##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937782_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2837249 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21533570018 33.0 31.0 34.0 31.0 34.0 2 32.41899776861319 34.0 31.0 34.0 31.0 34.0 3 32.50276764570187 34.0 31.0 34.0 31.0 34.0 4 36.129229757416425 37.0 35.0 37.0 35.0 37.0 5 36.0525216503733 37.0 35.0 37.0 35.0 37.0 6 36.089983290151835 37.0 35.0 37.0 35.0 37.0 7 36.03711588232122 37.0 35.0 37.0 35.0 37.0 8 36.023967230229 37.0 35.0 37.0 35.0 37.0 9 37.68975969328036 39.0 37.0 39.0 35.0 39.0 10-11 37.62950379046745 39.0 37.0 39.0 35.0 39.0 12-13 37.540460319133075 39.0 37.0 39.0 35.0 39.0 14-15 38.73616573659908 40.0 38.0 41.0 34.5 41.0 16-17 38.71324881954315 40.0 38.0 41.0 34.5 41.0 18-19 38.73756057364017 40.0 38.0 41.0 35.0 41.0 20-21 38.65015918588746 40.0 38.0 41.0 34.0 41.0 22-23 38.49639192753262 40.0 38.0 41.0 34.0 41.0 24-25 38.56088820544126 40.0 38.0 41.0 34.0 41.0 26-27 38.431193561086815 40.0 38.0 41.0 34.0 41.0 28-29 38.29544340309927 40.0 38.0 41.0 34.0 41.0 30-31 38.30317747931183 40.0 38.0 41.0 34.0 41.0 32-33 38.19508210241682 40.0 37.0 41.0 34.0 41.0 34-35 38.049762287342425 40.0 37.0 41.0 34.0 41.0 36-37 37.87407714303538 40.0 37.0 41.0 33.0 41.0 38-39 37.72953959980248 40.0 37.0 41.0 33.0 41.0 40-41 37.551463406983316 40.0 37.0 41.0 33.0 41.0 42-43 37.31108602029642 40.0 37.0 41.0 32.5 41.0 44-45 36.95340363147542 40.0 36.0 41.0 31.5 41.0 46-47 36.52896097593126 40.0 36.0 41.0 30.5 41.0 48-49 36.14668627956165 39.0 36.0 41.0 30.0 41.0 50-51 35.8792661483007 39.0 35.5 41.0 29.0 41.0 52-53 35.645067810403674 39.0 35.0 40.5 28.0 41.0 54-55 35.43473977786229 39.0 35.0 40.0 27.5 41.0 56-57 35.18566153340789 38.5 35.0 40.0 27.0 41.0 58-59 34.93786393087106 38.0 34.5 40.0 26.0 41.0 60-61 34.59926922170031 38.0 34.0 40.0 26.0 41.0 62-63 34.27478377823025 38.0 34.0 40.0 25.5 41.0 64-65 33.901636232843856 37.0 33.5 40.0 24.5 41.0 66-67 33.4791096939324 36.5 33.0 39.0 23.0 41.0 68-69 33.055948737668075 36.0 33.0 39.0 22.0 40.0 70-71 32.64216253138163 35.5 32.0 39.0 21.5 40.0 72-73 32.150133280512215 35.0 31.5 38.0 19.5 40.0 74-75 31.652243599345706 35.0 31.0 37.0 18.0 39.0 76-77 30.385152660200074 34.0 29.5 36.0 18.0 38.5 78-79 30.98028407094337 34.0 31.0 36.0 18.0 39.0 80-81 30.77367601504133 34.0 31.0 36.0 14.5 37.5 82-83 30.41162425292951 34.0 31.0 35.5 9.5 37.0 84-85 30.023672402386957 34.0 30.0 35.0 7.0 37.0 86-87 29.60962097440161 34.0 30.0 35.0 2.0 36.0 88-89 29.24708088715513 34.0 29.5 35.0 2.0 36.0 90-91 28.741953385127637 34.0 29.0 35.0 2.0 35.5 92-93 28.290217390155043 34.0 29.0 35.0 2.0 35.0 94-95 27.84114295220476 33.0 27.5 35.0 2.0 35.0 96-97 27.32487772486659 33.0 27.0 35.0 2.0 35.0 98-99 26.494022202492626 33.0 25.5 34.5 2.0 35.0 100-101 23.918661879870253 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2759305436725441 1101 2 0.23972198504450404 1101 3 0.18415124198713784 1101 4 0.15162668855101202 1101 5 0.09403314955684294 1101 6 0.10457248966298494 1101 7 0.15506825885310604 1101 8 0.14651439661660248 1101 9 0.2525412704639294 1101 10-11 0.20460084572200543 1101 12-13 0.26551160580195443 1101 14-15 0.2705505532920469 1101 16-17 0.26909022699142326 