FastQCFastQC Report
Thu 26 May 2016
SRR937781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613523
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182890.6997833958224206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154590.5915004382972716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104710.4006469428430513No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80810.30919949814866754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34640.13254140101311523No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30810.11788685234451735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26550.10158701492200374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG700.00839141327.1442369
GTACATG126200.025.0410461
TACATGG129150.024.0546462
GTATCAA256900.024.0289751
GGTATCA193000.023.342171
ACATGGG129850.022.9372443
GAGTACT151050.022.83134712-13
GTACTTT157700.021.77571514-15
AGTACTT154400.021.76681112-13
GTGTAGC11000.021.1686691
CATGGGG40050.020.637754
TCAACGC303200.020.304464
ATCAACG303450.020.2877333
TATCAAC306150.020.2019082
CAACGCA305350.020.1459375
AGAGTAC240200.019.80585710-11
AACGCAG311350.019.785956
CATGGGA86500.019.4952094
ACTTTTT184950.018.68290716-17
TACTTTT181000.018.40835214-15
Produced by FastQC (version 0.11.5)