922492755838 1104 64-65 0.2729750615462123 1104 66-67 0.3699828986680487 1104 68-69 0.4532576327623872 1104 70-71 0.4078617218110274 1104 72-73 0.47813014438912305 1104 74-75 0.653908986183783 1104 76-77 0.44059353963476866 1104 78-79 0.5215103474494036 1104 80-81 0.5181216886989368 1104 82-83 0.35235960030932034 1104 84-85 0.28963779791422795 1104 86-87 0.5153367029886304 1104 88-89 0.3883409404063478 1104 90-91 0.6082256511423054 1104 92-93 0.7139080606097714 1104 94-95 0.6294179992982407 1104 96-97 0.8227022927823207 1104 98-99 0.9984871613060378 1104 100-101 1.2444842527622555 1105 1 0.09059533369207884 1105 2 0.17294874939241822 1105 3 0.11980959477647701 1105 4 0.1100191169159146 1105 5 0.04019437503417578 1105 6 -0.027423091319001003 1105 7 0.046855142763817526 1105 8 0.10345839989342664 1105 9 0.11390229368497984 1105 10-11 0.24879600526114842 1105 12-13 0.2390519162347573 1105 14-15 0.14966276414345003 1105 16-17 0.3573669409692357 1105 18-19 0.3044168521509363 1105 20-21 0.2860479203259487 1105 22-23 0.2834432577545343 1105 24-25 0.17390799272673974 1105 26-27 0.08226573535161208 1105 28-29 0.11085143188027757 1105 30-31 -0.11785148019081504 1105 32-33 0.026292052978085678 1105 34-35 0.11487921023584846 1105 36-37 0.11158180273864104 1105 38-39 0.21237138565840752 1105 40-41 0.2038195544239585 1105 42-43 0.152658347502296 1105 44-45 0.21360803980056886 1105 46-47 0.23506901565128402 1105 48-49 0.18513066117237287 1105 50-51 0.0117588329088818 1105 52-53 -0.007849605365777279 1105 54-55 0.01183830706329303 1105 56-57 0.1889784326865538 1105 58-59 -0.1541689613898214 1105 60-61 -0.055449933421193975 1105 62-63 -0.046464791535463235 1105 64-65 -0.14633464514601968 1105 66-67 -0.0959595100007462 1105 68-69 -0.244735329040342 1105 70-71 -0.08373071677197075 1105 72-73 -0.2523856804065865 1105 74-75 -0.23474703028574595 1105 76-77 -0.02358560366724305 1105 78-79 -0.10065291345813066 1105 80-81 -0.022054377714294304 1105 82-83 -0.10749777772065272 1105 84-85 0.15699527813555747 1105 86-87 0.23391843563375048 1105 88-89 0.0671126374888793 1105 90-91 0.16864861186738267 1105 92-93 0.3813773939133789 1105 94-95 0.36377250296412456 1105 96-97 0.36308831597790103 1105 98-99 0.47303222397720646 1105 100-101 0.6584985892802422 1106 1 -0.20756245530378692 1106 2 -0.19358614277533093 1106 3 -0.20072704853575374 1106 4 -0.039554589948110674 1106 5 -0.14614985645378198 1106 6 -0.22710093688490218 1106 7 -0.07977822057409867 1106 8 -0.13420419071250222 1106 9 -0.19241814469771867 1106 10-11 -0.3058535640590847 1106 12-13 -0.2983344918661288 1106 14-15 -0.18833268428464578 1106 16-17 -0.16601820729024297 1106 18-19 -0.18469113450667152 1106 20-21 -0.2842891311187472 1106 22-23 -0.3096569171844479 1106 24-25 -0.30671536557531454 1106 26-27 -0.12195052221753144 1106 28-29 -0.1885135589165685 1106 30-31 -0.10635849792861762 1106 32-33 -0.17666173119598483 1106 34-35 -0.5121235077228548 1106 36-37 -0.3030402536103125 1106 38-39 -0.20722950079268543 1106 40-41 -0.12145728607625728 1106 42-43 -0.47034463716378383 1106 44-45 -0.839898372581672 1106 46-47 -0.7610637274165128 1106 48-49 -0.705137678030276 1106 50-51 -0.3747615658186447 1106 52-53 -0.6528805135921232 1106 54-55 -0.739820279422716 1106 56-57 -0.7075540205124398 1106 58-59 -0.90289201210107 