1101 18-19 0.2600599026599113 1101 20-21 0.29509884150314036 1101 22-23 0.24601292622079995 1101 24-25 0.1867538674371403 1101 26-27 0.0996963299084257 1101 28-29 0.15235677246091228 1101 30-31 0.17054349146318515 1101 32-33 0.16244736786489256 1101 34-35 0.11545407148547326 1101 36-37 0.014671131062897302 1101 38-39 0.01419052511186436 1101 40-41 0.06830127091154736 1101 42-43 0.18010910258733048 1101 44-45 0.18815795693389958 1101 46-47 0.24209211950002185 1101 48-49 0.2605770746995617 1101 50-51 0.21408115808218753 1101 52-53 0.14628800984067425 1101 54-55 0.18651417224604216 1101 56-57 0.18011051409416012 1101 58-59 0.19551602583546668 1101 60-61 0.22822423649093793 1101 62-63 0.251471794025214 1101 64-65 0.3183435655908724 1101 66-67 0.11773573229609724 1101 68-69 0.1067842347662733 1101 70-71 0.1673477361142588 1101 72-73 0.084198456495578 1101 74-75 0.13284349346105273 1101 76-77 0.09391974429845362 1101 78-79 0.15458081311553684 1101 80-81 0.07685983933001594 1101 82-83 0.1818395142950493 1101 84-85 0.14719488734062836 1101 86-87 0.10200150009246656 1101 88-89 0.19001682481338023 1101 90-91 0.107026528142395 1101 92-93 0.031149566426236674 1101 94-95 -0.008642566924294215 1101 96-97 0.18304641697141122 1101 98-99 0.2677989375879051 1101 100-101 0.6176808774592324 1102 1 0.16568209646757737 1102 2 0.17139900367804017 1102 3 0.15837484447160932 1102 4 0.07740308606653912 1102 5 0.06080333589225262 1102 6 0.05488304866919691 1102 7 0.0895403085425528 1102 8 0.10024110469113623 1102 9 0.19477729530865417 1102 10-11 0.15320333019405297 1102 12-13 0.17234390393860366 1102 14-15 0.2452399942858321 1102 16-17 0.212102649351678 1102 18-19 0.2468611448959379 1102 20-21 0.17118579802487943 1102 22-23 0.20144770882949814 1102 24-25 0.10383461277875483 1102 26-27 0.09301931127488672 1102 28-29 0.0874499401627773 1102 30-31 0.09026398835759863 1102 32-33 0.06943494550463924 1102 34-35 0.06607518949790148 1102 36-37 0.019018957149853577 1102 38-39 0.061861332565278815 1102 40-41 0.0183012709115431 1102 42-43 -0.006071021636266494 1102 44-45 -0.09709049027107142 1102 46-47 -0.06800104820184316 1102 48-49 -0.04004404331286082 1102 50-51 -0.08731635744576494 1102 52-53 0.024083040896577756 1102 54-55 -2.870699899872875E-4 1102 56-57 -0.19442364739652618 1102 58-59 -0.1979622350341046 1102 60-61 -0.29304905543452975 1102 62-63 -0.20970833019838864 1102 64-65 -0.009606744968131409 1102 66-67 -0.030711472672841467 1102 68-69 -0.025824460885907286 1102 70-71 -0.14973300922735433 1102 72-73 -0.0581928478522471 1102 74-75 -0.20985836989298434 1102 76-77 -0.31042808178850123 1102 78-79 -0.09091608129440232 1102 80-81 -0.09363705507992037 1102 82-83 -0.023750547816753453 1102 84-85 0.06318867616050383 1102 86-87 -0.005451916056603778 1102 88-89 0.09886775649039592 1102 90-91 0.17690230453991163 1102 92-93 0.26732969064984147 1102 94-95 0.46340712251669913 1102 96-97 0.4811830629341465 1102 98-99 0.546215086656229 1102 100-101 0.9620908153474304 1103 1 0.19905165669045033 1103 2 0.1980894975158094 1103 3 0.17330764825305778 1103 4 0.141863308742046 1103 5 0.09683767019318879 1103 6 0.1222498798885212 1103 7 0.12968932492256613 1103 8 0.156483650375975 1103 