FastQCFastQC Report
Thu 26 May 2016
SRR937780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166296
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152790.7053052768412074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126340.5832074656464306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85220.39339037693833157No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72050.33259536092943903No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.13673108384080476No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26250.1211745763275194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.10303301118591364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA221600.025.3033981
GTACATG106300.024.9887851
TACATGG108050.024.3557322
GAGTACT124500.023.7893912-13
ACATGGG108850.023.085723
AGTACTT128850.022.98625612-13
GTACTTT132350.022.1789314-15
GGTATCA163800.021.6997281
TACGGTA1100.002877311521.5920312
TCAACGC259250.021.4379524
ATCAACG259700.021.4373873
CAACGCA260650.021.2863565
TATCAAC263050.021.236612
AACGCAG264700.020.994626
AGAGTAC198300.020.84072110-11
CATGGGG32650.020.8050634
TACTTTT149050.019.63020514-15
CGTCGAT857.962814E-519.55803544-45
CATGGGA73850.019.4898414
ACTTTTT151950.019.39622316-17
Produced by FastQC (version 0.11.5)