##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937784_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 612309 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.939314953724345 33.0 31.0 34.0 30.0 34.0 2 31.99023695552409 33.0 31.0 34.0 30.0 34.0 3 31.978840748706943 33.0 31.0 34.0 30.0 34.0 4 35.62783333251675 37.0 35.0 37.0 33.0 37.0 5 35.653165313591664 37.0 35.0 37.0 35.0 37.0 6 35.76099812349647 37.0 35.0 37.0 35.0 37.0 7 35.69482891807895 37.0 35.0 37.0 35.0 37.0 8 35.70396972770284 37.0 35.0 37.0 35.0 37.0 9 37.25715774225105 39.0 37.0 39.0 34.0 39.0 10-11 37.21559784357244 39.0 37.0 39.0 34.0 39.0 12-13 37.118229521369116 39.0 37.0 39.0 34.0 39.0 14-15 38.145320418285536 40.0 38.0 41.0 33.5 41.0 16-17 38.10817414083412 40.0 38.0 41.0 33.5 41.0 18-19 38.20825759542976 40.0 38.0 41.0 33.5 41.0 20-21 38.3066262295671 40.0 38.0 41.0 34.0 41.0 22-23 38.28251830366693 40.0 38.0 41.0 34.0 41.0 24-25 38.195193113281036 40.0 38.0 41.0 34.0 41.0 26-27 38.067901174080404 40.0 38.0 41.0 34.0 41.0 28-29 37.90000228642728 40.0 38.0 41.0 33.0 41.0 30-31 37.52508537356139 39.5 37.0 41.0 33.0 41.0 32-33 37.19573124027248 39.0 35.5 41.0 33.0 41.0 34-35 37.06030370286898 39.0 35.0 41.0 33.0 41.0 36-37 36.839884764065204 39.0 35.0 41.0 32.0 41.0 38-39 36.62821140959875 39.0 35.0 41.0 31.0 41.0 40-41 36.325530083666905 39.0 35.0 41.0 30.5 41.0 42-43 35.92573030936994 39.0 35.0 41.0 30.0 41.0 44-45 35.42548860134344 39.0 35.0 41.0 27.5 41.0 46-47 34.6904806233454 39.0 35.0 40.5 22.5 41.0 48-49 34.03041764860552 38.5 34.0 40.0 15.5 41.0 50-51 33.02710722853984 37.5 33.0 39.5 8.0 40.5 52-53 33.10200650325244 38.0 33.5 39.5 6.0 40.5 54-55 33.3477361920207 38.0 34.0 40.0 2.0 41.0 56-57 33.12851762753773 38.0 33.5 40.0 2.0 41.0 58-59 32.86948419833777 38.0 33.0 40.0 2.0 41.0 60-61 32.536895587032035 37.5 33.0 40.0 2.0 41.0 62-63 32.19335662222832 37.0 32.5 40.0 2.0 41.0 64-65 31.766980397152416 36.5 31.5 39.5 2.0 41.0 66-67 31.24553044296262 36.0 31.0 39.0 2.0 41.0 68-69 30.810214287230792 35.0 31.0 39.0 2.0 40.0 70-71 30.35557537125863 35.0 30.0 38.5 2.0 40.0 72-73 29.888887800113995 35.0 29.5 37.5 2.0 40.0 74-75 29.371768175871985 35.0 29.0 37.0 2.0 39.0 76-77 28.855492079979225 34.0 28.5 36.5 2.0 39.0 78-79 28.37388802059091 34.0 27.5 36.0 2.0 39.0 80-81 27.874502089631214 34.0 26.0 35.5 2.0 37.5 82-83 27.37923907700197 34.0 26.0 35.0 2.0 37.0 84-85 26.885082531858913 33.5 25.0 35.0 2.0 36.5 86-87 26.353146858857208 33.0 24.0 35.0 2.0 36.0 88-89 25.84750673271175 33.0 21.5 35.0 2.0 36.0 90-91 25.336533514940985 32.0 19.0 35.0 2.0 35.0 92-93 24.761139392038984 32.0 15.0 34.5 2.0 35.0 94-95 24.088753390853313 31.5 4.5 34.0 2.0 35.0 96-97 23.318652837047964 31.0 2.0 34.0 2.0 35.0 98-99 22.270922851044162 31.0 2.0 34.0 2.0 35.0 100-101 19.281998958042426 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19145599220108522 1101 2 0.26045959115467454 1101 3 0.13136044504254585 1101 4 0.08142700296953365 1101 5 -0.023382474104316486 1101 6 -0.0037056422592272042 1101 7 -0.012818470491346545 1101 8 0.03202119142282811 1101 9 0.292800359519255 1101 10-11 0.2647100407221359 1101 12-13 0.2976663995666158 1101 14-15 0.5066007433347437 1101 16-17 0.5108043136648845 1101 18-19 0.4926236283250631 1101 20-21 0.3494155852080283 1101 22-23 