##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937780_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2166296 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.939958805260222 33.0 31.0 34.0 30.0 34.0 2 32.03521125460232 33.0 31.0 34.0 30.0 34.0 3 32.06779359792014 34.0 31.0 34.0 30.0 34.0 4 35.67660975231455 37.0 35.0 37.0 35.0 37.0 5 35.69144152045704 37.0 35.0 37.0 35.0 37.0 6 35.76232333900815 37.0 35.0 37.0 35.0 37.0 7 35.69056906350748 37.0 35.0 37.0 33.0 37.0 8 35.702728066709255 37.0 35.0 37.0 33.0 37.0 9 37.29536176035039 39.0 37.0 39.0 34.0 39.0 10-11 37.23266234161906 39.0 37.0 39.0 34.0 39.0 12-13 37.14926238150281 39.0 37.0 39.0 34.0 39.0 14-15 38.29133807199016 40.0 38.0 41.0 33.5 41.0 16-17 38.218976538755555 40.0 38.0 41.0 33.0 41.0 18-19 38.20291986875293 40.0 38.0 41.0 33.5 41.0 20-21 38.23955475152057 40.0 38.0 41.0 34.0 41.0 22-23 38.23282275367724 40.0 38.0 41.0 34.0 41.0 24-25 38.161388840675514 40.0 38.0 41.0 34.0 41.0 26-27 38.05750968473376 40.0 38.0 41.0 33.5 41.0 28-29 37.91842550602503 40.0 38.0 41.0 33.0 41.0 30-31 37.66421878635237 40.0 37.0 41.0 33.0 41.0 32-33 37.48584127007574 40.0 37.0 41.0 33.0 41.0 34-35 37.44210971169222 40.0 37.0 41.0 33.0 41.0 36-37 37.28038389029015 40.0 37.0 41.0 32.5 41.0 38-39 37.111139936555304 40.0 36.0 41.0 32.0 41.0 40-41 36.88046416556186 40.0 36.0 41.0 31.0 41.0 42-43 36.59798891748865 39.0 36.0 41.0 30.5 41.0 44-45 36.28429655965759 39.0 35.5 41.0 30.0 41.0 46-47 35.82301033653758 39.0 35.0 41.0 28.5 41.0 48-49 35.40133181245776 39.0 35.0 40.5 26.5 41.0 50-51 34.50464248653 38.0 34.0 39.5 25.0 40.5 52-53 34.604315153607814 38.0 34.5 40.0 24.5 40.5 54-55 34.89090918323258 39.0 35.0 40.0 25.0 41.0 56-57 34.67470811929672 38.5 34.5 40.0 23.5 41.0 58-59 34.429841535967384 38.0 34.0 40.0 23.0 41.0 60-61 34.11238261068663 38.0 34.0 40.0 22.0 41.0 62-63 33.778795233892325 38.0 34.0 40.0 21.5 41.0 64-65 33.374496836997345 37.0 33.0 40.0 19.5 41.0 66-67 32.878652317134865 36.5 32.5 39.0 16.5 41.0 68-69 32.45312228799757 36.0 32.0 39.0 13.5 40.5 70-71 32.00165720658673 35.5 31.5 39.0 9.5 40.0 72-73 31.54237925011171 35.0 31.0 38.0 7.5 40.0 74-75 31.02081756140435 35.0 31.0 37.0 4.5 39.5 76-77 30.501888938538407 35.0 30.0 37.0 2.0 39.0 78-79 29.991785056151144 34.0 29.5 36.0 2.0 39.0 80-81 29.46357538397338 34.0 29.0 36.0 2.0 37.5 82-83 28.940116678422527 34.0 29.0 35.0 2.0 37.0 84-85 28.414171470565428 34.0 28.5 35.0 2.0 36.5 86-87 27.868021960064553 33.5 26.5 35.0 2.0 36.0 88-89 27.347394585042856 33.0 26.0 35.0 2.0 36.0 90-91 26.80149688685203 33.0 25.5 35.0 2.0 35.0 92-93 26.182264796685217 32.0 24.5 35.0 2.0 35.0 94-95 25.469358757990598 32.0 21.5 34.0 2.0 35.0 96-97 24.652506628826345 31.5 15.0 34.0 2.0 35.0 98-99 23.541361845287994 31.0 2.0 34.0 2.0 35.0 100-101 20.366731277720127 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1762208667519083 1101 2 0.14370053579001052 1101 3 0.10543292031481144 1101 4 0.2007047800767836 1101 5 0.08100105947195146 1101 6 0.12703752473997554 1101 7 0.14957435959397003 1101 8 0.12117032276070461 1101 9 0.3777419114381857 1101 10-11 0.30318683577296923 1101 12-13 0.3299296298301968 1101 14-15 0.4998723177045292 1101 16-17 0.47454512277025884 1101 18-19 0.47489575009702634 1101 20-21 0.5280539175543524 1101 22-23 0.45424628845602655 1101 24-25 