##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937783_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2891767 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.819672885125254 33.0 31.0 34.0 30.0 34.0 2 31.950375669962344 33.0 31.0 34.0 30.0 34.0 3 31.965283510047662 34.0 31.0 34.0 30.0 34.0 4 35.5688528847587 37.0 35.0 37.0 33.0 37.0 5 35.56994391318526 37.0 35.0 37.0 33.0 37.0 6 35.644770826971886 37.0 35.0 37.0 33.0 37.0 7 35.59003716412837 37.0 35.0 37.0 33.0 37.0 8 35.58355496829447 37.0 35.0 37.0 33.0 37.0 9 37.22170147179908 39.0 37.0 39.0 34.0 39.0 10-11 37.10690487857424 39.0 37.0 39.0 33.0 39.0 12-13 37.00295407617557 39.0 37.0 39.0 33.0 39.0 14-15 38.146373653202346 40.0 38.0 41.0 33.0 41.0 16-17 38.07694568753291 40.0 38.0 41.0 33.0 41.0 18-19 38.05398256498535 40.0 38.0 41.0 33.0 41.0 20-21 37.982517782380114 40.0 38.0 41.0 33.0 41.0 22-23 37.901462496805586 40.0 38.0 41.0 33.0 41.0 24-25 37.930679926840575 40.0 38.0 41.0 33.0 41.0 26-27 37.83936223077447 40.0 38.0 41.0 33.0 41.0 28-29 37.72614633198318 40.0 37.0 41.0 33.0 41.0 30-31 37.47690425957555 40.0 37.0 41.0 32.5 41.0 32-33 37.309089563578254 39.5 36.5 41.0 32.0 41.0 34-35 37.28403187393729 40.0 36.0 41.0 32.0 41.0 36-37 37.14242883330503 40.0 36.0 41.0 31.5 41.0 38-39 36.99120537719671 40.0 36.0 41.0 31.0 41.0 40-41 36.766272317237174 39.5 36.0 41.0 30.5 41.0 42-43 36.48634934972286 39.0 35.5 41.0 30.0 41.0 44-45 36.19792016438392 39.0 35.0 41.0 30.0 41.0 46-47 35.78174659300006 39.0 35.0 41.0 28.5 41.0 48-49 35.41434026323698 39.0 35.0 40.5 26.5 41.0 50-51 34.50908078002135 38.0 34.0 39.5 25.0 40.5 52-53 34.596298906516324 38.0 34.5 40.0 25.0 40.5 54-55 34.90363210452294 39.0 35.0 40.0 25.5 41.0 56-57 34.69575660832979 38.0 34.5 40.0 24.0 41.0 58-59 34.47036206582342 38.0 34.0 40.0 23.0 41.0 60-61 34.16483105312426 38.0 34.0 40.0 22.5 41.0 62-63 33.824390588868326 38.0 34.0 40.0 22.0 41.0 64-65 33.45202345140532 37.0 33.0 40.0 20.5 41.0 66-67 33.02641620158194 36.5 32.5 39.0 18.5 41.0 68-69 32.54548430077527 36.0 32.0 39.0 17.5 40.5 70-71 32.10107159394239 35.5 31.5 39.0 13.0 40.0 72-73 31.65317987237561 35.0 31.0 38.0 9.0 40.0 74-75 31.13660488552501 35.0 31.0 37.0 7.0 39.5 76-77 30.667779077636617 35.0 30.0 37.0 4.5 39.0 78-79 30.127123485398375 34.0 29.5 36.0 2.0 39.0 80-81 29.566869322459244 34.0 29.0 36.0 2.0 37.5 82-83 29.070941054379553 34.0 29.0 35.0 2.0 37.0 84-85 28.57460144610544 34.0 28.5 35.0 2.0 36.5 86-87 28.071277526854686 34.0 27.0 35.0 2.0 36.0 88-89 27.631835656192216 33.0 26.5 35.0 2.0 36.0 90-91 27.10718048860783 33.0 25.5 35.0 2.0 35.0 92-93 26.5267440288239 32.5 25.0 35.0 2.0 35.0 94-95 25.83688346951881 32.0 23.5 34.5 2.0 35.0 96-97 25.066806212257074 32.0 19.0 34.0 2.0 35.0 98-99 24.085364415597798 31.5 4.5 34.0 2.0 35.0 100-101 21.318892566378963 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14831514117079792 1101 2 0.2121899154989073 1101 3 0.22236966488368992 1101 4 0.059426407168921 1101 5 -0.020173548829859556 1101 6 0.07766077328837184 1101 7 0.19293633568992163 1101 8 0.21968497176303714 1101 9 0.22424235950273896 1101 10-11 0.3006647678579313 1101 12-13 0.19781117074201404 1101 14-15 0.4467675932777979 1101 16-17 0.40598561695514945 1101 18-19 0.40189721380883014 1101 20-21 0.33979564546343966 1101 22-23 0.34968726894027924 1101 24-25 0.37259312179291726 1101 