##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937782_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2837249 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.785860176530154 33.0 31.0 34.0 30.0 34.0 2 31.90489590444829 33.0 31.0 34.0 30.0 34.0 3 31.938856265347173 33.0 31.0 34.0 30.0 34.0 4 35.54169937146863 37.0 35.0 37.0 33.0 37.0 5 35.53337264371227 37.0 35.0 37.0 33.0 37.0 6 35.62541497062824 37.0 35.0 37.0 33.0 37.0 7 35.5634764520139 37.0 35.0 37.0 33.0 37.0 8 35.55939785334315 37.0 35.0 37.0 33.0 37.0 9 37.17941076021174 39.0 37.0 39.0 34.0 39.0 10-11 37.07762713106957 39.0 37.0 39.0 33.0 39.0 12-13 36.96421304580599 39.0 37.0 39.0 33.0 39.0 14-15 38.09455320981698 40.0 38.0 41.0 33.0 41.0 16-17 38.05322937817583 40.0 38.0 41.0 33.0 41.0 18-19 38.04607015457579 40.0 38.0 41.0 33.0 41.0 20-21 37.89877360076609 40.0 38.0 41.0 33.0 41.0 22-23 37.77335774900264 40.0 37.5 41.0 33.0 41.0 24-25 37.63484699439492 40.0 37.0 41.0 32.0 41.0 26-27 37.719802527025294 40.0 37.0 41.0 33.0 41.0 28-29 37.66657191526017 40.0 37.0 41.0 33.0 41.0 30-31 37.45195152064552 40.0 37.0 41.0 32.0 41.0 32-33 37.19604518320387 39.0 36.0 41.0 32.0 41.0 34-35 36.929741978938054 39.0 36.0 41.0 31.0 41.0 36-37 36.93429709553162 39.0 36.0 41.0 31.0 41.0 38-39 36.883924886395235 39.0 36.0 41.0 31.0 41.0 40-41 36.69836750316944 39.0 36.0 41.0 30.5 41.0 42-43 36.44230731951971 39.0 35.0 41.0 30.0 41.0 44-45 36.1643429956271 39.0 35.0 41.0 30.0 41.0 46-47 35.7437464952847 39.0 35.0 41.0 28.0 41.0 48-49 35.36963040607293 39.0 35.0 40.5 26.5 41.0 50-51 34.46191381158298 38.0 34.0 39.5 25.0 40.5 52-53 34.563395387574374 38.0 34.5 39.5 24.5 40.5 54-55 34.88877007270071 39.0 35.0 40.0 25.0 41.0 56-57 34.648741086876754 38.5 34.5 40.0 23.5 41.0 58-59 34.44599028848015 38.0 34.0 40.0 23.0 41.0 60-61 34.161834403677645 38.0 34.0 40.0 22.5 41.0 62-63 33.83174159194346 38.0 34.0 40.0 22.0 41.0 64-65 33.48616952548049 37.0 33.0 40.0 20.5 41.0 66-67 33.0883567145499 36.5 33.0 39.5 19.5 41.0 68-69 32.64084294328767 36.0 32.0 39.0 17.5 40.5 70-71 32.207097085944866 35.5 32.0 39.0 13.5 40.0 72-73 31.750962816446496 35.0 31.0 38.0 10.0 40.0 74-75 31.27732338613918 35.0 31.0 37.5 7.5 39.5 76-77 30.826795251315623 35.0 30.5 37.0 4.5 39.0 78-79 30.30008857171154 34.5 30.0 36.0 2.0 39.0 80-81 29.778148128697907 34.0 29.5 36.0 2.0 37.5 82-83 29.275025914186593 34.0 29.0 35.0 2.0 37.0 84-85 28.8166592005143 34.0 29.0 35.0 2.0 37.0 86-87 28.318912087025144 34.0 28.0 35.0 2.0 36.0 88-89 27.849524486571323 34.0 27.0 35.0 2.0 36.0 90-91 27.35603343238468 33.0 26.0 35.0 2.0 35.5 92-93 26.831941962090745 33.0 25.0 35.0 2.0 35.0 94-95 26.240459156034596 32.5 24.5 35.0 2.0 35.0 96-97 25.516607460254633 32.0 21.5 34.0 2.0 35.0 98-99 24.640588647665396 32.0 10.0 34.0 2.0 35.0 100-101 22.163777130593754 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19149195753965742 1101 2 0.23325143139863513 1101 3 0.2284661628475213 1101 4 0.11717845225782497 1101 5 0.148672487017798 1101 6 0.13282746697760217 1101 7 0.12101676375256432 1101 8 0.15686026101224826 1101 9 0.24393014359002052 1101 10-11 0.1927183307584457 1101 12-13 0.19606132589959913 1101 14-15 0.35553661070494513 1101 16-17 0.46655266455467626 1101 18-19 0.37132217046271876 1101 20-21 0.3941651436912821 1101 22-23 0.4269340295340953 1101 