##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937783_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2891767 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14791751894257 33.0 31.0 34.0 31.0 34.0 2 32.42812439591433 34.0 31.0 34.0 31.0 34.0 3 32.51221934547286 34.0 31.0 34.0 31.0 34.0 4 36.14976829046047 37.0 35.0 37.0 35.0 37.0 5 36.070999496155814 37.0 35.0 37.0 35.0 37.0 6 36.11462230532405 37.0 35.0 37.0 35.0 37.0 7 36.058570071516826 37.0 35.0 37.0 35.0 37.0 8 36.043359994079744 37.0 35.0 37.0 35.0 37.0 9 37.731842849026215 39.0 37.0 39.0 35.0 39.0 10-11 37.65301837941992 39.0 37.0 39.0 35.0 39.0 12-13 37.55722729390023 39.0 37.0 39.0 35.0 39.0 14-15 38.75759405927241 40.0 38.0 41.0 35.0 41.0 16-17 38.72877206220279 40.0 38.0 41.0 34.5 41.0 18-19 38.74085948141742 40.0 38.0 41.0 35.0 41.0 20-21 38.66505081495155 40.0 38.0 41.0 34.0 41.0 22-23 38.605366027069266 40.0 38.0 41.0 34.0 41.0 24-25 38.620454379623254 40.0 38.0 41.0 34.0 41.0 26-27 38.484330341967386 40.0 38.0 41.0 34.0 41.0 28-29 38.33877815882123 40.0 38.0 41.0 34.0 41.0 30-31 38.34710818679375 40.0 38.0 41.0 34.0 41.0 32-33 38.237746332951446 40.0 38.0 41.0 34.0 41.0 34-35 38.07565754778999 40.0 37.0 41.0 34.0 41.0 36-37 37.91747571640454 40.0 37.0 41.0 33.0 41.0 38-39 37.782065775008846 40.0 37.0 41.0 33.0 41.0 40-41 37.59807636645691 40.0 37.0 41.0 33.0 41.0 42-43 37.354722389459454 40.0 37.0 41.0 33.0 41.0 44-45 37.018578260281686 40.0 36.5 41.0 31.5 41.0 46-47 36.584615565500265 40.0 36.0 41.0 30.5 41.0 48-49 36.20106270664269 39.5 36.0 41.0 30.0 41.0 50-51 35.92749208355998 39.0 35.5 41.0 29.0 41.0 52-53 35.701324138493874 39.0 35.0 41.0 28.0 41.0 54-55 35.4837635604805 39.0 35.0 40.0 28.0 41.0 56-57 35.22698267184043 39.0 35.0 40.0 27.0 41.0 58-59 34.98011700112768 38.0 35.0 40.0 26.5 41.0 60-61 34.663559339324365 38.0 34.0 40.0 26.0 41.0 62-63 34.31957692303702 38.0 34.0 40.0 26.0 41.0 64-65 33.92940561947073 37.0 34.0 40.0 24.5 41.0 66-67 33.513232739705515 36.5 33.0 39.0 23.0 41.0 68-69 33.078385810475055 36.0 33.0 39.0 22.0 40.0 70-71 32.68563667128092 35.5 32.0 39.0 21.5 40.0 72-73 32.20444109086244 35.0 32.0 38.0 20.5 40.0 74-75 31.72954546476255 35.0 31.0 37.0 18.5 39.0 76-77 30.469164528124153 34.0 29.5 36.0 18.0 38.5 78-79 31.05345849095034 34.0 31.0 36.0 18.0 39.0 80-81 30.85392650929345 34.0 31.0 36.0 17.0 37.5 82-83 30.47933339719279 34.0 31.0 35.5 12.0 37.0 84-85 30.097459269712946 34.0 30.0 35.0 7.0 37.0 86-87 29.738243606763618 34.0 30.0 35.0 4.5 36.0 88-89 29.315477180561228 34.0 29.5 35.0 2.0 36.0 90-91 28.789683608672483 34.0 29.0 35.0 2.0 35.5 92-93 28.370447031175058 34.0 29.0 35.0 2.0 35.0 94-95 27.92891768251038 33.5 28.5 35.0 2.0 35.0 96-97 27.393952036937968 33.0 27.0 35.0 2.0 35.0 98-99 26.562209541778437 33.0 25.5 34.5 2.0 35.0 100-101 23.868050226729885 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30362132726141766 1101 2 0.2757947990176177 1101 3 0.28439940586496704 1101 4 0.13353703073114787 1101 5 0.05146548330198897 1101 6 0.12059198526686288 1101 7 0.1280667676941647 1101 8 0.07794730947508555 1101 9 0.14404252502697545 1101 10-11 0.2202149626735732 