##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937784_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 612309 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26914678699807 33.0 31.0 34.0 31.0 34.0 2 32.471247360401364 34.0 31.0 34.0 31.0 34.0 3 32.53545023836004 34.0 31.0 34.0 31.0 34.0 4 36.156731323563754 37.0 35.0 37.0 35.0 37.0 5 36.03372806867121 37.0 35.0 37.0 35.0 37.0 6 36.156097656575355 37.0 35.0 37.0 35.0 37.0 7 36.11723002601627 37.0 35.0 37.0 35.0 37.0 8 36.126939176134925 37.0 36.0 37.0 35.0 37.0 9 37.76742298414689 39.0 38.0 39.0 35.0 39.0 10-11 37.73889408778901 39.0 37.0 39.0 35.0 39.0 12-13 37.66560347798252 39.0 37.0 39.0 35.0 39.0 14-15 38.7980856071036 40.0 38.0 41.0 35.0 41.0 16-17 38.79456369251473 40.0 38.0 41.0 35.5 41.0 18-19 38.89387384474179 40.0 38.0 41.0 35.0 41.0 20-21 38.876454535210165 40.0 38.0 41.0 35.0 41.0 22-23 38.729995802772784 40.0 38.0 41.0 35.0 41.0 24-25 38.65568038359717 40.0 38.0 41.0 34.5 41.0 26-27 38.53570337852294 40.0 38.0 41.0 34.0 41.0 28-29 38.40269047164095 40.0 38.0 41.0 34.0 41.0 30-31 38.33174100005063 40.0 37.5 41.0 34.0 41.0 32-33 38.11767751249778 40.0 37.0 41.0 34.0 41.0 34-35 37.927028673431224 40.0 36.5 41.0 34.0 41.0 36-37 37.72018294684547 40.0 36.0 41.0 33.0 41.0 38-39 37.545057315832366 40.0 36.0 41.0 33.0 41.0 40-41 37.307980121147985 40.0 36.0 41.0 33.0 41.0 42-43 37.006142323565385 40.0 35.0 41.0 32.5 41.0 44-45 36.51431221817742 40.0 35.0 41.0 31.0 41.0 46-47 35.88466444229955 39.5 35.0 41.0 29.5 41.0 48-49 35.307355436552456 39.0 35.0 41.0 25.5 41.0 50-51 34.947528127138426 39.0 35.0 41.0 24.0 41.0 52-53 34.69390618135615 39.0 35.0 40.5 23.0 41.0 54-55 34.475530328641256 39.0 35.0 40.0 22.0 41.0 56-57 34.20273913987872 38.0 34.5 40.0 20.0 41.0 58-59 33.95583929029297 38.0 34.0 40.0 18.5 41.0 60-61 33.60922181447603 38.0 34.0 40.0 16.5 41.0 62-63 33.25960176969471 37.0 33.5 40.0 12.0 41.0 64-65 32.86021436888891 37.0 33.0 40.0 7.5 41.0 66-67 32.439213697659184 36.0 33.0 39.0 4.0 41.0 68-69 31.98753733817403 36.0 32.0 39.0 2.0 40.0 70-71 31.5614869289852 35.0 31.5 38.5 2.0 40.0 72-73 31.11583285563335 35.0 31.0 38.0 2.0 40.0 74-75 30.632527857666638 35.0 31.0 37.0 2.0 39.0 76-77 29.40520472506529 34.0 29.5 36.0 2.0 38.5 78-79 29.902520622757464 34.0 30.0 36.0 2.0 39.0 80-81 29.67348920234718 34.0 30.0 36.0 2.0 37.5 82-83 29.32274390871276 34.0 30.0 35.0 2.0 37.0 84-85 28.944965695425022 34.0 29.5 35.0 2.0 37.0 86-87 28.531067647217334 34.0 29.0 35.0 2.0 36.0 88-89 28.110169048633942 34.0 29.0 35.0 2.0 36.0 90-91 27.6359166695247 34.0 27.5 35.0 2.0 35.0 92-93 27.247590677256092 33.5 26.5 35.0 2.0 35.0 94-95 26.829954320449318 33.0 26.0 35.0 2.0 35.0 96-97 26.354936804783208 33.0 25.0 35.0 2.0 35.0 98-99 25.668871435827334 33.0 23.5 35.0 2.0 35.0 100-101 23.54476008028626 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1613300097471324 1101 2 0.09584397132830702 1101 3 0.10624516772345771 1101 4 0.05713531363754498 1101 5 -0.0204515089620827 1101 6 -0.09923224752222382 1101 7 0.025426068411078973 1101 8 -0.0221119792551292 1101 9 0.1946722663076983 1101 10-11 0.13222997571082118 1101 12-13 0.12112853546540947 