26-27 0.30876217918714843 1101 28-29 0.32408388903222374 1101 30-31 0.45409614280276855 1101 32-33 0.3617518757279612 1101 34-35 0.359561359587083 1101 36-37 0.35523060156320696 1101 38-39 0.40231441317437344 1101 40-41 0.426359529199992 1101 42-43 0.3352252546004735 1101 44-45 0.40582445024223546 1101 46-47 0.5888795725276239 1101 48-49 0.6886393587534911 1101 50-51 0.5334828732995831 1101 52-53 0.4149710134505966 1101 54-55 0.39170519253416103 1101 56-57 0.2638246987357178 1101 58-59 0.3813987239550798 1101 60-61 0.43334117879126666 1101 62-63 0.35464006359936207 1101 64-65 0.3871404174171076 1101 66-67 0.3977466193893662 1101 68-69 0.2613227613852871 1101 70-71 0.530724859935539 1101 72-73 0.5676686634736434 1101 74-75 0.5727384638933941 1101 76-77 0.4188723537797685 1101 78-79 0.6231077165330952 1101 80-81 0.5827698710101821 1101 82-83 0.609411113292964 1101 84-85 0.5227383005620077 1101 86-87 0.7087907272454679 1101 88-89 0.7450834944230529 1101 90-91 0.8912906955698112 1101 92-93 0.7756255643669867 1101 94-95 0.6812099960911375 1101 96-97 1.0436393544822131 1101 98-99 1.1947182687099094 1101 100-101 1.2781966843632482 1102 1 0.2851487349888586 1102 2 0.4281828321994503 1102 3 0.3919549941288274 1102 4 0.30740603051985715 1102 5 0.2184179166550635 1102 6 0.23364316525547224 1102 7 0.18861894948314273 1102 8 0.1304282878191998 1102 9 0.2421986525427542 1102 10-11 0.32403078843793054 1102 12-13 0.3866428629557248 1102 14-15 0.4608508176802104 1102 16-17 0.5752702469562934 1102 18-19 0.528735793464655 1102 20-21 0.6397470657722621 1102 22-23 0.5927236254280999 1102 24-25 0.6373667329470578 1102 26-27 0.5199555513080014 1102 28-29 0.5514522295445659 1102 30-31 0.5300993451327685 1102 32-33 0.6090630475183474 1102 34-35 0.678621398180546 1102 36-37 0.752274818958206 1102 38-39 0.7905874649291533 1102 40-41 0.7883332743355282 1102 42-43 0.7343559660928278 1102 44-45 0.7261519998235642 1102 46-47 0.8026754875088997 1102 48-49 0.7093165511238624 1102 50-51 0.6780812129199703 1102 52-53 0.7304471952636007 1102 54-55 0.7289015798601568 1102 56-57 0.7200638363978555 1102 58-59 0.7868615845576628 1102 60-61 0.78311578690505 1102 62-63 0.8274971357648937 1102 64-65 0.7469625905895256 1102 66-67 0.7681762767658356 1102 68-69 0.7853284370900155 1102 70-71 0.7067673480889738 1102 72-73 0.7590425507824854 1102 74-75 0.7469177082957081 1102 76-77 0.7329256589972459 1102 78-79 0.8150677724460031 1102 80-81 0.8532922695432923 1102 82-83 0.8479179567607389 1102 84-85 0.910160973815195 1102 86-87 0.850437432195104 1102 88-89 0.8529732396276124 1102 90-91 0.9944642707128146 1102 92-93 1.088230211342438 1102 94-95 1.1597064915545054 1102 96-97 1.3776090336025923 1102 98-99 1.5013892385551593 1102 100-101 1.6306540391288884 1103 1 0.3202201678317351 1103 2 0.3294511673884877 1103 3 0.3224742028289178 1103 4 0.22832587253324732 1103 5 0.216151201768497 1103 6 0.27276535428696747 1103 7 0.24533523138730118 1103 8 0.25110237921109047 1103 9 0.3147420718081264 1103 10-11 0.3669253776528123 1103 12-13 0.4332963261030969 1103 14-15 0.4878748431494344 1103 16-17 0.5411178872662319 1103 18-19 0.4377560942315242 1103 20-21 0.40991541776233476 1103 22-23 0.45418989950126587 1103 24-25 0.39659875513580545 1103 26-27 0.4203695969669283 1103 28-29 0.2798721641059956 1103 30-31 0.30849165766605324 1103 32-33 0.39020688424653116 