24-25 0.3027355786177637 1101 26-27 0.3679352551755031 1101 28-29 0.34440961334127707 1101 30-31 0.2962647251452779 1101 32-33 0.30105204597762025 1101 34-35 0.3338253071743651 1101 36-37 0.3606321319805943 1101 38-39 0.4810071945835759 1101 40-41 0.5569235745387004 1101 42-43 0.5622295230705561 1101 44-45 0.5424982819721578 1101 46-47 0.663115542046441 1101 48-49 0.7434601910038268 1101 50-51 0.7210932334184719 1101 52-53 0.7209544573251421 1101 54-55 0.8776526901429378 1101 56-57 0.9136754866099182 1101 58-59 0.899660686851746 1101 60-61 0.9397613056387044 1101 62-63 0.934523126406198 1101 64-65 0.9546189841721144 1101 66-67 1.1078278253355194 1101 68-69 1.060315676065919 1101 70-71 1.0074169189281434 1101 72-73 0.8877104971214465 1101 74-75 0.8764861864731373 1101 76-77 0.9230160648829902 1101 78-79 0.8825705183830301 1101 80-81 1.0423963259684612 1101 82-83 1.073443829736167 1101 84-85 1.170703750453729 1101 86-87 1.3462187108066281 1101 88-89 1.2959863146439794 1101 90-91 1.418689492392808 1101 92-93 1.7919097232393177 1101 94-95 1.7814313842690659 1101 96-97 1.6420663934468323 1101 98-99 1.8498626910126035 1101 100-101 2.1618689306889216 1102 1 0.20484599480673538 1102 2 0.2624439779824854 1102 3 0.29492579017670906 1102 4 0.20382441499074133 1102 5 0.15798925720413592 1102 6 0.23406970300244723 1102 7 0.11045775754138987 1102 8 0.15220187591908285 1102 9 0.23306057837262983 1102 10-11 0.24753199535471282 1102 12-13 0.3094153631666785 1102 14-15 0.40957387778568943 1102 16-17 0.40319862728759404 1102 18-19 0.23653956176707425 1102 20-21 0.25565582692109245 1102 22-23 0.3902880668011761 1102 24-25 0.44854303203391765 1102 26-27 0.5013203483432065 1102 28-29 0.43136613508040256 1102 30-31 0.3198672096173283 1102 32-33 0.35043092796519915 1102 34-35 0.3456265494103974 1102 36-37 0.40162592080046977 1102 38-39 0.5428084368195982 1102 40-41 0.507389413048017 1102 42-43 0.4423537466730352 1102 44-45 0.5508833751398541 1102 46-47 0.6270906973259471 1102 48-49 0.674360812121833 1102 50-51 0.6113106247228188 1102 52-53 0.6406749542195485 1102 54-55 0.5256340566025699 1102 56-57 0.6101040580384876 1102 58-59 0.6265240408890236 1102 60-61 0.7254755913529962 1102 62-63 0.757193933859611 1102 64-65 0.7234078040478877 1102 66-67 0.640281241484594 1102 68-69 0.6811231294820601 1102 70-71 0.8314852419095047 1102 72-73 0.6429900002270443 1102 74-75 0.5072315280880453 1102 76-77 0.5733266238891979 1102 78-79 0.5549307668302355 1102 80-81 0.6630484998815049 1102 82-83 0.7127295440218795 1102 84-85 0.8750515765406846 1102 86-87 0.8646969716761923 1102 88-89 0.810272028929699 1102 90-91 1.065739181833802 1102 92-93 1.2075929530529805 1102 94-95 1.2652823159460844 1102 96-97 1.3120974493474513 1102 98-99 1.5065397096461481 1102 100-101 1.8932353903162529 1103 1 0.16241118822436462 1103 2 0.25907058197130794 1103 3 0.20638181146533796 1103 4 0.1659951515220328 1103 5 0.09520529399573974 1103 6 0.21559338999413313 1103 7 0.14508853294999113 1103 8 0.1471365376604865 1103 9 0.16444561759956144 1103 10-11 0.2591133789954796 1103 12-13 0.20424509012647718 1103 14-15 0.4075499001131746 1103 16-17 0.28781681651582147 1103 18-19 0.25442132758819724 1103 20-21 0.3307752957443242 1103 22-23 0.2530152423624088 1103 24-25 0.2770657141289732 1103 26-27 0.09900449115439613 1103 28-29 