FastQCFastQC Report
Thu 26 May 2016
SRR937784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612309
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118741.939216963983871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97421.5910267528323117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66471.085563008219706No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.34165756178661427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.31079079353725No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.17785137896062284No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.16298960165537335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9800.16004990944114816No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.14894440552074198No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.13473589315198697No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8230.13440926068373973No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8150.13310273081075077No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7720.12608013274343507No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA6440.10517565477561165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49350.044.7067831
GTATCAA78100.034.398541
GTATAGA1900.032.533621
TATCAAC97800.027.4119932
ATCAACG98950.027.1414493
TCAACGC99400.026.9751684
CAACGCA100350.026.5776735
GTACATG47400.026.1821161
AACGCAG102100.026.1093046
GAGTACT68500.025.6866312-13
CATGGGG11500.025.6309874
TACATGG48100.025.1997072
ATGGGCG950.001219694425.021765
GTGTAGC2151.6389095E-924.3274611
GTACTTT72500.024.16920314-15
ACATGGG48200.024.1612513
ACGCAGA111450.023.663077
AGTACTT69050.023.62503812-13
CGCAGAG111400.023.588388
GCAGAGT113050.022.7780119
Produced by FastQC (version 0.11.5)