1101 14-15 0.10865599195878417 1101 16-17 0.0718486523177404 1101 18-19 0.08826812212399204 1101 20-21 0.20858905844841757 1101 22-23 0.1596789894230497 1101 24-25 0.11814147960255639 1101 26-27 0.008610038313825896 1101 28-29 -0.042132360134104374 1101 30-31 0.16536326904140708 1101 32-33 0.09973324621224577 1101 34-35 -0.06297945407852268 1101 36-37 0.06816234423548906 1101 38-39 0.12467526824722341 1101 40-41 0.02372368333485042 1101 42-43 0.12136336256317293 1101 44-45 0.4605400633374259 1101 46-47 0.43663577422610445 1101 48-49 0.34725857797842963 1101 50-51 0.6020053991607952 1101 52-53 0.4510109935313267 1101 54-55 0.5481122111497783 1101 56-57 0.5726123660558144 1101 58-59 0.7086129272808463 1101 60-61 0.8076943234084979 1101 62-63 0.6729710260563166 1101 64-65 0.598851396240299 1101 66-67 0.8328849314023543 1101 68-69 0.7912749965795598 1101 70-71 0.7851412717610025 1101 72-73 0.7959187912080381 1101 74-75 0.9606052965734477 1101 76-77 0.808003960012055 1101 78-79 0.8629630941297819 1101 80-81 0.8371350217190034 1101 82-83 0.6319062453940987 1101 84-85 0.9755848978670905 1101 86-87 1.0632316878448087 1101 88-89 1.1276493017326423 1101 90-91 1.084957153713816 1101 92-93 1.1784374022296795 1101 94-95 1.1424097637266648 1101 96-97 1.323309417802374 1101 98-99 1.4621312125520127 1101 100-101 1.921032121349647 1102 1 0.07948024856606395 1102 2 0.20042495265700921 1102 3 0.03427382599527817 1102 4 0.04712662931274991 1102 5 -0.0077941055752006605 1102 6 0.01555281543912912 1102 7 0.06133575143322645 1102 8 0.022243209628939553 1102 9 0.13177604398897813 1102 10-11 0.03402111769953109 1102 12-13 0.18910508778846946 1102 14-15 0.19724696026099764 1102 16-17 0.17652733230036688 1102 18-19 0.11154211257123592 1102 20-21 0.15008927555653173 1102 22-23 0.15578189867185444 1102 24-25 0.16823917825648493 1102 26-27 0.11590487114057169 1102 28-29 0.00919199939693982 1102 30-31 0.11518958254552558 1102 32-33 0.24288348503117874 1102 34-35 0.38126718074561694 1102 36-37 0.27665127172137716 1102 38-39 0.17403479929368615 1102 40-41 0.2945552074338522 1102 42-43 0.037570483709501445 1102 44-45 0.1399365027642574 1102 46-47 0.24250854886788176 1102 48-49 0.39848523885554954 1102 50-51 0.5850275441890176 1102 52-53 0.4683687877128193 1102 54-55 0.4081860279105243 1102 56-57 0.6352806248486971 1102 58-59 0.6697939954527996 1102 60-61 0.6500521175900076 1102 62-63 0.5565793630081188 1102 64-65 0.6460832677122852 1102 66-67 0.6431867116889407 1102 68-69 0.7621282314905429 1102 70-71 0.9457470034153665 1102 72-73 0.8468306453113854 1102 74-75 0.7531801988747908 1102 76-77 0.5725067824176122 1102 78-79 0.8637338279552296 1102 80-81 0.8929643747368061 1102 82-83 0.8623448037961801 1102 84-85 0.9652071297385625 1102 86-87 0.9464166639629177 1102 88-89 0.8982854285237885 1102 90-91 1.1348920212778637 1102 92-93 1.433029238964373 1102 94-95 1.641801860991098 1102 96-97 1.6442929175852683 1102 98-99 1.6308459324825364 1102 100-101 1.9778601717187314 1103 1 0.12934696130734125 1103 2 0.2686433462987097 1103 3 0.05737448255844413 1103 4 0.03903313076904169 1103 5 -0.052065221732576106 1103 6 0.05349278680705538 