##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937779_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2550381 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.725613545583972 33.0 31.0 34.0 30.0 34.0 2 31.86198336640682 33.0 31.0 34.0 30.0 34.0 3 31.874883399774387 33.0 31.0 34.0 30.0 34.0 4 35.47439657055162 37.0 35.0 37.0 33.0 37.0 5 35.46845902631803 37.0 35.0 37.0 33.0 37.0 6 35.53590424332678 37.0 35.0 37.0 33.0 37.0 7 35.47562109347584 37.0 35.0 37.0 33.0 37.0 8 35.46613662821359 37.0 35.0 37.0 33.0 37.0 9 37.093192742574544 39.0 37.0 39.0 33.0 39.0 10-11 36.96947456085973 39.0 37.0 39.0 33.0 39.0 12-13 36.851124204579634 39.0 37.0 39.0 33.0 39.0 14-15 37.99188983920442 40.0 38.0 41.0 33.0 41.0 16-17 37.916521884377275 40.0 38.0 41.0 33.0 41.0 18-19 37.875911677510146 40.0 38.0 41.0 33.0 41.0 20-21 37.78889056184154 40.0 38.0 41.0 33.0 41.0 22-23 37.70324433878703 40.0 37.5 41.0 32.0 41.0 24-25 37.730409887777554 40.0 37.5 41.0 32.0 41.0 26-27 37.6449120739215 40.0 37.0 41.0 32.0 41.0 28-29 37.517347212044 40.0 37.0 41.0 32.0 41.0 30-31 37.26783331588496 39.5 37.0 41.0 31.5 41.0 32-33 37.11486068160012 39.0 36.0 41.0 31.0 41.0 34-35 37.1056595073442 40.0 36.0 41.0 31.0 41.0 36-37 36.97048715466434 40.0 36.0 41.0 31.0 41.0 38-39 36.8194777564607 39.5 36.0 41.0 30.5 41.0 40-41 36.583860411444405 39.0 35.5 41.0 30.0 41.0 42-43 36.302874550900434 39.0 35.0 41.0 30.0 41.0 44-45 36.03013608554957 39.0 35.0 41.0 29.5 41.0 46-47 35.63636668403662 39.0 35.0 40.5 27.5 41.0 48-49 35.28691615095941 39.0 35.0 40.0 26.0 41.0 50-51 34.38429728734648 38.0 34.0 39.5 24.5 40.5 52-53 34.46697395408765 38.0 34.0 39.5 24.0 40.5 54-55 34.75713001312353 38.0 34.0 40.0 24.0 41.0 56-57 34.53690056505283 38.0 34.0 40.0 23.5 41.0 58-59 34.29721912137833 38.0 34.0 40.0 23.0 41.0 60-61 33.981274758555685 38.0 34.0 40.0 22.0 41.0 62-63 33.620784698443096 37.5 33.0 40.0 21.5 41.0 64-65 33.234085024943326 37.0 33.0 40.0 19.5 41.0 66-67 32.79456481208102 36.0 32.0 39.0 18.0 41.0 68-69 32.28825359818788 36.0 31.5 39.0 14.5 40.0 70-71 31.83710277013513 35.0 31.0 38.5 10.5 40.0 72-73 31.381737669783455 35.0 31.0 38.0 7.5 40.0 74-75 30.849171751201094 35.0 30.0 37.0 6.5 39.0 76-77 30.36800168288581 34.5 30.0 36.5 2.0 39.0 78-79 29.814247557521796 34.0 29.0 36.0 2.0 38.5 80-81 29.25579354614075 34.0 29.0 35.5 2.0 37.5 82-83 28.76300462558339 34.0 28.5 35.0 2.0 37.0 84-85 28.253200404174905 34.0 27.5 35.0 2.0 36.5 86-87 27.728216882105066 33.0 26.5 35.0 2.0 36.0 88-89 27.280689042147035 33.0 26.0 35.0 2.0 36.0 90-91 26.73556049076589 33.0 25.0 35.0 2.0 35.0 92-93 26.118771077733093 32.0 24.5 35.0 2.0 35.0 94-95 25.390623597023346 32.0 21.5 34.0 2.0 35.0 96-97 24.579991969827255 31.5 14.5 34.0 2.0 35.0 98-99 23.563345437407193 31.0 2.0 34.0 2.0 35.0 100-101 20.79635826960756 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18545421726533462 1101 2 0.2717408860232169 1101 3 0.1837394946329134 1101 4 0.08815703459340796 1101 5 0.0833712585310451 1101 6 0.11603897492574333 1101 7 0.18310625822069682 1101 8 0.20992408948157504 1101 9 0.19304122522790124 1101 10-11 0.22876821219546883 1101 12-13 0.23406925647954324 1101 14-15 0.48484747249015214 1101 