9 0.19915978333679618 1103 10-11 0.1518249936076259 1103 12-13 0.16154985580345027 1103 14-15 0.2744436286952521 1103 16-17 0.2708596341823295 1103 18-19 0.1715738603343837 1103 20-21 0.12126600995218695 1103 22-23 0.17006281943805135 1103 24-25 0.20978223229253246 1103 26-27 0.09065665547095847 1103 28-29 0.16434705712834585 1103 30-31 0.25193162729005536 1103 32-33 0.3279475986115301 1103 34-35 0.2770523365903941 1103 36-37 0.21108237152087383 1103 38-39 0.15085466788310242 1103 40-41 0.19580542135435763 1103 42-43 0.31404758813020095 1103 44-45 0.44851858096870956 1103 46-47 0.47760138033162747 1103 48-49 0.32489105794596185 1103 50-51 0.2938599110114666 1103 52-53 0.2566210142018832 1103 54-55 0.2821087593893523 1103 56-57 0.3465867232165465 1103 58-59 0.4285369188948849 1103 60-61 0.40085643633550916 1103 62-63 0.48477093157217865 1103 64-65 0.500726109557597 1103 66-67 0.456096112598523 1103 68-69 0.4589747795481003 1103 70-71 0.5183387479832291 1103 72-73 0.47963851331319773 1103 74-75 0.47123508948861925 1103 76-77 0.3521303790215278 1103 78-79 0.4258662916600926 1103 80-81 0.33061876203288776 1103 82-83 0.3191264232689939 1103 84-85 0.3425658700016676 1103 86-87 0.4450022352852194 1103 88-89 0.27612447860950695 1103 90-91 0.3046301593553231 1103 92-93 0.3783024425682342 1103 94-95 0.3756160171503886 1103 96-97 0.48156759072263355 1103 98-99 0.571402381007033 1103 100-101 0.9110993040268589 1104 1 0.23582185336194073 1104 2 0.24459285787875729 1104 3 0.08077205659826348 1104 4 0.046162367048196984 1104 5 0.15331263556940655 1104 6 0.17547053614320873 1104 7 0.13425279307870142 1104 8 0.13472939275999352 1104 9 0.23896908338925726 1104 10-11 0.22585560239203062 1104 12-13 0.27477955401861465 1104 14-15 0.23903426256522664 1104 16-17 0.3124798711786241 1104 18-19 0.26621382113043524 1104 20-21 0.1703388383409319 1104 22-23 0.19419380040113765 1104 24-25 0.15166395809430355 1104 26-27 0.1917724280822526 1104 28-29 0.13152928814911036 1104 30-31 0.23549754325934913 1104 32-33 0.2262405357804127 1104 34-35 0.11354510042184529 1104 36-37 0.18120180130200936 1104 38-39 0.19725966428546116 1104 40-41 0.17881567499506446 1104 42-43 0.18279233453762345 1104 44-45 0.22578025222883014 1104 46-47 0.22372410868013048 1104 48-49 0.21916578859854496 1104 50-51 0.26352707680055687 1104 52-53 0.2327194016193559 1104 54-55 0.21306461698534207 1104 56-57 0.27245775047155263 1104 58-59 0.33477321612576816 1104 60-61 0.27207560617687676 1104 62-63 0.2835157786671161 1104 64-65 0.26035695370737244 1104 66-67 0.23077055721004314 1104 68-69 0.2765177650967914 1104 70-71 0.3235932091318361 1104 72-73 0.22994619113482173 1104 74-75 0.22939395956663944 1104 76-77 0.24318404956289186 1104 78-79 0.39870887624009654 1104 80-81 0.39350038570875157 1104 82-83 0.43518488753205986 1104 84-85 0.5056704667409626 1104 86-87 0.4714385731673083 1104 88-89 0.48849276744061854 1104 90-91 0.49759032040224227 1104 92-93 0.4982648717398206 1104 94-95 0.538396920113847 1104 96-97 0.6112053159238222 1104 98-99 0.6623674133382345 1104 100-101 0.8962116032506948 1105 1 0.10810084040073065 1105 2 0.051897124674454176 1105 3 0.0419650207828397 1105 4 -0.017207969707193627 1105 5 -0.08673735822880246 