0.3070426259679593 1101 24-25 0.188201543807601 1101 26-27 0.3782337480786282 1101 28-29 0.4387644319297337 1101 30-31 0.710313732035317 1101 32-33 0.5322511478143284 1101 34-35 0.515165006509605 1101 36-37 0.4341558801046972 1101 38-39 0.5099927116374019 1101 40-41 0.6314479202774379 1101 42-43 0.7538476926761675 1101 44-45 0.998924184195964 1101 46-47 1.1380508290297087 1101 48-49 1.509995322925171 1101 50-51 1.6238743732324963 1101 52-53 1.702669625332014 1101 54-55 1.6721098824966987 1101 56-57 1.7053015156855693 1101 58-59 1.8080484479968675 1101 60-61 1.6885944432908069 1101 62-63 1.6254544386783039 1101 64-65 1.954299425682187 1101 66-67 2.0886544152233206 1101 68-69 1.917821487754079 1101 70-71 1.924960692522621 1101 72-73 2.0723701610163836 1101 74-75 1.9695060967349711 1101 76-77 2.110943782613912 1101 78-79 2.1014733908628216 1101 80-81 2.176444184652194 1101 82-83 2.054774121759312 1101 84-85 2.2587105682302635 1101 86-87 2.328967135616111 1101 88-89 2.2979289225496586 1101 90-91 2.5825572218981883 1101 92-93 2.7664818737131647 1101 94-95 2.8001208597368965 1101 96-97 2.6956194368301745 1101 98-99 2.865557317951943 1101 100-101 3.500182705913364 1102 1 0.16546815025579065 1102 2 0.2671248104338808 1102 3 0.22504259875509547 1102 4 0.2668807589400046 1102 5 0.14759017027258636 1102 6 0.12490920793617022 1102 7 0.1513152681104728 1102 8 0.2124163282009448 1102 9 0.34421156229824135 1102 10-11 0.3867465148862692 1102 12-13 0.2334023960928846 1102 14-15 0.4691061710377369 1102 16-17 0.42716341049510476 1102 18-19 0.28388502650135194 1102 20-21 0.3758267879870161 1102 22-23 0.2683213927938368 1102 24-25 0.11524236013413258 1102 26-27 0.21525068251197155 1102 28-29 0.2852472803882691 1102 30-31 0.3885812092389642 1102 32-33 0.444040118721837 1102 34-35 0.33298958314876614 1102 36-37 0.3612292626492035 1102 38-39 0.5242132934871648 1102 40-41 0.9387536085101829 1102 42-43 1.1512966722680034 1102 44-45 1.336212503779116 1102 46-47 1.291513703541213 1102 48-49 1.2796653185830067 1102 50-51 0.9667206606836487 1102 52-53 0.9451251181674465 1102 54-55 1.2135775333868466 1102 56-57 1.5079936563716245 1102 58-59 1.4435347701853232 1102 60-61 1.4393000446802908 1102 62-63 1.499586006198932 1102 64-65 1.4460601725341178 1102 66-67 1.7004759523488069 1102 68-69 1.4047298681275038 1102 70-71 1.5841117997740355 1102 72-73 1.7213606082591077 1102 74-75 1.575639401120558 1102 76-77 1.6659915464002744 1102 78-79 1.987328796855003 1102 80-81 2.036431158165005 1102 82-83 2.231413288064136 1102 84-85 2.233591158764348 1102 86-87 2.2645837226764627 1102 88-89 2.0452259264576043 1102 90-91 1.932177282688464 1102 92-93 2.1196082306389137 1102 94-95 2.209304533206282 1102 96-97 1.842015442040772 1102 98-99 2.207524317517727 1102 100-101 2.6418457541113654 1103 1 -0.0050803021310343865 1103 2 -0.04182271387870529 1103 3 0.18487623650994323 1103 4 0.06479506016921022 1103 5 0.12737987259770733 1103 6 0.09519108580554558 1103 7 0.10962103895511888 1103 8 0.060194873888853806 1103 9 0.18370901996718914 1103 10-11 0.22556519484874826 1103 12-13 0.09992384546840327 1103 14-15 0.2527463941704795 1103 16-17 0.2962901321221949 1103 18-19 0.34017914705227525 1103 20-21 0.2788973315028329 1103 22-23 -0.07503275638916307 1103 24-25 -0.02866715262688757 1103 26-27 -0.07292871226513853 1103 28-29 0.06057062540480018 1103 30-31 0.07798857459414421 1103 32-33 0.03873701362236659 1103 34-35 0.09756355757736657 1103 36-37 0.3038478636266504 1103 38-39 0.4830596182144191 1103 40-41 0.46298350434865654 1103 42-43 0.26272358642113147 1103 44-45 0.3888609709756352 1103 46-47 0.38118023856614514 1103 48-49 0.3302330235738751 1103 50-51 0.46991585246919954 1103 52-53 0.526224244395415 1103 54-55 0.5905435204530463 1103 56-57 0.5837995506303955 1103 58-59 0.5395781132272361 1103 60-61 0.6878770797792733 1103 62-63 0.82880923767447 1103 64-65 0.6871040092325451 1103 66-67 0.8479422602917417 1103 68-69 0.8772726928300791 1103 70-71 0.885750739399839 1103 72-73 1.1150911705338764 1103 74-75 1.206376923952174 1103 76-77 1.1374779461633189 1103 78-79 1.0344223941300186 1103 80-81 1.1579916064051154 1103 82-83 1.5423848040832873 1103 84-85 1.6200723751339972 1103 86-87 1.4979228153357909 1103 88-89 1.5816639478822978 1103 90-91 1.6198210601481051 1103 92-93 1.7256690984870637 1103 94-95 1.9801281990995534 1103 96-97 1.971612618325512 1103 98-99 2.4213861924251674 1103 100-101 2.4459850639795597 1104 1 0.2126595917511409 1104 2 0.27117789422393557 1104 3 0.2690593041137319 1104 4 0.26353370391763065 1104 5 0.23077950564821492 1104 6 0.263721643615753 1104 7 0.3300386723657951 1104 8 0.21312355362755397 1104 9 0.37181369713776746 1104 10-11 0.3089170148503726 1104 12-13 0.368753495822439 1104 14-15 0.6455128436079249 1104 16-17 0.5018014211692972 1104 18-19 0.2980510748942393 1104 20-21 0.2511229610717578 1104 22-23 0.2069944177082803 1104 24-25 0.2238194130025235 1104 26-27 0.3022334266902291 1104 28-29 0.26846473724871345 1104 30-31 0.5114104699430726 1104 32-33 0.5761327161897114 1104 34-35 0.4466249133069695 1104 36-37 0.3799898829971937 1104 38-39 0.5507527951983846 1104 40-41 0.782163009462721 1104 42-43 0.8869265935278392 1104 44-45 0.8479158280976193 1104 46-47 1.0894488685604813 1104 48-49 1.2204163417263985 1104 50-51 1.1444191265668948 1104 52-53 1.1386410217645988 1104 54-55 1.4584749797166978 1104 56-57 1.4362994266609732 1104 58-59 1.5135321375356767 1104 60-61 1.6868187723925239 1104 62-63 1.7995746379391164 1104 64-65 1.8800506710622287 1104 66-67 2.0251480678024585 1104 68-69 1.8836016580899262 1104 70-71 1.8032308194710396 1104 72-73 1.9760862143668554 1104 74-75 2.014673218701869 1104 76-77 2.1598550794160936 1104 78-79 2.1359664006651684 1104 80-81 2.253955031510621 1104 82-83 2.4796817225949255 1104 84-85 2.5190673093519074 1104 86-87 2.6482624915537585 1104 88-89 2.5626163723327195 1104 90-91 2.506038661718211 1104 92-93 2.6091260967567145 1104 94-95 2.775980573701222 1104 96-97 2.850392054538677 1104 98-99 3.123005494072789 1104 100-101 3.5248974737102436 1105 1 0.17745763423720717 1105 2 0.012734984070466027 1105 3 0.09333557992408359 1105 4 0.04682695605421827 1105 5 0.008760383038541875 1105 6 -0.01063738047175633 1105 7 0.1633720056991308 1105 8 0.05049409782677117 1105 9 0.2372233011096867 1105 10-11 0.27587588637074134 1105 12-13 0.17424979154409215 1105 14-15 0.3298208934637614 1105 16-17 0.31573335424663185 1105 18-19 0.2922336447454157 1105 20-21 0.2521718601317886 1105 22-23 0.16268126073220657 1105 24-25 0.25762741760539853 1105 26-27 0.42873808774553623 1105 28-29 0.1952680678668699 