##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937781_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2613523 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29093755823079 33.0 31.0 34.0 31.0 34.0 2 32.487818932529 34.0 31.0 34.0 31.0 34.0 3 32.546290964342 34.0 31.0 34.0 31.0 34.0 4 36.14039746350042 37.0 35.0 37.0 35.0 37.0 5 36.07323983756791 37.0 35.0 37.0 35.0 37.0 6 36.12638266431939 37.0 35.0 37.0 35.0 37.0 7 36.08106835103422 37.0 35.0 37.0 35.0 37.0 8 36.05590193772927 37.0 35.0 37.0 35.0 37.0 9 37.7491508588216 39.0 37.0 39.0 35.0 39.0 10-11 37.679795241901445 39.0 37.0 39.0 35.0 39.0 12-13 37.583862280913536 39.0 37.0 39.0 35.0 39.0 14-15 38.79817108936864 40.0 38.0 41.0 35.0 41.0 16-17 38.77727898319624 40.0 38.0 41.0 35.0 41.0 18-19 38.79195629806969 40.0 38.0 41.0 35.0 41.0 20-21 38.71125947619363 40.0 38.0 41.0 34.5 41.0 22-23 38.654232811419675 40.0 38.0 41.0 34.0 41.0 24-25 38.66029589179051 40.0 38.0 41.0 34.0 41.0 26-27 38.48177192242043 40.0 38.0 41.0 34.0 41.0 28-29 38.44491936745917 40.0 38.0 41.0 34.0 41.0 30-31 38.42448775082522 40.0 38.0 41.0 34.0 41.0 32-33 38.265684862922576 40.0 38.0 41.0 34.0 41.0 34-35 38.099624529801346 40.0 37.0 41.0 34.0 41.0 36-37 37.919526248668944 40.0 37.0 41.0 33.0 41.0 38-39 37.768706072225115 40.0 37.0 41.0 33.0 41.0 40-41 37.591746083734485 40.0 37.0 41.0 33.0 41.0 42-43 37.34459539862477 40.0 37.0 41.0 33.0 41.0 44-45 36.99357438216538 40.0 36.0 41.0 31.5 41.0 46-47 36.569556112572954 40.0 36.0 41.0 30.5 41.0 48-49 36.16529642172654 39.5 36.0 41.0 30.0 41.0 50-51 35.8986523171979 39.0 36.0 41.0 29.0 41.0 52-53 35.64813146851969 39.0 35.0 41.0 28.0 41.0 54-55 35.433352413581204 39.0 35.0 40.0 27.5 41.0 56-57 35.17621367785935 39.0 35.0 40.0 27.0 41.0 58-59 34.9386376167342 38.0 35.0 40.0 26.0 41.0 60-61 34.60957183081993 38.0 34.0 40.0 26.0 41.0 62-63 34.23492408522902 38.0 34.0 40.0 25.0 41.0 64-65 33.864161708161745 37.0 34.0 40.0 23.5 41.0 66-67 33.460562237256 36.5 33.0 39.0 22.5 41.0 68-69 33.008462714887145 36.0 33.0 39.0 22.0 40.0 70-71 32.59189722072467 35.5 32.0 39.0 20.5 40.0 72-73 32.12733788835989 35.0 32.0 38.0 19.5 40.0 74-75 31.65626225596637 35.0 31.0 37.0 18.0 39.0 76-77 30.35427524456452 34.0 29.5 36.0 16.5 38.5 78-79 30.95085426835731 34.0 31.0 36.0 16.5 39.0 80-81 30.768737447499028 34.0 31.0 36.0 12.0 37.5 82-83 30.38453478312607 34.0 31.0 35.5 7.5 37.0 84-85 29.98427486576548 34.0 30.0 35.0 6.0 37.0 86-87 29.668034870938577 34.0 30.0 35.0 2.0 36.0 88-89 29.281501444601787 34.0 29.5 35.0 2.0 36.0 90-91 28.809040326027358 34.0 29.0 35.0 2.0 35.5 92-93 28.3891410176991 34.0 29.0 35.0 2.0 35.0 94-95 27.93270348108664 33.5 28.5 35.0 2.0 35.0 96-97 27.45477273396867 33.0 27.0 35.0 2.0 35.0 98-99 26.711364506836176 33.0 25.5 35.0 2.0 35.0 100-101 24.352431564596905 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2579232763874373 1101 2 0.2339437702698106 1101 3 0.20812567555854145 1101 4 0.14778556629484996 1101 5 0.14578945334995552 1101 6 0.13726987164391602 1101 7 0.1621299299049781 1101 8 0.17378288677064546 1101 9 0.18205658178545292 1101 10-11 0.2219399461745084 1101 12-13 0.2409323763757456 1101 14-15 0.3131215493568078 