0.48080617772356504 1101 26-27 0.48600544603243634 1101 28-29 0.451390904434561 1101 30-31 0.44983343518116925 1101 32-33 0.5425262084807727 1101 34-35 0.5792524643899171 1101 36-37 0.6662451217916256 1101 38-39 0.5238263005112245 1101 40-41 0.6411734109768545 1101 42-43 0.5009818772791448 1101 44-45 0.5089008839331441 1101 46-47 0.598328652887588 1101 48-49 0.8442592901862227 1101 50-51 0.6381371422877038 1101 52-53 0.7449954928345122 1101 54-55 0.8683003090134136 1101 56-57 1.0469948718510622 1101 58-59 1.0446271495839952 1101 60-61 1.0509672509193706 1101 62-63 1.0578962162293024 1101 64-65 1.0519696162090213 1101 66-67 1.1809473802637456 1101 68-69 1.279939390548158 1101 70-71 1.2882631435626237 1101 72-73 1.3309035226199164 1101 74-75 1.3860521735555942 1101 76-77 1.3001980754785158 1101 78-79 1.4018680962326577 1101 80-81 1.7618221551563558 1101 82-83 1.9166226995253055 1101 84-85 1.9959632138932868 1101 86-87 2.071464202372372 1101 88-89 2.0650247977191754 1101 90-91 2.184300060802588 1101 92-93 2.1111868792759587 1101 94-95 2.4817929960421203 1101 96-97 2.676559664064545 1101 98-99 2.941666862197234 1101 100-101 3.495922601606047 1102 1 0.12229709083878859 1102 2 0.1503852377469812 1102 3 0.03578955969931741 1102 4 0.06963612723594537 1102 5 0.09264358663148187 1102 6 0.08586094897869145 1102 7 0.13943727946804785 1102 8 0.18463208074764736 1102 9 0.26267093702363553 1102 10-11 0.28491627065957204 1102 12-13 0.28915822919781675 1102 14-15 0.46823049196834887 1102 16-17 0.39226465796039633 1102 18-19 0.32495509601289285 1102 20-21 0.3891459649265556 1102 22-23 0.36799287933229863 1102 24-25 0.3050116094908901 1102 26-27 0.4434994667423027 1102 28-29 0.43740782386578303 1102 30-31 0.5280036967570609 1102 32-33 0.4812145193295905 1102 34-35 0.5489227129000867 1102 36-37 0.5609881601649889 1102 38-39 0.5912847961919709 1102 40-41 0.5850554178177916 1102 42-43 0.6274410785092499 1102 44-45 0.7644507241874123 1102 46-47 0.8623560194005435 1102 48-49 0.7761776280054562 1102 50-51 0.6050874498824754 1102 52-53 0.6167548138567227 1102 54-55 0.7736058201378526 1102 56-57 0.7488461480977051 1102 58-59 0.6779418012172798 1102 60-61 0.760608727319223 1102 62-63 0.7562762721506218 1102 64-65 0.6932547750539015 1102 66-67 0.9007504178394043 1102 68-69 0.7548768469670861 1102 70-71 0.7399640263932952 1102 72-73 0.7746958573006175 1102 74-75 0.8004481311566245 1102 76-77 0.7690755093143515 1102 78-79 0.9457959706586259 1102 80-81 1.192233670602107 1102 82-83 1.3225708382090815 1102 84-85 1.2951852528896701 1102 86-87 1.3489621290475036 1102 88-89 1.3268027270899694 1102 90-91 1.5848291540547557 1102 92-93 1.6162325199299055 1102 94-95 1.7431703576726143 1102 96-97 1.8369154919985213 1102 98-99 2.3973140077480757 1102 100-101 2.980703228018349 1103 1 -0.044494001782563686 1103 2 -0.06696363458344479 1103 3 0.026614332811917052 1103 4 -0.03987533467403637 1103 5 -0.001070528115846514 1103 6 -0.01803994717312918 1103 7 -0.08501954425076974 1103 8 -0.01672853131407237 1103 9 -0.0027766402320636985 1103 10-11 0.08707401617524368 1103 12-13 0.11981402996432422 1103 14-15 0.22575090114927576 1103 16-17 0.18813936767274697 1103 18-19 0.14448023527972254 1103 20-21 0.15746863657026466 1103 22-23 0.016843838884362583 1103 24-25 0.029068797939224567 1103 26-27 -9.989589369325813E-4 1103 