1105 30-31 0.3195912361404041 1105 32-33 0.4013310211429726 1105 34-35 0.3531840072133363 1105 36-37 0.42839116042372183 1105 38-39 0.30986076230595216 1105 40-41 0.43347114335978176 1105 42-43 0.48951506307259507 1105 44-45 0.765321087784983 1105 46-47 0.5010698297334386 1105 48-49 0.3968883560004741 1105 50-51 0.6655498365212509 1105 52-53 0.3614310609407383 1105 54-55 0.17405686818753452 1105 56-57 0.4393063245056439 1105 58-59 0.4596083823154231 1105 60-61 0.5684208566147539 1105 62-63 0.7422706479206198 1105 64-65 0.521054058569824 1105 66-67 0.7257908215099711 1105 68-69 0.5815090317371876 1105 70-71 0.8745648445282512 1105 72-73 1.0177900531111526 1105 74-75 1.1664653976967365 1105 76-77 0.9348500692665027 1105 78-79 1.2153368672878742 1105 80-81 1.391750307101173 1105 82-83 1.3642422193431791 1105 84-85 1.6438442768861385 1105 86-87 1.8202819373988888 1105 88-89 1.951391859448556 1105 90-91 1.8786984370049211 1105 92-93 2.1394205319350768 1105 94-95 2.1656263722867415 1105 96-97 2.232468486794989 1105 98-99 2.488528230454879 1105 100-101 3.26535394682352 1106 1 -0.5515861987685682 1106 2 -0.6128829064661332 1106 3 -0.49620643663240216 1106 4 -0.6052336716630151 1106 5 -0.6732344356143045 1106 6 -0.762424051902876 1106 7 -0.3720338664943057 1106 8 -0.48537333115304193 1106 9 -0.4070727503464511 1106 10-11 -0.7752973612023695 1106 12-13 -1.160393767505397 1106 14-15 -1.091680952763383 1106 16-17 -0.8051759126225235 1106 18-19 -0.622679850579452 1106 20-21 -0.7630222914970588 1106 22-23 -0.7342389643883891 1106 24-25 -1.1066488315757042 1106 26-27 -0.8689958173226628 1106 28-29 -0.5284529731670204 1106 30-31 -0.48596680090325606 1106 32-33 -0.3971212747298196 1106 34-35 -0.5143343061688412 1106 36-37 -0.8217577288622024 1106 38-39 -1.2605899255625488 1106 40-41 -1.1326580329846934 1106 42-43 -0.836656695607644 1106 44-45 -0.7855767146091566 1106 46-47 -0.8664484836487389 1106 48-49 -0.900436103531419 1106 50-51 -0.8385185632167023 1106 52-53 -0.851316839720333 1106 54-55 -0.5508255092765921 1106 56-57 -0.5071865883643483 1106 58-59 -1.0122143203228902 1106 60-61 -0.7735182774451594 1106 62-63 -0.9380752218905855 1106 64-65 -0.7489045502086853 1106 66-67 -0.8771698419406881 1106 68-69 -0.8547067981716907 1106 70-71 -0.9915799651912423 1106 72-73 -0.7831151937472853 1106 74-75 -0.7814270744609679 1106 76-77 -0.7463444694744901 1106 78-79 -0.48445766549754765 1106 80-81 -0.4178351851268083 1106 82-83 -0.6817385143832233 1106 84-85 -0.6498175062119493 1106 86-87 -0.7790718831001868 1106 88-89 -1.0064910837948489 1106 90-91 -0.6085789735126177 1106 92-93 -0.05582171093312738 1106 94-95 0.03793147621146531 1106 96-97 -0.0831581882094774 1106 98-99 -0.12520085060045005 1106 100-101 0.5606088452810276 1107 1 0.05137565688847445 1107 2 0.31599075227438433 1107 3 0.053485089521362994 1107 4 0.21788476455827777 1107 5 0.20527627545907734 1107 6 0.17604586714649173 1107 7 0.22001422248804658 1107 8 0.22238095390972745 1107 9 0.2956395392009057 1107 10-11 0.26072820348846193 1107 12-13 0.2678338063826402 1107 14-15 0.4663906739769246 1107 16-17 0.4228508682280321 1107 18-19 0.6502118225269484 1107 20-21 0.590335449486254 1107 22-23 0.3566309698629695 1107 24-25 0.5065015288382924 1107 26-27 0.48211329500742295 1107 28-29 0.5755128476774516 