FastQCFastQC Report
Thu 26 May 2016
SRR937784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612309
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83281.3600975977815124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72461.1833894324597547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.8322595290939705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19260.3145470669220933No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17410.28433356360922346No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16510.2696351025380976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.2371351719474971No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9260.15123083279847266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.14094191004868456No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7030.11481131258890527No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.11089172296993838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60550.030.5330561
GTATCAA86350.028.4002531
ATGGGCG700.00837920927.1490655
GACCCGT700.0083892327.1424146
TAGGACA3150.025.6407834
GTATAGA2651.8189894E-1225.1082821
AGTACTT59500.024.14876612-13
GAGTACT59100.023.4281312-13
GTACATG57650.023.083071
TATCAAC107150.022.8353672
ATCAACG107950.022.7101483
TACATGG58750.022.5626052
TCAACGC109100.022.4707664
CAACGCA110500.022.1860685
GTACTTT62150.022.12554214-15
GTCTAGG1302.9283154E-421.9352571
AACGCAG112400.021.8479546
ACATGGG58600.021.8095933
TACTTTT66700.021.47078914-15
CCTTATA1805.1520274E-621.115942
Produced by FastQC (version 0.11.5)