##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937781_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2613523 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884162106092045 33.0 31.0 34.0 30.0 34.0 2 32.01170986442438 33.0 31.0 34.0 30.0 34.0 3 32.026789127166666 34.0 31.0 34.0 30.0 34.0 4 35.631445753490596 37.0 35.0 37.0 33.0 37.0 5 35.633396377227214 37.0 35.0 37.0 33.0 37.0 6 35.69067653125685 37.0 35.0 37.0 33.0 37.0 7 35.63970586828584 37.0 35.0 37.0 33.0 37.0 8 35.631303034256824 37.0 35.0 37.0 33.0 37.0 9 37.272830964181296 39.0 37.0 39.0 34.0 39.0 10-11 37.16878462519748 39.0 37.0 39.0 34.0 39.0 12-13 37.05902416010879 39.0 37.0 39.0 33.0 39.0 14-15 38.2182550144001 40.0 38.0 41.0 33.0 41.0 16-17 38.16735322398158 40.0 38.0 41.0 33.0 41.0 18-19 38.15282762003625 40.0 38.0 41.0 33.5 41.0 20-21 38.09755127465877 40.0 38.0 41.0 33.0 41.0 22-23 37.915071725024035 40.0 38.0 41.0 33.0 41.0 24-25 37.75853761378798 40.0 37.5 41.0 33.0 41.0 26-27 37.70114917680081 40.0 37.0 41.0 32.5 41.0 28-29 37.74059172236096 40.0 37.0 41.0 33.0 41.0 30-31 37.5540630405778 40.0 37.0 41.0 33.0 41.0 32-33 37.291757715543355 39.5 36.5 41.0 32.0 41.0 34-35 37.295065893814595 40.0 36.0 41.0 32.0 41.0 36-37 37.20581930979755 40.0 36.0 41.0 32.0 41.0 38-39 37.073137485302404 40.0 36.0 41.0 31.0 41.0 40-41 36.845875471537845 39.5 36.0 41.0 31.0 41.0 42-43 36.581066246595114 39.0 36.0 41.0 30.0 41.0 44-45 36.30314177453192 39.0 35.5 41.0 30.0 41.0 46-47 35.89123359541891 39.0 35.0 41.0 28.5 41.0 48-49 35.48863009814721 39.0 35.0 41.0 27.0 41.0 50-51 34.53718945653051 38.0 34.0 39.5 25.0 40.5 52-53 34.632603577623 38.0 34.5 39.5 25.5 40.5 54-55 35.01127367159195 39.0 35.0 40.0 26.0 41.0 56-57 34.836552041057224 39.0 35.0 40.0 24.5 41.0 58-59 34.621946698001125 38.0 34.0 40.0 24.0 41.0 60-61 34.31844315125599 38.0 34.0 40.0 23.0 41.0 62-63 33.995347659079336 38.0 34.0 40.0 22.0 41.0 64-65 33.65409525762735 37.0 33.5 40.0 21.5 41.0 66-67 33.25955903200392 37.0 33.0 39.5 20.0 41.0 68-69 32.79991586069838 36.0 32.5 39.0 18.0 41.0 70-71 32.36871112287896 36.0 32.0 39.0 16.0 40.0 72-73 31.930633095633752 35.0 31.5 38.5 12.0 40.0 74-75 31.490090387572636 35.0 31.0 37.5 8.0 39.5 76-77 31.014768379692853 35.0 31.0 37.0 7.0 39.0 78-79 30.49830095239261 35.0 30.5 36.5 2.0 39.0 80-81 29.977336912665393 34.0 30.0 36.0 2.0 38.0 82-83 29.469217221352174 34.0 29.0 35.5 2.0 37.0 84-85 29.01588698473287 34.0 29.0 35.0 2.0 37.0 86-87 28.574528902175338 34.0 29.0 35.0 2.0 36.0 88-89 28.11089915795652 34.0 27.5 35.0 2.0 36.0 90-91 27.631288111870454 33.5 26.5 35.0 2.0 35.5 92-93 27.111699992691857 33.0 26.0 35.0 2.0 35.0 94-95 26.523469661449315 33.0 25.0 35.0 2.0 35.0 96-97 25.86913870664234 32.0 23.5 35.0 2.0 35.0 98-99 24.998279716688927 32.0 19.0 34.0 2.0 35.0 100-101 22.677792772437815 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16053279767438866 1101 2 0.21829944519566524 1101 3 0.22903724872361408 1101 4 0.14492142667040042 1101 5 0.1617114645723987 1101 6 0.19971162903676998 1101 7 0.10704853622310395 1101 8 0.15134757653242303 1101 9 0.2514259837994075 1101 10-11 0.20632651292621773 1101 12-13 