FastQCFastQC Report
Thu 26 May 2016
SRR937782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837249
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198640.7001147942954602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166920.5883163585571799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112160.3953125016521285No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85120.30000891708834865No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37850.13340387114419638No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32850.1157811668979353No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.10291659279816472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA282000.023.977531
GTACATG141150.023.6659031
GGTATCA212650.023.3283371
TACATGG145050.022.7299522
GAGTACT166750.022.3467812-13
AGTACTT169200.021.9951312-13
ACATGGG143400.021.8651073
GTACTTT175500.021.21834614-15
CGTATAG2509.464202E-920.9074631
ATCAACG331950.020.2936573
TCAACGC332600.020.2539984
CAACGCA335350.020.0879085
TATCAAC339300.019.938062
CATGGGA97450.019.8900154
AACGCAG341100.019.7733026
AGAGTAC268250.019.7434510-11
GTAGCGG1707.962073E-519.5578756
TACTTTT200300.018.46078914-15
ACTTTTT207700.018.0433416-17
ATGGGAG41100.017.9162315
Produced by FastQC (version 0.11.5)