FastQCFastQC Report
Thu 26 May 2016
SRR937780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166296
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162710.751097726257169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123800.571482382832263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84690.39094380454009975No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43390.20029580445146922No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37840.1746760368850794No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29070.1341921879558472No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28310.1306838954602695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27840.12851429352221488No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22730.10492564266379109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA181400.030.724191
GGTATCA122350.029.0339891
GTACATG105100.026.152621
ATCAACG218250.025.4147283
TCAACGC220300.025.2015574
CAACGCA221050.025.0933865
TACATGG106300.025.0840972
TATCAAC221900.025.0181052
AACGCAG223900.024.7831556
ACATGGG107100.024.186663
AGTACTT130600.023.51298912-13
GAGTACT127150.023.04895612-13
ACGCAGA246200.022.421567
CGCAGAG247650.022.1176368
AGAGTAC194450.021.9850310-11
GTACTTT135250.021.58052614-15
CATGGGG32200.021.5524184
GCAGAGT251050.021.4197259
CATGGGA74350.019.9467724
TACTTTT157300.019.37070314-15
Produced by FastQC (version 0.11.5)