0.3221717348341713 1101 14-15 0.48677297064801905 1101 16-17 0.5190276039149779 1101 18-19 0.3995265399014798 1101 20-21 0.4754717880057342 1101 22-23 0.38910981391156696 1101 24-25 0.37251400691081926 1101 26-27 0.4924686662407538 1101 28-29 0.27490539910535716 1101 30-31 0.3615074982821298 1101 32-33 0.29775750233461906 1101 34-35 0.42882230979709846 1101 36-37 0.37571849583117256 1101 38-39 0.4973650428913672 1101 40-41 0.5355757353947581 1101 42-43 0.493745401656156 1101 44-45 0.536375840983851 1101 46-47 0.6234260453173945 1101 48-49 0.6425797474359811 1101 50-51 0.5622809686060393 1101 52-53 0.5636198751416259 1101 54-55 0.6316711519780398 1101 56-57 0.5265734940394893 1101 58-59 0.5750182526757825 1101 60-61 0.5935021126533897 1101 62-63 0.7440382606053717 1101 64-65 0.6701143792122508 1101 66-67 0.7926890460786851 1101 68-69 0.7255555944822234 1101 70-71 0.8278255129275109 1101 72-73 0.8072122111895332 1101 74-75 0.8601902065484879 1101 76-77 0.8601534141558602 1101 78-79 0.7851337755056242 1101 80-81 0.7713797629963501 1101 82-83 0.8827782875826493 1101 84-85 0.9286183993885011 1101 86-87 1.0518943300236536 1101 88-89 0.9694846146293585 1101 90-91 1.1318442865305691 1101 92-93 1.1989070771584096 1101 94-95 1.2723043625989412 1101 96-97 1.3744695722507423 1101 98-99 1.6233961550812595 1101 100-101 2.1556885059894277 1102 1 0.296902485822347 1102 2 0.17545727415656032 1102 3 0.23882499080684738 1102 4 0.1547848551518598 1102 5 0.14451035445935645 1102 6 0.07900157135799901 1102 7 0.12024331299008395 1102 8 0.20226663302577919 1102 9 0.337250345714331 1102 10-11 0.24305270694508607 1102 12-13 0.2950192394708182 1102 14-15 0.44518214805957257 1102 16-17 0.38753223448910035 1102 18-19 0.4221146294082203 1102 20-21 0.5108595368778026 1102 22-23 0.54720447993558 1102 24-25 0.5399416085705582 1102 26-27 0.44002533155767765 1102 28-29 0.30317558762067875 1102 30-31 0.5524275845692088 1102 32-33 0.5205294639184928 1102 34-35 0.6763450997169969 1102 36-37 0.7049241108903814 1102 38-39 0.7931199648294509 1102 40-41 0.8116363111182565 1102 42-43 0.8810728381016517 1102 44-45 0.9108141131642924 1102 46-47 0.9181538684286465 1102 48-49 0.8591031661263671 1102 50-51 1.0045633813920958 1102 52-53 0.912071853211387 1102 54-55 0.9055438747491991 1102 56-57 0.9794356038574747 1102 58-59 0.959430214658731 1102 60-61 1.0332471962771166 1102 62-63 1.135820185615522 1102 64-65 0.9588680226235766 1102 66-67 0.9720512459749457 1102 68-69 0.9321573864379715 1102 70-71 1.0496170489634764 1102 72-73 1.1210098530536463 1102 74-75 1.2075166934737211 1102 76-77 1.169737903856948 1102 78-79 1.2526813401065446 1102 80-81 1.214238137805257 1102 82-83 1.119903887899067 1102 84-85 1.2976941182401376 1102 86-87 1.348225787366399 1102 88-89 1.4995090074380926 1102 90-91 1.560591104404896 1102 92-93 1.6662905972249966 1102 94-95 1.8301843888781768 1102 96-97 1.8555891415079806 1102 98-99 1.9061724078545659 1102 100-101 2.5144755190750345 1103 1 0.23245147268709943 1103 2 0.22797587656095786 1103 3 0.26647107486476074 1103 4 0.17297387278424026 1103 5 0.18285153254409892 1103 6 0.1872847331162717 1103 7 0.18957747949502135 1103 8 