1103 7 -0.02204777031234073 1103 8 0.02655036271182354 1103 9 0.2571302425376274 1103 10-11 0.07467990231719313 1103 12-13 0.120779323855011 1103 14-15 0.23288772641591038 1103 16-17 0.057984359500842686 1103 18-19 0.11348711923560018 1103 20-21 0.1250102905143322 1103 22-23 0.18615041325940496 1103 24-25 0.24087456355539416 1103 26-27 0.24071974994789969 1103 28-29 0.14072430921576995 1103 30-31 0.3012894492582632 1103 32-33 0.37511678709959995 1103 34-35 0.3183383166492604 1103 36-37 0.5438465622382367 1103 38-39 0.32548614944425225 1103 40-41 0.29674087164869434 1103 42-43 0.30253197850143465 1103 44-45 0.5361970546722361 1103 46-47 0.30921141701222155 1103 48-49 0.48000397312024035 1103 50-51 0.517632322784074 1103 52-53 0.6278766121685635 1103 54-55 0.49032183281746455 1103 56-57 0.4182667531006601 1103 58-59 0.508502836840961 1103 60-61 0.5807896404216137 1103 62-63 0.6216321842550983 1103 64-65 0.5491402356739812 1103 66-67 0.6117617721618629 1103 68-69 0.4155801246632791 1103 70-71 0.7157499653544441 1103 72-73 0.694037783584573 1103 74-75 0.6262783871553879 1103 76-77 0.6084289327853867 1103 78-79 0.6478380882109427 1103 80-81 0.5967519543389166 1103 82-83 0.6914261090240039 1103 84-85 0.7480007319501425 1103 86-87 0.5965808047537529 1103 88-89 0.655606354623405 1103 90-91 0.809109575703495 1103 92-93 0.891787939660432 1103 94-95 0.6738648515622607 1103 96-97 0.8442632981944378 1103 98-99 0.9433329739990484 1103 100-101 1.4959847706228118 1104 1 0.15825271583744183 1104 2 0.2566104826572513 1104 3 0.16824098967428824 1104 4 0.08443725803735447 1104 5 -0.12450903748530351 1104 6 0.04367310359459964 1104 7 0.12389947352115627 1104 8 0.1028920380998457 1104 9 0.181872973362168 1104 10-11 0.10128830770504038 1104 12-13 0.24488315542362926 1104 14-15 0.13240659456202764 1104 16-17 0.01317920028495223 1104 18-19 0.17507512839106454 1104 20-21 0.06903418137947881 1104 22-23 0.2608922306250392 1104 24-25 0.09825557248380079 1104 26-27 9.328729594955121E-4 1104 28-29 -0.00910970225205432 1104 30-31 0.07740328189693457 1104 32-33 0.07643660472100322 1104 34-35 0.04429999313343558 1104 36-37 0.14932496676481577 1104 38-39 -0.018949189409859457 1104 40-41 -0.023648966512489267 1104 42-43 0.13505450831281252 1104 44-45 0.2985763802263861 1104 46-47 0.3969804632828371 1104 48-49 0.2960614061963298 1104 50-51 0.12930332620562268 1104 52-53 0.32174456254948325 1104 54-55 0.3596058637289232 1104 56-57 0.3732912990463362 1104 58-59 0.3044067566236137 1104 60-61 0.45067198691484833 1104 62-63 0.5442830941906536 1104 64-65 0.5423754200230348 1104 66-67 0.40064967509510296 1104 68-69 0.6012380369137986 1104 70-71 0.41018948002068356 1104 72-73 0.5010650229290761 1104 74-75 0.502980356833767 1104 76-77 0.20495478758718022 1104 78-79 0.591940275553533 1104 80-81 0.4862110300751006 1104 82-83 0.3649128338562555 1104 84-85 0.5613634612609495 1104 86-87 0.5902042091367896 1104 88-89 0.6516288935693773 1104 90-91 0.5443617817067441 1104 92-93 0.5958807575245544 1104 94-95 0.8708124633892034 1104 96-97 0.9791707387086923 1104 98-99 1.123066452789839 1104 100-101 1.8776004967312971 1105 1 0.1210062940539558 1105 