28-29 0.01600774693064011 1103 30-31 0.07355356924419709 1103 32-33 0.2740274957219029 1103 34-35 0.16932120839083353 1103 36-37 0.25430335200416465 1103 38-39 0.19567579763094045 1103 40-41 0.35146824603873483 1103 42-43 0.24406049180763034 1103 44-45 0.24621353602402252 1103 46-47 0.2795410483936891 1103 48-49 0.2556295364241521 1103 50-51 0.27466672868684583 1103 52-53 0.39583201968668646 1103 54-55 0.34392381868463673 1103 56-57 0.4596063811826596 1103 58-59 0.39622458057016985 1103 60-61 0.486939776344002 1103 62-63 0.7298006135716477 1103 64-65 0.45838891288175887 1103 66-67 0.8246345022400945 1103 68-69 0.854928849417611 1103 70-71 0.783076907329054 1103 72-73 0.6151594627088706 1103 74-75 0.6669443057708797 1103 76-77 0.7027766590671618 1103 78-79 0.7460602570852721 1103 80-81 0.8856371953709434 1103 82-83 1.0766740452643155 1103 84-85 1.2001183620663234 1103 86-87 1.3164620221931536 1103 88-89 1.4122163958984721 1103 90-91 1.2284463875243894 1103 92-93 1.2972922301409078 1103 94-95 1.6011060740954157 1103 96-97 1.7529863862982005 1103 98-99 2.1176035118014163 1103 100-101 2.49757766703301 1104 1 0.1305973798807507 1104 2 0.13198093292240287 1104 3 0.15827387868105092 1104 4 0.1511833210382605 1104 5 0.15276955911678414 1104 6 0.17914350880251106 1104 7 0.22528995327009937 1104 8 0.14793882571562733 1104 9 0.37943668194573377 1104 10-11 0.2538052615032953 1104 12-13 0.1890343540691859 1104 14-15 0.3185038037600805 1104 16-17 0.3301550448571575 1104 18-19 0.3502885429530451 1104 20-21 0.23178850325247424 1104 22-23 0.19198631198420912 1104 24-25 0.1937035798919453 1104 26-27 0.21622399860574149 1104 28-29 0.16438310782821475 1104 30-31 0.1967062194838789 1104 32-33 0.17703160361055836 1104 34-35 0.37581770742729503 1104 36-37 0.2924799706425958 1104 38-39 0.34911744023776947 1104 40-41 0.34093620930776325 1104 42-43 0.4055800677144248 1104 44-45 0.4613486025800526 1104 46-47 0.5616648748336814 1104 48-49 0.6871028900111895 1104 50-51 0.5889030660137706 1104 52-53 0.658221521596623 1104 54-55 0.7127103799223491 1104 56-57 0.8140067597393426 1104 58-59 0.9308295991893942 1104 60-61 0.8685180559684582 1104 62-63 0.8892693312108761 1104 64-65 0.963863926083242 1104 66-67 1.1247726891456011 1104 68-69 1.0100969711043817 1104 70-71 1.0288123154090414 1104 72-73 1.0300863916677763 1104 74-75 1.0354136886274752 1104 76-77 1.1889466529636188 1104 78-79 1.2468043404226066 1104 80-81 1.461220975039982 1104 82-83 1.401239082262748 1104 84-85 1.5624615730773854 1104 86-87 1.775859417558575 1104 88-89 1.708616678444404 1104 90-91 1.8080849070385732 1104 92-93 1.9817470605104823 1104 94-95 2.10463241190239 1104 96-97 2.0252207622625953 1104 98-99 2.204025876820584 1104 100-101 3.074578291102906 1105 1 0.05037182648057481 1105 2 0.05702942894896523 1105 3 -0.021328811868272624 1105 4 0.004547700919822262 1105 5 -0.05678236828066474 1105 6 -0.06292615501519094 1105 7 -0.17099468857524158 1105 8 -0.057098624467279535 1105 9 0.0670104487488814 1105 10-11 0.08667346481500005 1105 12-13 0.005798808271556766 1105 14-15 -0.0565370215417218 1105 16-17 -0.019121691027855547 1105 18-19 0.022915305941289432 1105 20-21 -0.01585931161373111 1105 22-23 0.08102876688887051 1105 24-25 0.0028358334145082154 1105 26-27 -0.017045468841409672 1105 28-29 0.04549403332671886 1105 30-31 0.058561239376629715 