1101 16-17 0.31375305129492403 1101 18-19 0.21143286399323102 1101 20-21 0.2358550777718449 1101 22-23 0.20777232908168486 1101 24-25 0.19816740200553085 1101 26-27 0.09077983933839562 1101 28-29 0.25584757257674084 1101 30-31 0.32363750771011013 1101 32-33 0.3016547276219086 1101 34-35 0.24865524891598056 1101 36-37 0.16995921754347165 1101 38-39 0.271174542638299 1101 40-41 0.25335279088274376 1101 42-43 0.2772476307615932 1101 44-45 0.2524763479603891 1101 46-47 0.349864548119605 1101 48-49 0.3092619158711827 1101 50-51 0.23593513803556476 1101 52-53 0.2649556626411069 1101 54-55 0.16048484931584994 1101 56-57 0.25090372105079695 1101 58-59 0.3253089520723975 1101 60-61 0.3535566898041864 1101 62-63 0.3142297253010824 1101 64-65 0.4832683947473768 1101 66-67 0.3060566033258638 1101 68-69 0.3782215184866047 1101 70-71 0.33049841177841444 1101 72-73 0.3434451038695343 1101 74-75 0.3975170048389636 1101 76-77 0.30694023756978694 1101 78-79 0.4512103875266824 1101 80-81 0.5639397068825787 1101 82-83 0.5062819184469021 1101 84-85 0.5118511836719648 1101 86-87 0.5528038428553614 1101 88-89 0.5744700421333846 1101 90-91 0.5832937963843854 1101 92-93 0.7726469094913675 1101 94-95 0.7772293173457356 1101 96-97 0.8127174747976618 1101 98-99 0.789501107913928 1101 100-101 1.1419868389066998 1102 1 0.12031691836302372 1102 2 0.17753446831972752 1102 3 0.10703892208874777 1102 4 0.1248119922007902 1102 5 0.03275491386212792 1102 6 0.0996306817773629 1102 7 0.11020183611164214 1102 8 0.12893548488401052 1102 9 0.20250945478952076 1102 10-11 0.17601475850950976 1102 12-13 0.1688758290034471 1102 14-15 0.10399045558399678 1102 16-17 0.18067652420430136 1102 18-19 0.23395902826505477 1102 20-21 0.2826229073370854 1102 22-23 0.1995629924305362 1102 24-25 0.2057252049119569 1102 26-27 0.24287005110321047 1102 28-29 0.23955120161338783 1102 30-31 0.21471021676597957 1102 32-33 0.1805510433341908 1102 34-35 0.1757858181591132 1102 36-37 0.2055752297120108 1102 38-39 0.251372171291699 1102 40-41 0.2916960362673322 1102 42-43 0.17526324807648308 1102 44-45 0.2333563582996092 1102 46-47 0.08249277091238127 1102 48-49 0.01472072853736961 1102 50-51 0.030352188208659925 1102 52-53 0.12355512253838441 1102 54-55 0.20386020809517902 1102 56-57 0.10816743069575097 1102 58-59 0.14261362640701947 1102 60-61 0.15682830317329888 1102 62-63 0.1352465307777564 1102 64-65 0.2310060308295121 1102 66-67 0.16361365510213233 1102 68-69 0.21945542236924354 1102 70-71 0.036870851012274386 1102 72-73 0.1467506614414873 1102 74-75 0.2263266424238175 1102 76-77 0.06429578004459202 1102 78-79 0.06477163554034249 1102 80-81 0.24494871666022888 1102 82-83 0.27055112670869264 1102 84-85 0.28626798843764334 1102 86-87 0.2758030356484582 1102 88-89 0.3246345740422534 1102 90-91 0.4826033644180292 1102 92-93 0.4273603430128503 1102 94-95 0.656744254687144 1102 96-97 0.7102199153973103 1102 98-99 0.5994582384499125 1102 100-101 1.1177739000417013 1103 1 0.14187203458406117 1103 2 0.1955375863151687 1103 3 0.17668090711298845 1103 4 0.08025579470499622 1103 5 0.08419828414078978 1103 6 0.10574439832697635 1103 7 0.058054322083862075 1103 8 0.029730481304646617 1103 9 