1101 12-13 0.21859173292848055 1101 14-15 0.25637557768187236 1101 16-17 0.26499937529743534 1101 18-19 0.3145313306699009 1101 20-21 0.2985105892823867 1101 22-23 0.22771576214006473 1101 24-25 0.182293975758256 1101 26-27 0.12364685494657124 1101 28-29 0.06225415757399588 1101 30-31 0.08995349902542671 1101 32-33 0.216541383825529 1101 34-35 0.2065296615096699 1101 36-37 0.14279725779094576 1101 38-39 -9.684571759436267E-4 1101 40-41 0.030349068549362812 1101 42-43 0.18294165977376053 1101 44-45 0.09545818077432244 1101 46-47 0.08962870244727839 1101 48-49 0.13606823745383423 1101 50-51 0.20308216420215786 1101 52-53 0.25273127458149247 1101 54-55 0.08724546076553708 1101 56-57 0.08642053450982701 1101 58-59 -0.013106545403005043 1101 60-61 0.15064334254338263 1101 62-63 0.002095264705495481 1101 64-65 0.13335716020727517 1101 66-67 0.11509067428640662 1101 68-69 0.037858455909137945 1101 70-71 0.021272063917059825 1101 72-73 -0.10993340855139877 1101 74-75 -0.03581408566078892 1101 76-77 -0.1068911068073426 1101 78-79 -0.04642293626625005 1101 80-81 0.021953907671214523 1101 82-83 0.050468715981164536 1101 84-85 -0.08211156920556206 1101 86-87 0.02196969252540626 1101 88-89 -0.017525180671874807 1101 90-91 -0.12653645774954114 1101 92-93 -0.2812595400836919 1101 94-95 -0.40675828317914764 1101 96-97 -0.444848080194415 1101 98-99 -0.5785127965947297 1101 100-101 -0.5262627957603847 1102 1 0.37626652211348954 1102 2 0.2507006284350197 1102 3 0.26236181580853213 1102 4 0.11870842566168704 1102 5 0.17678913133011775 1102 6 0.10003808738338904 1102 7 0.07202609673097271 1102 8 0.15090063658617225 1102 9 0.2607270343949608 1102 10-11 0.21826953992421494 1102 12-13 0.3092236805884099 1102 14-15 0.3749735426367167 1102 16-17 0.309487997378632 1102 18-19 0.2752802812633135 1102 20-21 0.24608901694708862 1102 22-23 0.26095168312597394 1102 24-25 0.2398103341090092 1102 26-27 0.21266225178743525 1102 28-29 0.11896265634822356 1102 30-31 0.2778983233910708 1102 32-33 0.3465361879921005 1102 34-35 0.2931386847116926 1102 36-37 0.24083612694971634 1102 38-39 0.28264956236530026 1102 40-41 0.3352441715543222 1102 42-43 0.30929480637173157 1102 44-45 0.3339845164143469 1102 46-47 0.33709523984533263 1102 48-49 0.27456490340873785 1102 50-51 0.33413308025108535 1102 52-53 0.3795230730239041 1102 54-55 0.3094783675858892 1102 56-57 0.37286377548734606 1102 58-59 0.38946669162618264 1102 60-61 0.431617807693776 1102 62-63 0.34240145918269604 1102 64-65 0.3438429404856791 1102 66-67 0.34809989351211357 1102 68-69 0.3193181981799924 1102 70-71 0.2096908859328508 1102 72-73 0.24701780155384512 1102 74-75 0.3329311881371808 1102 76-77 0.27006993566937254 1102 78-79 0.35603215220949735 1102 80-81 0.4762501175820546 1102 82-83 0.525113402269465 1102 84-85 0.5392211735062915 1102 86-87 0.23900588226419117 1102 88-89 0.1311355355630539 1102 90-91 0.3256585999504047 1102 92-93 0.2258739712123088 1102 94-95 0.22090816801671664 1102 96-97 0.1562792326947644 1102 98-99 0.1588277912226559 1102 100-101 0.18402795883722334 1103 1 0.29963959889418845 1103 2 0.25753352086003645 1103 3 0.23812989328689582 1103 4 