1105 32-33 -0.024054230094947116 1105 34-35 0.15498435988891401 1105 36-37 0.16304211544542113 1105 38-39 0.1598840695167496 1105 40-41 0.2519251191443601 1105 42-43 0.159125817621522 1105 44-45 0.18104668285333503 1105 46-47 0.18440806237778418 1105 48-49 0.1094772009244096 1105 50-51 0.13524447122236438 1105 52-53 0.0646516344756023 1105 54-55 0.15131048969893612 1105 56-57 0.2517532030799643 1105 58-59 0.28547379688271945 1105 60-61 0.3390099710245522 1105 62-63 0.27436630621794933 1105 64-65 0.39775966559224685 1105 66-67 0.6312215526851119 1105 68-69 0.540310762726584 1105 70-71 0.47255215169873566 1105 72-73 0.5443986003933077 1105 74-75 0.5570976343119227 1105 76-77 0.4867579394975792 1105 78-79 0.7180268493973578 1105 80-81 0.9332320345204899 1105 82-83 0.8964859602777651 1105 84-85 0.9888962542991102 1105 86-87 1.1072285907908146 1105 88-89 1.1305239274497652 1105 90-91 1.076781190657659 1105 92-93 1.3020584578437955 1105 94-95 1.2853908249828514 1105 96-97 1.3447545185653738 1105 98-99 1.5079113546044631 1105 100-101 2.2936558949730426 1106 1 -0.014028640259933667 1106 2 -0.015586769990719063 1106 3 0.02442359581941389 1106 4 -0.029002866863244492 1106 5 -0.1400164910706181 1106 6 0.0501947916563239 1106 7 0.08271556026847549 1106 8 -0.008211360902627973 1106 9 0.05557086712425274 1106 10-11 -0.1717688732843854 1106 12-13 -0.19979055731648288 1106 14-15 -0.40504315596636786 1106 16-17 -0.26840414090821696 1106 18-19 0.04443324839353835 1106 20-21 0.08246651046681563 1106 22-23 0.04723499939667164 1106 24-25 -0.20711918362320603 1106 26-27 -0.037320672406224276 1106 28-29 0.10290882358943065 1106 30-31 0.09180191513571856 1106 32-33 0.23187409310594376 1106 34-35 0.07539527333053542 1106 36-37 -0.04247368777948424 1106 38-39 -0.3095265648952079 1106 40-41 0.017706148510121977 1106 42-43 0.03312934354168107 1106 44-45 -0.127025412490255 1106 46-47 0.02248472366115095 1106 48-49 -0.010832669960961994 1106 50-51 -0.07728205773331354 1106 52-53 0.04010791137447711 1106 54-55 0.10730402551197216 1106 56-57 -0.13251449205055366 1106 58-59 -0.34733332598333533 1106 60-61 0.021041412593206132 1106 62-63 -0.3158663876019645 1106 64-65 -0.043118707072991924 1106 66-67 0.17225666814420038 1106 68-69 0.06788808776230582 1106 70-71 -0.14753069051422685 1106 72-73 -0.23977798732769529 1106 74-75 -0.06853483290209184 1106 76-77 0.3076248127589274 1106 78-79 0.2842180734919886 1106 80-81 0.23040421695514723 1106 82-83 0.11583814643282153 1106 84-85 0.12850673313090866 1106 86-87 0.46004097717078807 1106 88-89 0.6766647570979352 1106 90-91 0.9182770221237462 1106 92-93 1.0702730696966363 1106 94-95 1.1703860053797506 1106 96-97 1.4062414506766139 1106 98-99 1.4198063675835684 1106 100-101 1.9796573789034717 1107 1 0.0679008106122474 1107 2 0.235310539119304 1107 3 0.17803922709395437 1107 4 0.12913763433893166 1107 5 0.1488622932455428 1107 6 0.22408459495141386 1107 7 0.11321542541276841 1107 8 0.17903002158733727 1107 9 0.1402530456547666 1107 10-11 0.1567580644307256 1107 12-13 0.11503656804399753 1107 14-15 0.3275463386589976 1107 16-17 0.11232650614559248 1107 18-19 0.44076278156227744 1107 20-21 0.4258310335040889 1107 22-23 0.35111979458888953 1107 24-25 0.3332458949734658 1107 26-27 0.2293162856640265 1107 28-29 0.18535378056239438 1107 30-31 0.22885997455060902 