0.21324608596189165 1103 9 0.26527661763395827 1103 10-11 0.2364857933837996 1103 12-13 0.25080960439571953 1103 14-15 0.3982039235539503 1103 16-17 0.34886315503708687 1103 18-19 0.2270364200447048 1103 20-21 0.15742736038760086 1103 22-23 0.27131680028295335 1103 24-25 0.39761357639819295 1103 26-27 0.33679682713625425 1103 28-29 0.20710030928662349 1103 30-31 0.2673083706509729 1103 32-33 0.3230535757767541 1103 34-35 0.3663331068799991 1103 36-37 0.3873842491030288 1103 38-39 0.47156228937823386 1103 40-41 0.39176243140349953 1103 42-43 0.4658679539530226 1103 44-45 0.3593472832756106 1103 46-47 0.5127942006167174 1103 48-49 0.47443327482967135 1103 50-51 0.48207610112155663 1103 52-53 0.46888996232415536 1103 54-55 0.601876106902651 1103 56-57 0.5472139716799873 1103 58-59 0.4348816877445145 1103 60-61 0.49613942496139174 1103 62-63 0.742014553460784 1103 64-65 0.579848698302996 1103 66-67 0.5787746991157974 1103 68-69 0.6989144792377928 1103 70-71 0.8238513592829264 1103 72-73 0.7361071428999715 1103 74-75 0.7635467729591454 1103 76-77 0.8148242174493738 1103 78-79 0.9868628094316811 1103 80-81 0.8002707480849729 1103 82-83 0.8636012503990891 1103 84-85 0.953072802554118 1103 86-87 1.1883478342102656 1103 88-89 1.0950953661724974 1103 90-91 1.1979940402684548 1103 92-93 1.3313463706705662 1103 94-95 1.345870870384541 1103 96-97 1.6393871528439483 1103 98-99 1.5747711155855058 1103 100-101 2.1693142769100113 1104 1 0.2014094943978968 1104 2 0.2074481519959157 1104 3 0.1990906285775509 1104 4 0.17539201507170077 1104 5 0.16502283576534182 1104 6 0.12013536131814817 1104 7 0.17167054298388962 1104 8 0.19320189266213106 1104 9 0.30026897346632353 1104 10-11 0.294784842163601 1104 12-13 0.3206553311942386 1104 14-15 0.42545698486571126 1104 16-17 0.5201938330791336 1104 18-19 0.48945588414627395 1104 20-21 0.5848700923052377 1104 22-23 0.45743017111305306 1104 24-25 0.4488467512110219 1104 26-27 0.43287691935222483 1104 28-29 0.40431133010617515 1104 30-31 0.4611637087875451 1104 32-33 0.44104747502147745 1104 34-35 0.3355078672282943 1104 36-37 0.3789571981772255 1104 38-39 0.4427180700309563 1104 40-41 0.4520749383891882 1104 42-43 0.49333325876239087 1104 44-45 0.4589982174385838 1104 46-47 0.4518266181078232 1104 48-49 0.4916206244854706 1104 50-51 0.3720670282283649 1104 52-53 0.3996714897775604 1104 54-55 0.48044998610633627 1104 56-57 0.5947984292842605 1104 58-59 0.4885961797839897 1104 60-61 0.6007808148489318 1104 62-63 0.6549630104857869 1104 64-65 0.45631022622292505 1104 66-67 0.5433440063977315 1104 68-69 0.48099993743591085 1104 70-71 0.4445728365268238 1104 72-73 0.6594605124082484 1104 74-75 0.7704618775039478 1104 76-77 0.8413615795943699 1104 78-79 0.7883334977177832 1104 80-81 0.7437648956558007 1104 82-83 0.911679322960854 1104 84-85 0.9212839020993542 1104 86-87 0.9551844414633308 1104 88-89 0.9609396166212818 1104 90-91 0.9287550844627255 1104 92-93 0.86089134588169 1104 94-95 0.9098540843172351 1104 96-97 1.0981765606420701 1104 98-99 1.2766028972451373 1104 100-101 1.7775198267798196 1105 1 0.3323089427108279 1105 2 0.2299166366747052 1105 3 0.30552843972074584 1105 4 