1105 6 -0.06722321178586554 1105 7 0.04365783244512755 1105 8 0.03513463487045243 1105 9 0.1302048135647027 1105 10-11 0.06383799352779107 1105 12-13 0.04422514921994747 1105 14-15 0.008885345840319303 1105 16-17 0.09189558886616567 1105 18-19 0.04936449157369083 1105 20-21 0.07959489422098187 1105 22-23 0.06682820441272952 1105 24-25 -0.01470825536107867 1105 26-27 -0.1284675758812739 1105 28-29 0.02523255731764351 1105 30-31 0.008266629748128196 1105 32-33 0.020784402105682886 1105 34-35 -0.03800659421260377 1105 36-37 -0.10701079391653678 1105 38-39 -0.022937401512884037 1105 40-41 -0.011434304537274897 1105 42-43 -0.052735975311485106 1105 44-45 -0.02563342185503359 1105 46-47 -0.14193301917827483 1105 48-49 -0.19362493563337324 1105 50-51 -0.1333129272332485 1105 52-53 -0.16141500608168968 1105 54-55 -0.19081327558702554 1105 56-57 -0.25628780505940796 1105 58-59 -0.32636929044408447 1105 60-61 -0.23526604704087362 1105 62-63 -0.27009508560224305 1105 64-65 -0.22125149168859792 1105 66-67 -0.1924788780406388 1105 68-69 -0.21038750002881557 1105 70-71 -0.2408156198263285 1105 72-73 -0.26128416298557156 1105 74-75 -0.277468767962592 1105 76-77 -0.3966207890974758 1105 78-79 -0.23994380003389892 1105 80-81 -0.3253461658681722 1105 82-83 -0.1674199341217033 1105 84-85 -0.13323291641399493 1105 86-87 -0.10939375259492223 1105 88-89 -0.2508293506688517 1105 90-91 -0.18967120499891266 1105 92-93 -0.06710778198987555 1105 94-95 -0.04970836482937102 1105 96-97 0.11143739986898993 1105 98-99 0.37001630985901457 1105 100-101 0.6333947190646221 1106 1 0.11423876535492639 1106 2 0.10380480975194217 1106 3 0.09127794871580619 1106 4 0.08139021956990433 1106 5 0.03367501412216711 1106 6 0.08765582804332439 1106 7 0.03687324708798201 1106 8 0.08227186225771987 1106 9 0.06570329725118995 1106 10-11 -0.031424896243727574 1106 12-13 -0.04716478700303384 1106 14-15 0.16722418482483192 1106 16-17 0.15362749519373153 1106 18-19 0.12427731415228749 1106 20-21 0.10795849664693691 1106 22-23 0.14422438000482884 1106 24-25 0.1743154128523301 1106 26-27 0.09708355179044759 1106 28-29 0.15857518662482306 1106 30-31 0.18874242867621405 1106 32-33 0.18501751561874613 1106 34-35 0.1259660844775965 1106 36-37 0.1503277687709712 1106 38-39 0.16555377708748864 1106 40-41 0.19120833425032657 1106 42-43 -0.05229810543225 1106 44-45 0.044324499107787574 1106 46-47 0.15579378321699267 1106 48-49 0.15549126440861016 1106 50-51 0.33582314848614914 1106 52-53 0.3248590458673135 1106 54-55 0.3336753444072116 1106 56-57 0.15922488818710434 1106 58-59 0.18524901686974005 1106 60-61 0.20042056944966902 1106 62-63 0.18968894531645475 1106 64-65 0.045343020514401644 1106 66-67 0.11917233406951055 1106 68-69 0.1791495862957433 1106 70-71 0.281656105842103 1106 72-73 0.2551250752942167 1106 74-75 0.028352724992942058 1106 76-77 -0.005789828635247574 1106 78-79 0.16473465702626555 1106 80-81 0.08954974689132555 1106 82-83 0.04616779165149154 1106 84-85 0.13481907168863216 1106 86-87 0.20421606956055527 1106 88-89 -0.05250048641183369 1106 90-91 0.15231789731619116 1106 92-93 0.21014556022627318 1106 94-95 0.46975120709103635 1106 96-97 0.487537746217793 1106 98-99 0.7200495650987904 