0.15061613961137965 1103 5 0.1451448233188799 1103 6 0.1879857706925634 1103 7 0.1973631851413984 1103 8 0.16981175915109503 1103 9 0.2267818399159509 1103 10-11 0.24231992592070384 1103 12-13 0.30796502734737885 1103 14-15 0.2536274101478 1103 16-17 0.2674697668400583 1103 18-19 0.272975835255842 1103 20-21 0.20708642844981995 1103 22-23 0.18193346113029207 1103 24-25 0.21751337737307352 1103 26-27 0.22764221869796586 1103 28-29 0.28124349548834715 1103 30-31 0.19397932712432464 1103 32-33 0.18574323645025004 1103 34-35 0.2117142138728667 1103 36-37 0.31587976690654784 1103 38-39 0.32596560108170536 1103 40-41 0.2538118697281391 1103 42-43 0.2876855527075435 1103 44-45 0.3905463142951433 1103 46-47 0.30557836335565725 1103 48-49 0.2993998119570378 1103 50-51 0.17072030748355616 1103 52-53 0.24605386019475617 1103 54-55 0.18541559439120903 1103 56-57 0.12719431149707106 1103 58-59 0.1551806356246459 1103 60-61 0.12477480617598502 1103 62-63 0.197088233926479 1103 64-65 0.06383032547694967 1103 66-67 0.03937674312013684 1103 68-69 0.14310318489579288 1103 70-71 0.14749860814067262 1103 72-73 0.07595415413621964 1103 74-75 0.014764578897754177 1103 76-77 -0.0012509952784007794 1103 78-79 0.04957785242791246 1103 80-81 0.035217452398054405 1103 82-83 -0.059143750269626594 1103 84-85 -0.0556854099335915 1103 86-87 -0.09782980083995696 1103 88-89 0.02861936813369681 1103 90-91 0.04053590973572696 1103 92-93 0.010940420699590447 1103 94-95 -0.08381690737433445 1103 96-97 -0.01371085754668755 1103 98-99 0.20815660441516215 1103 100-101 0.2667238972600323 1104 1 0.2790026191758841 1104 2 0.1960648902064719 1104 3 0.1682415672263602 1104 4 0.08906891742896761 1104 5 0.066699497827436 1104 6 0.10428694822481788 1104 7 0.1078757498464995 1104 8 0.09031689002216581 1104 9 0.16507188703850773 1104 10-11 0.09152266109082774 1104 12-13 0.13799824844893038 1104 14-15 0.10709754975871277 1104 16-17 0.1399461697335127 1104 18-19 0.1760850779432488 1104 20-21 0.0941553134625508 1104 22-23 0.1340994205502497 1104 24-25 0.11649460000761991 1104 26-27 0.07595958562285432 1104 28-29 0.1568250728018441 1104 30-31 0.15926290616404515 1104 32-33 0.17906058719452744 1104 34-35 0.22471729789692318 1104 36-37 0.253581937835726 1104 38-39 0.11392478942537565 1104 40-41 0.125429660168912 1104 42-43 0.2703060184964201 1104 44-45 0.20006448812388555 1104 46-47 0.22878467351574017 1104 48-49 0.07832692663549068 1104 50-51 0.052626538081277374 1104 52-53 0.16772688429969662 1104 54-55 0.04459390608798941 1104 56-57 0.16050429081825257 1104 58-59 0.14768022995367147 1104 60-61 0.12341037633209595 1104 62-63 0.11157920584017944 1104 64-65 0.10104728617610448 1104 66-67 0.04939496710876767 1104 68-69 0.10616337491161687 1104 70-71 -0.15569913091973575 1104 72-73 -0.0656963280690448 1104 74-75 -0.02445837278741081 1104 76-77 -0.0805111300027761 1104 78-79 0.026364688624209265 1104 80-81 -0.01399725642264471 1104 82-83 -0.1258280846201778 1104 84-85 -0.23103333925484293 1104 86-87 -0.15438854130437818 1104 88-89 -0.16036835217106926 1104 90-91 -0.1280099227635354 1104 92-93 -0.1345546319636668 1104 94-95 -0.12657410855431905 