0.29783981849831065 1105 5 0.32429869612838047 1105 6 0.3082553207410754 1105 7 0.2823153696362368 1105 8 0.20712629159251605 1105 9 0.3085441519345622 1105 10-11 0.41192220494948373 1105 12-13 0.3818195607372701 1105 14-15 0.4826563939582229 1105 16-17 0.4851366880507655 1105 18-19 0.43994749102601816 1105 20-21 0.4183782982486832 1105 22-23 0.3991423186838503 1105 24-25 0.46213260691759217 1105 26-27 0.4052509995975271 1105 28-29 0.5353482284181652 1105 30-31 0.48799113075088485 1105 32-33 0.5214712162829471 1105 34-35 0.5613985253715299 1105 36-37 0.5452863600688431 1105 38-39 0.6021363530739094 1105 40-41 0.7078624376305385 1105 42-43 0.7717331316657621 1105 44-45 0.7291814365022518 1105 46-47 0.7666255585952868 1105 48-49 0.6981966889069326 1105 50-51 0.6403379964934786 1105 52-53 0.6346467895792074 1105 54-55 0.7635767109253209 1105 56-57 0.6611895201782332 1105 58-59 0.6318619203589719 1105 60-61 0.6641794994090944 1105 62-63 0.8024323876970385 1105 64-65 0.8362279525806287 1105 66-67 0.8551202582000101 1105 68-69 0.9200365914490121 1105 70-71 1.04035508512721 1105 72-73 1.0114442678074305 1105 74-75 1.0082070880472571 1105 76-77 1.075723263328804 1105 78-79 1.1594226754811778 1105 80-81 1.2876315910875142 1105 82-83 1.205898058696274 1105 84-85 1.313472993516612 1105 86-87 1.384665579192312 1105 88-89 1.395302588152557 1105 90-91 1.534672676685556 1105 92-93 1.6870451480954785 1105 94-95 1.7374806288446614 1105 96-97 1.8220296309282382 1105 98-99 2.1916785610470306 1105 100-101 2.697972488643135 1106 1 0.29316065475409303 1106 2 0.3032497722989227 1106 3 0.23180318101601216 1106 4 0.22868775994628265 1106 5 0.13356824096904774 1106 6 0.23758843376996452 1106 7 0.19610854612975004 1106 8 0.09504593507590897 1106 9 0.2161000765471357 1106 10-11 0.09418694404641315 1106 12-13 0.07564416356554204 1106 14-15 0.12734698625236263 1106 16-17 0.023119198325758816 1106 18-19 0.23294604633726124 1106 20-21 0.27276812319608723 1106 22-23 0.2593026607771165 1106 24-25 0.22923621073111633 1106 26-27 0.1737724602159858 1106 28-29 0.3830914965404375 1106 30-31 0.459842295328194 1106 32-33 0.3519829580861398 1106 34-35 0.3229022538359487 1106 36-37 0.3970579514342276 1106 38-39 0.38228075604651224 1106 40-41 0.4090051021751293 1106 42-43 0.5650835621302974 1106 44-45 0.4372876985619243 1106 46-47 0.5241779291511293 1106 48-49 0.6260568690199406 1106 50-51 0.5513865467913845 1106 52-53 0.6314754412077477 1106 54-55 0.6849129609388527 1106 56-57 0.6442470257576289 1106 58-59 0.6970306109550322 1106 60-61 0.6579606062317964 1106 62-63 0.8742391618703849 1106 64-65 0.9346301334063298 1106 66-67 0.7851076942564035 1106 68-69 0.6002124395858246 1106 70-71 0.8444712477062382 1106 72-73 0.8817379082112602 1106 74-75 0.9774774425538268 1106 76-77 1.0428710692518877 1106 78-79 1.1207465357234305 1106 80-81 1.1108852900132469 1106 82-83 0.8406144614448046 1106 84-85 0.8075584311575028 1106 86-87 0.9723323903749268 1106 88-89 0.9449873597961727 1106 90-91 1.0997575762752163 1106 92-93 1.2152036336059915 1106 94-95 1.3327816009615567 1106 96-97 1.4889003616470085 1106 98-99 1.7171298438732165 1106 100-101 2.19329007774051 