1107 30-31 0.15171261432661254 1107 32-33 0.39164064933620324 1107 34-35 0.17073733323980633 1107 36-37 0.7000096299175809 1107 38-39 0.7462858107841512 1107 40-41 0.7546603348276122 1107 42-43 0.6720179436616505 1107 44-45 0.6306254466080148 1107 46-47 0.30395322910953837 1107 48-49 0.10643013156745695 1107 50-51 0.150941385856612 1107 52-53 0.02657312316146232 1107 54-55 0.1924856122507066 1107 56-57 0.4576841313235107 1107 58-59 0.42164432644605654 1107 60-61 0.8447817592929958 1107 62-63 1.140116880672224 1107 64-65 0.8810820213610455 1107 66-67 1.0969424248036788 1107 68-69 1.1131884854088874 1107 70-71 0.9983265425301049 1107 72-73 0.9379345041130343 1107 74-75 0.6329223434292537 1107 76-77 0.7916795243433654 1107 78-79 0.9562735505354922 1107 80-81 1.0787918718933476 1107 82-83 1.2998417331559544 1107 84-85 1.1672929742978262 1107 86-87 1.1030714717270804 1107 88-89 0.9111742695882299 1107 90-91 1.007225402128718 1107 92-93 1.1292594297233443 1107 94-95 1.1855270270938156 1107 96-97 1.446083485530341 1107 98-99 1.5862399186205742 1107 100-101 2.413215987685728 1108 1 0.1511702779153694 1108 2 -0.08917306190654628 1108 3 0.06482983279764909 1108 4 0.009930404330077636 1108 5 0.3304270497052073 1108 6 0.3938453781489315 1108 7 0.08203867236579754 1108 8 0.19240214380378262 1108 9 0.2379704275464647 1108 10-11 0.08851956453165855 1108 12-13 0.22620381453260308 1108 14-15 0.34538985898100094 1108 16-17 0.3275322048213454 1108 18-19 0.5324807711822004 1108 20-21 0.35091898656856557 1108 22-23 0.4361582722264572 1108 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-2.525252329854947 2316 50-51 -2.5787089741560507 2316 52-53 -2.3955509852262615 2316 54-55 -2.7175314285731815 2316 56-57 -2.7541070411255895 2316 58-59 -2.835763043541892 2316 60-61 -2.9495092279297523 2316 62-63 -2.9697021363483955 2316 64-65 -3.1129941032996555 2316 66-67 -3.1014450956554107 2316 68-69 -3.044429032288651 2316 70-71 -2.78736994221061 2316 72-73 -2.7098626494631795 2316 74-75 -2.507236749163713 2316 76-77 -2.471005112646676 2316 78-79 -2.5554192835623226 2316 80-81 -2.865825450474585 2316 82-83 -3.057678793291 2316 84-85 -3.022264301056296 2316 86-87 -3.0539937346824253 2316 88-89 -3.0762572808573267 2316 90-91 -2.865780046893626 2316 92-93 -2.895210654537806 2316 94-95 -3.1132161279507393 2316 96-97 -3.0389704321752795 2316 98-99 -3.207788383965184 2316 100-101 -3.60665374767634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2355.0 3 396.0 4 359.0 5 398.0 6 455.0 7 567.0 8 702.0 9 886.0 10 1199.0 11 1703.0 12 2799.0 13 4192.0 14 4756.0 15 6837.0 16 9533.0 17 14528.0 18 12397.0 19 6216.0 20 5272.0 21 5192.0 22 5564.0 23 5662.0 24 6414.0 25 6940.0 26 7562.0 27 8815.0 28 10063.0 29 11889.0 30 14442.0 31 17701.0 32 22475.0 33 29291.0 34 40341.0 35 59582.0 36 80403.0 37 111536.0 38 84438.0 39 8449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.06332929958521 18.99551484326514 15.792041999014215 19.149113858135426 2 15.194019491977647 21.213812655708296 42.133968879620504 21.458198972693555 3 18.637173182821186 27.820629505706414 32.298344060815985 21.243853250656418 4 11.146905001595993 17.93044745091299 39.08201766232066 31.84062988517036 5 11.222331714950776 38.76588235679279 34.59530068849464 15.416485239761792 6 27.242071023731295 