1106 100-101 0.7782660159378203 1107 1 0.30385175500317985 1107 2 0.30243232731163516 1107 3 0.28912921688152693 1107 4 0.12715524091457553 1107 5 0.19595620718335027 1107 6 0.17221464942778653 1107 7 0.17663781102601916 1107 8 0.18351406548745786 1107 9 0.30650980316043075 1107 10-11 0.3274846294244327 1107 12-13 0.30619583903728653 1107 14-15 0.3561090577923878 1107 16-17 0.3916862795305889 1107 18-19 0.42488470523669974 1107 20-21 0.4032541511089036 1107 22-23 0.39090701506376035 1107 24-25 0.3484096135313237 1107 26-27 0.3809599388147973 1107 28-29 0.4622495066846142 1107 30-31 0.4241495380821618 1107 32-33 0.3146160129446116 1107 34-35 0.3393159404586754 1107 36-37 0.32380477679484443 1107 38-39 0.44263251228813516 1107 40-41 0.46920159100305625 1107 42-43 0.47648248582417096 1107 44-45 0.44444941079154887 1107 46-47 0.2966775056668496 1107 48-49 0.32717467250809307 1107 50-51 0.44832417677593384 1107 52-53 0.3473782274828352 1107 54-55 0.28576411454928774 1107 56-57 0.29091460604772124 1107 58-59 0.18373334690029708 1107 60-61 0.31862124027383487 1107 62-63 0.48662767558131037 1107 64-65 0.39865266963567336 1107 66-67 0.46215219242514394 1107 68-69 0.52392041915504 1107 70-71 0.4340027798132766 1107 72-73 0.5958720133243673 1107 74-75 0.5483649215307658 1107 76-77 0.4410103231724136 1107 78-79 0.5868089611001608 1107 80-81 0.6278602507329012 1107 82-83 0.6005794173645143 1107 84-85 0.6240459494624631 1107 86-87 0.6923776828757546 1107 88-89 0.7011971998923947 1107 90-91 0.7886099777054092 1107 92-93 0.9079885874880631 1107 94-95 1.0043542522290174 1107 96-97 1.0449791074459291 1107 98-99 1.1525298700676636 1107 100-101 1.5794434879610435 1108 1 0.09751235375457412 1108 2 0.05800076743861382 1108 3 0.039117387206999865 1108 4 0.026497298683423764 1108 5 -0.00289531341514504 1108 6 -0.05721020999624926 1108 7 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2316 60-61 -0.15692865409673828 2316 62-63 -0.06800238631853261 2316 64-65 -0.23812232457066784 2316 66-67 -0.25631935480246426 2316 68-69 -0.0407280649199393 2316 70-71 0.05663829279611576 2316 72-73 0.025379484633127447 2316 74-75 -0.03409908217717472 2316 76-77 -0.021793508371317216 2316 78-79 -0.02268542828114306 2316 80-81 -0.033881240976281646 2316 82-83 -0.07975092229538916 2316 84-85 -0.12346277462138033 2316 86-87 -0.04582297273200808 2316 88-89 0.07457119523731492 2316 90-91 0.120161689103643 2316 92-93 -0.03641993809978672 2316 94-95 0.08199901828250589 2316 96-97 0.3655098407391826 2316 98-99 0.558432774960874 2316 100-101 0.8706486206443671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 24.0 5 67.0 6 129.0 7 286.0 8 733.0 9 1459.0 10 2501.0 11 4266.0 12 6798.0 13 9827.0 14 12744.0 15 17527.0 16 24751.0 17 36214.0 18 32666.0 19 17823.0 20 13948.0 21 13885.0 22 14510.0 23 15566.0 24 17950.0 25 21046.0 26 25187.0 27 31381.0 28 39154.0 29 49110.0 30 61691.0 31 77883.0 32 100255.0 33 132040.0 34 181777.0 35 263767.0 36 405421.0 37 626942.0 38 528457.0 39 49442.