1106 60-61 -1.1534719122144494 1106 62-63 -1.3178132254192363 1106 64-65 -1.4517233623712826 1106 66-67 -1.4425457433381155 1106 68-69 -1.156797345523561 1106 70-71 -1.113330848721933 1106 72-73 -1.3038759955950425 1106 74-75 -1.1841270233724117 1106 76-77 -0.9769074264717759 1106 78-79 -0.7163012668032884 1106 80-81 -0.9129263178174334 1106 82-83 -1.1437594527482453 1106 84-85 -1.1278990829335918 1106 86-87 -0.8825993486596424 1106 88-89 -1.0188259180450352 1106 90-91 -1.2692971798587251 1106 92-93 -1.1077867416652083 1106 94-95 -1.0931305682063872 1106 96-97 -1.463385198400612 1106 98-99 -1.6399095759115454 1106 100-101 -1.068835231846844 1107 1 0.07298702427564763 1107 2 0.08247451131670402 1107 3 0.16949115825877925 1107 4 0.11830666645808208 1107 5 0.06037204347752834 1107 6 0.05947591100435545 1107 7 -0.05481494060368419 1107 8 0.10534988547495061 1107 9 0.1316691380200936 1107 10-11 0.05657788307992462 1107 12-13 0.1856830492127557 1107 14-15 0.17007276824349304 1107 16-17 0.19578979186440648 1107 18-19 0.16757388372126059 1107 20-21 0.22297562293631046 1107 22-23 0.21211621115073598 1107 24-25 -0.002117700863522032 1107 26-27 0.053264778675377045 1107 28-29 0.23942325093179306 1107 30-31 0.1797650293076103 1107 32-33 -0.005947936088467998 1107 34-35 0.24994549423202272 1107 36-37 0.3384507380946573 1107 38-39 0.36021066405118773 1107 40-41 0.17541173701244617 1107 42-43 0.33444323201780435 1107 44-45 -0.2052822157686549 1107 46-47 -0.44241629253512826 1107 48-49 -0.2579881033486089 1107 50-51 -0.059685748203605726 1107 52-53 0.029187431671253705 1107 54-55 -0.02945730589284068 1107 56-57 0.10117742134310959 1107 58-59 -0.029300846042005446 1107 60-61 -0.12839239617915865 1107 62-63 -0.1422280825017097 1107 64-65 -0.2077723928568318 1107 66-67 -0.11162166662231243 1107 68-69 -0.16451119346565335 1107 70-71 0.17525793978126103 1107 72-73 0.16281180490160096 1107 74-75 0.33063495686745625 1107 76-77 0.2919508183636452 1107 78-79 0.1805032981039858 1107 80-81 0.48050951459129365 1107 82-83 0.3156783873743336 1107 84-85 0.3633694752108596 1107 86-87 0.558075877208168 1107 88-89 0.47940729376877655 1107 90-91 0.4012412044599749 1107 92-93 0.29991231259590023 1107 94-95 0.2951760836666004 1107 96-97 0.27436616208969156 1107 98-99 0.263828998611622 1107 100-101 0.519149129118972 1108 1 -0.030605220006250988 1108 2 0.07591465992926771 1108 3 0.09283029677033028 1108 4 0.1616179401141835 1108 5 -0.10965698241356847 1108 6 0.063451297048708 1108 7 0.042814224611689156 1108 8 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98-99 -0.014598371566179935 2316 100-101 0.49937507430158945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 8.0 5 20.0 6 57.0 7 157.0 8 346.0 9 632.0 10 1016.0 11 1777.0 12 2784.0 13 4134.0 14 5104.0 15 7637.0 16 11212.0 17 17141.0 18 15673.0 19 8056.0 20 5818.0 21 5618.0 22 5564.0 23 5670.0 24 6386.0 25 7219.0 26 8293.0 27 9836.0 28 11535.0 29 14255.0 30 17603.0 31 21657.0 32 27248.0 33 36165.0 34 49945.0 35 74499.0 36 111429.0 37 172997.0 38 146900.0 39 13518.