1103 34-35 0.4470263541988615 1103 36-37 0.3961514139879512 1103 38-39 0.5219361887629859 1103 40-41 0.5117563270537389 1103 42-43 0.5262417817542371 1103 44-45 0.5021480302248804 1103 46-47 0.5952290728196772 1103 48-49 0.7933515883851285 1103 50-51 0.4994421530940727 1103 52-53 0.5990930609294125 1103 54-55 0.6809659705096678 1103 56-57 0.6348947297853726 1103 58-59 0.6735557403629926 1103 60-61 0.8661214052825201 1103 62-63 0.776533781938106 1103 64-65 0.8457711414581937 1103 66-67 0.9498708454777685 1103 68-69 0.8775482799576935 1103 70-71 0.7696658385593764 1103 72-73 0.7998726069662574 1103 74-75 0.8823368369773412 1103 76-77 0.9764480086351703 1103 78-79 1.071781776244741 1103 80-81 1.0990336362356814 1103 82-83 1.1692788969348413 1103 84-85 1.1608123695819046 1103 86-87 1.1603387902306963 1103 88-89 1.274364364698446 1103 90-91 1.3250444187327126 1103 92-93 1.2438376254365053 1103 94-95 1.3048509946285236 1103 96-97 1.511079670247625 1103 98-99 1.6380079321186543 1103 100-101 1.694755654020863 1104 1 0.2637602446572629 1104 2 0.22239748521355907 1104 3 0.19775326054422138 1104 4 0.09596722055364637 1104 5 0.15847022050240867 1104 6 0.0835244652410978 1104 7 0.2005678784933025 1104 8 0.2625600995112407 1104 9 0.38773823053157486 1104 10-11 0.2368430484033084 1104 12-13 0.35466451909430674 1104 14-15 0.364457653425319 1104 16-17 0.3702920336119817 1104 18-19 0.33333482375785195 1104 20-21 0.3633926421684208 1104 22-23 0.29247817831442546 1104 24-25 0.24117181358986528 1104 26-27 0.3025551742193997 1104 28-29 0.3084413983887231 1104 30-31 0.4179829485688913 1104 32-33 0.3865585036712176 1104 34-35 0.4169374718027683 1104 36-37 0.3816858340726128 1104 38-39 0.6019182675828887 1104 40-41 0.5847742140233976 1104 42-43 0.5388892968981338 1104 44-45 0.6080763510521479 1104 46-47 0.6829852407522097 1104 48-49 0.7200540080333795 1104 50-51 0.7672037808263426 1104 52-53 0.6113158350901884 1104 54-55 0.8007332771733573 1104 56-57 0.7398226266806915 1104 58-59 0.6949132772674957 1104 60-61 0.745548246508676 1104 62-63 0.7376323620665559 1104 64-65 0.7318606373305201 1104 66-67 0.7025864717389894 1104 68-69 0.5701999835677753 1104 70-71 0.6333750674701477 1104 72-73 0.7403861009006114 1104 74-75 0.8080880101870633 1104 76-77 0.6661898608895669 1104 78-79 0.656057264055427 1104 80-81 0.8466704005207326 1104 82-83 0.9581471465581828 1104 84-85 0.8739292495167028 1104 86-87 0.9270744517841365 1104 88-89 1.0697316136667006 1104 90-91 0.9833386797481225 1104 92-93 0.9561322415007076 1104 94-95 1.13172986381662 1104 96-97 1.05529208604489 1104 98-99 1.200383948203104 1104 100-101 1.2763672357308593 1105 1 0.2428886474846479 1105 2 0.28670363982833535 1105 3 0.24753585751785678 1105 4 0.1602131312437578 1105 5 0.23610483644679192 1105 6 0.17658534333745735 1105 7 0.22407780168170177 1105 8 0.17537938634723815 1105 9 0.3107339688405588 1105 10-11 0.2901976329301803 1105 12-13 0.22483259845118653 1105 14-15 0.20311303049980012 1105 16-17 0.25620507144707716 1105 18-19 0.3213828466357498 1105 20-21 0.33256522944099487 1105 22-23 0.38801353088095425 1105 24-25 0.28660473955498134 1105 26-27 0.2806771456201176 1105 28-29 0.30745596286452326 1105 30-31 0.4089982531502443 1105 32-33 0.2968152167531386 1105 34-35 0.3304821909276967 1105 36-37 0.3803839688252708 1105 38-39 0.42998622225273664 1105 40-41 