1107 1 0.16900171710745937 1107 2 0.2380365576937642 1107 3 0.270747509497987 1107 4 0.2038666071873365 1107 5 0.2121717350930865 1107 6 0.11356293239321502 1107 7 0.21337600207696994 1107 8 0.19725410468645066 1107 9 0.16380509811514798 1107 10-11 0.2774777132162072 1107 12-13 0.20245025608502942 1107 14-15 0.4070918621240267 1107 16-17 0.4132060874096126 1107 18-19 0.35696527721260196 1107 20-21 0.39783753764663743 1107 22-23 0.5428820736675632 1107 24-25 0.5509094212930563 1107 26-27 0.44006309745443417 1107 28-29 0.3432805847643863 1107 30-31 0.3433015152477239 1107 32-33 0.38455394894073436 1107 34-35 0.41626826400620587 1107 36-37 0.43835472122304964 1107 38-39 0.48193632994537694 1107 40-41 0.5476825079533185 1107 42-43 0.6040905956263032 1107 44-45 0.6779813966076844 1107 46-47 0.7673121063434607 1107 48-49 0.7237623169820466 1107 50-51 0.5771184271742982 1107 52-53 0.7589981266277306 1107 54-55 0.8328709596155264 1107 56-57 0.8387639990016496 1107 58-59 0.8187701383398149 1107 60-61 1.0541158357547218 1107 62-63 1.0210619489728217 1107 64-65 1.105915902227487 1107 66-67 1.004479430146901 1107 68-69 1.0868264240078886 1107 70-71 1.3895278101371034 1107 72-73 1.3144865298296473 1107 74-75 1.2158854174276712 1107 76-77 1.2775496007104863 1107 78-79 1.310315080079203 1107 80-81 1.129955389704186 1107 82-83 1.2577680952505368 1107 84-85 1.447371299329994 1107 86-87 1.4498927740493492 1107 88-89 1.6004288256688426 1107 90-91 1.7455116191182931 1107 92-93 1.7199100211400307 1107 94-95 1.7437728368071532 1107 96-97 1.7284164595290328 1107 98-99 2.1690220554589956 1107 100-101 2.6520266670191575 1108 1 0.19586671386850085 1108 2 0.08657844056779496 1108 3 0.2041827821369182 1108 4 0.22651609200879363 1108 5 0.24608959570376499 1108 6 0.2640763480357293 1108 7 0.2462402939846271 1108 8 0.19254899954066218 1108 9 0.32549101216602594 1108 10-11 0.2855897128426008 1108 12-13 0.27699638880128674 1108 14-15 0.33441471977101855 1108 16-17 0.3858987402485994 1108 18-19 0.4046847641201694 1108 20-21 0.48309657590681354 1108 22-23 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-0.7407697733074592 2316 84-85 -0.6581169438574612 2316 86-87 -0.7932403260041383 2316 88-89 -0.7998569675716354 2316 90-91 -0.8512312788582257 2316 92-93 -0.8808798837424838 2316 94-95 -0.7695229276208906 2316 96-97 -0.6497338143967717 2316 98-99 -0.8174132555822098 2316 100-101 -0.6501676850994755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8585.0 3 1337.0 4 1273.0 5 1439.0 6 1778.0 7 2027.0 8 2539.0 9 3362.0 10 4300.0 11 6115.0 12 8985.0 13 12346.0 14 14079.0 15 18412.0 16 24523.0 17 33682.0 18 28673.0 19 17071.0 20 15960.0 21 16093.0 22 17733.0 23 19569.0 24 21860.0 25 24647.0 26 28780.0 27 33857.0 28 40344.0 29 49022.0 30 59569.0 31 74973.0 32 96046.0 33 126144.0 34 172114.0 35 246654.0 36 360888.0 37 516947.0 38 442183.0 39 59606.