FastQCFastQC Report
Thu 26 May 2016
SRR937782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837249
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186670.6579260403299111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146790.5173673512617327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98860.34843610835707406No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57900.20407091517170328No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48100.16953041484903159No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36600.1289981950826311No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35650.1256498812758415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33860.11934095315568002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167450.027.8534221
GTATCAA234750.027.4427991
GTACATG137800.024.6689991
TACATGG141000.023.9657882
GAGTACT144900.023.915512-13
ATCAACG277250.023.0560133
TCAACGC277750.023.0181714
CAACGCA279000.022.931275
TATCAAC280200.022.9154512
ACATGGG139750.022.8534093
AACGCAG283000.022.6459356
GTACTTT152250.022.57215914-15
AGTACTT148850.021.39796412-13
ACGCAGA311550.020.4040377
CATGGGG44600.020.3564074
CGCAGAG312350.020.3369268
CATGGGA96850.019.9264074
AGAGTAC239600.019.82620410-11
GCAGAGT318700.019.3198179
ACTTTTT182950.019.21163616-17
Produced by FastQC (version 0.11.5)