2 0.21951274918427544 1105 3 0.09584756040467113 1105 4 0.12308652170698053 1105 5 0.03439836202078794 1105 6 0.07803141656410162 1105 7 0.010571505896422195 1105 8 0.01542613944062765 1105 9 3.196976940458285E-4 1105 10-11 0.056230092999122405 1105 12-13 0.09422553288506919 1105 14-15 0.2134061678912289 1105 16-17 0.16606633939256454 1105 18-19 0.2598638293724065 1105 20-21 0.19597763456851425 1105 22-23 0.2993928059841551 1105 24-25 0.25190877962601377 1105 26-27 0.17741135194272317 1105 28-29 0.08565323686330828 1105 30-31 0.13309432698181922 1105 32-33 0.20179458799034222 1105 34-35 0.23343152410585333 1105 36-37 0.4498507092366566 1105 38-39 0.29274353003469855 1105 40-41 0.2570276945945267 1105 42-43 0.2222488891876182 1105 44-45 0.21598810462290174 1105 46-47 0.260550857500796 1105 48-49 0.04644928874549237 1105 50-51 0.1185137266297076 1105 52-53 0.08275800267981026 1105 54-55 -0.12181213790018575 1105 56-57 0.19100023844615066 1105 58-59 0.1585015034009487 1105 60-61 0.027228688878828677 1105 62-63 0.11363487780391068 1105 64-65 0.31275299136110135 1105 66-67 0.15271838202398413 1105 68-69 0.04202857387254255 1105 70-71 0.20573416409034806 1105 72-73 0.3516453921932623 1105 74-75 0.3588019890435348 1105 76-77 0.3026761392285735 1105 78-79 0.5185577457835464 1105 80-81 0.47026016832937145 1105 82-83 0.3522323068531641 1105 84-85 0.5453245178530146 1105 86-87 0.9555111858509093 1105 88-89 0.7504589428304627 1105 90-91 0.660050984349521 1105 92-93 0.9417936067804646 1105 94-95 1.1730747883572015 1105 96-97 1.3323523453182098 1105 98-99 1.4019329953340893 1105 100-101 1.8523832825037658 1106 1 -0.2514700325495802 1106 2 0.017335987824026233 1106 3 -0.08623659181973409 1106 4 0.1393601206588002 1106 5 -0.07321136938293904 1106 6 0.010443125217548754 1106 7 -0.027000705407253633 1106 8 -0.0713242030047283 1106 9 -0.010556664642926705 1106 10-11 -0.07819087656790913 1106 12-13 -0.056372257597935516 1106 14-15 -0.056068550708019416 1106 16-17 -0.030393073388090386 1106 18-19 -0.04131314043284817 1106 20-21 0.05425364552677081 1106 22-23 0.1362994821797372 1106 24-25 -0.11517775482697346 1106 26-27 -0.22227449166396696 1106 28-29 -0.10214081947638931 1106 30-31 -0.3177312641086445 1106 32-33 -0.06475073390830488 1106 34-35 -0.28412252270494065 1106 36-37 -0.24013574183831565 1106 38-39 -0.28546801186274706 1106 40-41 -0.21588189983106076 1106 42-43 -0.06928377712171141 1106 44-45 -0.11732289531754247 1106 46-47 0.3928613214398098 1106 48-49 0.40217557068223897 1106 50-51 0.304223332966437 1106 52-53 0.6227012758618713 1106 54-55 0.5438904706401004 1106 56-57 0.6045067300190183 1106 58-59 0.25673865425557807 1106 60-61 0.24194937257843208 1106 62-63 0.24496267245580583 1106 64-65 0.3147443855603669 1106 66-67 0.2753138200562617 1106 68-69 -0.03498068704104185 1106 70-71 0.24076409083681938 1106 72-73 0.4259150902457911 1106 74-75 0.5786184085539858 1106 76-77 0.39529644177159895 1106 78-79 0.6467290875737959 1106 80-81 0.44917372320996307 1106 82-83 0.2694498638777638 1106 84-85 0.6114313608045983 1106 86-87 0.815573868240282 1106 88-89 0.9014438454568712 1106 90-91 0.8447779213991282 1106 92-93 