1104 96-97 0.005655771010793842 1104 98-99 0.22405029642790453 1104 100-101 -0.018299897481082184 1105 1 0.29723350443470764 1105 2 0.21096231806527044 1105 3 0.1717475250522753 1105 4 0.0652164985817123 1105 5 0.09245514751462025 1105 6 0.14359676519303832 1105 7 0.12929192823913382 1105 8 0.11174464002014162 1105 9 0.18322797439482486 1105 10-11 0.200924196750222 1105 12-13 0.2127344754947984 1105 14-15 0.14148752574226364 1105 16-17 0.13667826488121904 1105 18-19 0.2097001142542254 1105 20-21 0.059427315378904666 1105 22-23 0.07487642163869879 1105 24-25 0.0669600171317839 1105 26-27 0.09411227307451497 1105 28-29 0.013379814607112905 1105 30-31 0.036037716043765045 1105 32-33 0.1480671159658371 1105 34-35 0.18139451977636867 1105 36-37 0.224948716210136 1105 38-39 0.20378933960964218 1105 40-41 0.20217749747165925 1105 42-43 0.17184591285840156 1105 44-45 0.30759810423385403 1105 46-47 0.12706292600769586 1105 48-49 0.06441547112795121 1105 50-51 0.21550803720779754 1105 52-53 0.3313941192872747 1105 54-55 0.24492654798807934 1105 56-57 0.21703373017516014 1105 58-59 0.2831018920942938 1105 60-61 0.16559609511011786 1105 62-63 0.07273398286130828 1105 64-65 0.27305055056175576 1105 66-67 0.2839756240610143 1105 68-69 0.32896515164214435 1105 70-71 0.06801238872073867 1105 72-73 0.16205588878808896 1105 74-75 0.12926622873182936 1105 76-77 0.00881056873751973 1105 78-79 0.12686634753909587 1105 80-81 0.19557215102049952 1105 82-83 0.25083399157871966 1105 84-85 0.2620268588605832 1105 86-87 0.09419757553686381 1105 88-89 0.2433833889379997 1105 90-91 0.3713154049276248 1105 92-93 0.3598217467871443 1105 94-95 0.1861063548061601 1105 96-97 0.13331389998003473 1105 98-99 0.20860262084131875 1105 100-101 0.20475967552906482 1106 1 0.1906886462043076 1106 2 0.2012258625978447 1106 3 0.1637786945749653 1106 4 0.06659861425505653 1106 5 0.08541010993081244 1106 6 0.03356892186080529 1106 7 0.05452411607821972 1106 8 0.08008641837506758 1106 9 0.08860397552410149 1106 10-11 -0.002561959980972972 1106 12-13 -0.06349746799480727 1106 14-15 0.04610166575942998 1106 16-17 0.1978027102369495 1106 18-19 0.10326447621844181 1106 20-21 0.1986473268414457 1106 22-23 0.19932847799363884 1106 24-25 0.17303516151909548 1106 26-27 0.2582251080331872 1106 28-29 0.3392977891241955 1106 30-31 0.1267344783462292 1106 32-33 0.1174206887616549 1106 34-35 -0.16062170735160208 1106 36-37 0.21098282596727813 1106 38-39 0.30719830513581314 1106 40-41 0.07760438315050777 1106 42-43 0.0022787687945111657 1106 44-45 -0.010321110345635987 1106 46-47 0.012155017170151439 1106 48-49 -0.05808245949555868 1106 50-51 0.1330769430894705 1106 52-53 0.0770765712873498 1106 54-55 0.07269590531322478 1106 56-57 0.11520142181417015 1106 58-59 0.16056487448906864 1106 60-61 0.11153723592287434 1106 62-63 -0.05439407993873857 1106 64-65 -0.3761594233638732 1106 66-67 -0.4400477262785074 1106 68-69 -0.026120645399480225 1106 70-71 -0.0038292603121163893 1106 72-73 -0.06056904102285898 1106 74-75 -0.07200291439665563 1106 76-77 -0.09046500431113813 1106 78-79 -0.029564890419319312 1106 80-81 -0.2915758260383434 1106 82-83 -0.26516911215098204 1106 84-85 -0.41646194461766584 1106 86-87 -0.21287980373238113 1106 88-89 -0.10673322428001342 1106 90-91 -0.36452293488968124 1106 92-93 -0.43674346794333374 1106 94-95 -0.1728289515486665 1106 96-97 -0.0028923935154026026 1106 98-99 -0.09356454010815085 1106 100-101 -0.2786087020752994 1107 1 0.1915003079355131 1107 2 0.12880879477847884 1107 3 0.14613686428945982 1107 4 0.04309972064121581 1107 5 0.025183873842507865 1107 6 0.040395768917541375 1107 7 0.11009978774584539 1107 8 0.10043737435820788 1107 9 0.03928768942620309 1107 10-11 0.10995236690116172 1107 12-13 0.14255701392173847 1107 14-15 0.03624266567054946 1107 16-17 0.10954186507955654 1107 18-19 0.18195987617820464 1107 20-21 0.16536288033860558 1107 22-23 0.09642895095617376 1107 24-25 0.08745092880842265 1107 26-27 0.1352651390962123 1107 28-29 0.1483313268933628 1107 30-31 0.1411497778189954 1107 32-33 0.11155450301041014 1107 34-35 0.029704080100621866 1107 36-37 0.021745413741356856 1107 38-39 -0.04348704612424825 1107 40-41 -0.03004348196862594 1107 42-43 0.0785578962230602 1107 44-45 -0.058603287938154836 1107 46-47 -0.1707694444899417 1107 48-49 -0.12788069021333115 1107 50-51 -0.05234612681635298 1107 52-53 -0.07143577205258111 1107 54-55 -0.11829581901182706 1107 56-57 -0.24177746562448732 1107 58-59 -0.14285392892973903 1107 60-61 -0.08835633580978453 1107 62-63 -0.2455752699705087 1107 64-65 -0.07918011489031329 1107 66-67 -0.09071717177553751 1107 68-69 -0.07834394359979768 1107 70-71 -0.09489864218609512 1107 72-73 0.06036621784343055 1107 74-75 -0.1398117551939997 1107 76-77 -0.09801883830175129 1107 78-79 -0.06919947890448341 1107 80-81 -0.026335532062418565 1107 82-83 -0.06353128637522687 1107 84-85 0.13567667878532674 1107 86-87 -0.030892846458069556 1107 88-89 -0.14849169994653266 1107 90-91 -0.010842154197405307 1107 92-93 0.015833031759601823 1107 94-95 -0.06426744068830459 1107 96-97 0.04101312625218512 1107 98-99 -0.0033786298072939758 1107 100-101 0.108203783013046 1108 1 0.19167517494454955 1108 2 0.14494073653856532 1108 3 0.05602145206349718 1108 4 0.0876871265351582 1108 5 0.06652059407370103 1108 6 0.03846486899669088 1108 7 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-0.06479151010827167 2316 100-101 0.007992305253456067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 6.0 4 19.0 5 76.0 6 152.0 7 345.0 8 735.0 9 1379.0 10 2515.0 11 4247.0 12 6883.0 13 9947.0 14 12576.0 15 17680.0 16 25237.0 17 36585.0 18 32724.0 19 17887.0 20 13958.0 21 13944.0 22 14147.0 23 15751.0 24 17534.0 25 20864.0 26 25432.0 27 31310.0 28 39290.0 29 49124.0 30 62070.0 31 77931.0 32 100602.0 33 132635.0 34 183497.0 35 266854.0 36 416113.0 37 651481.0 38 543273.0 39 46956.