38.32382161603204 18.416789926306006 16.017317433930653 7 24.78449016319686 36.87872714630455 21.258456378583993 17.078326311914598 8 26.24001151824923 33.41519361784282 20.332982113991402 20.011812749916558 9 27.505419799242453 16.075819923591055 21.593134648265256 34.825625628901236 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25.43868619528234 37.128203785680455 18.65302946881522 36-37 18.888699829191836 25.256866684554623 37.09932069396543 18.75511279228811 38-39 18.705526184693685 25.630005668762664 37.04393250164214 18.62053564490151 40-41 18.59513596461092 25.886690159048005 37.01105254375374 18.507121332587335 42-43 18.641764843503314 26.050877988046995 36.33265546188241 18.97470170656728 44-45 18.692053603126794 26.36711368659742 35.798132627001486 19.1427000832743 46-47 19.032150134855343 26.755512217149413 34.89477029599459 19.317567352000662 48-49 19.359773681657202 27.12531363792174 33.99471018506171 19.520202495359346 50-51 19.28657032652844 27.48499883426524 33.589440488217896 19.63899035098842 52-53 19.43382766790141 28.032925823636432 33.02055655178733 19.512689956674826 54-55 19.086402382159978 27.82357945714239 33.157752654570444 19.932265506127187 56-57 19.32607870594548 28.139763908337805 32.692527908285456 19.841629477431262 58-59 19.534531083741385 27.562299569552483 33.06888714227178 19.834282204434352 60-61 19.418557379033807 27.297504889526557 33.63707974815768 19.64685798328195 62-63 19.4912717225404 27.292491767396438 33.69789085031793 19.518345659745226 64-65 19.424860595441714 27.27143337415063 33.78837664138285 19.51532938902481 66-67 19.279431227209745 27.53651757538677 33.279866378964826 19.904184818438655 68-69 19.206402570451424 27.53542100712551 33.421185447305504 19.836990975117565 70-71 19.314394813175163 27.732023866200073 32.97938129150368 19.974200029121082 72-73 19.379881106930956 27.62361926077535 32.780751331554924 20.215748300738767 74-75 19.204585056028847 27.89179699706971 32.66729711732436 20.236320829577082 76-77 19.198369876415562 27.739422356448017 32.82341527578275 20.238792491353667 78-79 19.182684206479436 27.48557480732327 32.639789810178044 20.691951176019252 80-81 19.159472903651704 27.84188745581695 32.56682571611106 20.431813924420293 82-83 19.227535074861272 27.83960776358497 32.486045309391685 20.446811852162078 84-85 19.01484198945642 27.57992548331554 32.278811822877685 21.126420704350352 86-87 19.096517302246603 27.59288311883771 32.24926751941421 21.061332059501474 88-89 19.3858612298317 27.12811598944807 32.199750516349354 21.286272264370872 90-91 18.91542376458232 26.987048297727195 32.859893566028816 21.237634371661667 92-93 19.20668690274332 27.110126299619896 32.67619250937984 21.00699428825694 94-95 18.725409842770226 27.045614792784217 32.6667713665461 21.56220399789946 96-97 18.746554152767395 27.070657659883224 32.634729559275115 21.54805862807427 98-99 18.928703766567224 27.589346259649133 31.9716094391989 21.51034053458474 100-101 19.00286275151317 27.211680026173727 32.54343203009979 21.242025192213315 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1438.0 1 961.5 2 669.0 3 836.0 4 626.0 5 530.5 6 1118.5 7 2825.5 8 4888.5 9 5637.0 10 4421.0 11 3019.5 12 2788.0 13 2876.0 14 2948.5 15 2966.5 16 3003.0 17 3062.5 18 3093.5 19 3246.5 20 3382.5 21 