1107 32-33 0.34908300649170343 1107 34-35 0.37823263158797005 1107 36-37 0.3094548928699865 1107 38-39 0.41992940759883624 1107 40-41 0.46838382724003225 1107 42-43 0.5631788252116792 1107 44-45 0.5257636512884218 1107 46-47 0.5993474324857857 1107 48-49 0.6397223419455287 1107 50-51 0.3717151114495181 1107 52-53 0.4472434782410062 1107 54-55 0.4855597246062544 1107 56-57 0.6405644192835567 1107 58-59 0.5122173394200686 1107 60-61 0.7284363749834597 1107 62-63 0.9139075619643009 1107 64-65 0.820056781692486 1107 66-67 0.9563930268557641 1107 68-69 0.9359823518996819 1107 70-71 1.0294796680175295 1107 72-73 0.9961104293741236 1107 74-75 0.9615106146228989 1107 76-77 1.0135853484472825 1107 78-79 1.1144311397418711 1107 80-81 1.2651936749274277 1107 82-83 1.4648576763050905 1107 84-85 1.6005058767395504 1107 86-87 1.667972480846469 1107 88-89 1.4802167353273852 1107 90-91 1.77450052643567 1107 92-93 1.9954614354529738 1107 94-95 2.205957078946419 1107 96-97 2.268335644504603 1107 98-99 2.4574234730499533 1107 100-101 2.973116156857216 1108 1 0.022916114942621135 1108 2 0.08413099459338014 1108 3 0.08728132224602803 1108 4 0.03811450221990498 1108 5 0.04545233073577748 1108 6 0.1369502680910628 1108 7 0.057549955852977064 1108 8 0.023789632491762802 1108 9 0.1900440895749682 1108 10-11 0.25783474378528837 1108 12-13 0.2730869325522889 1108 14-15 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-1.0718208386405337 2316 52-53 -1.1642107936220611 2316 54-55 -1.343941940876313 2316 56-57 -1.4117975710922153 2316 58-59 -1.4545503223042715 2316 60-61 -1.3177147448735127 2316 62-63 -1.5201264296679966 2316 64-65 -1.5971215742772458 2316 66-67 -1.5994289191017081 2316 68-69 -1.5561146424190397 2316 70-71 -1.4395123671264756 2316 72-73 -1.5679671097645134 2316 74-75 -1.7294862171759746 2316 76-77 -1.5760046327778916 2316 78-79 -1.6801324208181363 2316 80-81 -1.7645888805160972 2316 82-83 -1.9872964237605828 2316 84-85 -2.0665689206396216 2316 86-87 -2.26302331740105 2316 88-89 -2.3327405806292667 2316 90-91 -2.4504006554750646 2316 92-93 -2.4463311088167963 2316 94-95 -2.2507890002724444 2316 96-97 -2.1438515536190543 2316 98-99 -2.3152123189148774 2316 100-101 -2.6105640672362505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6995.0 3 1105.0 4 1077.0 5 1189.0 6 1364.0 7 1769.0 8 2224.0 9 2736.0 10 3771.0 11 5116.0 12 7730.0 13 10603.0 14 12084.0 15 16143.0 16 21606.0 17 30230.0 18 25641.0 19 15029.0 20 13856.0 21 14514.0 22 15803.0 23 17044.0 24 19339.0 25 21868.0 26 25471.0 27 29695.0 28 35187.0 29 42226.0 30 52326.0 31 65232.0 32 83920.0 33 110126.0 34 150140.0 35 212583.0 36 310462.0 37 430393.0 38 319932.0 39 29765.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8302283759582 19.31546478054165 15.197622712027009 21.656684131473135 2 14.71359746756943 20.764218311065036 41.589618515621744 22.932565705743784 3 18.433617169775168 25.68625690422348 31.61252669355384 24.267599232447512 4 11.700091355983817 17.19616730640538 39.29806764045173 31.805673697159083 5 12.379961550672308 38.0621964618103 35.308219399972046 14.249622587545344 6 29.559077916431555 37.49091144556581 18.388851373227386 14.561159264775261 7 26.412369217364525 34.43638655839162 21.835237118751536 17.316007105492325 8 25.392830945097554 34.58674471330533 20.817473172238806 19.202951169358307 