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85959857042899 20.31227680246761 15.353984152694178 21.47414047440922 2 15.147451367969825 21.697737058345297 40.08390025890745 23.070911314777433 3 18.84947853682541 25.961946450042483 31.30594546517752 23.88262954795459 4 12.220905840929037 17.947614018561666 38.200151741185145 31.63132839932415 5 12.537079541779022 38.86513684188678 34.53888184533745 14.058901770996751 6 29.974614137307743 38.14052100324818 17.52368015818702 14.361184701257052 7 26.890202426405725 34.79713268738456 21.077908420699178 17.234756465510532 8 25.989023320343584 35.270891465322066 19.9306859785038 18.809399235830547 9 26.316677657639776 17.000366903695905 21.02662489751076 35.65633054115356 10-11 24.234767185599672 27.756264595314907 27.454252019612923 20.5547161994725 12-13 25.613582910595046 25.51161736343412 28.235067502679257 20.639732223291578 14-15 22.30935272447607 26.589209988218276 27.641905450888675 23.45953183641697 16-17 20.62180320890307 29.893383526404442 28.17901649752556 21.305796767166925 18-19 20.54588077116863 27.934650336628092 31.456216908208717 20.06325198399456 20-21 21.620033702577004 26.76598771616109 31.55311268162338 20.060865899638525 22-23 21.162787038508334 26.916810423305883 31.3903291449245 20.53007339326128 24-25 21.035525794343787 26.976686845856396 31.767976435095886 20.219810924703935 26-27 21.042208044269607 27.162477342758358 31.624054136348224 20.17126047662381 28-29 20.503084995943617 27.55218487981968 31.695869404489617 20.248860719747086 30-31 20.769308177318575 27.17386981731239 31.565354331797824 20.49146767357121 32-33 20.697103189848974 27.33539735393619 31.407336759842686 20.56016269637215 34-35 21.201569836020674 27.10553789430476 31.35518871333686 20.3377035563377 36-37 21.144805234999918 26.965000983827537 31.359597782255623 20.530595998916926 38-39 20.91003528292563 27.427590120504174 31.31531689793818 20.347057698632014 40-41 21.00324699520239 27.435959637596675 31.15439938542416 20.406393981776784 42-43 20.897849360099467 27.55699965242533 30.707011843477268 20.83813914399794 44-45 21.11029346416914 27.512970443331014 30.476175985132965 20.900560107366882 46-47 21.364584352750413 27.953911916139855 29.830636424027247 20.850867307082485 48-49 21.496873710779603 28.049562775977456 29.56503757045433 20.888525942788615 50-51 21.31476059957825 28.238073205093357 29.536293738843323 20.91087245648507 52-53 21.5730073641838 28.677626634100644 28.804227450591185 20.945138551124373 54-55 21.298326970426317 28.31832517221346 29.021789498436938 21.361558358923286 56-57 21.497478877101788 28.626960609205376 28.82210081240985 21.053459701282986 58-59 21.740081410431753 28.22117065448219 28.971628765388086 21.067119169697975 60-61 21.413637405779927 28.3591485759399 29.433370669213666 20.793843349066503 62-63 21.310568935878997 28.332192738903238 29.450004097840527 20.90723422737724 64-65 21.232346866120263 28.424160729408698 29.26909394844052 21.074398456030515 66-67 21.215557823296276 28.407648334046275 28.94012553570187 21.436668306955575 68-69 21.222664204965337 28.405780963680684 29.030969645894704 21.340585185459272 70-71 21.430685805599136 28.519759717847254 28.88941259790294 21.160141878650666 72-73 21.52253276284016 28.288949282566676 28.874802153838814 21.31371580075435 74-75 21.13251902124861 28.964152009533272 28.426228124958936 21.477100844259184 76-77 21.302736354623313 28.591273086662927 28.670601746267938 21.435388812445815 78-79 21.351668374388392 28.66906289476296 28.446465430997726 21.532803299850922 