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88816101885823 20.311557516127657 15.387224153533808 21.41305731148031 2 15.150097858876679 21.75064648890527 40.11082566246389 22.98842998975416 3 18.863554097648812 25.97533737786144 31.291754794873484 23.869353729616257 4 12.295660050506742 17.99192526553047 38.21703800693983 31.495376677022957 5 12.584372251413182 38.86099443540611 34.544159820353684 14.010473492827014 6 29.99634505113933 38.14892524413614 17.508156668660384 14.346573036064159 7 26.83165982259576 34.854607403157075 21.0765075606688 17.237225213578363 8 25.949678720478886 35.28872509955947 19.958346976243536 18.80324920371811 9 26.37653586273182 17.049367186313223 20.980851522020096 35.593245428934864 10-11 24.21761361093087 27.810019494235437 27.43396860832447 20.53839828650922 12-13 25.63258318672234 25.52566275424003 28.251878244996355 20.589875814041278 14-15 22.28186528570457 26.607005588864425 27.66482603395049 23.446303091480512 16-17 20.627375320248593 29.92275263820694 28.166174347052376 21.283697694492094 18-19 20.557060730306013 27.92463756265312 31.486838130879597 20.031463576161272 20-21 21.641561949620918 26.791920624520444 31.542790216861473 20.023727208997165 22-23 21.154066100960428 26.916713029530133 31.421315333087435 20.507905536422 24-25 21.074621931314454 27.00591312218279 31.747054981779886 20.17240996472287 26-27 21.021973498151016 27.157081751394667 31.64213340025292 20.178811350201393 28-29 20.527707349791598 27.56111491667978 31.697827782969686 20.213349950558936 30-31 20.785468600041803 27.18911875552692 31.559055972880774 20.466356671550507 32-33 20.673846391345986 27.410512788972696 31.39981721731156 20.51582360236976 34-35 21.168304227087575 27.170544425251364 31.36458943152328 20.29656191613778 36-37 21.181115933074608 27.01474209701017 31.319316704314637 20.484825265600588 38-39 20.872141506235273 27.440799987029674 31.344391709425267 20.342666797309786 40-41 20.980723120025743 27.469844739575493 31.17532116678468 20.374110973614084 42-43 20.888130848155107 27.601467898057898 30.721799971129904 20.788601282657094 44-45 21.125290928869994 27.523884495841656 30.513506307255074 20.837318268033272 46-47 21.387795008474757 27.98968296402607 29.802812513106886 20.819709514392287 48-49 21.512281791270347 28.057918074867594 29.559390099353283 20.87041003450878 50-51 21.322961079552762 28.240031100548453 29.53214539858856 20.90486242131022 52-53 21.626374989712744 28.68204882300581 28.80699381813049 20.88458236915096 54-55 21.27858711026068 28.40046820000641 28.96259722005365 21.358347469679256 56-57 21.459484169348546 28.661583808823266 28.854816760883516 21.02411526094467 58-59 21.7045631173013 28.253318619550132 28.93025955776176 21.1118587053868 60-61 21.45773952162817 28.379902504151026 29.407993447173652 20.75436452704715 62-63 21.330556465082903 28.341925576500337 29.417844538847316 20.90967341956945 64-65 21.22201822963018 28.46336363146132 29.253706671497635 21.06091146741086 66-67 21.225102202873273 28.453265821928213 28.925554295728013 21.3960776794705 68-69 21.201891483092133 28.447301598434677 29.039337286582107 21.311469631891082 70-71 21.40050474958397 28.556751373734095 28.88559109291978 21.157152783762157 72-73 21.502395454188196 28.28206125017579 28.909588125680898 21.30595516995512 74-75 21.10678336656388 29.00742937965614 28.400750163274356 21.485037090505628 76-77 21.293918862954925 28.606565726166437 