0.22642630900942606 1103 30-31 0.26213430035170404 1103 32-33 0.2920016832758705 1103 34-35 0.41845448433784327 1103 36-37 0.47011107410214237 1103 38-39 0.4855619434692997 1103 40-41 0.5460571059212427 1103 42-43 0.5104502267379232 1103 44-45 0.38087429510972726 1103 46-47 0.5912955004521123 1103 48-49 0.5298249125395742 1103 50-51 0.3814437185857571 1103 52-53 0.47348862480924225 1103 54-55 0.4736004894592938 1103 56-57 0.6742255526374237 1103 58-59 0.7316742359748147 1103 60-61 0.683296903552197 1103 62-63 0.5165347276680095 1103 64-65 0.7030088520971347 1103 66-67 0.8035977699363528 1103 68-69 0.7232775938801552 1103 70-71 0.8062246280635108 1103 72-73 0.7477705012369285 1103 74-75 0.8082900158684652 1103 76-77 0.7333084138837869 1103 78-79 0.6942499943184508 1103 80-81 0.8614111976393666 1103 82-83 0.7878345786938112 1103 84-85 0.9276225036627075 1103 86-87 0.9680493907200862 1103 88-89 0.9697576137575545 1103 90-91 1.102319416735881 1103 92-93 1.2756211580838546 1103 94-95 1.2622493461469197 1103 96-97 1.379063520601214 1103 98-99 1.450683240049763 1103 100-101 1.9159838624937997 1104 1 0.16113035446974777 1104 2 0.201405512252947 1104 3 0.32928320972728997 1104 4 0.1858271626954675 1104 5 0.2966599962854275 1104 6 0.22506188935066973 1104 7 0.2669448832841326 1104 8 0.309352318094426 1104 9 0.46187333917489326 1104 10-11 0.41946256773622537 1104 12-13 0.4191288519184013 1104 14-15 0.6106214669204988 1104 16-17 0.5289451119989508 1104 18-19 0.5705749913469873 1104 20-21 0.5983004286214211 1104 22-23 0.4536020956926734 1104 24-25 0.61187356638343 1104 26-27 0.5245745623094891 1104 28-29 0.5593100891685197 1104 30-31 0.5124673160214499 1104 32-33 0.4805471392094276 1104 34-35 0.5668356890540451 1104 36-37 0.574504889321517 1104 38-39 0.6021617967753272 1104 40-41 0.7294433223310293 1104 42-43 0.6532483638639235 1104 44-45 0.6463605057327442 1104 46-47 0.7063900753532835 1104 48-49 0.7770057449996415 1104 50-51 0.7571409934835955 1104 52-53 0.7572625656180421 1104 54-55 0.825845262366343 1104 56-57 0.9780857129430132 1104 58-59 0.9237963670582374 1104 60-61 0.9140362940474986 1104 62-63 0.8833988857106831 1104 64-65 0.8974500601570483 1104 66-67 0.9209429874949748 1104 68-69 1.0108265974443853 1104 70-71 0.9871268501927943 1104 72-73 0.8992964205831271 1104 74-75 0.8292675965199976 1104 76-77 0.8732968713321902 1104 78-79 0.9046548212117109 1104 80-81 1.0317147306758834 1104 82-83 1.152841439275786 1104 84-85 1.053853173457103 1104 86-87 1.0785315367760724 1104 88-89 1.0886065053594756 1104 90-91 1.3505327941681315 1104 92-93 1.3251777291449578 1104 94-95 1.324140138251206 1104 96-97 1.5300718397138198 1104 98-99 1.8379891773050616 1104 100-101 1.4700069761435621 1105 1 0.08396780118794567 1105 2 0.1548847158799873 1105 3 0.10203068658302428 1105 4 0.09962124151546448 1105 5 0.23295304118111204 1105 6 0.205649059975066 1105 7 0.2596808841948999 1105 8 0.11337227204306544 1105 9 0.09017816360452713 1105 10-11 0.23526644784246997 1105 12-13 0.21160381548948237 1105 14-15 0.23777606153257835 1105 16-17 0.26917132093493024 1105 18-19 0.2929500621931993 1105 20-21 0.4287783036952604 1105 22-23 0.35467354697806286 1105 24-25 0.42702722492943934 1105 26-27 0.4104453057827655 1105 28-29 0.26352252697055434 1105 30-31 0.20450580014258435 1105 32-33 