80-81 21.25881857009918 28.86835280989098 28.427791727341795 21.445036892668046 82-83 21.429514999567736 28.771193913720065 28.434200743494426 21.365090343217773 84-85 21.209039317483047 28.62462639624838 28.397204892372034 21.769129393896534 86-87 21.18322465122536 28.627773261123735 28.412783602551656 21.77621848509925 88-89 21.288108620092835 28.44046563917494 28.366133924344528 21.905291816387695 90-91 21.23938183945457 28.304454219241943 28.525104796054713 21.931059145248778 92-93 21.320872228896455 28.317059270648105 28.55376976593153 21.808298734523905 94-95 21.07771822556935 28.23540070828666 28.635450228182286 22.051430837961703 96-97 20.910450254117986 28.293669579879705 28.72952765558221 22.0663525104201 98-99 20.92481455346986 28.46875916944715 28.1667261176805 22.43970015940249 100-101 20.790480340887083 28.275936609390996 28.55577806369497 22.377804986026952 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1366.0 1 956.5 2 677.0 3 825.5 4 750.0 5 786.0 6 1380.5 7 2895.0 8 4725.5 9 5698.0 10 5465.0 11 5101.0 12 5319.0 13 5670.5 14 6133.5 15 6577.5 16 6862.0 17 7144.0 18 7528.0 19 7984.0 20 8678.5 21 9852.0 22 11497.5 23 13443.5 24 15701.5 25 19052.0 26 23442.5 27 27995.5 28 31725.5 29 36595.5 30 42624.0 31 47402.0 32 53392.0 33 61229.0 34 68796.0 35 75039.5 36 81915.0 37 87986.0 38 90734.5 39 92302.0 40 94145.5 41 97114.5 42 100655.0 43 103891.5 44 105969.0 45 106508.0 46 104961.0 47 109736.0 48 125129.5 49 121158.0 50 102290.5 51 91375.5 52 83545.5 53 78297.5 54 73426.0 55 66903.0 56 59130.0 57 52081.5 58 46721.0 59 41188.0 60 35162.5 61 28923.0 62 22445.5 63 16748.5 64 11857.5 65 8763.5 66 6239.5 67 4487.5 68 3086.0 69 1800.0 70 1250.0 71 966.5 72 832.5 73 699.5 74 539.5 75 434.0 76 300.5 77 161.5 78 98.5 79 65.5 80 36.0 81 27.5 82 20.0 83 12.0 84 8.0 85 5.0 86 3.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019019512982892468 2 0.0 3 0.0 4 3.458093269616812E-5 5 0.03534171321548382 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003596417000401484 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.70585389486774E-4 24-25 3.112283942655131E-4 26-27 0.0010892993799292959 28-29 0.0010028470481888755 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.70585389486774E-4 42-43 0.010443441674242772 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.458093269616812E-5 52-53 1.729046634808406E-5 54-55 2.4206652887317684E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.458093269616812E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.87875855834858E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010979446131033379 92-93 0.001694465702112238 94-95 0.0 96-97 0.0 98-99 0.0010547184472331275 100-101 0.016840914223033875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2891767.