FastQCFastQC Report
Thu 26 May 2016
SRR937783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891767
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192080.6642305552279972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147920.5115211564417189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102200.3534171321548382No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60360.2087305097540708No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46860.1620462506142438No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37610.1300588878702883No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35730.1235576725234087No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33010.11415165883005096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165700.027.9529781
GTATCAA231900.027.6632251
GTACATG140000.026.8682921
TACATGG143150.025.9708712
ACATGGG143400.024.765243
GAGTACT149800.023.1168812-13
ATCAACG278950.022.8205093
TCAACGC279600.022.7864344
TATCAAC280900.022.7297882
CAACGCA281350.022.6278065
AACGCAG286600.022.310476
CATGGGA98500.021.9626084
AGTACTT155350.021.75590712-13
GTACTTT162450.021.19888514-15
CATGGGG45050.020.4746114
ACGCAGA314200.020.0932967
AGAGTAC243000.020.0662510-11
CGCAGAG315800.019.9012378
ATGGGAG39600.019.8105955
GCAGAGT321950.019.1079319
Produced by FastQC (version 0.11.5)