0.21971043530039935 1105 34-35 0.2825479653987344 1105 36-37 0.2968442954887962 1105 38-39 0.3012379937469589 1105 40-41 0.3892574513767286 1105 42-43 0.3090452094197005 1105 44-45 0.291738697150528 1105 46-47 0.37686970028033073 1105 48-49 0.26113355737039257 1105 50-51 0.17464911817855722 1105 52-53 0.03387236908282176 1105 54-55 0.10794953464349533 1105 56-57 0.3291932329899083 1105 58-59 0.4185134266864168 1105 60-61 0.2443607934331169 1105 62-63 0.45846635871533437 1105 64-65 0.48872739326831294 1105 66-67 0.3308938897059335 1105 68-69 0.2518101976654705 1105 70-71 0.3881022001019403 1105 72-73 0.3450755673223824 1105 74-75 0.44958370855697893 1105 76-77 0.35799075067594543 1105 78-79 0.21001211277625842 1105 80-81 0.4123125479975336 1105 82-83 0.5258913455813392 1105 84-85 0.4779446816443418 1105 86-87 0.4564030265722856 1105 88-89 0.3787135450751684 1105 90-91 0.3463696895771555 1105 92-93 0.43879784163451774 1105 94-95 0.6207239187985287 1105 96-97 0.5657542631410521 1105 98-99 0.8411563062070542 1105 100-101 0.9856511206533298 1106 1 0.17127175112839055 1106 2 0.2350296592944403 1106 3 0.27988885731981483 1106 4 0.3073364571433217 1106 5 0.21897624939137472 1106 6 0.23339385303082594 1106 7 0.19324971553236736 1106 8 0.27494625748158796 1106 9 0.22708683176814048 1106 10-11 -0.04528050955209295 1106 12-13 -0.17418832137086326 1106 14-15 0.07234627583058995 1106 16-17 0.03309674509140592 1106 18-19 4.2992737206759557E-4 1106 20-21 0.176298916226159 1106 22-23 0.05892693456740972 1106 24-25 0.16594039278705708 1106 26-27 0.0857190482348571 1106 28-29 0.3049399637828216 1106 30-31 0.37506091364753047 1106 32-33 0.4965659319691582 1106 34-35 0.2152589480385103 1106 36-37 0.3230242662802567 1106 38-39 0.2937619281129571 1106 40-41 0.17753980256460267 1106 42-43 0.22975737692724607 1106 44-45 0.12332387095757724 1106 46-47 0.25066099312526546 1106 48-49 0.5134941629514529 1106 50-51 0.3865312909722718 1106 52-53 0.3830647444660471 1106 54-55 0.4523945078271936 1106 56-57 0.5334006940418021 1106 58-59 0.34484135839494456 1106 60-61 0.17656787526830442 1106 62-63 0.4753900111034568 1106 64-65 0.3787519648880675 1106 66-67 0.5893725002972161 1106 68-69 0.5776244899301517 1106 70-71 0.31794766191448787 1106 72-73 0.3525411196526278 1106 74-75 0.3614471787366398 1106 76-77 0.5898330762039805 1106 78-79 0.5135612565691119 1106 80-81 0.42461735560222635 1106 82-83 0.39788338793261246 1106 84-85 0.4658209979745287 1106 86-87 0.20788531017990408 1106 88-89 0.6142460402215271 1106 90-91 0.9650305824111776 1106 92-93 0.9174383128371026 1106 94-95 0.7938020407609692 1106 96-97 0.8466566439801326 1106 98-99 1.0016263947512023 1106 100-101 0.8669533390342004 1107 1 0.4615744388007563 1107 2 0.5240254708732408 1107 3 0.42294132314413346 1107 4 0.3834780338309898 1107 5 0.4668304055198931 1107 6 0.43644680893367394 1107 7 0.5434407294756838 1107 8 0.5329758150515218 1107 9 0.6327971801848093 1107 10-11 0.5810485367609104 1107 12-13 0.6366361861731349 1107 14-15 0.8538328007128726 1107 16-17 0.7630306545003833 1107 18-19 0.780970571510295 1107 20-21 0.8203800364756759 1107 22-23 0.8819732131502249 1107 24-25 0.8017118376611094 1107 26-27 0.772645065629149 1107 28-29 0.7876932104072623 1107 30-31 0.7643677063110559 1107 32-33 0.8722859536026206 1107 34-35 0.8903987225207004 