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.38093341191639 #Duplication Level Percentage of deduplicated Percentage of total 1 64.79359220736966 26.16425732444658 2 16.199491705184542 13.083011917078977 3 6.979954983626994 8.455712922360469 4 3.6909804520837324 5.961809434411129 5 2.185523415886457 4.412673776354754 6 1.4114610181509537 3.4197668032481614 7 0.9751632468930526 2.7564601496975576 8 0.6668612345636484 2.1542783286322433 9 0.5100099642255448 1.8535210564325024 >10 2.337019806971386 16.39157932754453 >50 0.1529507547587768 4.298217241082121 >100 0.09056496307004819 6.432417771901444 >500 0.003941431625571835 1.0659826128664471 >1k 0.002142082405167326 1.5800997748634786 >5k 8.568329620669303E-5 0.286866235293709 >10k+ 2.570498886200791E-4 1.6833453237859766 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20016 0.6921719488465011 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17118 0.5919564058930059 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11518 0.3983031827944644 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8291 0.2867105129839299 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3786 0.13092341118769252 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3309 0.11442830629162032 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2968 0.10263620824222698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.645233174042031E-5 0.0 22-23 0.0 0.0 0.0 2.247760625250928E-4 0.0 24-25 0.0 0.0 0.0 3.8039025965784935E-4 0.0 26-27 0.0 0.0 0.0 7.434900529676145E-4 0.0 28-29 0.0 0.0 0.0 0.0018327894328969106 0.0 30-31 0.0 0.0 0.0 0.0038211930629265776 0.0 32-33 0.0 0.0 0.0 0.006553086745923859 0.0 34-35 0.0 0.0 0.0 0.010287827477110018 0.0 36-37 0.0 0.0 0.0 0.014835220126656124 0.0 38-39 0.0 0.0 0.0 0.019192417646373305 0.0 40-41 0.0 0.0 0.0 0.02512304760376614 0.0 42-43 0.0 0.0 0.0 0.03468467549425663 0.0 44-45 0.0 0.0 0.0 0.04780813945245243 0.0 46-47 0.0 0.0 0.0 0.07644115172487964 0.0 48-49 0.0 0.0 0.0 0.1270157657930255 0.0 50-51 0.0 0.0 0.0 0.16541789155212022 0.0 52-53 0.0 0.0 0.0 0.2039756315083477 0.0 54-55 0.0 0.0 0.0 0.25489605490345524 0.0 56-57 0.0 0.0 0.0 0.29934984388437935 0.0 58-59 0.0 0.0 0.0 0.35314048469326886 0.0 60-61 0.0 0.0 0.0 0.43298785828872105 0.0 62-63 0.0 0.0 0.0 0.494680242218685 0.0 64-65 0.0 0.0 0.0 0.5663665156978415 0.0 66-67 0.0 0.0 0.0 0.6853249241726598 0.0 68-69 0.0 0.0 0.0 0.817199311009497 0.0 70-71 0.0 0.0 0.0 0.9355871340948285 0.0 72-73 0.0 0.0 0.0 1.0780951577357374 0.0 74-75 0.0 0.0 0.0 1.2161595315251885 0.0 76-77 0.0 0.0 0.0 1.3346510974086088 0.0 78-79 0.0 0.0 0.0 1.4946916539264747 0.0 80-81 0.0 0.0 0.0 1.7111164212054428 0.0 82-83 0.0 0.0 0.0 1.9185155650507113 0.0 84-85 0.0 0.0 0.0 2.1512971134949668 0.0 86-87 0.0 0.0 0.0 2.5234398207047803 0.0 88-89 0.0 0.0 0.0 2.9891412413240763 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14660 0.0 24.95883 1 GTATCAA 28125 0.0 23.85666 1 GGTATCA 21110 0.0 23.748255 1 TACATGG 15095 0.0 23.671303 2 GAGTACT 16230 0.0 22.507387 12-13 AGTACTT 16605 0.0 22.49972 12-13 ACATGGG 15220 0.0 22.477875 3 GTACTTT 16820 0.0 21.673277 14-15 AGAGTAC 26410 0.0 20.198942 10-11 ATCAACG 33460 0.0 19.952063 3 TCAACGC 33450 0.0 19.929619 4 CAACGCA 33600 0.0 19.826504 5 TATCAAC 33930 0.0 19.703695 2 AACGCAG 34070 0.0 19.559498 6 TACTTTT 19515 0.0 19.008799 14-15 CATGGGG 4940 0.0 18.948582 4 CATGGGA 10425 0.0 18.823978 4 GTGTAGC 1325 0.0 18.648973 1 ACTTTTT 19990 0.0 18.438309 16-17 ACGCAGA 37115 0.0 17.841467 7 >>END_MODULE