1.0505162855964976 1106 94-95 1.0631721619501988 1106 96-97 1.3169199708318793 1106 98-99 1.2871021335629003 1106 100-101 1.8221662019248832 1107 1 0.12465269823572811 1107 2 0.1315283184167768 1107 3 0.2560680308540384 1107 4 0.13628805052692172 1107 5 -0.10047995065661297 1107 6 0.03479799048984944 1107 7 -0.024441702658222653 1107 8 0.052350372922909116 1107 9 0.21215891867035452 1107 10-11 0.04346943481964871 1107 12-13 0.081503516753763 1107 14-15 0.1769210485926891 1107 16-17 0.3251942937527943 1107 18-19 0.1523317916015614 1107 20-21 0.07303444540515613 1107 22-23 0.07537431400765371 1107 24-25 0.26343113580733757 1107 26-27 -0.09906547688002831 1107 28-29 -0.13920835434596768 1107 30-31 -0.09663089292656224 1107 32-33 0.09490190047101521 1107 34-35 0.21323929748077575 1107 36-37 0.07649650919731243 1107 38-39 0.22025807349646698 1107 40-41 0.13586379610367771 1107 42-43 0.38661459753149074 1107 44-45 0.20529830893395484 1107 46-47 0.1933382840809017 1107 48-49 0.3101496512781594 1107 50-51 0.2627820570121955 1107 52-53 0.27100625324889904 1107 54-55 0.17293008450939595 1107 56-57 0.5816625631517738 1107 58-59 0.37434193281949746 1107 60-61 0.5947433728571312 1107 62-63 0.4790797531657418 1107 64-65 0.45843175650175993 1107 66-67 0.33031020958208757 1107 68-69 0.5474648074672359 1107 70-71 0.4655826170032533 1107 72-73 0.40389119777457694 1107 74-75 0.44206330765073787 1107 76-77 0.45954704668437785 1107 78-79 0.736446203755051 1107 80-81 0.8207136854583368 1107 82-83 0.724790831991573 1107 84-85 0.6472130601344439 1107 86-87 0.6874495412453392 1107 88-89 0.8992712267862863 1107 90-91 0.8628936339293745 1107 92-93 0.8359020996000694 1107 94-95 1.1392138154206854 1107 96-97 1.2811446056672544 1107 98-99 1.4931176902726833 1107 100-101 2.0345032555245908 1108 1 0.11546606416889915 1108 2 0.06385757677048076 1108 3 -0.0587616349976301 1108 4 0.11533939527019754 1108 5 0.023099511446076804 1108 6 0.1423187728859432 1108 7 -0.012451482609328934 1108 8 -0.05479225136397048 1108 9 -0.078266509202507 1108 10-11 0.0333048056427927 1108 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94-95 -0.42195465972867297 2316 96-97 -0.40447913563552973 2316 98-99 -0.4863423917657492 2316 100-101 -1.109916043040073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 7.0 5 11.0 6 32.0 7 105.0 8 187.0 9 366.0 10 668.0 11 1130.0 12 1790.0 13 2736.0 14 3461.0 15 5291.0 16 7726.0 17 12192.0 18 11183.0 19 5554.0 20 4093.0 21 3924.0 22 3916.0 23 4182.0 24 4492.0 25 5059.0 26 5737.0 27 6728.0 28 8377.0 29 9956.0 30 11993.0 31 15112.0 32 19054.0 33 25535.0 34 35933.0 35 54094.0 36 83062.0 37 131353.0 38 115857.0 39 11408.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.023805866994316 20.43595617236951 15.581814082224192 18.958423878411985 2 15.495280977415 22.608192922201045 40.129248467685436 21.767277632698523 3 19.35983302548223 28.17972624932836 31.450623786356235 21.00981693883317 4 11.902323826695344 18.669821936309937 37.93346169989336 31.49439253710137 5 11.755117240838619 40.39092257335311 33.20555203480965 14.648408150998621 6 28.106723892675106 38.76539459651907 17.509133460393365 15.618748050412457 