3643.5 22 4123.5 23 4601.0 24 5169.0 25 5899.5 26 6795.0 27 7669.0 28 8202.5 29 8829.0 30 9919.0 31 11117.5 32 12229.5 33 13616.0 34 15533.0 35 16783.0 36 17744.0 37 18847.5 38 19176.0 39 18718.0 40 18514.0 41 19250.0 42 19673.0 43 20027.0 44 20621.5 45 20841.0 46 20163.5 47 20239.5 48 23111.0 49 22532.5 50 18614.0 51 16302.5 52 14659.5 53 13562.5 54 12656.0 55 11453.5 56 9940.5 57 8602.0 58 7759.5 59 6821.0 60 5754.0 61 4915.5 62 3951.0 63 2926.5 64 2045.0 65 1453.0 66 1021.5 67 755.0 68 524.5 69 323.0 70 229.5 71 178.0 72 153.0 73 130.0 74 103.0 75 83.5 76 62.5 77 38.5 78 20.5 79 10.0 80 7.0 81 8.5 82 7.5 83 3.5 84 3.5 85 3.0 86 1.5 87 2.0 88 2.0 89 3.5 90 4.0 91 2.0 92 1.5 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26587882915325434 2 0.22717288166595623 3 0.23141910375317037 4 0.2292959927095633 5 0.23043920634842865 6 0.1850372932620621 7 0.17703479779000472 8 0.18046443870660075 9 0.18356744715494955 10-11 0.19148828450994515 12-13 0.19410134425592307 14-15 0.19059004522226522 16-17 0.18095438740897163 18-19 0.1745033961610886 20-21 0.1745850542781504 22-23 0.1802194643554153 24-25 0.18038278058953894 26-27 0.18258754975020783 28-29 0.18520060949618575 30-31 0.18364910527201136 32-33 0.18144433611134247 34-35 0.1857722163156184 36-37 0.1791579088336118 38-39 0.17385013122459414 40-41 0.17099209712743074 42-43 0.17670816532175748 44-45 0.17572826791701576 46-47 0.17940288318479722 48-49 0.18528226761324756 50-51 0.18070941305778618 52-53 0.1824242335160842 54-55 0.17948454130185904 56-57 0.16674587504021662 58-59 0.15817177274872654 60-61 0.1720536526492343 62-63 0.166582558806093 64-65 0.1712370714786162 66-67 0.16788908867908198 68-69 0.1722169688833579 70-71 0.1744217380440268 72-73 0.17760640460943738 74-75 0.18062775494072436 76-77 0.17425842180990317 78-79 0.17180867829804886 80-81 0.1753199773317067 82-83 0.1706654646591835 84-85 0.16927727666913273 86-87 0.16821572114732922 88-89 0.17091043901036895 90-91 0.16952225102031818 92-93 0.16731748185964931 94-95 0.16952225102031818 96-97 0.17409510557577954 98-99 0.1714003877127398 100-101 0.16478608023073318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 612309.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.96208502325673 #Duplication Level Percentage of deduplicated Percentage of total 1 79.31628184188595 42.800719444804436 2 11.542811957374187 12.457484005025805 3 3.7166982969607276 6.016823685191649 4 1.6594577279780618 3.5819119603861047 5 0.9117223218642503 2.459921872501986 6 0.5549071729728653 1.7966368828786075 7 0.382000160641819 1.4429467603216097 8 0.2751929886471268 1.1879989960944304 9 0.21899662826355382 1.0635763206747995 >10 1.2825535227710028 12.866103135365714 >50 0.0937297707959356 3.46840676762361 >100 0.039865579264123034 3.821142419733539 >500 0.003956126186515716 1.5602163449175597 >1k 9.129521968882421E-4 0.8348742129839883 >5k 6.086347979254948E-4 2.6913362463797377 >10k+ 3.043173989627474E-4 1.9499009451164198 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11874 1.939216963983871 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9742 1.5910267528323117 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6647 1.085563008219706 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2092 0.34165756178661427 