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01364419704686 20.362362802704187 15.5971662610066 19.026826739242356 2 15.509265410342572 22.38893223725055 40.22306556929551 21.87873678311137 3 19.358318094304025 28.037559275193917 31.417845728683275 21.18627690181878 4 11.891274256629279 18.664167224290328 37.902425119395886 31.54213339968451 5 11.730778057742528 40.10160868336309 33.34086258669714 14.826750672197244 6 28.288429530460746 38.52241405549825 17.558019030870206 15.631137383170799 7 25.873214497087876 37.09316623143838 20.201879896318236 16.83173937515551 8 27.230353730683472 34.356300986329565 19.082566147855225 19.33077913513174 9 27.855777987675108 16.50791503506386 20.829357496756927 34.80694948050411 10-11 24.558289589065584 28.653683094219474 25.842776700107983 20.94525061660696 12-13 26.75302838775684 26.94079839395947 27.354327256123966 18.951845962159723 14-15 23.153259139190112 26.873615502984592 28.42286313378861 21.55026222403668 16-17 19.399203785882175 30.71548148488135 28.660628226466873 21.2246865027696 18-19 19.31586219891342 27.37783210414558 34.157790988962674 19.148514707978325 20-21 21.06374382409799 25.825763619596444 34.43286180775695 18.677630748548612 22-23 20.376128975761183 26.531768343685734 34.296041526378254 18.796061154174833 24-25 19.887657956464288 26.71556845001558 34.763044166209696 18.633729427310435 26-27 19.80398752757709 26.73768112703548 34.89615071267106 18.562180632716377 28-29 19.111532252658357 27.018746636856612 35.06540879819448 18.80431231229055 30-31 19.313808855466363 26.84257378146145 34.93504187612783 18.90857548694436 32-33 19.12514282815448 27.25149108969729 34.579149142789504 19.04421693935873 34-35 19.626460591507854 26.853504815694308 34.68930497947865 18.830729613319193 36-37 20.00862409456066 26.609800788247075 34.477475290278086 18.90409982691418 38-39 19.72097478252677 27.052920699876076 34.445561033822194 18.780543483774963 40-41 19.38902088011238 27.43511728832721 34.46891415443819 18.706947677122223 42-43 19.479629084859734 27.667440060937267 33.673638893536776 19.179291960666227 44-45 19.480431636949554 27.821656250981373 33.16922690411369 19.528685207955377 46-47 19.884698726202355 28.325087750236133 32.26376283641616 19.526450687145346 48-49 20.220541164704716 28.72512736768617 31.414342966332416 19.639988501276697 50-51 20.24119538408393 29.02654610722318 31.057483953724883 19.674774554968007 52-53 20.33516604301392 29.666766515524483 30.47203950149652 19.52602793996507 54-55 19.743138813264117 29.657828432284354 30.492331366149788 20.10670138830174 56-57 20.09830683715054 29.89873393258753 30.108658103821877 19.89430112644006 58-59 20.155981631031093 29.42012373206042 30.527902521747325 19.895992115161164 60-61 20.085020497199 29.236711848274826 31.020886126403823 19.65738152812235 62-63 20.08411461038412 29.21714469307347 31.170599020675958 19.52814167586645 64-65 19.93367700666008 29.30121098949413 31.051082353566418 19.714029650279375 66-67 19.722544986339226 29.496339009418808 30.762587599255 20.018528404986967 68-69 19.758961636297315 29.749202215678366 30.54976459021304 19.942071557811282 70-71 19.919726349710963 30.007319565464215 30.27582441739399 19.79712966743083 72-73 19.987909430644095 29.790087907256517 30.012815278807803 20.20918738329158 74-75 19.843873427078528 30.38151121246307 29.55950951664295 20.21510584381545 76-77 19.830103947492383 29.831335953560618 30.17412352431038 20.164436574636618 78-79 19.937240161465265 29.957012651011333 29.575513517039127 20.530233670484275 80-81 