7 25.814743862984212 37.122596597469574 20.25333614237256 16.80932339717365 8 27.335871267611616 34.36794167650647 19.106202913888247 19.189984141993666 9 27.90584492470305 16.71051707552886 20.72874969990642 34.65488829986167 10-11 24.44337744504817 28.856590381653707 25.827972477948226 20.872059695349897 12-13 26.553485466504977 27.14682933401928 27.354818162887835 18.944867036587905 14-15 23.04334902802343 27.17516809323397 28.255668298195847 21.52581458054675 16-17 19.370693555051453 30.92695028163884 28.566867382318406 21.135488780991295 18-19 19.355750119629143 27.692063974235232 33.945850869413974 19.006335036721655 20-21 21.111807927043373 26.239692704173873 34.12051758180918 18.527981786973573 22-23 20.372508606779594 26.930890989502014 33.99408467633936 18.702515727379033 24-25 19.784748676114532 27.124606508984456 34.536078685954365 18.554566128946647 26-27 19.750514657427196 27.124916605490277 34.574526723398066 18.550042013684457 28-29 18.981571836520377 27.54735655029875 34.81197426410244 18.65909734907843 30-31 19.328165459078225 27.30731322527778 34.58550714223304 18.779014173410953 32-33 19.124984280812466 27.767189441932093 34.153180828633914 18.95464544862153 34-35 19.487383004332777 27.31268036236606 34.48463112578779 18.715305507513364 36-37 19.79368260143163 27.181129135779486 34.19711289561317 18.82807536717572 38-39 19.656823597236038 27.616040267250686 34.07928023269297 18.647855902820307 40-41 19.181573355936298 28.00595777622083 34.20870834823594 18.60376051960693 42-43 19.259207839934668 28.228746427113105 33.324948958758675 19.18709677419355 44-45 19.28650403636073 28.449443009983522 32.85228536572221 19.411767587933543 46-47 19.769593456898395 28.876759936567975 31.993895239168456 19.35975136736517 48-49 20.03138938019856 29.3657287415341 31.237251126473726 19.36563075179362 50-51 20.05392702050762 29.622951810278796 30.794582473881647 19.52853869533193 52-53 20.121784915785984 30.29924433578471 30.16303859652561 19.415932151903696 54-55 19.584147873051023 30.27033736234483 30.236857534349486 19.908657230254658 56-57 19.862520394114735 30.481178620598428 29.9137363651359 19.742564620150937 58-59 20.016445944776248 29.908428587526885 30.258006986668494 19.81711848102837 60-61 19.869706308416177 29.809132317179728 30.78894806380439 19.532213310599715 62-63 19.810259199195176 29.734823430653478 31.058256533874236 19.396660836277107 64-65 19.643839956623207 29.902712519332557 30.89828828255015 19.55515924149408 66-67 19.393067879126388 30.077052599259524 30.624570274158962 19.905309247455126 68-69 19.60995183804256 30.17651218584081 30.385801939870227 19.827734036246405 70-71 19.669888895965926 30.539564174297617 30.077542547961894 19.713004381774564 72-73 19.719389817387803 30.231901076009887 29.931562276205543 20.117146830396766 74-75 19.55025975447037 30.76575715855883 29.537537419832145 20.14644566713865 76-77 19.60464406043354 30.22305731256604 29.976041508454067 20.196257118546352 78-79 19.54919819894857 30.391436349947494 29.49809654929129 20.561268901812646 80-81 19.595988300024988 30.75946948354507 29.481438293410676 20.16310392301926 82-83 19.489195397418133 