1107 36-37 0.8932290374281706 1107 38-39 0.8842497517033507 1107 40-41 1.0176940518663926 1107 42-43 1.052138111356605 1107 44-45 0.9833819452639787 1107 46-47 1.1069269389097514 1107 48-49 1.079015183528334 1107 50-51 1.0238358356922248 1107 52-53 0.9661453081263716 1107 54-55 1.0861933136691704 1107 56-57 1.0547698684519986 1107 58-59 1.0615507319053847 1107 60-61 1.2096303089399711 1107 62-63 1.2091603894732614 1107 64-65 1.2923862706188984 1107 66-67 1.2628772940237525 1107 68-69 1.4194646239782998 1107 70-71 1.2559684797505497 1107 72-73 1.3653748325051112 1107 74-75 1.2611363435086247 1107 76-77 1.211890125429452 1107 78-79 1.4201622060379329 1107 80-81 1.5358597863684231 1107 82-83 1.625086932717334 1107 84-85 1.6279045551979046 1107 86-87 1.6821665429141142 1107 88-89 1.6381874705857449 1107 90-91 1.867735990952898 1107 92-93 1.9471731715982195 1107 94-95 2.0291995236242677 1107 96-97 2.0879072106334355 1107 98-99 2.1890580973482088 1107 100-101 2.145012450090082 1108 1 0.15463199714380238 1108 2 0.2008060037181849 1108 3 0.22621049919819924 1108 4 0.275695442622677 1108 5 0.2343516701181656 1108 6 0.14600039341605253 1108 7 0.20270136268479177 1108 8 0.2144066232852211 1108 9 0.2019555807400195 1108 10-11 0.27162300092215474 1108 12-13 0.2503248940511469 1108 14-15 0.37427720821907684 1108 16-17 0.27487291701361016 1108 18-19 0.31389452732918954 1108 20-21 0.29040584033239014 1108 22-23 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-0.8643865118104479 2316 86-87 -0.8009785103036329 2316 88-89 -0.7465047430644667 2316 90-91 -1.0362797745329821 2316 92-93 -0.815186646900532 2316 94-95 -0.7305884114463836 2316 96-97 -0.775700680841986 2316 98-99 -0.4295823058236934 2316 100-101 0.034139756220614714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9250.0 3 1535.0 4 1499.0 5 1722.0 6 2070.0 7 2348.0 8 3022.0 9 3980.0 10 5164.0 11 7028.0 12 10173.0 13 13812.0 14 16107.0 15 20626.0 16 26838.0 17 36279.0 18 30914.0 19 19422.0 20 18012.0 21 18634.0 22 19815.0 23 22395.0 24 25246.0 25 28369.0 26 33105.0 27 38894.0 28 46201.0 29 56610.0 30 69334.0 31 86102.0 32 109587.0 33 143132.0 34 193336.0 35 271972.0 36 390981.0 37 545344.0 38 450503.0 39 57878.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81328582588379 19.3612651686267 14.99715450171967 21.828294503769843 2 14.746370525632905 20.83164758189925 41.356450123723846 23.065531768743995 3 18.612083348349717 25.668900397916506 31.30297691858152 24.41603933515226 4 11.832199846151509 17.19308127009456 39.05098024527421 31.92373863847972 5 12.536979268994234 38.2503098979351 35.03313116350596 14.179579669564701 6 29.869323123941204 37.423917791838726 18.196833567532043 14.509925516688027 7 26.67837385650088 34.41871436551998 21.636772488080346 17.26613928989879 8 25.56923495232025 34.69570944043165 20.620957903044406 19.114097704203694 9 26.185617762935777 16.87188604056217 21.506538343638447 35.43595785286361 10-11 23.958067730983107 27.222495721521366 28.13158855691489 20.68784799058064 12-13 25.405433943127832 25.10935936793163 28.63073631246603 20.854470376474502 14-15 22.309157326503932 26.053721503645544 28.04203020258659 23.595090967263932 16-17 20.63536761679853 29.45040960676656 28.507380972516156 21.40684180391876 18-19 20.442306755190636 27.559230008128743 31.728336121777197 