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82474292462422 19.3140129387508 15.06786667147528 21.793377465149703 2 14.740999177166266 20.73149726968807 41.451601456933574 23.075902096212086 3 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20.320072863361837 22-23 20.999699297921016 26.456685005852094 31.783615627232148 20.760000068994742 24-25 20.8523862502767 26.49812513355968 32.10720038791001 20.54228822825361 26-27 20.756131029130426 26.700364238150375 32.0154657937576 20.528038938961597 28-29 20.321396372692963 27.01709023716536 32.10683741759541 20.554675972546274 30-31 20.59087778459552 26.68488479773255 31.938603518146536 20.785633899525397 32-33 20.46979582729168 26.810636273225057 31.816996781789697 20.902571117693565 34-35 21.003140564810952 26.711381633829774 31.63657086512746 20.64890693623181 36-37 20.9072980193883 26.50266982010817 31.741209526255464 20.848822634248062 38-39 20.695258316508593 26.93570156208493 31.697931236983745 20.671108884422733 40-41 20.76914305098649 26.95408274248216 31.631381695767622 20.64539251076373 42-43 20.675587013827887 27.007169156087418 31.132265879221677 21.18497795086302 44-45 20.813586272049353 27.042173723795354 30.958076590391116 21.186163413764174 46-47 21.137139925208288 27.295352514066813 30.402671970434447 21.16483559029046 48-49 21.20000161013835 27.406759092441686 30.14418597251302 21.249053324906942 50-51 21.01854844491577 27.647837573914476 30.17131019982356 21.162303781346196 52-53 21.228067484662578 27.961062116564417 29.57258435582822 21.238286042944786 54-55 21.022763103443054 27.582175710066338 29.744725165384565 21.65033602110605 56-57 21.247433095684688 27.940700773501497 29.491670544011246 21.32019558680257 58-59 21.41822983909527 27.50849386021681 29.716772952107256 21.356503348580663 60-61 21.272695877020684 27.579121144271408 30.146556447642354 21.001626531065558 62-63 21.083456971658666 27.630151817833166 30.147716254307856 21.138674956200312 64-65 20.986100118243858 27.76360043924623 30.019739250253448 21.230560192256466 66-67 20.94647155922476 27.717851993630315 29.69405173747083 21.641624709674094 68-69 20.888124386095207 27.741069931242663 29.831867947581514 21.538937735080616 70-71 21.138673054466935 27.76880113563733 29.597825776567916 21.49470003332782 72-73 21.222189215864773 27.487720037946662 29.726649865366 21.56344088082256 74-75 20.787987310236655 28.103038302532372 29.40124179986427 21.7077325873667 76-77 20.99805429459218 27.82709500681505 29.425540589262567 21.749310109330207 78-79 21.03743900814375 27.84303276763816 29.347704616835525 21.77182360738257 80-81 20.942370946482228 28.06995350705751 29.256053503013312 21.73162204344695 82-83 21.08972060171134 27.912036814310188 29.402725806108464 21.595516777870014 84-85 21.020454395613662 27.76459390960232 29.206045779944095 22.00890591483992 86-87 21.053391144656143 27.900862895898033 29.249148517257627 21.796597442188197 88-89 21.136290368023822 27.56966355491991 29.1869911531224 22.107054923933873 90-91 21.003959796375234 27.45835122205526 29.46381750436996 22.073871477199546 92-93 21.227045766255312 27.44787022889644 29.51883985528857 21.806244149559678 94-95 20.87880979788536 27.39755984340495 29.58512584680809 22.138504511901598 96-97 20.725536253719984 27.482153941502453 29.684066937020553 22.108242867757014 98-99 20.803260833526423 27.652479288712673 29.173367278628742 22.370892599132162 100-101 20.883877631979885 