9 26.168624568878396 16.741925532342965 21.74878693932222 35.34066295945642 10-11 23.81575149722202 27.136397527479133 28.352722751669283 20.69512822362956 12-13 25.30798812544694 25.14061483838082 28.85633881515347 20.695058221018773 14-15 22.216453504184003 25.933699776692798 28.450438566467657 23.399408152655543 16-17 20.371269648127 29.345642852815313 28.90919509450664 21.373892404551047 18-19 20.183462903344033 27.382454553379443 32.354349642251734 20.07973290102479 20-21 21.235778441036892 26.2037628804478 32.35747456744541 20.202984111069902 22-23 20.780677019331584 26.321805483548566 32.31698264694551 20.580534850174335 24-25 20.655626178891605 26.380856823679554 32.57737818438032 20.386138813048515 26-27 20.54895447648899 26.56672431770928 32.470718334765934 20.413602871035796 28-29 20.140460520686336 26.902851994259102 32.615212270314345 20.341475214740214 30-31 20.36053208165221 26.556279628251446 32.485445182907114 20.597743107189224 32-33 20.274488917832553 26.693244781757354 32.3273765763074 20.7048897241027 34-35 20.771736795158414 26.618561950990728 32.09198621936574 20.517715034485118 36-37 20.659642693541 26.450616634482 32.23002860662321 20.659712065353798 38-39 20.49626536175341 26.798801234651542 32.194447675178154 20.510485728416896 40-41 20.5841659017288 26.86099556759931 32.08572545011873 20.46911308055316 42-43 20.43949315292125 26.88861767675203 31.610861352378407 21.061027817948315 44-45 20.630356525133237 26.93186379789078 31.41952887552051 21.018250801455476 46-47 20.90719657421173 27.26050480797581 30.815074633872115 21.017223983940344 48-49 20.983591998867713 27.374775323150757 30.51490892204141 21.12672375594012 50-51 20.807914490937094 27.601486510862074 30.54835322902165 21.042245769179182 52-53 21.031441327592617 27.901611014178478 29.96360092290556 21.103346735323342 54-55 20.83360505348609 27.506473877171388 30.1351755728439 21.524745496498625 56-57 21.034948038964536 27.91091852239591 29.824903749314473 21.229229689325074 58-59 21.20405243831507 27.49754258971744 30.06189529906429 21.236509672903203 60-61 21.086373819117423 27.466047205476745 30.48705344147546 20.960525533930365 62-63 20.871245294046386 27.55855450120822 30.496660171656913 21.073540033088484 64-65 20.750623689327572 27.643390742808787 30.440755872159293 21.165229695704348 66-67 20.84894754365197 27.663972329091447 29.96811649156555 21.518963635691033 68-69 20.67791233667371 27.625514980829614 30.20902574493759 21.487546937559088 70-71 20.988855188665504 27.64277136373999 29.952472357621694 21.415901089972813 72-73 21.039462475686264 27.379693834230967 30.097953252884118 21.48289043719865 74-75 20.640812071746755 27.9923386141984 29.75189985348744 21.614949460567402 76-77 20.777942390104702 27.73930957011672 29.759501313288812 21.723246726489773 78-79 20.842795342437057 27.733550440244997 29.690957930294715 21.732696287023227 80-81 20.76218337739717 27.95248134821006 29.610991156556647 21.67434411783612 82-83 20.91717639921353 27.813686665692515 29.735957924175473 21.53317901091848 84-85 20.865578755279124 27.615621700366518 29.55123713063468 21.967562413719676 86-87 20.85500703562017 27.699118686264978 29.640769511418608 21.805104766696246 88-89 20.91600607091427 27.42344072118466 29.586250174571155 22.07430303332991 90-91 20.849380498685438 27.27317918960206 