20.270127114903428 20-21 21.617368409252244 26.400611962540065 31.634565291039703 20.347454337167985 22-23 21.07623896348965 26.525654987139667 31.647595096547835 20.750510952822847 24-25 20.94077492302659 26.57816453444572 31.925439563669926 20.555620978857757 26-27 20.81079439580699 26.769795805082786 31.861585881959115 20.557823917151108 28-29 20.386881403019824 27.10122697490801 31.957426589241418 20.554465032830745 30-31 20.643898663067517 26.74853740455212 31.801470523320834 20.806093409059528 32-33 20.538585819347205 26.882693105670675 31.699047383615703 20.879673691366417 34-35 21.103595713845817 26.769641812886146 31.45082519485729 20.675937278410743 36-37 20.993245167357816 26.558403853225876 31.603086803686498 20.84526417572981 38-39 20.75144387243259 26.987179249544692 31.566775249474784 20.69460162854794 40-41 20.874446661397993 26.975332974046818 31.46124815995111 20.68897220460408 42-43 20.745723703781778 27.030559891274812 30.98553032040421 21.238186084539198 44-45 20.903711824893033 27.118151829165487 30.784745228580583 21.193391117360896 46-47 21.20692586489892 27.396918059680175 30.233720487013166 21.162435588407742 48-49 21.249423933637154 27.492976846105083 29.993767006097034 21.26383221416073 50-51 21.074625969671477 27.68568651460323 30.034050509874827 21.205637005850473 52-53 21.315505720995134 28.019720189177626 29.443126099936674 21.22164798989056 54-55 21.118233712544328 27.652371617948674 29.618569774805625 21.610824894701373 56-57 21.349880713758033 28.002884405762103 29.34863445276168 21.29860042771819 58-59 21.480676380096536 27.556033281298575 29.563252243723216 21.400038094881676 60-61 21.344006354389602 27.650014341504313 29.976797652406063 21.029181651700018 62-63 21.12342580741166 27.68813745058073 30.005134997114762 21.183301744892848 64-65 21.077512517464434 27.778753540326402 29.87289687621633 21.270837065992833 66-67 21.015177334859857 27.82759103982625 29.50087182034464 21.656359804969252 68-69 20.96034047841383 27.841930319136733 29.697226993258568 21.50050220919087 70-71 21.219922081177504 27.803974318916406 29.47315648902799 21.502947110878097 72-73 21.283653599193507 27.5665119691271 29.591548716485853 21.55828571519354 74-75 20.830682985570633 28.190802441444745 29.262346207005884 21.71616836597873 76-77 21.046014535031 27.908449323073715 29.256970726400255 21.788565415495032 78-79 21.10781870773609 27.926500846050573 29.20388274100966 21.761797705203676 80-81 21.008593831781344 28.174786495805648 29.123562612215586 21.693057060197425 82-83 21.15340694283585 27.98730760283627 29.23970931318232 21.619576141145554 84-85 21.079402541670632 27.846230391783966 29.061045384403723 22.01332168214168 86-87 21.133099525029078 27.923542213459807 29.152624342303827 21.790733919207288 88-89 21.195451105098762 27.66794295736064 29.05097561044492 22.085630327095675 90-91 21.109246842241372 27.536626116612222 29.324582547442507 22.029544493703902 92-93 21.278007865158028 27.523975599264215 29.400683713432034 21.79733282214572 94-95 20.951819129865022 27.48453903773823 29.431165797859084 22.13247603453766 96-97 20.833509119181393 27.535654224323693 29.499136545798375 22.131700110696542 98-99 20.908180153143412 27.67512412560408 29.084890879802284 22.33180484145023 100-101 21.043525473702346 27.028428142053162 29.69923245993379 