0.323507526456261 1105 42-43 0.2200359189198764 1105 44-45 0.1693398892339104 1105 46-47 0.18864966085731538 1105 48-49 0.13089908356374735 1105 50-51 0.20672096811981078 1105 52-53 0.27426509372010344 1105 54-55 0.31309741817610615 1105 56-57 0.4323795076821142 1105 58-59 0.39668431742835963 1105 60-61 0.4486882552983289 1105 62-63 0.4175395697212849 1105 64-65 0.4057355194075072 1105 66-67 0.5041320683972046 1105 68-69 0.5203501639177972 1105 70-71 0.6137110925736096 1105 72-73 0.5521667824753216 1105 74-75 0.4749221072463463 1105 76-77 0.4790386493544183 1105 78-79 0.5672864095174255 1105 80-81 0.6244295484115767 1105 82-83 0.7134694885365782 1105 84-85 0.6053181714105129 1105 86-87 0.6631156958486208 1105 88-89 0.7843797032543094 1105 90-91 0.9069082861751632 1105 92-93 0.7883268453629064 1105 94-95 0.8742737536034042 1105 96-97 1.3172326108565606 1105 98-99 1.4831057043003852 1105 100-101 1.5458488683722358 1106 1 0.1933268013819358 1106 2 0.29887599599605963 1106 3 0.19114294713693525 1106 4 0.2478528448679569 1106 5 0.2529502087938482 1106 6 0.12302009689068427 1106 7 0.2716343739454956 1106 8 0.22729851247846966 1106 9 0.33714560076894884 1106 10-11 0.2508931042454776 1106 12-13 0.13910233972216446 1106 14-15 0.23939870407123465 1106 16-17 0.3011935173352782 1106 18-19 0.34239131756642394 1106 20-21 0.2978705077023349 1106 22-23 0.3880130841725915 1106 24-25 0.2841657118961365 1106 26-27 0.3085079700159312 1106 28-29 0.41591049934429236 1106 30-31 0.4510301597417481 1106 32-33 0.5147923694072389 1106 34-35 0.6191196723502159 1106 36-37 0.48587213842435517 1106 38-39 0.5189111158356354 1106 40-41 0.6007470206573231 1106 42-43 0.5700605102152281 1106 44-45 0.4652401785669156 1106 46-47 0.6487849598861217 1106 48-49 0.7555097213873552 1106 50-51 0.8003856607822186 1106 52-53 0.7653559421398981 1106 54-55 0.7367335170362423 1106 56-57 0.8370177699508474 1106 58-59 0.7465637285059259 1106 60-61 0.7689167287573682 1106 62-63 0.7505541766380261 1106 64-65 0.8139248898033387 1106 66-67 0.8014819263662858 1106 68-69 0.7255034362575827 1106 70-71 0.7060008168964771 1106 72-73 1.0531720996449572 1106 74-75 0.9702705330684118 1106 76-77 1.0702844162342977 1106 78-79 1.0478999366507118 1106 80-81 1.0308240446071792 1106 82-83 0.8543957025893967 1106 84-85 1.0961360810208625 1106 86-87 1.249481173869004 1106 88-89 1.2891096747498487 1106 90-91 1.3789867799817728 1106 92-93 1.3271672440590514 1106 94-95 1.5187521925542278 1106 96-97 1.7624462871011026 1106 98-99 1.7368889890988548 1106 100-101 1.9650087260866869 1107 1 0.1951681273611925 1107 2 0.15565535967197874 1107 3 0.12828337163368175 1107 4 0.07066825848797009 1107 5 0.13339313751263404 1107 6 0.09087221071981588 1107 7 0.13548384928922275 1107 8 0.09776291397735548 1107 9 0.3384063663975283 1107 10-11 0.24843285695764195 1107 12-13 0.25050840917421624 1107 14-15 0.28032937833524585 1107 16-17 0.39590157641115553 1107 18-19 0.46863021277084016 1107 20-21 0.3945412911547308 1107 22-23 0.21609143409002485 1107 24-25 0.28298190209163465 1107 26-27 0.2792100223271774 1107 28-29 0.2735595340048178 1107 30-31 0.25720557223899476 1107 32-33 0.21270450410097652 1107 34-35 0.3025063367713656 1107 36-37 0.26410416608167253 1107 38-39 0.21672407341871036 1107 40-41 0.333937669939921 1107 42-43 0.21647188938521822 1107 44-45 0.20417397784554225 1107 46-47 