27.016498563634165 29.813454502944598 22.28616930144135 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3722.0 1 2248.0 2 812.5 3 857.0 4 748.0 5 761.5 6 1286.5 7 2787.0 8 4763.0 9 5633.0 10 5080.0 11 4528.5 12 4648.0 13 5006.5 14 5410.0 15 5626.5 16 5865.5 17 6168.5 18 6495.0 19 6890.5 20 7583.0 21 8754.0 22 10158.0 23 11937.5 24 14180.5 25 17203.5 26 21097.5 27 25248.5 28 28680.5 29 32805.0 30 37974.5 31 42785.5 32 48719.5 33 56141.5 34 63082.0 35 68626.0 36 74713.0 37 80373.5 38 82727.0 39 83456.0 40 85225.0 41 88034.5 42 91154.5 43 93929.5 44 95430.5 45 96249.5 46 95493.0 47 98922.5 48 112347.0 49 109588.0 50 92050.0 51 81698.5 52 74794.5 53 70484.0 54 66334.5 55 60494.0 56 53882.0 57 47376.0 58 41710.0 59 36202.0 60 30666.5 61 25266.0 62 19921.5 63 14836.0 64 10504.0 65 7768.0 66 5556.5 67 4057.0 68 2832.0 69 1738.0 70 1244.0 71 961.5 72 780.0 73 629.0 74 467.0 75 377.0 76 278.0 77 162.5 78 103.5 79 77.5 80 48.5 81 32.0 82 23.0 83 14.5 84 10.0 85 10.0 86 8.5 87 5.5 88 4.0 89 3.0 90 1.5 91 2.0 92 3.0 93 3.5 94 2.5 95 1.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2712430692211242 2 0.25551716973602295 3 0.2457219622708505 4 0.23485540398917476 5 0.23045521313567932 6 0.19731986288240047 7 0.18821338094212295 8 0.20091654062351852 9 0.201490478560931 10-11 0.2034609988127137 12-13 0.19969213969037194 14-15 0.19624851206589727 16-17 0.1955215240118415 18-19 0.19609546194925392 20-21 0.18383232135320793 22-23 0.17709811622090182 24-25 0.1787051424456567 26-27 0.18853861243999 28-29 0.2020070227046022 30-31 0.19638243091796015 32-33 0.20053391533191023 34-35 0.19418233549121242 36-37 0.18109655051820858 38-39 0.18235921398051597 40-41 0.17774857921663592 42-43 0.18815598714838172 44-45 0.18371753376572542 46-47 0.18410015905733373 48-49 0.19328316605593293 50-51 0.20172005373589597 52-53 0.21132394855526432 54-55 0.19060478901467484 56-57 0.16640373932044983 58-59 0.16959866050537914 60-61 0.17587371528775528 62-63 0.18256965789090052 64-65 0.1724874814570218 66-67 0.1760650279335594 68-69 0.18023564361208988 70-71 0.1756824026419511 72-73 0.1759311090814965 74-75 0.1778633668041184 76-77 0.1896864883148149 78-79 0.194392779401597 80-81 0.1854776101071236 82-83 0.1860898105736969 84-85 0.179642574410097 86-87 0.17950865555803414 88-89 0.18388971514694916 90-91 0.18377492755946667 92-93 0.18155570086813852 94-95 0.18197658868890765 96-97 0.17116742420097317 98-99 0.17675375345845434 100-101 0.1724874814570218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2613523.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.49688551475519 #Duplication Level Percentage of deduplicated Percentage of total 1 67.2972680361515 29.272215632242755 2 15.649671423847671 13.614239325332761 3 6.513942213555701 8.5000859613829 4 3.334175320257285 5.801049687654134 5 1.9805788771572783 4.307450633632627 6 1.2281001516140047 3.2051119017244716 7 0.8315071753013128 2.5317580686145074 8 0.5640443390523916 1.9627337632806112 9 0.41762153063692614 1.6348712315950082 >10 1.9646739397980129 15.045391629255334 >50 0.13774399386289252 4.174991685896596 >100 0.0753039622945963 5.641354551006716 >500 0.0029925777048727133 