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1903 0.31079079353725 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1089 0.17785137896062284 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 998 0.16298960165537335 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 980 0.16004990944114816 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 912 0.14894440552074198 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.13473589315198697 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 823 0.13440926068373973 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 815 0.13310273081075077 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 772 0.12608013274343507 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 644 0.10517565477561165 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.633162341236206E-4 0.0 18-19 0.0 0.0 0.0 1.633162341236206E-4 0.0 20-21 0.0 0.0 0.0 1.633162341236206E-4 0.0 22-23 0.0 0.0 0.0 4.082905853090515E-4 0.0 24-25 0.0 0.0 0.0 9.798974047417236E-4 0.0 26-27 0.0 0.0 0.0 0.0017148204582980162 0.0 28-29 0.0 0.0 0.0 0.004164563970152325 0.0 30-31 0.0 0.0 0.0 0.009472341579169995 0.0 32-33 0.0 0.0 0.0 0.015678358475867578 0.0 34-35 0.0 0.0 0.0 0.022619298426121454 0.0 36-37 0.0 0.0 0.0 0.02988687084462257 0.0 38-39 0.0 0.0 0.0 0.037807708199618165 0.0 40-41 0.0 0.0 0.0 0.04638181049110825 0.0 42-43 0.0 0.0 0.0 0.06622473293712815 0.0 44-45 0.0 0.0 0.0 0.08778247584144608 0.0 46-47 0.0 0.0 0.0 0.11219825284292735 0.0 48-49 0.0 0.0 0.0 0.14298336297522984 0.0 50-51 0.0 0.0 0.0 0.18364910527201134 0.0 52-53 0.0 0.0 0.0 0.2669403846750579 0.0 54-55 0.0 1.633162341236206E-4 0.0 0.3276940237690447 0.0 56-57 0.0 1.633162341236206E-4 0.0 0.44544502857217516 0.0 58-59 0.0 1.633162341236206E-4 0.0 0.6005954509896148 0.0 60-61 0.0 1.633162341236206E-4 0.0 0.744232078901339 0.0 62-63 0.0 1.633162341236206E-4 0.0 0.9491939527264828 0.0 64-65 0.0 1.633162341236206E-4 0.0 1.072742683841002 0.0 66-67 0.0 1.633162341236206E-4 0.0 1.3058766080524702 0.0 68-69 0.0 1.633162341236206E-4 0.0 1.5849840521697378 0.0 70-71 0.0 1.633162341236206E-4 0.0 1.7900275841119435 0.0 72-73 0.0 1.633162341236206E-4 0.0 2.1846812638716724 0.0 74-75 0.0 1.633162341236206E-4 0.0 2.5696176277010467 0.0 76-77 0.0 1.633162341236206E-4 0.0 2.8237376879974 0.0 78-79 0.0 1.633162341236206E-4 0.0 3.1023551834122967 0.0 80-81 0.0 1.633162341236206E-4 0.0 3.5781770315314656 0.0 82-83 0.0 1.633162341236206E-4 0.0 4.017334385089881 0.0 84-85 0.0 1.633162341236206E-4 0.0 4.5612591028386 0.0 86-87 0.0 1.633162341236206E-4 0.0 5.270949798222793 0.0 88-89 0.0 1.633162341236206E-4 0.0 6.240231647746481 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4935 0.0 44.706783 1 GTATCAA 7810 0.0 34.39854 1 GTATAGA 190 0.0 32.53362 1 TATCAAC 9780 0.0 27.411993 2 ATCAACG 9895 0.0 27.141449 3 TCAACGC 9940 0.0 26.975168 4 CAACGCA 10035 0.0 26.577673 5 GTACATG 4740 0.0 26.182116 1 AACGCAG 10210 0.0 26.109304 6 GAGTACT 6850 0.0 25.68663 12-13 CATGGGG 1150 0.0 25.630987 4 TACATGG 4810 0.0 25.199707 2 ATGGGCG 95 0.0012196944 25.02176 5 GTGTAGC 215 1.6389095E-9 24.327461 1 GTACTTT 7250 0.0 24.169203 14-15 ACATGGG 4820 0.0 24.161251 3 ACGCAGA 11145 0.0 23.66307 7 AGTACTT 6905 0.0 23.625038 12-13 CGCAGAG 11140 0.0 23.58838 8 GCAGAGT 11305 0.0 22.778011 9 >>END_MODULE