29.85645585641285 22.020984455299654 92-93 21.057746062705395 27.22484485031599 29.904595833974923 21.81281325300369 94-95 20.686289878434675 27.202929331744464 29.953096843019395 22.15768394680147 96-97 20.58002212801182 27.28554962212431 30.047828414263204 22.086599835600666 98-99 20.62228603931688 27.50519091947485 29.47852647253322 22.393996568675057 100-101 20.78945646668086 26.74283896476141 30.26556500272688 22.202139565830848 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3055.0 1 1822.5 2 689.5 3 804.0 4 686.0 5 641.5 6 1087.5 7 2391.5 8 4188.0 9 5106.5 10 4617.5 11 3977.0 12 4096.0 13 4382.0 14 4643.0 15 4875.5 16 5089.0 17 5420.0 18 5710.0 19 6091.5 20 6689.0 21 7624.5 22 8844.0 23 10290.5 24 12188.5 25 14809.5 26 18377.0 27 22009.0 28 24723.5 29 28401.5 30 32888.5 31 36644.0 32 41476.0 33 47697.5 34 53426.0 35 57917.5 36 63102.5 37 67448.0 38 69051.0 39 69660.0 40 70834.0 41 73314.0 42 75978.0 43 78139.5 44 79041.0 45 79239.5 46 78782.5 47 81766.0 48 92110.5 49 89917.0 50 75470.5 51 66466.0 52 60624.0 53 56750.5 54 53383.5 55 48742.0 56 43364.5 57 37876.5 58 33136.5 59 28746.5 60 24362.5 61 20126.0 62 15810.5 63 11620.0 64 8259.0 65 6172.5 66 4411.5 67 3183.5 68 2217.0 69 1353.0 70 954.5 71 735.5 72 577.5 73 475.0 74 380.0 75 273.5 76 201.0 77 128.0 78 74.5 79 50.5 80 28.5 81 20.5 82 14.5 83 10.0 84 9.0 85 9.0 86 7.0 87 6.5 88 6.0 89 4.5 90 2.5 91 2.5 92 3.0 93 3.0 94 1.5 95 1.0 96 2.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30115921369932824 2 0.2550897938231894 3 0.261367790920539 4 0.25472049987628653 5 0.2552744407966409 6 0.1955411448850942 7 0.1858010170355298 8 0.1924944698231451 9 0.19401780735411966 10-11 0.19812620251341462 12-13 0.20135752454881511 14-15 0.1962797327789 16-17 0.18773981025677008 18-19 0.18240812889835922 20-21 0.18049241654880036 22-23 0.1884783981505759 24-25 0.1866088475443799 26-27 0.1915481540842064 28-29 0.19687983544261728 30-31 0.19835701123022895 32-33 0.19579503447358995 34-35 0.1974106954912902 36-37 0.186239553597477 38-39 0.1802385269603046 40-41 0.17592240395587674 42-43 0.1840468707877409 44-45 0.18372373858420088 46-47 0.1896093608629661 48-49 0.19858781994704322 50-51 0.19323305771695096 52-53 0.20440419961076417 54-55 0.19134042623907352 56-57 0.17144471484967888 58-59 0.15925801460188266 60-61 0.1740990150930436 62-63 0.166643893539941 64-65 0.17361431678773354 66-67 0.17246027320366192 68-69 0.1715139574647232 70-71 0.173798963761185 72-73 0.18178494536296055 74-75 0.1857548552921669 76-77 0.18356217248243084 78-79 0.18097711485411042 80-81 0.1801692843452603 82-83 0.17190633228330754 84-85 0.168513444146137 86-87 0.17213714100012187 88-89 0.17790735892047993 90-91 0.17497608821693805 92-93 0.17112158264613886 94-95 0.17333734632755635 96-97 0.17903832163287012 98-99 0.1784612998408343 100-101 0.16468201944701924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2166296.