0.9243445883417776 >1k 0.002024390800352523 1.5879324258870164 >5k 1.7603398263934985E-4 0.5697904451738667 >10k+ 1.7603398263934985E-4 1.2266784689790942 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18070 0.6914039019361988 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13971 0.5345657949059565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9482 0.3628053015029904 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5401 0.20665591999764304 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4451 0.1703065172948545 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3436 0.131470050196612 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3416 0.1307047996133954 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3185 0.12186615537724367 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2643 0.10112786457207379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.9131264580415018E-5 0.0 16-17 0.0 0.0 0.0 3.8262529160830036E-5 0.0 18-19 0.0 0.0 0.0 3.8262529160830036E-5 0.0 20-21 0.0 0.0 0.0 1.147875874824901E-4 0.0 22-23 0.0 0.0 0.0 3.4436276244747036E-4 0.0 24-25 0.0 0.0 0.0 6.695942603145257E-4 0.0 26-27 0.0 0.0 0.0 0.0011670071394053161 0.0 28-29 0.0 0.0 0.0 0.0023531455433910475 0.0 30-31 0.0 0.0 0.0 0.004897603732586245 0.0 32-33 0.0 0.0 0.0 0.008628200325767173 0.0 34-35 0.0 0.0 0.0 0.012970997385521382 0.0 36-37 0.0 0.0 0.0 0.017772944795205553 0.0 38-39 0.0 0.0 0.0 0.02276620485069387 0.0 40-41 0.0 0.0 0.0 0.029385622395517466 0.0 42-43 0.0 0.0 0.0 0.04044349332299735 0.0 44-45 0.0 0.0 0.0 0.054332791408378656 0.0 46-47 0.0 0.0 0.0 0.0836801512747353 0.0 48-49 0.0 0.0 0.0 0.14015564431612043 0.0 50-51 0.0 0.0 0.0 0.18297141444708925 0.0 52-53 0.0 0.0 0.0 0.22850382414847697 0.0 54-55 0.0 0.0 0.0 0.29117784691391657 0.0 56-57 0.0 0.0 0.0 0.34156959781872975 0.0 58-59 0.0 0.0 0.0 0.403076613444764 0.0 60-61 0.0 0.0 0.0 0.48997081716900903 0.0 62-63 0.0 0.0 0.0 0.5514778327950434 0.0 64-65 0.0 0.0 0.0 0.6271419842105848 0.0 66-67 0.0 0.0 0.0 0.7573876334740501 0.0 68-69 0.0 0.0 0.0 0.8987868099878975 0.0 70-71 0.0 0.0 0.0 1.024326168164581 0.0 72-73 0.0 0.0 0.0 1.1730908815418881 0.0 74-75 0.0 0.0 0.0 1.324055690345943 0.0 76-77 0.0 0.0 0.0 1.4497480986392697 0.0 78-79 0.0 0.0 0.0 1.6099724395002455 0.0 80-81 0.0 0.0 0.0 1.833942153943164 0.0 82-83 0.0 0.0 0.0 2.0421477063718205 0.0 84-85 0.0 0.0 0.0 2.272239425480472 0.0 86-87 0.0 0.0 0.0 2.6668217574515323 0.0 88-89 0.0 0.0 0.0 3.17701432128204 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 22320 0.0 28.241245 1 GTACATG 12425 0.0 26.513763 1 GGTATCA 15820 0.0 26.142511 1 TACATGG 12870 0.0 25.442429 2 ACATGGG 13030 0.0 24.179853 3 GAGTACT 14230 0.0 23.56389 12-13 ATCAACG 26775 0.0 23.35667 3 TCAACGC 26800 0.0 23.298973 4 TATCAAC 26940 0.0 23.233042 2 CAACGCA 26915 0.0 23.199425 5 AACGCAG 27320 0.0 22.919807 6 AGTACTT 14705 0.0 22.883476 12-13 CATGGGA 8815 0.0 22.156223 4 GTACTTT 15160 0.0 22.009329 14-15 AGAGTAC 22545 0.0 21.036533 10-11 ACGCAGA 29700 0.0 20.955568 7 CGCAGAG 29715 0.0 20.897024 8 GTGTAGC 1255 0.0 20.075514 1 GCAGAGT 30625 0.0 19.824673 9 CATGGGG 4145 0.0 19.030918 4 >>END_MODULE