16-17 0.43271119188168683 1101 18-19 0.3889493165734734 1101 20-21 0.34195108416982833 1101 22-23 0.3769310206106695 1101 24-25 0.352287245681012 1101 26-27 0.3243630220336726 1101 28-29 0.36149316814077537 1101 30-31 0.46177356165568995 1101 32-33 0.3470078293792582 1101 34-35 0.41582349239929783 1101 36-37 0.4905293436943694 1101 38-39 0.45462789868236086 1101 40-41 0.513675404387925 1101 42-43 0.48740921502197665 1101 44-45 0.6855956667462948 1101 46-47 0.6874608462819225 1101 48-49 0.8685440075680049 1101 50-51 0.7868634378395072 1101 52-53 0.7526082050731802 1101 54-55 0.834969013719018 1101 56-57 0.7368841391156806 1101 58-59 0.6195382673622802 1101 60-61 0.7695809089279777 1101 62-63 0.854643731558518 1101 64-65 0.7624747738255664 1101 66-67 0.6953394563154305 1101 68-69 0.6866505462940964 1101 70-71 0.7264821790003282 1101 72-73 0.7337329012716687 1101 74-75 0.7848462346505833 1101 76-77 0.84692911202416 1101 78-79 0.8735630209404341 1101 80-81 0.9130666142907486 1101 82-83 0.7725140851072076 1101 84-85 0.8263549292238999 1101 86-87 0.8458505726287591 1101 88-89 0.911782299596009 1101 90-91 0.9653707283782573 1101 92-93 1.0403282090729071 1101 94-95 1.1199623676982497 1101 96-97 1.1997274469374908 1101 98-99 1.3849261401694406 1101 100-101 1.4505063699118672 1102 1 0.2115741824928037 1102 2 0.32315786660855395 1102 3 0.3041239242668361 1102 4 0.20730510761571708 1102 5 0.16325148648429888 1102 6 0.19399125928776328 1102 7 0.1836877321895969 1102 8 0.12269333511085989 1102 9 0.22107174778174965 1102 10-11 0.20030399892395678 1102 12-13 0.2704393907401439 1102 14-15 0.24543281007345996 1102 16-17 0.3556967999180003 1102 18-19 0.38246906930215374 1102 20-21 0.4049820896754781 1102 22-23 0.39808720722324153 1102 24-25 0.5116526470139533 1102 26-27 0.3153636981661947 1102 28-29 0.4976150651754452 1102 30-31 0.5195413584295707 1102 32-33 0.5788314553813834 1102 34-35 0.6054902556563277 1102 36-37 0.6938955177501995 1102 38-39 0.6620866063033404 1102 40-41 0.6617880287480133 1102 42-43 0.6688628999442159 1102 44-45 0.5576655981671408 1102 46-47 0.5483214663920251 1102 48-49 0.6017904095770774 1102 50-51 0.6394134233509519 1102 52-53 0.6763916494854243 1102 54-55 0.5765637205672718 1102 56-57 0.5759220991272755 1102 58-59 0.6327170561191693 1102 60-61 0.7561625760775996 1102 62-63 0.8050136726384025 1102 64-65 0.5643785698267223 1102 66-67 0.6073070019543749 1102 68-69 0.6506841597394768 1102 70-71 0.7202414758225046 1102 72-73 0.5385846350686307 1102 74-75 0.3463936122223039 1102 76-77 0.38388748157887775 1102 78-79 0.4672730566788701 1102 80-81 0.43382678042616973 1102 82-83 0.5347066862856131 1102 84-85 0.7083359975132844 1102 86-87 0.6989211801821256 1102 88-89 0.6831915529525254 1102 90-91 0.7341131218873862 1102 92-93 0.6915211000223884 1102 94-95 0.9533710415126038 1102 96-97 1.2176990493715643 1102 98-99 1.3145194947526555 1102 100-101 1.5021435765186482 1103 1 0.2826037462720059 1103 2 0.3029337364354987 1103 3 0.30666718759242784 1103 4 0.23063361702237728 1103 5 0.25695018159186134 1103 6 0.2417910622769739 1103 7 0.2347024593539757 1103 8 0.11779907639434839 1103 9 0.22401049202040468 1103 10-11 0.2583634426725112 1103 