FastQCFastQC Report
Thu 26 May 2016
SRR937779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550381
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138290.5422327095441819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107190.420290144884235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70800.2776055812837376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32650.12802008797901177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.10469024039937562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83450.039.8868261
GTATCAA137050.038.963551
ATCAACG169550.031.400853
TATCAAC170550.031.2718662
TCAACGC170150.031.2640274
CAACGCA171750.030.889745
AACGCAG176450.030.0714196
ACGCAGA198200.026.46047
CGCAGAG200600.026.001748
GTACATG122700.024.9961411
TACATGG122500.024.524132
GCAGAGT208250.024.4758159
ACATGGG124550.023.1668053
GAGTACT111350.023.14206512-13
AGTACTT117700.021.59085812-13
GTACTTT121900.020.98395714-15
AGAGTAC184250.020.9465610-11
CCGTAAG2801.5570549E-920.3789141
CAGAGTA203300.020.07024210-11
CATGGGA77350.019.5440064
Produced by FastQC (version 0.11.5)