30.76333253580507 29.490118134894423 20.257353931882378 84-85 19.18160468261071 30.74825088027594 29.27987222116974 20.79027221594361 86-87 19.157974160105436 30.394212725927595 29.37087320290899 21.07693991105798 88-89 19.178062056902643 30.280707943211677 29.32710445216386 21.21412554772182 90-91 19.117158273058163 30.03773714336113 29.488543702323273 21.356560881257437 92-93 19.158578958757793 30.305366263948493 29.508858030614206 21.027196746679515 94-95 18.744539113421492 30.340808317369174 29.483071455751915 21.43158111345742 96-97 18.7964736758728 30.31002320723687 29.29509447027563 21.5984086466147 98-99 18.71634653858277 30.967145565868105 28.641433137298772 21.675074758250354 100-101 18.251406731075487 31.353891887918856 28.95693647047294 21.43776491053272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1348.0 1 893.0 2 496.5 3 597.5 4 594.5 5 635.5 6 1087.5 7 2161.0 8 3166.0 9 3544.5 10 3258.5 11 2862.0 12 2890.0 13 3053.5 14 3159.5 15 3125.0 16 3033.0 17 3048.0 18 3122.0 19 3136.5 20 3240.0 21 3519.5 22 3997.0 23 4505.5 24 4875.5 25 5507.5 26 6451.5 27 7450.0 28 8141.5 29 8766.5 30 9879.5 31 10821.0 32 11809.0 33 13304.5 34 15532.5 35 16793.0 36 17917.5 37 19179.0 38 19417.0 39 19284.0 40 18963.0 41 19481.0 42 19726.5 43 20223.5 44 21079.5 45 21153.5 46 20289.5 47 20112.5 48 23706.0 49 23093.5 50 18661.0 51 16560.5 52 14799.5 53 13787.0 54 12858.0 55 11688.5 56 10169.0 57 8885.0 58 8266.0 59 7408.5 60 6416.0 61 5455.5 62 4317.0 63 3137.5 64 1971.5 65 1422.0 66 1038.0 67 765.5 68 530.0 69 308.0 70 228.5 71 166.0 72 161.5 73 158.5 74 119.5 75 95.0 76 73.5 77 43.0 78 22.5 79 13.5 80 9.0 81 5.0 82 4.5 83 4.0 84 2.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04491196438399566 2 0.0 3 0.0 4 0.0 5 0.010615555218035339 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0026947178630397396 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.633162341236206E-4 24-25 2.449743511854309E-4 26-27 0.001388187990050775 28-29 0.0020414529265452576 30-31 8.16581170618103E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009635657813293615 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.16581170618103E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.16581170618103E-5 84-85 1.633162341236206E-4 86-87 0.0 88-89 0.0 90-91 0.007757521120871979 92-93 0.0018781366924216368 94-95 0.0 96-97 0.0 98-99 3.266324682472412E-4 100-101 0.012085401325147923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 612309.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.11723788807826 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6460313486421 40.46696686862362 2 12.188738562891324 12.704867744756005 3 4.02827340080637 6.298274493241308 4 1.8931467073034576 3.9466230920626537 5 1.0419098324885796 2.7150731298867536 6 0.6296409347363343 1.9689087827835294 7 0.4625125493332457 1.6873413591875608 8 0.3117998222747718 1.3000116408763844 9 0.24303522760664203 1.1399692295122748 >10 1.3886973678586123 13.493751595409059 >50 0.10628880965125806 3.8115561050492963 >100 0.055194480901561746 5.0680476877253975 >500 0.002523176269785621 0.8881247293973776 >1k 0.0012615881348928106 