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.06085585481436 #Duplication Level Percentage of deduplicated Percentage of total 1 69.16046591664607 31.855902514384365 2 15.222837796569234 14.023538748979906 3 6.172400244666659 8.529181138434357 4 3.110010429092861 5.729989683256625 5 1.7743811895195905 4.086475810097795 6 1.1012492415340505 3.0434689544714124 7 0.7189440199520616 2.3180623809484886 8 0.49899728456710607 1.8387393597111403 9 0.3524198907818038 1.4609485610703077 >10 1.6940027649614018 13.926222104811655 >50 0.12899621321520463 4.138445811167567 >100 0.06029020836687732 4.80071886064065 >500 0.003102976078808905 1.0535931841240862 >1k 0.0016015360406755527 1.4832924872938884 >5k 1.0009600254222205E-4 0.3904638839640663 >10k+ 2.001920050844441E-4 1.3209565166435782 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16271 0.751097726257169 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12380 0.571482382832263 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8469 0.39094380454009975 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4339 0.20029580445146922 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3784 0.1746760368850794 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2907 0.1341921879558472 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2831 0.1306838954602695 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2784 0.12851429352221488 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2273 0.10492564266379109 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.232348672572907E-5 0.0 18-19 0.0 0.0 0.0 9.232348672572907E-5 0.0 20-21 0.0 0.0 0.0 9.232348672572907E-5 0.0 22-23 0.0 0.0 0.0 4.1545569026578085E-4 0.0 24-25 0.0 0.0 0.0 6.924261504429681E-4 0.0 26-27 0.0 0.0 0.0 0.00101555835398302 0.0 28-29 0.0 0.0 0.0 0.0020080358362846076 0.0 30-31 0.0 0.0 0.0 0.004616174336286454 0.0 32-33 0.0 0.0 0.0 0.007455121553102623 0.0 34-35 0.0 0.0 0.0 0.011840487172574753 0.0 36-37 0.0 0.0 0.0 0.016802874584082694 0.0 38-39 0.0 0.0 0.0 0.02128056369028055 0.0 40-41 0.0 0.0 0.0 0.027189266840727214 0.0 42-43 0.0 0.0 0.0 0.03649085812834442 0.0 44-45 0.0 0.0 0.0 0.049854682831893704 0.0 46-47 0.0 0.0 0.0 0.08205249882749172 0.0 48-49 0.0 0.0 0.0 0.14210892694257848 0.0 50-51 0.0 0.0 0.0 0.18963244173464752 0.0 52-53 0.0 0.0 0.0 0.23810227226565528 0.0 54-55 0.0 0.0 0.0 0.30605235849579193 0.0 56-57 0.0 0.0 0.0 0.35678411445158004 0.0 58-59 0.0 0.0 0.0 0.4163327633896753 0.0 60-61 0.0 0.0 0.0 0.5036246200888521 0.0 62-63 0.0 0.0 0.0 0.5644196360977447 0.0 64-65 0.0 0.0 0.0 0.6397325203942583 0.0 66-67 0.0 0.0 0.0 0.7786562870447991 0.0 68-69 0.0 0.0 0.0 0.9336212595139353 0.0 70-71 0.0 0.0 0.0 1.062020148677743 0.0 72-73 0.0 0.0 0.0 1.2275099986336122 0.0 74-75 0.0 0.0 0.0 1.3904147909611613 0.0 76-77 0.0 0.0 0.0 1.5250455154789557 0.0 78-79 0.0 0.0 0.0 1.6941821431604915 0.0 80-81 0.0 0.0 0.0 1.9347771495677413 0.0 82-83 0.0 0.0 0.0 2.147744352572317 0.0 84-85 0.0 0.0 0.0 2.3793839807671713 0.0 86-87 0.0 0.0 0.0 2.8125426996126106 0.0 88-89 0.0 0.0 0.0 3.3991892151395744 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18140 0.0 30.72419 1 GGTATCA 12235 0.0 29.033989 1 GTACATG 10510 0.0 26.15262 1 ATCAACG 21825 0.0 25.414728 3 TCAACGC 22030 0.0 25.201557 4 CAACGCA 22105 0.0 25.093386 5 TACATGG 10630 0.0 25.084097 2 TATCAAC 22190 0.0 25.018105 2 AACGCAG 22390 0.0 24.783155 6 ACATGGG 10710 0.0 24.18666 3 AGTACTT 13060 0.0 23.512989 12-13 GAGTACT 12715 0.0 23.048956 12-13 ACGCAGA 24620 0.0 22.42156 7 CGCAGAG 24765 0.0 22.117636 8 AGAGTAC 19445 0.0 21.98503 10-11 GTACTTT 13525 0.0 21.580526 14-15 CATGGGG 3220 0.0 21.552418 4 GCAGAGT 25105 0.0 21.419725 9 CATGGGA 7435 0.0 19.946772 4 TACTTTT 15730 0.0 19.370703 14-15 >>END_MODULE