28.641317698940068 21.45819771193857 78-79 21.299575751017976 28.690150212406458 28.453071972181505 21.557202064394065 80-81 21.240290323683485 28.929240210669004 28.448210536481316 21.382258929166195 82-83 21.405493997136247 28.817534970811764 28.44041854829827 21.336552483753717 84-85 21.186151096727784 28.660962405129926 28.355066049328652 21.797820448813642 86-87 21.180710610876943 28.660156369779315 28.337643259368495 21.821489759975243 88-89 21.28318663606895 28.477919985168732 28.318645984191026 21.92024739457129 90-91 21.214289025173244 28.304958775421795 28.509314810692644 21.97143738871232 92-93 21.27010142130817 28.370438904896595 28.562866231374517 21.79659344242072 94-95 21.06586344730406 28.27383144729278 28.62598594624582 22.034319159157338 96-97 20.89402445819877 28.319139419909924 28.736850378659046 22.049985743232263 98-99 20.925785432004123 28.43273106966785 28.211069844081116 22.430413654246905 100-101 20.731937097657475 28.29858078612241 28.542539842738858 22.426942273481256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1299.0 1 918.5 2 644.0 3 792.5 4 761.5 5 783.0 6 1361.5 7 2852.5 8 4655.5 9 5587.0 10 5301.0 11 4938.0 12 5263.5 13 5674.5 14 6080.0 15 6386.5 16 6706.0 17 7068.5 18 7440.0 19 8030.5 20 8654.5 21 9816.5 22 11434.5 23 13173.5 24 15385.0 25 18739.5 26 23133.0 27 27616.5 28 31242.0 29 35775.5 30 41430.0 31 46216.0 32 52473.0 33 60390.0 34 67707.5 35 73809.0 36 80872.0 37 87029.0 38 89554.0 39 90592.5 40 92277.0 41 95146.0 42 98728.5 43 101941.0 44 103839.0 45 104412.0 46 103187.5 47 107636.0 48 122578.5 49 119193.0 50 100435.0 51 89611.5 52 81744.5 53 76342.0 54 71608.5 55 65621.5 56 58005.0 57 50776.0 58 45546.5 59 40197.0 60 34111.0 61 27874.5 62 21934.5 63 16453.0 64 11620.0 65 8628.0 66 6111.0 67 4451.0 68 3087.0 69 1825.5 70 1298.0 71 978.5 72 803.0 73 677.5 74 507.5 75 400.0 76 289.0 77 163.0 78 97.5 79 60.5 80 42.0 81 27.0 82 15.5 83 15.0 84 9.0 85 4.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02333246042204967 2 0.0 3 0.0 4 1.4098163397008862E-4 5 0.019878410389782497 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0032602002855582997 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010044941420368815 24-25 0.0 26-27 4.5819031040278803E-4 28-29 0.00114547577600697 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.0573622547756649E-4 40-41 5.639265358803545E-4 42-43 0.014221522326732692 44-45 1.7622704246261078E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.7622704246261078E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.586043382163497E-4 70-71 1.7622704246261078E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.049081698504431E-5 82-83 0.0021675926222901125 84-85 4.758130146490491E-4 86-87 0.0 88-89 0.0 90-91 0.010168300350092644 92-93 0.0026081602284466396 94-95 0.0 96-97 0.0 98-99 0.0012864574099770588 100-101 0.017710817767492382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2837249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.83553735954191 #Duplication Level Percentage of deduplicated Percentage of total 1 65.25134718633122 26.645738257878687 2 16.170960766537387 13.206997450432482 3 6.748890419187875 8.26783700544603 4 3.6742027310336796 