1.1128270253600308 >5k 9.46191101169608E-4 3.3976565161287797 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8328 1.3600975977815124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7246 1.1833894324597547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.8322595290939705 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1926 0.3145470669220933 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1741 0.28433356360922346 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1651 0.2696351025380976 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1452 0.2371351719474971 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 926 0.15123083279847266 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 863 0.14094191004868456 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 703 0.11481131258890527 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.11089172296993838 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.633162341236206E-4 0.0 18-19 0.0 0.0 0.0 1.633162341236206E-4 0.0 20-21 0.0 0.0 0.0 2.449743511854309E-4 0.0 22-23 0.0 0.0 0.0 5.71606819432672E-4 0.0 24-25 0.0 0.0 0.0 0.0011432136388653442 0.0 26-27 0.0 0.0 0.0 0.0017148204582980162 0.0 28-29 0.0 0.0 0.0 0.004327880204275946 0.0 30-31 0.0 0.0 0.0 0.009635657813293615 0.0 32-33 0.0 0.0 0.0 0.015760016592929388 0.0 34-35 0.0 0.0 0.0 0.022864272777306884 0.0 36-37 0.0 0.0 0.0 0.030131845195808 0.0 38-39 0.0 0.0 0.0 0.037971024433741785 0.0 40-41 0.0 0.0 0.0 0.04687175919347911 0.0 42-43 0.0 0.0 0.0 0.06671468163949901 0.0 44-45 0.0 0.0 0.0 0.0880274501926315 0.0 46-47 0.0 0.0 0.0 0.1126065434282364 0.0 48-49 0.0 0.0 0.0 0.14330999544347706 0.0 50-51 0.0 0.0 0.0 0.18430237020850582 0.0 52-53 0.0 0.0 0.0 0.268165256430985 0.0 54-55 0.0 0.0 0.0 0.32908221175909547 0.0 56-57 0.0 0.0 0.0 0.4477314558499058 0.0 58-59 0.0 0.0 0.0 0.6050049893109525 0.0 60-61 0.0 0.0 0.0 0.7492132240421094 0.0 62-63 0.0 0.0 0.0 0.9561348926767368 0.0 64-65 0.0 0.0 0.0 1.0814801023666156 0.0 66-67 0.0 0.0 0.0 1.3182069837288037 0.0 68-69 0.0 0.0 0.0 1.598702615836122 0.0 70-71 0.0 0.0 0.0 1.8041544383636365 0.0 72-73 0.0 0.0 0.0 2.2031359983276415 0.0 74-75 0.0 0.0 0.0 2.590848738137117 0.0 76-77 0.0 0.0 0.0 2.8475818581794483 0.0 78-79 0.0 0.0 0.0 3.1296289945109415 0.0 80-81 0.0 0.0 0.0 3.611411885175622 0.0 82-83 0.0 0.0 0.0 4.056285306928364 0.0 84-85 0.0 0.0 0.0 4.60372132371074 0.0 86-87 0.0 0.0 0.0 5.321169540215806 0.0 88-89 0.0 0.0 0.0 6.3075995943224745 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6055 0.0 30.533056 1 GTATCAA 8635 0.0 28.400253 1 ATGGGCG 70 0.008379209 27.149065 5 GACCCGT 70 0.00838923 27.142414 6 TAGGACA 315 0.0 25.640783 4 GTATAGA 265 1.8189894E-12 25.108282 1 AGTACTT 5950 0.0 24.148766 12-13 GAGTACT 5910 0.0 23.42813 12-13 GTACATG 5765 0.0 23.08307 1 TATCAAC 10715 0.0 22.835367 2 ATCAACG 10795 0.0 22.710148 3 TACATGG 5875 0.0 22.562605 2 TCAACGC 10910 0.0 22.470766 4 CAACGCA 11050 0.0 22.186068 5 GTACTTT 6215 0.0 22.125542 14-15 GTCTAGG 130 2.9283154E-4 21.935257 1 AACGCAG 11240 0.0 21.847954 6 ACATGGG 5860 0.0 21.809593 3 TACTTTT 6670 0.0 21.470789 14-15 CCTTATA 180 5.1520274E-6 21.11594 2 >>END_MODULE