0.32397477645525186 1107 48-49 0.2873600762262143 1107 50-51 0.16755546415892297 1107 52-53 0.12535131075595274 1107 54-55 0.21350619093535528 1107 56-57 0.2865688999617504 1107 58-59 0.3594644718664384 1107 60-61 0.3412605832851341 1107 62-63 0.4646766229443813 1107 64-65 0.5046333512014556 1107 66-67 0.5063900238031138 1107 68-69 0.6313637743018177 1107 70-71 0.6170129848051928 1107 72-73 0.5296954273177086 1107 74-75 0.40360807675078547 1107 76-77 0.5606567514342196 1107 78-79 0.7510406231247337 1107 80-81 0.7687307526288336 1107 82-83 0.8731064937139834 1107 84-85 0.7834318206154549 1107 86-87 0.7416139027833388 1107 88-89 0.8391938817306084 1107 90-91 1.0248564275755605 1107 92-93 0.9715312240552159 1107 94-95 1.2296698615178734 1107 96-97 1.3621382298964946 1107 98-99 1.5513569179346014 1107 100-101 1.481655978366124 1108 1 0.08099285539697476 1108 2 0.0929075547131184 1108 3 0.13108860659971455 1108 4 0.16026275109753385 1108 5 0.12920001070644105 1108 6 0.297301794536331 1108 7 0.3472238680644182 1108 8 0.2127370520670695 1108 9 0.2401587178986233 1108 10-11 0.2744365015542556 1108 12-13 0.38491395337874934 1108 14-15 0.3266893834892173 1108 16-17 0.2884669674690059 1108 18-19 0.3217124738932995 1108 20-21 0.32291484390014347 1108 22-23 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-0.9654253108179951 2316 74-75 -0.9830014648124283 2316 76-77 -1.0735885471018811 2316 78-79 -1.0394107644043409 2316 80-81 -0.9790172703152002 2316 82-83 -1.1808271231561989 2316 84-85 -1.2617406120837096 2316 86-87 -1.3087969123459366 2316 88-89 -1.3753443004483437 2316 90-91 -1.3406677370220201 2316 92-93 -1.3324944248029347 2316 94-95 -1.2041938632581797 2316 96-97 -1.3998730541390252 2316 98-99 -1.560353858815379 2316 100-101 -1.4201419742158627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9486.0 3 1655.0 4 1408.0 5 1676.0 6 1942.0 7 2366.0 8 2969.0 9 3838.0 10 5124.0 11 7032.0 12 10344.0 13 14025.0 14 16126.0 15 20985.0 16 27568.0 17 37602.0 18 31552.0 19 19995.0 20 18667.0 21 19237.0 22 20838.0 23 23378.0 24 25771.0 25 30159.0 26 34737.0 27 40650.0 28 48461.0 29 58596.0 30 71681.0 31 89404.0 32 112930.0 33 147130.0 34 198527.0 35 278887.0 36 405009.0 37 560541.0 38 442689.0 39 48780.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80293112155705 19.371229611459206 15.020888021888371 21.80495124509537 2 14.77182690697309 20.79763406103271 41.363362274175266 23.067176757818935 3 18.570885181010183 25.638893637294473 31.301046564001705 24.489174617693635 4 11.837000769780232 17.1894352864484 38.98194830683024 31.991615636941127 5 12.558813411677235 38.17850425287548 35.052481260160576 14.210201075286715 6 29.862225833219284 37.494079677920624 18.192738315700847 14.450956173159243 7 26.723111068918897 34.32055024803708 21.61557058922579 17.34076809381823 8 25.561385194211507 34.75039402898404 20.591682722907453 19.096538053896996 9 26.151073316469414 16.79793891085325 21.53579690677251 35.51519086590482 10-11 23.98478480892482 27.249924897689343 28.102695609901794 20.66259468348404 12-13 25.437355612190455 25.129541936386907 28.567167095585944 20.865935355836697 14-15 22.3108522829242 26.021795061014945 28.067775752145796 23.59957690391506 16-17 20.63977464964412 29.47316905001347 28.435566481213442 21.45148981912897 18-19 20.468167321104723 27.573273704894312 31.711988818974763 