22.228813924310707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3829.0 1 2315.5 2 883.0 3 942.5 4 778.5 5 764.0 6 1316.0 7 2861.5 8 4945.0 9 6010.5 10 5490.0 11 4809.5 12 4904.5 13 5249.5 14 5580.5 15 5830.5 16 6184.0 17 6569.5 18 6931.5 19 7362.5 20 8096.5 21 9273.0 22 10825.0 23 12809.0 24 15119.5 25 18283.5 26 22785.5 27 27405.5 28 30902.5 29 35567.0 30 41441.5 31 46395.5 32 52423.0 33 60522.0 34 67951.5 35 73960.5 36 80899.5 37 86953.0 38 89427.5 39 90226.5 40 92441.0 41 96233.5 42 99537.5 43 102181.0 44 103621.5 45 104458.0 46 104212.5 47 108262.5 48 122006.0 49 118924.0 50 100107.5 51 88879.0 52 81839.5 53 76787.0 54 71975.5 55 65557.5 56 58420.5 57 51617.5 58 45647.0 59 39750.0 60 33770.0 61 27873.5 62 22025.0 63 16415.5 64 11720.5 65 8711.5 66 6140.5 67 4411.5 68 3054.0 69 1915.5 70 1399.0 71 1064.0 72 864.0 73 726.5 74 520.0 75 395.5 76 294.5 77 178.0 78 107.5 79 77.0 80 50.0 81 40.5 82 30.5 83 20.0 84 13.5 85 11.0 86 7.5 87 8.0 88 7.0 89 4.0 90 6.0 91 6.0 92 3.5 93 3.0 94 1.5 95 1.5 96 1.0 97 0.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28968201240003966 2 0.2522161431724886 3 0.24696457730710278 4 0.2534849778782194 5 0.2567628008680239 6 0.19765625082606425 7 0.1893030890133365 8 0.1879990088991132 9 0.19896033094028756 10-11 0.19860787685536235 12-13 0.19490710896364752 14-15 0.19635217071184097 16-17 0.18339948309083906 18-19 0.18761130940569545 20-21 0.17797169018299064 22-23 0.1862719838829796 24-25 0.1855494530088829 26-27 0.1867301741933824 28-29 0.19485424085090877 30-31 0.19272189363711115 32-33 0.1964579069373185 34-35 0.19148830433987288 36-37 0.18075607745389988 38-39 0.17088736307599367 40-41 0.16859641152397975 42-43 0.18167245807470547 44-45 0.1815667218492279 46-47 0.1822011392020933 48-49 0.19540054468254286 50-51 0.19125920918467149 52-53 0.20569220396235932 54-55 0.18272982032948112 56-57 0.1683496936645321 58-59 0.17085211766750116 60-61 0.16077193083863983 62-63 0.16655217783141346 64-65 0.16588251507005553 66-67 0.16459605766007848 68-69 0.16790912605837557 70-71 0.16967139648300167 72-73 0.16679889569086112 74-75 0.17881757998681116 76-77 0.17095785389297874 78-79 0.1752225483205739 80-81 0.16954803755327782 82-83 0.15409292592930687 84-85 0.15326465882973261 86-87 0.15663059534076845 88-89 0.16075430813439356 90-91 0.16711610436729382 92-93 0.16316861861613133 94-95 0.1632214867288701 96-97 0.16672840487387605 98-99 0.1739889590233356 100-101 0.1608247989513786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2837249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.6302350442026 #Duplication Level Percentage of deduplicated Percentage of total 1 66.94935127200222 28.54066580782339 2 15.39528791779098 13.126094850172038 3 6.610477715650751 8.454186563179649 4 3.483876580692237 5.940739099996117 5 2.0015798025777523 4.266390872180911 6 1.2979618293200228 3.3199450717389443 7 0.8717510075675622 2.6014065246837927 8 0.632258878156019 2.156267566765995 9 0.4596985862279044 1.763735290234488 >10 2.068651453257044 15.415257394960255 >50 0.14439538653185255 4.2647150803327945 >100 0.07891744207896094 5.792743879000254 >500 0.0031442981368887683 0.890392742212478 >1k 0.002316851258713078 1.7382447329867534 >5k 1.6548937562236272E-4 0.5529592198202108 >10k+ 1.6548937562236272E-4 1.1762553039120152 