0.12156455856986526 1103 10-11 0.12547458321645877 1103 12-13 0.17201195232421895 1103 14-15 0.1922872736788861 1103 16-17 0.1740319451159138 1103 18-19 0.18982723644136712 1103 20-21 0.1401502075696257 1103 22-23 0.15803059648634843 1103 24-25 0.03695392686794463 1103 26-27 0.1449741648524565 1103 28-29 0.13552044961914333 1103 30-31 0.19629975463298877 1103 32-33 0.15117854524828545 1103 34-35 0.17509500373387255 1103 36-37 0.21776249711530937 1103 38-39 0.3109092847501955 1103 40-41 0.2932091811133688 1103 42-43 0.36752825920557797 1103 44-45 0.38496408802783577 1103 46-47 0.39716362902936453 1103 48-49 0.2463170199284761 1103 50-51 0.39255239394232433 1103 52-53 0.4207004079032117 1103 54-55 0.4550045749807339 1103 56-57 0.4254195568117751 1103 58-59 0.33231851873095763 1103 60-61 0.27013857499908056 1103 62-63 0.2478846492317146 1103 64-65 0.38599976427004634 1103 66-67 0.4437180219308132 1103 68-69 0.4654806179904014 1103 70-71 0.500736475787896 1103 72-73 0.5017071794097632 1103 74-75 0.5959431062359464 1103 76-77 0.42353012235442833 1103 78-79 0.492211061760937 1103 80-81 0.4827936173585421 1103 82-83 0.508065637899282 1103 84-85 0.3992000629140833 1103 86-87 0.6215642751120711 1103 88-89 0.7023458473801227 1103 90-91 0.6190673827079962 1103 92-93 0.7352055209610988 1103 94-95 0.7739321156896644 1103 96-97 1.0201028803792234 1103 98-99 1.0752296153086434 1103 100-101 1.5124063057251824 1104 1 0.1579626915937098 1104 2 0.13039571969981978 1104 3 0.11448469352726676 1104 4 0.12406882993037271 1104 5 0.14676965967304056 1104 6 0.07790636137644213 1104 7 0.11479805245711105 1104 8 0.07109451571457726 1104 9 0.14383323867043885 1104 10-11 0.19292379299516682 1104 12-13 0.17238772172208883 1104 14-15 0.14233594268011984 1104 16-17 0.14255794743492345 1104 18-19 0.16737437709502245 1104 20-21 0.21642204058562697 1104 22-23 0.22368175030371873 1104 24-25 0.16990874512336518 1104 26-27 0.07172719652059811 1104 28-29 0.11436644272433227 1104 30-31 0.23130644631692832 1104 32-33 0.23322566111792753 1104 34-35 0.22793427988514026 1104 36-37 0.22016180841128374 1104 38-39 0.3363834855629193 1104 40-41 0.33717201383876017 1104 42-43 0.4008825617591114 1104 44-45 0.3244003391686263 1104 46-47 0.2461037236362671 1104 48-49 0.14024423170739198 1104 50-51 0.17107101937228464 1104 52-53 0.39672697636686394 1104 54-55 0.35457075753298284 1104 56-57 0.3448156238316429 1104 58-59 0.2390889526650355 1104 60-61 0.19288157740264467 1104 62-63 0.19107531411393808 1104 64-65 0.26251219661212133 1104 66-67 0.39997156337211237 1104 68-69 0.44996618932025 1104 70-71 0.428658972772503 1104 72-73 0.2839568924497513 1104 74-75 0.3883432096484647 1104 76-77 0.307125437300936 1104 78-79 0.49355486561149675 1104 80-81 0.5249072467199234 1104 82-83 0.4520930062548594 1104 84-85 0.3687778064802032 1104 86-87 0.35789747043246223 1104 88-89 0.3513107745977351 1104 90-91 0.4508258377016823 1104 92-93 0.5727704818072148 1104 94-95 0.6039354007865043 1104 96-97 0.6959324645228975 1104 98-99 0.778250665198378 1104 100-101 1.1663166157780616 1105 1 0.21674870464100593 1105 2 0.22309222857184352 1105 3 0.1698000303460674 1105 4 0.15024769955586237 1105 5 