20.246570155026202 20-21 21.572200855666885 26.410638691116173 31.679404320876987 20.33775613233996 22-23 21.123799513465613 26.53774175153766 31.573012376764137 20.765446358232595 24-25 20.94376169473287 26.611105895167388 31.883016121192508 20.562116288907234 26-27 20.856908424918576 26.779877915596135 31.802309941819285 20.560903717666 28-29 20.428910505573686 27.13973217000298 31.901518139877172 20.529839184546166 30-31 20.670045537664077 26.743593586093212 31.752078300181303 20.834282576061412 32-33 20.57184139790768 26.929577074369526 31.616261452810136 20.88232007491266 34-35 21.10935826023564 26.74996153968758 31.468885953739523 20.67179424633726 36-37 21.027564060372807 26.613244051227515 31.51867337783582 20.840518510563854 38-39 20.778941417802656 27.01597343603109 31.517417536625565 20.687667609540693 40-41 20.86713009584164 27.008276807652642 31.439571475237994 20.685021621267722 42-43 20.768452471378165 27.085999141167438 30.920778326101043 21.224770061353347 44-45 20.930801795063054 27.0921586038614 30.79564545114073 21.181394149934814 46-47 21.214236739196487 27.408692145360835 30.215843290405353 21.161227825037322 48-49 21.315987046584553 27.489785517521653 29.94474322698307 21.249484208910722 50-51 21.093217709634217 27.73052804157076 29.99619403591396 21.180060212881067 52-53 21.327808652387816 28.053662388348478 29.39191255603478 21.226616403228935 54-55 21.155743876830403 27.689896248349893 29.563374214223764 21.590985660595937 56-57 21.305798060541274 28.065534452661723 29.284974624511527 21.343692862285476 58-59 21.521462698034146 27.616967313822705 29.46309222328563 21.398477764857518 60-61 21.381994478159775 27.646453286208455 29.939063019014707 21.032489216617062 62-63 21.212211484404143 27.716063901313838 29.904093991836888 21.16763062244513 64-65 21.092461207131706 27.803932673298192 29.847010747436542 21.256595372133564 66-67 21.06587402324554 27.825840127036315 29.456865722900048 21.6514201268181 68-69 20.99702346156611 27.856485294410565 29.622333824371243 21.52415741965208 70-71 21.25790080602337 27.82417335754856 29.396367508414766 21.521558328013306 72-73 21.291156120920324 27.614949645396216 29.519210952451054 21.57468328123241 74-75 20.911068324235686 28.19660478811577 29.167518930652182 21.72480795699636 76-77 21.058061892410183 27.95377329209759 29.18353646370257 21.804628351789653 78-79 21.145923977807463 27.938318571563176 29.149634743184 21.766122707445366 80-81 21.080904892236674 28.196282479979313 29.025011331156758 21.697801296627254 82-83 21.22861478574692 28.04012008383839 29.12634054305134 21.604924587363346 84-85 21.17067182842745 27.85494306886583 28.9727492212418 22.001635881464924 86-87 21.170723023241596 27.91249900208104 29.070414027701364 21.846363946976 88-89 21.265956334007367 27.64407373771171 29.022343543585983 22.067626384694943 90-91 21.13298072989535 27.503968780428327 29.33840612115319 22.024644368523127 92-93 21.31345082612389 27.47786510987132 29.355856296613158 21.852827767391638 94-95 21.055961388285656 27.412630310465936 29.392644508597943 22.13876379265047 96-97 20.910306688031124 27.47679455552715 29.462237767566616 22.150660988875103 98-99 20.923070368337584 27.64394570634797 28.99628169088241 22.436702234432037 100-101 21.09850161350314 26.898576141486807 29.706223987067037 22.296698257943014 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3970.0 