FastQCFastQC Report
Thu 26 May 2016
SRR937781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613523
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180700.6914039019361988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139710.5345657949059565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94820.3628053015029904No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54010.20665591999764304No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44510.1703065172948545No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34360.131470050196612No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34160.1307047996133954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31850.12186615537724367No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26430.10112786457207379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA223200.028.2412451
GTACATG124250.026.5137631
GGTATCA158200.026.1425111
TACATGG128700.025.4424292
ACATGGG130300.024.1798533
GAGTACT142300.023.5638912-13
ATCAACG267750.023.356673
TCAACGC268000.023.2989734
TATCAAC269400.023.2330422
CAACGCA269150.023.1994255
AACGCAG273200.022.9198076
AGTACTT147050.022.88347612-13
CATGGGA88150.022.1562234
GTACTTT151600.022.00932914-15
AGAGTAC225450.021.03653310-11
ACGCAGA297000.020.9555687
CGCAGAG297150.020.8970248
GTGTAGC12550.020.0755141
GCAGAGT306250.019.8246739
CATGGGG41450.019.0309184
Produced by FastQC (version 0.11.5)