0.1834711045045907 1105 6 0.1158391184908183 1105 7 0.13301055984600652 1105 8 0.1089384020967259 1105 9 0.1961091199442393 1105 10-11 0.13564195215460728 1105 12-13 0.23690806457695857 1105 14-15 0.2402233565953651 1105 16-17 0.2910258772135279 1105 18-19 0.3274862853651328 1105 20-21 0.32506555445022656 1105 22-23 0.2456277209786677 1105 24-25 0.16760409880338045 1105 26-27 0.2428804963661122 1105 28-29 0.31358778465453696 1105 30-31 0.32106537584919437 1105 32-33 0.35005975412389034 1105 34-35 0.3003535987757715 1105 36-37 0.3207784590888636 1105 38-39 0.3303808859983661 1105 40-41 0.38830296722153435 1105 42-43 0.34288994631769043 1105 44-45 0.4293870837865299 1105 46-47 0.4460666325372671 1105 48-49 0.4061425936808192 1105 50-51 0.4923181881318115 1105 52-53 0.45235278697759895 1105 54-55 0.524218100106836 1105 56-57 0.6453965859612296 1105 58-59 0.7286020150051158 1105 60-61 0.6699316989655699 1105 62-63 0.5196325787416569 1105 64-65 0.538136676074437 1105 66-67 0.7300216522938499 1105 68-69 0.627200249073006 1105 70-71 0.575054442175734 1105 72-73 0.48705807298969717 1105 74-75 0.5411140384235118 1105 76-77 0.6168294837371064 1105 78-79 0.6335163872620377 1105 80-81 0.5969421391289593 1105 82-83 0.6057903474771571 1105 84-85 0.6867542900349086 1105 86-87 0.9173831651771778 1105 88-89 0.8018763979194503 1105 90-91 0.8565199015447824 1105 92-93 0.9244382807150622 1105 94-95 1.1065562586071032 1105 96-97 1.1004870194220615 1105 98-99 1.1295553308990698 1105 100-101 1.4992516883766562 1106 1 -0.21772461004177046 1106 2 -0.14141135914250924 1106 3 -0.180032128736336 1106 4 -0.17166369569149964 1106 5 -0.16326549470090157 1106 6 -0.0945841061166206 1106 7 -0.08927615320698834 1106 8 -0.10681684908094269 1106 9 -0.24885784145023138 1106 10-11 -0.29660383981867966 1106 12-13 -0.44021600213009293 1106 14-15 -0.3326373954618731 1106 16-17 -0.2783768482537141 1106 18-19 -0.20360841588933454 1106 20-21 -0.24941641236006262 1106 22-23 -0.28183972409703273 1106 24-25 -0.3084363807259578 1106 26-27 -0.29632427990954824 1106 28-29 -0.269289287819511 1106 30-31 -0.23372664592893955 1106 32-33 -0.34583961758478665 1106 34-35 -0.6102990625536933 1106 36-37 -0.3759541153238999 1106 38-39 -0.375180812661867 1106 40-41 -0.40860575659907283 1106 42-43 -0.48955840335253953 1106 44-45 -0.45711964561982654 1106 46-47 -0.44555746782741323 1106 48-49 -0.501457815482901 1106 50-51 -0.4916939700373675 1106 52-53 -0.5560712689496086 1106 54-55 -0.6835118085280243 1106 56-57 -0.7797006092525152 1106 58-59 -0.8560389052272583 1106 60-61 -0.8678942915924068 1106 62-63 -0.7724889954183354 1106 64-65 -1.10709643944935 1106 66-67 -1.1490053175832813 1106 68-69 -1.0587037499704586 1106 70-71 -0.9120539819743456 1106 72-73 -1.0024141519392629 1106 74-75 -1.2178571304092536 1106 76-77 -1.1817350539318916 1106 78-79 -0.947317926378993 1106 80-81 -1.0758092609575627 1106 82-83 -1.1339333681549952 1106 84-85 -0.9500552402976723 1106 86-87 -0.8192937117531329 1106 88-89 -0.9818079397055826 1106 90-91 -1.000594307950621 1106 92-93 -1.2115771180006725 1106 94-95 -1.0775245799431623 1106 96-97 -1.2948182363923522 1106 98-99 -1.3476716386956227 1106 100-101 -0.7600485249138238 1107 1 0.11094898828797284 1107 2 0.09953039993880708 1107 3 0.018709657533875657 1107 4 0.021167265134927504 1107 5 0.06085075337217205 1107 6 -0.03365590135113905 1107 7 0.022519362230738693 1107 8 -0.00135805154238966 1107 9 0.07691677867025248 1107 10-11 -0.016800021233812856 1107 12-13 0.06122771811323702 1107 14-15 0.02157294524707254 1107 16-17 0.14483032760776382 1107 18-19 0.20850222986696565 1107 20-21 0.19230755414843514 1107 22-23 0.1680441712989662 1107 24-25 0.04486574055957959 1107 26-27 0.14883893120920533 1107 28-29 0.1988876667334054 1107 30-31 0.17894332855263428 1107 32-33 0.12274517037945998 1107 34-35 0.20166440199363933 1107 36-37 0.1419983301967278 1107 38-39 0.2780579514774857 1107 40-41 0.15607571948699217 1107 42-43 0.2521267863752783 1107 44-45 0.32490844522317985 1107 46-47 0.13484942250051546 1107 48-49 0.21666809718857394 1107 50-51 0.13945607503499247 1107 52-53 0.18044527188063597 1107 54-55 0.1200757710634548 1107 56-57 0.13885460702711327 1107 58-59 0.19777529028496588 1107 60-61 0.19512073604043678 1107 62-63 0.19421539458478065 1107 64-65 0.22467905051508552 1107 66-67 0.3767105103160304 1107 68-69 0.31481371538251324 1107 70-71 0.23821356777957448 1107 72-73 0.265306159619378 1107 74-75 0.4193775584058308 1107 76-77 0.3258301806024164 1107 78-79 0.4236148382951086 1107 80-81 0.48625490784232994 1107 82-83 0.4553976997715914 1107 84-85 0.6000962299342518 1107 86-87 0.6289370286526221 1107 88-89 0.6657464895007088 1107 90-91 0.6463769646250661 1107 92-93 0.5718588258176389 1107 94-95 0.7899511318966965 1107 96-97 0.7694100078522013 1107 98-99 0.7776460876105347 1107 100-101 1.2968434835077112 1108 1 0.13354187452652155 1108 2 0.11785040245017342 1108 3 0.07589974201885497 1108 4 0.10899945126446653 1108 5 0.02362342207113244 1108 6 0.04570242535969271 1108 7 0.10284728761586592 1108 8 0.09099475309523086 1108 9 0.07687626274478987 1108 10-11 0.17379230458598016 1108 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-0.0026272295235933996 2316 94-95 -0.029951444947055705 2316 96-97 0.1364171851067404 2316 98-99 -0.05575633114204237 2316 100-101 0.312736308711429 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 15.0 4 26.0 5 65.0 6 120.0 7 293.0 8 711.0 9 1432.0 10 2453.0 11 4133.0 12 6674.0 13 9430.0 14 11975.0 15 16583.0 16 23172.0 17 34373.0 18 31143.0 19 17375.0 20 13326.0 21 13156.0 22 13173.0 23 14098.0 24 16017.0 25 18664.0 26 22042.0 27 27196.0 28 34415.0 29 42920.0 30 53774.0 31 67821.0 32 86775.0 33 115544.0 34 160698.0 35 236422.0 36 368557.0 37 590790.0 38 512159.0 39 45994.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99520754543645 20.278169067997734 15.333864127023014 21.392759259542803 2 15.134245996687229 21.83615755438158 40.12105498975904 22.90854145917216 3 18.94373992499779 26.03072557616673 31.240436759117866 23.785097739717614 4 12.284835450080218 17.95725539817327 38.220976054161376 31.536933097585134 5 12.550065376283847 38.90392077856132 34.52874164907419 14.017272196080638 6 29.946512810486077 38.20930598276732 17.45697282939542 14.387208377351184 7 26.827925371232624 34.90812975435839 21.109628650675734 17.15431622373325 8 