6.001521715586271 5 2.159081371579262 4.408362400570798 6 1.4140991485889738 3.4647299167380874 7 0.9099029367117799 2.6009462755955557 8 0.6518830478926716 2.1295995645018593 9 0.5004318481878175 1.8391863089320406 >10 2.27414145519054 16.236633156575213 >50 0.15166591455407832 4.311154689606257 >100 0.08700904832841114 6.262242652467057 >500 0.0038822387085780076 1.0538941741873733 >1k 0.0021567992825112206 1.5882923265423443 >5k 8.627197130044883E-5 0.299874551582123 >10k+ 2.588159139013465E-4 1.6829895533577517 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19864 0.7001147942954602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16692 0.5883163585571799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11216 0.3953125016521285 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8512 0.30000891708834865 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3785 0.13340387114419638 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3285 0.1157811668979353 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2920 0.10291659279816472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2335892972382755E-4 0.0 24-25 0.0 0.0 0.0 3.7007678917148266E-4 0.0 26-27 0.0 0.0 0.0 7.930216910817486E-4 0.0 28-29 0.0 0.0 0.0 0.002238083439275157 0.0 30-31 0.0 0.0 0.0 0.0048638663719680585 0.0 32-33 0.0 0.0 0.0 0.009110938095316979 0.0 34-35 0.0 0.0 0.0 0.01355185956537477 0.0 36-37 0.0 0.0 0.0 0.018556707571312916 0.0 38-39 0.0 0.0 0.0 0.02368491450697489 0.0 40-41 0.0 0.0 0.0 0.030170069669598967 0.0 42-43 0.0 0.0 0.0 0.039985915934766386 0.0 44-45 0.0 0.0 0.0 0.05464800586765561 0.0 46-47 0.0 0.0 0.0 0.08696804545529843 0.0 48-49 0.0 0.0 0.0 0.14061155718091717 0.0 50-51 0.0 0.0 0.0 0.18431586371164463 0.0 52-53 0.0 0.0 0.0 0.22876032382071507 0.0 54-55 0.0 0.0 0.0 0.29066888383783024 0.0 56-57 0.0 0.0 0.0 0.3421448029411589 0.0 58-59 0.0 0.0 0.0 0.40285501906952825 0.0 60-61 0.0 0.0 0.0 0.4917263165834229 0.0 62-63 0.0 0.0 0.0 0.5556438648846118 0.0 64-65 0.0 0.0 0.0 0.6302583946632812 0.0 66-67 0.0 0.0 0.0 0.7576529236595027 0.0 68-69 0.0 0.0 0.0 0.9007492821391425 0.0 70-71 0.0 0.0 0.0 1.0274389029655135 0.0 72-73 0.0 0.0 0.0 1.1744651244920696 0.0 74-75 0.0 0.0 0.0 1.3165393661254265 0.0 76-77 0.0 0.0 0.0 1.4399864093704853 0.0 78-79 0.0 0.0 0.0 1.6016042300129456 0.0 80-81 0.0 0.0 0.0 1.8249015155173198 0.0 82-83 0.0 0.0 0.0 2.032884671031693 0.0 84-85 0.0 0.0 0.0 2.2691698895655614 0.0 86-87 0.0 0.0 0.0 2.6484457303535924 0.0 88-89 0.0 0.0 0.0 3.133686891774391 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 28200 0.0 23.97753 1 GTACATG 14115 0.0 23.665903 1 GGTATCA 21265 0.0 23.328337 1 TACATGG 14505 0.0 22.729952 2 GAGTACT 16675 0.0 22.34678 12-13 AGTACTT 16920 0.0 21.99513 12-13 ACATGGG 14340 0.0 21.865107 3 GTACTTT 17550 0.0 21.218346 14-15 CGTATAG 250 9.464202E-9 20.907463 1 ATCAACG 33195 0.0 20.293657 3 TCAACGC 33260 0.0 20.253998 4 CAACGCA 33535 0.0 20.087908 5 TATCAAC 33930 0.0 19.93806 2 CATGGGA 9745 0.0 19.890015 4 AACGCAG 34110 0.0 19.773302 6 AGAGTAC 26825 0.0 19.74345 10-11 GTAGCGG 170 7.962073E-5 19.557875 6 TACTTTT 20030 0.0 18.460789 14-15 ACTTTTT 20770 0.0 18.04334 16-17 ATGGGAG 4110 0.0 17.916231 5 >>END_MODULE