12-13 0.25661707744434636 1103 14-15 0.32259694924577076 1103 16-17 0.3269258067160976 1103 18-19 0.32123148888648956 1103 20-21 0.4118232892522684 1103 22-23 0.20963739024870165 1103 24-25 0.35765582107663363 1103 26-27 0.35754084977937595 1103 28-29 0.2867452990345498 1103 30-31 0.3149023624549159 1103 32-33 0.3338341308365145 1103 34-35 0.34293951321753013 1103 36-37 0.18199764716926126 1103 38-39 0.34409344087508487 1103 40-41 0.34412783587201545 1103 42-43 0.3642715149097455 1103 44-45 0.23953154570389046 1103 46-47 0.24269395055212328 1103 48-49 0.24975493547427163 1103 50-51 0.22028847959631292 1103 52-53 0.2946909721088318 1103 54-55 0.23877668679573105 1103 56-57 0.30709869132710566 1103 58-59 0.24230071881724058 1103 60-61 0.4050812665639256 1103 62-63 0.31704878190642916 1103 64-65 0.3133861583903652 1103 66-67 0.529965978714408 1103 68-69 0.5418901819457531 1103 70-71 0.36857849804094656 1103 72-73 0.33256285443162525 1103 74-75 0.3546498235346114 1103 76-77 0.4323440479970557 1103 78-79 0.4662729229061142 1103 80-81 0.4831966499369251 1103 82-83 0.6223808644952449 1103 84-85 0.644630370544693 1103 86-87 0.683517612097873 1103 88-89 0.7780917706372854 1103 90-91 0.8333971944165413 1103 92-93 0.6131238800634939 1103 94-95 0.8998568920033065 1103 96-97 1.2014021224966207 1103 98-99 1.359891991073905 1103 100-101 1.368902262626957 1104 1 0.13433373398781967 1104 2 0.18186194126167265 1104 3 0.1628007612807103 1104 4 0.11814813087153198 1104 5 0.11185112983000778 1104 6 0.09765539354611974 1104 7 0.07793448321558571 1104 8 0.17921632221267458 1104 9 0.21442661879992642 1104 10-11 0.13212818790187697 1104 12-13 0.29665610707783685 1104 14-15 0.24648127597144054 1104 16-17 0.12750578223186437 1104 18-19 0.23827642712900143 1104 20-21 0.30271647994321427 1104 22-23 0.23352581871435518 1104 24-25 0.22195238318749944 1104 26-27 0.2283202645136626 1104 28-29 0.35482839818306644 1104 30-31 0.34226701555288486 1104 32-33 0.3485551704488046 1104 34-35 0.3536382183297704 1104 36-37 0.29381765230169066 1104 38-39 0.3416818281129892 1104 40-41 0.39720776931865487 1104 42-43 0.2975816926938819 1104 44-45 0.391776038855042 1104 46-47 0.32728576432050716 1104 48-49 0.2996089556001351 1104 50-51 0.1599234878418656 1104 52-53 0.2032818561494878 1104 54-55 0.27597841667827794 1104 56-57 0.343279193127664 1104 58-59 0.36218274646507354 1104 60-61 0.3042599720127157 1104 62-63 0.42355151219283016 1104 64-65 0.3812602265591849 1104 66-67 0.41122928307613194 1104 68-69 0.14697886366756308 1104 70-71 0.29072668444083405 1104 72-73 0.3381727726492407 1104 74-75 0.2980813076493085 1104 76-77 0.35760719039263833 1104 78-79 0.4358130337161974 1104 80-81 0.5836940122025993 1104 82-83 0.45189730090354985 1104 84-85 0.37678674990726435 1104 86-87 0.5075122125252953 1104 88-89 0.6190612642091189 1104 90-91 0.7023368735973747 1104 92-93 0.6464528837824055 1104 94-95 0.8905462051827229 1104 96-97 0.8682488099734798 1104 98-99 0.8644535992083924 1104 100-101 0.7752784700109281 1105 1 0.09750373239495502 1105 2 0.18402889995339677 1105 3 0.11662485157840052 1105 4 0.11558516849851941 1105 5 0.09806787879464451 1105 6 0.11730016275782873 1105 7 0.12289804645228486 