26.032523915037288 35.210288947141464 19.896897788923226 18.86028934889802 9 26.326877551871554 17.089308186689 21.004062332721006 35.57975192871844 10-11 24.200418362493846 27.812037621249168 27.457937045130272 20.52960697112671 12-13 25.65346109577132 25.61230768288875 28.166402131788875 20.56782908955105 14-15 22.330184199641636 26.645795732427075 27.65290376246928 23.371116305462014 16-17 20.642290119505358 29.983015263305507 28.14467291850885 21.230021698680286 18-19 20.52023265148231 27.990187957021995 31.527290940236607 19.962288451259084 20-21 21.62785118455232 26.80629067552216 31.596881446050322 19.96897669387519 22-23 21.111565804197426 26.94653440646003 31.453601504045654 20.488298285296892 24-25 21.053287076486413 27.042826866264424 31.76576215323148 20.13812390401768 26-27 21.023207406932663 27.263006756272006 31.64345862201896 20.070327214776366 28-29 20.459666628531068 27.66530539154484 31.748709862440172 20.126318117483923 30-31 20.82348232634647 27.209900199845187 31.564826481343385 20.40179099246496 32-33 20.715260725706397 27.431062866304 31.424810767843013 20.428865640146583 34-35 21.152807914833733 27.183996467603304 31.412445958960376 20.250749658602587 36-37 21.129596847771957 27.01861648855096 31.394540650499046 20.45724601317803 38-39 20.93605068713763 27.489771469392082 31.31958280068704 20.254595042783247 40-41 20.979055629412994 27.526627418172094 31.191284852202116 20.303032100212796 42-43 20.874245188119108 27.65461193718832 30.69907965428817 20.772063220404398 44-45 21.10607023546378 27.583457272042374 30.47742453385717 20.833047958636676 46-47 21.408707709861364 28.040962333218417 29.79826464125244 20.752065315667778 48-49 21.494473168975365 28.140502302830317 29.56486321337138 20.800161314822944 50-51 21.326672847340543 28.319762251948806 29.530446068391207 20.82311883231944 52-53 21.595084489403764 28.762191876635484 28.821384009247286 20.82133962471346 54-55 21.245460629196682 28.5067321006932 28.966647701206377 21.28115956890374 56-57 21.488465951897116 28.75786821084031 28.787693852321176 20.965971984941397 58-59 21.68743110353343 28.314099397633 28.965423300273237 21.033046198560335 60-61 21.405111032120246 28.441437094680243 29.425798051136336 20.727653822063168 62-63 21.2951636545766 28.438548273728603 29.410703483382395 20.855584588312404 64-65 21.225544982768472 28.49517681688663 29.299742148815984 20.979536051528914 66-67 21.229945173621964 28.503192816745827 28.93932825538555 21.327533754246662 68-69 21.207274625094175 28.524638964340472 29.013289724253433 21.254796686311924 70-71 21.41255309404203 28.60053269093098 28.877840371024092 21.109073844002904 72-73 21.51459925931396 28.35714474293894 28.883962375689826 21.244293622057274 74-75 21.084069281196303 29.097830782434286 28.389017429729908 21.429082506639503 76-77 21.274521020094333 28.67954481364809 28.64342498612027 21.402509180137308 78-79 21.339031644259492 28.76728079301387 28.42242061768731 21.47126694503932 80-81 21.26652415149972 28.98321537633302 28.384961601638857 21.365298870528402 82-83 21.429078408343702 28.860674879994065 28.404067852642218 21.306178859020022 84-85 21.218381208157435 28.71481226255655 28.35931428798818 