1 2350.0 2 812.0 3 876.5 4 759.0 5 765.5 6 1333.0 7 2908.5 8 4922.5 9 5796.5 10 5287.0 11 4741.5 12 4825.5 13 5192.0 14 5587.5 15 5889.0 16 6172.5 17 6447.5 18 6703.5 19 7319.0 20 8260.5 21 9375.0 22 10926.0 23 12938.5 24 15430.0 25 18730.5 26 23197.0 27 27881.5 28 31873.5 29 36779.5 30 42360.5 31 47270.0 32 53734.0 33 62119.0 34 69441.5 35 75219.0 36 82255.5 37 88438.0 38 90900.5 39 91952.5 40 93865.0 41 97543.0 42 101109.5 43 103901.0 44 105587.0 45 106314.5 46 106034.5 47 110422.5 48 124070.5 49 120764.0 50 102361.5 51 90761.0 52 83387.0 53 79146.0 54 74388.5 55 67369.0 56 59842.0 57 52789.0 58 46630.0 59 40662.0 60 34744.0 61 28692.0 62 22393.5 63 16688.5 64 11937.5 65 8778.5 66 6264.5 67 4542.5 68 3168.0 69 1957.0 70 1403.0 71 1099.5 72 887.0 73 715.0 74 509.0 75 384.5 76 290.0 77 195.5 78 122.5 79 78.0 80 51.5 81 35.0 82 31.5 83 26.5 84 15.5 85 9.5 86 7.5 87 7.0 88 4.5 89 2.5 90 6.0 91 5.5 92 2.5 93 3.5 94 2.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30344768440887526 2 0.2710453504725657 3 0.26827887585687227 4 0.2706649602129079 5 0.2791027077907729 6 0.1909559103482404 7 0.1903680344924055 8 0.1922008239253024 9 0.19109423407902504 10-11 0.19724964009894297 12-13 0.18822401666524308 14-15 0.18760155987671204 16-17 0.19346302796871256 18-19 0.188414211795072 20-21 0.18142886339044603 22-23 0.1808236970682631 24-25 0.1839014000782221 26-27 0.1783511603804871 28-29 0.19263308558400452 30-31 0.19692112123832933 32-33 0.19847726320965692 34-35 0.1962467930507541 36-37 0.17418415799059883 38-39 0.16768294264371922 40-41 0.17380376773094097 42-43 0.18295042442907744 44-45 0.18694452215548488 46-47 0.18881189252107794 48-49 0.1875669789440159 50-51 0.19045448682414592 52-53 0.21338164520170538 54-55 0.18018394981338398 56-57 0.1669394525907516 58-59 0.16695674305709968 60-61 0.1620462506142438 62-63 0.16868578969190812 64-65 0.16597118647525888 66-67 0.16317013092686927 68-69 0.17375189633189672 70-71 0.16868578969190812 72-73 0.16398278284522924 74-75 0.16766565217737114 76-77 0.17121019777872837 78-79 0.17397667239442183 80-81 0.1665244813983976 82-83 0.1654351820184683 84-85 0.1604036563111758 86-87 0.15590813506067397 88-89 0.16145837475840896 90-91 0.16806333290337708 92-93 0.160438237243872 94-95 0.1648645966289815 96-97 0.16353323072017906 98-99 0.1672506809850171 100-101 0.14734935421837236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2891767.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.10924737548425 #Duplication Level Percentage of deduplicated Percentage of total 1 66.4118166102506 27.96551614296337 2 15.748185180776545 13.262884509845097 3 6.579099595275702 8.311227970962385 4 3.468809505025214 5.842758301821513 5 2.086995094985068 4.3940896363074256 6 1.3259291834491946 3.3500327992941585 7 0.9015700697349943 2.657510596496243 8 0.6433816577365105 2.167385390598069 9 0.45645959334933905 1.729905293993424 >10 2.147806828753081 15.782608291002848 >50 0.1408056869624454 4.135472509189688 >100 0.08323246065661927 6.032614558429923 >500 0.0033645813171841155 0.9440379426417119 >1k 0.0022156998917300806 1.6873049768332469 >5k 8.206295895296593E-5 0.20858058573288846 >10k+ 2.461888768588978E-4 1.5280704938880598 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19208 0.6642305552279972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14792 