19.870083752255656 30.37994100865061 29.60897093673528 20.141004302358446 82-83 19.794303176343053 30.34438817877385 29.51858125960618 20.342727385276916 84-85 19.55461718988739 30.33776332651703 29.30927858710475 20.79834089649083 86-87 19.558156725666066 29.941491790249756 29.5342050782807 20.966146405803475 88-89 19.555016318041158 29.863162776989995 29.53680195381668 21.045018951152166 90-91 19.403772071498548 29.68226222818701 29.6939797371654 21.21998596314904 92-93 19.51100197246759 30.051322809479924 29.511883724666234 20.925791493386253 94-95 19.131785998130248 29.95791853782621 29.565065622440866 21.34522984160267 96-97 19.289591481281963 29.733983317188418 29.479549907116404 21.496875294413215 98-99 19.145127567751686 30.311699417692438 28.957994378636403 21.585178635919476 100-101 18.75229524039623 30.50664411693646 29.353587823145688 21.387472819521626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1730.0 1 1167.5 2 727.5 3 863.5 4 764.0 5 782.0 6 1415.0 7 2861.5 8 4338.5 9 4927.5 10 4400.5 11 3695.5 12 3716.0 13 3869.5 14 4030.5 15 4088.0 16 4051.0 17 4099.0 18 4081.0 19 4158.0 20 4387.0 21 4734.5 22 5178.5 23 5719.5 24 6394.5 25 7361.0 26 8542.5 27 9816.5 28 10605.0 29 11466.0 30 13139.5 31 14348.5 32 15726.0 33 17691.5 34 20537.0 35 22399.0 36 23659.5 37 25439.0 38 26082.0 39 25889.5 40 25458.5 41 26084.0 42 26483.0 43 27178.0 44 28516.5 45 28749.0 46 27418.0 47 27254.5 48 32148.5 49 31248.0 50 25436.0 51 22792.5 52 20507.5 53 19265.5 54 17878.5 55 16218.5 56 14279.5 57 12610.0 58 11730.0 59 10483.5 60 9146.5 61 7722.5 62 6084.0 63 4488.5 64 2966.0 65 2126.5 66 1520.0 67 1132.0 68 783.0 69 464.0 70 348.0 71 281.5 72 249.5 73 224.5 74 182.5 75 143.0 76 97.0 77 56.0 78 47.0 79 32.0 80 20.0 81 15.0 82 11.5 83 9.0 84 5.5 85 2.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05556105797917185 2 0.0 3 0.0 4 0.0 5 0.004348256711413449 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005254143526291251 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.0196227162593395E-4 22-23 0.0012682415408289227 24-25 0.0 26-27 0.0013890264494792963 28-29 0.0011474566321785491 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.039245432518679E-4 38-39 0.0 40-41 1.2078490865037359E-4 42-43 0.021922460920042807 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.039245432518679E-5 84-85 1.2078490865037359E-4 86-87 0.0 88-89 0.0 90-91 0.011716136139086238 92-93 0.0025364830816578454 94-95 0.0 96-97 0.0 98-99 6.039245432518679E-4 100-101 0.014252619220744082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 827918.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.905573571377545 #Duplication Level Percentage of deduplicated Percentage of total 1 75.67120572290179 37.00743718716218 2 12.92320079424593 12.64033094441358 3 4.493403117691949 6.592573702744225 4 2.0932733752095087 4.094909402252577 5 1.1998020417574955 2.93385035121301 6 0.7358769925708842 2.1593091839795653 7 0.4973811913155773 1.7027298711432362 8 0.3672989567993106 1.4370372923551125 9 0.26668760442752826 1.1738259233014423 >10 1.5529065293480655 14.14892022160755 >50 0.12438369130742459 4.131502449093215 >100 0.06937672783777084 6.068701852373196 >500 0.002973288335277645 1.0279301506994405 >1k 0.0014866441676388226 1.3371695406068993 >5k 2.4777402793980377E-4 