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18667 0.6579260403299111 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14679 0.5173673512617327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9886 0.34843610835707406 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5790 0.20407091517170328 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4810 0.16953041484903159 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3660 0.1289981950826311 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3565 0.1256498812758415 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3386 0.11934095315568002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7622704246261078E-5 0.0 12-13 0.0 0.0 0.0 3.5245408492522156E-5 0.0 14-15 0.0 0.0 0.0 3.5245408492522156E-5 0.0 16-17 0.0 0.0 0.0 3.5245408492522156E-5 0.0 18-19 0.0 0.0 0.0 3.5245408492522156E-5 0.0 20-21 0.0 0.0 0.0 3.5245408492522156E-5 0.0 22-23 0.0 0.0 0.0 1.5860433821634972E-4 0.0 24-25 0.0 0.0 0.0 4.053221976640048E-4 0.0 26-27 0.0 0.0 0.0 8.282670995742706E-4 0.0 28-29 0.0 0.0 0.0 0.002273328847767679 0.0 30-31 0.0 0.0 0.0 0.004828620963475536 0.0 32-33 0.0 0.0 0.0 0.0091461835038095 0.0 34-35 0.0 0.0 0.0 0.013604727678113554 0.0 36-37 0.0 0.0 0.0 0.01852146216282039 0.0 38-39 0.0 0.0 0.0 0.023596800985743585 0.0 40-41 0.0 0.0 0.0 0.030187692373845228 0.0 42-43 0.0 0.0 0.0 0.039985915934766386 0.0 44-45 0.0 0.0 0.0 0.054595137754916825 0.0 46-47 0.0 0.0 0.0 0.0862983826939405 0.0 48-49 0.0 0.0 0.0 0.13950132681340272 0.0 50-51 0.0 0.0 0.0 0.1834171057950853 0.0 52-53 0.0 0.0 0.0 0.22742099829799922 0.0 54-55 0.0 0.0 0.0 0.2886598955537565 0.0 56-57 0.0 0.0 0.0 0.33971286975517484 0.0 58-59 0.0 0.0 0.0 0.4000882545028653 0.0 60-61 0.0 0.0 0.0 0.48858947522758844 0.0 62-63 0.0 0.0 0.0 0.5522603056693296 0.0 64-65 0.0 0.0 0.0 0.6263109089121187 0.0 66-67 0.0 0.0 0.0 0.753088643259721 0.0 68-69 0.0 0.0 0.0 0.8949161670336301 0.0 70-71 0.0 0.0 0.0 1.020654161830703 0.0 72-73 1.7622704246261078E-5 1.7622704246261078E-5 0.0 1.1672221930468563 0.0 74-75 3.5245408492522156E-5 3.5245408492522156E-5 0.0 1.3082743178339298 0.0 76-77 3.5245408492522156E-5 3.5245408492522156E-5 0.0 1.430382035556273 0.0 78-79 3.5245408492522156E-5 3.5245408492522156E-5 0.0 1.5909248712397115 0.0 80-81 3.5245408492522156E-5 3.5245408492522156E-5 0.0 1.8120369414175492 0.0 82-83 3.5245408492522156E-5 3.5245408492522156E-5 0.0 2.0184692989582516 0.0 84-85 3.5245408492522156E-5 3.5245408492522156E-5 0.0 2.252393075123121 0.0 86-87 3.5245408492522156E-5 3.5245408492522156E-5 0.0 2.6268579176519227 0.0 88-89 3.5245408492522156E-5 3.5245408492522156E-5 0.0 3.103552067513285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16745 0.0 27.853422 1 GTATCAA 23475 0.0 27.442799 1 GTACATG 13780 0.0 24.668999 1 TACATGG 14100 0.0 23.965788 2 GAGTACT 14490 0.0 23.9155 12-13 ATCAACG 27725 0.0 23.056013 3 TCAACGC 27775 0.0 23.018171 4 CAACGCA 27900 0.0 22.93127 5 TATCAAC 28020 0.0 22.915451 2 ACATGGG 13975 0.0 22.853409 3 AACGCAG 28300 0.0 22.645935 6 GTACTTT 15225 0.0 22.572159 14-15 AGTACTT 14885 0.0 21.397964 12-13 ACGCAGA 31155 0.0 20.404037 7 CATGGGG 4460 0.0 20.356407 4 CGCAGAG 31235 0.0 20.336926 8 CATGGGA 9685 0.0 19.926407 4 AGAGTAC 23960 0.0 19.826204 10-11 GCAGAGT 31870 0.0 19.319817 9 ACTTTTT 18295 0.0 19.211636 16-17 >>END_MODULE