0.5115211564417189 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10220 0.3534171321548382 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6036 0.2087305097540708 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4686 0.1620462506142438 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3761 0.1300588878702883 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3573 0.1235576725234087 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3301 0.11415165883005096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 1.729046634808406E-5 0.0 0.0 0.0 0.0 18-19 3.458093269616812E-5 0.0 0.0 0.0 0.0 20-21 3.458093269616812E-5 0.0 0.0 5.187139904425218E-5 0.0 22-23 3.458093269616812E-5 0.0 0.0 1.5561419713275656E-4 0.0 24-25 3.458093269616812E-5 0.0 0.0 3.1122839426551307E-4 0.0 26-27 3.458093269616812E-5 0.0 0.0 6.743281875752783E-4 0.0 28-29 3.458093269616812E-5 0.0 0.0 0.0017982085002007423 0.0 30-31 3.458093269616812E-5 0.0 0.0 0.003752031197534241 0.0 32-33 3.458093269616812E-5 0.0 0.0 0.00643205348148727 0.0 34-35 3.458093269616812E-5 0.0 0.0 0.010114922813629176 0.0 36-37 3.458093269616812E-5 0.0 0.0 0.014593153597782947 0.0 38-39 3.458093269616812E-5 0.0 0.0 0.018915770184803962 0.0 40-41 3.458093269616812E-5 0.0 0.0 0.024708076411412122 0.0 42-43 3.458093269616812E-5 0.0 0.0 0.03423512336920644 0.0 44-45 3.458093269616812E-5 0.0 0.0 0.047324006394706075 0.0 46-47 3.458093269616812E-5 0.0 0.0 0.07542101421034267 0.0 48-49 3.458093269616812E-5 0.0 0.0 0.12532130009091327 0.0 50-51 3.458093269616812E-5 0.0 0.0 0.16365426398461563 0.0 52-53 3.458093269616812E-5 0.0 0.0 0.20205638974371032 0.0 54-55 3.458093269616812E-5 0.0 0.0 0.25271745614359664 0.0 56-57 3.458093269616812E-5 0.0 0.0 0.2969810499946918 0.0 58-59 3.458093269616812E-5 0.0 0.0 0.3504431719429677 0.0 60-61 3.458093269616812E-5 0.0 0.0 0.4296507982835408 0.0 62-63 3.458093269616812E-5 0.0 0.0 0.4910492442855873 0.0 64-65 3.458093269616812E-5 0.0 0.0 0.5624415798368264 0.0 66-67 3.458093269616812E-5 0.0 0.0 0.6804490126625001 0.0 68-69 3.458093269616812E-5 0.0 0.0 0.8113032619848003 0.0 70-71 3.458093269616812E-5 0.0 0.0 0.9286363666228987 0.0 72-73 3.458093269616812E-5 0.0 0.0 1.0703144478790994 0.0 74-75 3.458093269616812E-5 0.0 0.0 1.2075315888174947 0.0 76-77 3.458093269616812E-5 0.0 0.0 1.3247782411238527 0.0 78-79 3.458093269616812E-5 0.0 0.0 1.4822598086222023 0.0 80-81 3.458093269616812E-5 0.0 0.0 1.6962466201460906 0.0 82-83 3.458093269616812E-5 0.0 0.0 1.9012596796353234 0.0 84-85 3.458093269616812E-5 0.0 0.0 2.1316897246562396 0.0 86-87 3.458093269616812E-5 0.0 0.0 2.4986625824279756 0.0 88-89 3.458093269616812E-5 0.0 0.0 2.956116450599236 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16570 0.0 27.952978 1 GTATCAA 23190 0.0 27.663225 1 GTACATG 14000 0.0 26.868292 1 TACATGG 14315 0.0 25.970871 2 ACATGGG 14340 0.0 24.76524 3 GAGTACT 14980 0.0 23.11688 12-13 ATCAACG 27895 0.0 22.820509 3 TCAACGC 27960 0.0 22.786434 4 TATCAAC 28090 0.0 22.729788 2 CAACGCA 28135 0.0 22.627806 5 AACGCAG 28660 0.0 22.31047 6 CATGGGA 9850 0.0 21.962608 4 AGTACTT 15535 0.0 21.755907 12-13 GTACTTT 16245 0.0 21.198885 14-15 CATGGGG 4505 0.0 20.474611 4 ACGCAGA 31420 0.0 20.093296 7 AGAGTAC 24300 0.0 20.06625 10-11 CGCAGAG 31580 0.0 19.901237 8 ATGGGAG 3960 0.0 19.810595 5 GCAGAGT 32195 0.0 19.107931 9 >>END_MODULE