0.873552806364761 >10k+ 4.955480558796075E-4 2.670219120689953 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11741 1.4181356124640363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10295 1.243480634555596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7209 0.8707384064605432 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2278 0.27514802190555104 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2147 0.2593251988723521 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2098 0.25340673834848376 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2009 0.2426568814786005 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1257 0.1518266301735196 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1246 0.15049799617836548 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 982 0.11861078029466686 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 941 0.11365859904000156 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 895 0.10810249324208435 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 858 0.10363345162202053 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.2078490865037359E-4 0.0 20-21 0.0 0.0 0.0 1.811773629755604E-4 0.0 22-23 0.0 0.0 0.0 3.623547259511208E-4 0.0 24-25 0.0 0.0 0.0 6.643169975770547E-4 0.0 26-27 0.0 0.0 0.0 0.0016305962667800434 0.0 28-29 0.0 0.0 0.0 0.0034423698965356476 0.0 30-31 0.0 0.0 0.0 0.00942122287472914 0.0 32-33 0.0 0.0 0.0 0.015702038124548567 0.0 34-35 0.0 0.0 0.0 0.02041264956191314 0.0 36-37 0.0 0.0 0.0 0.027478566717959993 0.0 38-39 0.0 0.0 0.0 0.037020574501339505 0.0 40-41 0.0 0.0 0.0 0.04505277092658935 0.0 42-43 0.0 0.0 0.0 0.06238540531791796 0.0 44-45 0.0 0.0 0.0 0.08334158696875778 0.0 46-47 0.0 0.0 0.0 0.10810249324208436 0.0 48-49 0.0 0.0 0.0 0.13618498450329622 0.0 50-51 0.0 0.0 0.0 0.1719373174638068 0.0 52-53 0.0 0.0 0.0 0.24024118330559308 0.0 54-55 0.0 0.0 0.0 0.2919371242079529 0.0 56-57 0.0 0.0 0.0 0.38808191149365034 0.0 58-59 0.0 0.0 0.0 0.5185296128360538 0.0 60-61 0.0 0.0 0.0 0.647588287728978 0.0 62-63 0.0 0.0 0.0 0.8285844733415628 0.0 64-65 0.0 0.0 0.0 0.9410956157493857 0.0 66-67 0.0 0.0 0.0 1.137431484760568 0.0 68-69 0.0 0.0 0.0 1.3678286980111558 0.0 70-71 0.0 0.0 0.0 1.544416234458002 0.0 72-73 0.0 0.0 0.0 1.8588193516749243 0.0 74-75 0.0 0.0 0.0 2.163499283745492 0.0 76-77 0.0 0.0 0.0 2.370886971898183 0.0 78-79 0.0 0.0 0.0 2.606538328675062 0.0 80-81 0.0 0.0 0.0 3.00029713087528 0.0 82-83 0.0 0.0 0.0 3.35588790194198 0.0 84-85 0.0 0.0 0.0 3.8032148111286386 0.0 86-87 0.0 0.0 0.0 4.372292908234874 0.0 88-89 0.0 0.0 0.0 5.127862904297285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 215 0.0 33.137344 4 GGTATCA 8000 0.0 31.610231 1 GTATCAA 11375 0.0 27.622059 1 CGTATAG 70 0.008360849 27.162388 1 AGGTAAG 215 5.820766E-11 26.509874 2 TATAACG 90 8.9488784E-4 26.387146 2 GTACATG 7740 0.0 25.241018 1 TACATGG 7770 0.0 24.75705 2 GAGTACT 7880 0.0 24.082851 12-13 GTATAGA 340 0.0 23.767088 1 CGCACGT 50 0.0016545891 23.74843 56-57 TGGGACG 100 0.0016538927 23.74843 6 AGTACTT 7920 0.0 23.481396 12-13 TAAGGTG 325 0.0 23.383068 5 ACATGGG 7820 0.0 23.323265 3 GTCGGGA 185 2.5414738E-7 23.10658 2 GTACTTT 8335 0.0 22.623661 14-15 CATGGGG 1805 0.0 22.366941 4 TATCAAC 14160 0.0 22.238995 2 ATCAACG 14245 0.0 22.106295 3 >>END_MODULE