21.70749224129784 86-87 21.154625384968874 28.664947658773233 28.391083606304594 21.7893433499533 88-89 21.249784294991855 28.508779146003306 28.334531588204886 21.90690497079995 90-91 21.227204168642995 28.381679415603212 28.47891560790127 21.91220080785252 92-93 21.258582986889003 28.449963745310324 28.509386999202206 21.782066268598467 94-95 21.041884842796485 28.336655158573315 28.667855610989456 21.953604387640745 96-97 20.88808860683453 28.429441791788324 28.72620596795972 21.95626363341742 98-99 20.87161443830348 28.582155812507064 28.136451927707085 22.409777821482365 100-101 20.674060386960097 28.522172454957374 28.460389103092858 22.34337805498967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1194.0 1 867.5 2 648.0 3 769.0 4 691.5 5 707.0 6 1244.0 7 2576.0 8 4193.0 9 5151.5 10 5035.5 11 4676.5 12 4866.0 13 5265.5 14 5669.0 15 6068.5 16 6384.5 17 6754.5 18 7054.5 19 7431.5 20 8192.0 21 9263.0 22 10703.0 23 12349.5 24 14381.5 25 17341.0 26 21395.0 27 25614.0 28 28716.0 29 33145.5 30 38691.5 31 43052.0 32 48520.0 33 55266.0 34 62534.0 35 68543.5 36 74576.5 37 80258.0 38 82748.5 39 83937.5 40 85481.0 41 87976.5 42 90698.5 43 93618.0 44 95733.0 45 96275.5 46 94606.0 47 98571.5 48 112770.0 49 109131.5 50 91952.0 51 82228.0 52 75240.0 53 70371.0 54 65616.5 55 60000.0 56 53059.0 57 46372.0 58 41598.0 59 36816.5 60 31354.0 61 25732.0 62 20041.0 63 14851.5 64 10560.5 65 7908.0 66 5601.0 67 4103.0 68 2813.5 69 1626.5 70 1129.5 71 867.0 72 779.5 73 662.0 74 488.0 75 373.5 76 267.5 77 161.0 78 83.5 79 49.0 80 31.0 81 23.0 82 14.0 83 4.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04143831908117893 2 0.0 3 0.0 4 0.0 5 0.007269880540557707 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005012391320068734 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.5305011664332015E-4 22-23 0.0014731073726919563 24-25 0.0 26-27 9.75694493601166E-4 28-29 0.0016070262247548615 30-31 0.0 32-33 1.9131264580415018E-5 34-35 0.0 36-37 2.4870643954539527E-4 38-39 0.0 40-41 1.7218138122373515E-4 42-43 0.020566109423946145 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.4870643954539527E-4 84-85 2.295751749649802E-4 86-87 0.0 88-89 0.0 90-91 0.011421364954507766 92-93 0.002601851982936443 94-95 0.0 96-97 0.0 98-99 6.313317311536956E-4 100-101 0.013755379233318397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2613523.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.822278861085834 #Duplication Level Percentage of deduplicated Percentage of total 1 65.95263203724751 27.58289368684349 2 16.087107394386013 13.455989830324958 3 6.753371874852343 8.473242054080663 4 3.4715144193100893 5.807465764586681 5 2.0665791283822 4.321452429785003 6 1.3038201962678853 3.2717239099818696 7 0.8777932117419248 2.569791873868726 8 0.6264846230675221 2.096081168648972 9 0.4506204969117575 1.6961378474128135 >10 2.175273697377021 15.857614191018191 >50 0.14590322628573696 4.248763292270715 >100 0.08293906276973267 6.100686241051082 >500 0.0033929747970834596 0.942113813572463 >1k 0.002200848517024665 1.5702408251196467 >5k 9.170202154269437E-5 0.3099215032555471 >10k+ 2.751060646280831E-4 1.6958815681793142 