FastQCFastQC Report
Thu 26 May 2016
SRR937783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891767
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200160.6921719488465011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171180.5919564058930059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115180.3983031827944644No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82910.2867105129839299No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37860.13092341118769252No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33090.11442830629162032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.10263620824222698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG146600.024.958831
GTATCAA281250.023.856661
GGTATCA211100.023.7482551
TACATGG150950.023.6713032
GAGTACT162300.022.50738712-13
AGTACTT166050.022.4997212-13
ACATGGG152200.022.4778753
GTACTTT168200.021.67327714-15
AGAGTAC264100.020.19894210-11
ATCAACG334600.019.9520633
TCAACGC334500.019.9296194
CAACGCA336000.019.8265045
TATCAAC339300.019.7036952
AACGCAG340700.019.5594986
TACTTTT195150.019.00879914-15
CATGGGG49400.018.9485824
CATGGGA104250.018.8239784
GTGTAGC13250.018.6489731
ACTTTTT199900.018.43830916-17
ACGCAGA371150.017.8414677
Produced by FastQC (version 0.11.5)