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18289 0.6997833958224206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15459 0.5915004382972716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10471 0.4006469428430513 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8081 0.30919949814866754 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3464 0.13254140101311523 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3081 0.11788685234451735 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2655 0.10158701492200374 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.9131264580415018E-5 0.0 16-17 0.0 0.0 0.0 3.8262529160830036E-5 0.0 18-19 0.0 0.0 0.0 3.8262529160830036E-5 0.0 20-21 0.0 0.0 0.0 1.147875874824901E-4 0.0 22-23 0.0 0.0 0.0 3.4436276244747036E-4 0.0 24-25 0.0 0.0 0.0 6.695942603145257E-4 0.0 26-27 0.0 0.0 0.0 0.001147875874824901 0.0 28-29 0.0 0.0 0.0 0.0024105393371322923 0.0 30-31 0.0 0.0 0.0 0.005012391320068735 0.0 32-33 0.0 0.0 0.0 0.008819512971571323 0.0 34-35 0.0 0.0 0.0 0.013257966354227609 0.0 36-37 0.0 0.0 0.0 0.01809817629307261 0.0 38-39 0.0 0.0 0.0 0.023110567613141342 0.0 40-41 0.0 0.0 0.0 0.029729985157964937 0.0 42-43 0.0 0.0 0.0 0.040711331027123154 0.0 44-45 0.0 0.0 0.0 0.05467715417082612 0.0 46-47 0.0 0.0 0.0 0.08412017036008484 0.0 48-49 0.0 0.0 0.0 0.1409974199576587 0.0 50-51 0.0 0.0 0.0 0.18425320917397703 0.0 52-53 0.0 0.0 0.0 0.2300343253149102 3.8262529160830036E-5 54-55 0.0 0.0 0.0 0.2931101046365385 3.8262529160830036E-5 56-57 0.0 0.0 0.0 0.34384621830379913 3.8262529160830036E-5 58-59 0.0 0.0 0.0 0.4056975966922809 3.8262529160830036E-5 60-61 0.0 0.0 0.0 0.4931466070893579 3.8262529160830036E-5 62-63 0.0 0.0 0.0 0.5549405916840984 3.8262529160830036E-5 64-65 0.0 0.0 0.0 0.6307386619517028 3.8262529160830036E-5 66-67 0.0 0.0 0.0 0.761673036740063 3.8262529160830036E-5 68-69 0.0 0.0 0.0 0.9037800700433859 3.8262529160830036E-5 70-71 0.0 0.0 0.0 1.030180335126188 3.8262529160830036E-5 72-73 0.0 0.0 0.0 1.1794998551763272 3.8262529160830036E-5 74-75 0.0 0.0 0.0 1.3312299145635986 3.8262529160830036E-5 76-77 0.0 0.0 0.0 1.4579554111442676 3.8262529160830036E-5 78-79 0.0 0.0 0.0 1.6201502722570262 3.8262529160830036E-5 80-81 0.0 0.0 0.0 1.8479462396160278 3.8262529160830036E-5 82-83 0.0 0.0 0.0 2.058332756206852 3.8262529160830036E-5 84-85 0.0 0.0 0.0 2.2906054394776705 3.8262529160830036E-5 86-87 0.0 0.0 0.0 2.689052286893974 3.8262529160830036E-5 88-89 0.0 0.0 0.0 3.208887008073011 3.8262529160830036E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGCG 70 0.008391413 27.144236 9 GTACATG 12620 0.0 25.041046 1 TACATGG 12915 0.0 24.054646 2 GTATCAA 25690 0.0 24.028975 1 GGTATCA 19300 0.0 23.34217 1 ACATGGG 12985 0.0 22.937244 3 GAGTACT 15105 0.0 22.831347 12-13 GTACTTT 15770 0.0 21.775715 14-15 AGTACTT 15440 0.0 21.766811 12-13 GTGTAGC 1100 0.0 21.168669 1 CATGGGG 4005 0.0 20.63775 4 TCAACGC 30320 0.0 20.30446 4 ATCAACG 30345 0.0 20.287733 3 TATCAAC 30615 0.0 20.201908 2 CAACGCA 30535 0.0 20.145937 5 AGAGTAC 24020 0.0 19.805857 10-11 AACGCAG 31135 0.0 19.78595 6 CATGGGA 8650 0.0 19.495209 4 ACTTTTT 18495 0.0 18.682907 16-17 TACTTTT 18100 0.0 18.408352 14-15 >>END_MODULE