1105 8 0.09289913432552055 1105 9 0.13500188604709962 1105 10-11 0.13278247890041683 1105 12-13 0.15424162396219998 1105 14-15 0.12244584359736876 1105 16-17 0.220402106698252 1105 18-19 0.24837009637965224 1105 20-21 0.21865916781266748 1105 22-23 0.24758671478882377 1105 24-25 0.2639851873360186 1105 26-27 0.14966265062388828 1105 28-29 0.21126323677285797 1105 30-31 0.26815653052580757 1105 32-33 0.29418651978156873 1105 34-35 0.28453723517873186 1105 36-37 0.35396292370801774 1105 38-39 0.3981079280740616 1105 40-41 0.31934133317480473 1105 42-43 0.42235963197036597 1105 44-45 0.34857679369557815 1105 46-47 0.4588389062446865 1105 48-49 0.4613532301390961 1105 50-51 0.5230876236246687 1105 52-53 0.5282059185720556 1105 54-55 0.581228673306363 1105 56-57 0.5315833682011046 1105 58-59 0.4654896537426936 1105 60-61 0.5779444212357561 1105 62-63 0.7223313120229236 1105 64-65 0.622980375845934 1105 66-67 0.7990609638130977 1105 68-69 0.68584474452134 1105 70-71 0.5578303378513283 1105 72-73 0.5665695550640244 1105 74-75 0.4627798470815421 1105 76-77 0.35586278311502184 1105 78-79 0.423772807264033 1105 80-81 0.5687924379220455 1105 82-83 0.648407490808335 1105 84-85 0.6648495618431234 1105 86-87 0.797232321990446 1105 88-89 0.7513710165187746 1105 90-91 0.7631851068457536 1105 92-93 0.8014836238447032 1105 94-95 1.032634372415739 1105 96-97 1.2493487046674723 1105 98-99 1.229859082642605 1105 100-101 1.2471124316406055 1106 1 0.16818503855545686 1106 2 0.23902195137289795 1106 3 0.09603302817304993 1106 4 0.19082117566864554 1106 5 0.19462509452864651 1106 6 0.16442175284624483 1106 7 0.17705813138897497 1106 8 0.261983417873644 1106 9 0.29289507068369147 1106 10-11 0.16404929288275127 1106 12-13 0.1623921794170542 1106 14-15 0.21843911656581128 1106 16-17 0.12942533481022878 1106 18-19 0.35346881513621753 1106 20-21 0.226499698609409 1106 22-23 0.30156157617687995 1106 24-25 0.24677845915356045 1106 26-27 0.36618322446442875 1106 28-29 0.4056929352648879 1106 30-31 0.30266529609537685 1106 32-33 0.4889212077284455 1106 34-35 0.5631253210246285 1106 36-37 0.4458668923113649 1106 38-39 0.4225977181204641 1106 40-41 0.5317945417501875 1106 42-43 0.5532407493826739 1106 44-45 0.41663439929818225 1106 46-47 0.6595277220511946 1106 48-49 0.720079237395538 1106 50-51 0.7381425936580186 1106 52-53 0.6115029265104184 1106 54-55 0.6806361737451354 1106 56-57 0.5765750673088732 1106 58-59 0.5570116662127305 1106 60-61 0.47722165377295056 1106 62-63 0.644628055253456 1106 64-65 0.8517271425261228 1106 66-67 0.9254183107501106 1106 68-69 0.6730427402227122 1106 70-71 0.632566061611449 1106 72-73 0.7272597951137421 1106 74-75 0.8156450935331634 1106 76-77 0.7529986156355299 1106 78-79 0.7434075104603828 1106 80-81 0.6659527990755976 1106 82-83 0.7191571318702721 1106 84-85 0.7846564689910984 1106 86-87 0.9803935952351033 1106 88-89 0.9996392662533466 1106 90-91 0.973230768946209 1106 92-93 1.077186538220353 1106 94-95 1.2473793177909762 1106 96-97 1.47255171255404 1106 98-99 1.4252943831579223 1106 100-101 1.4864615381078217 1107 1 0.21550001000132468 1107 2 0.1743056237300067 1107 3 0.27853543411946546 1107 4 0.05379115694851322 1107 5 0.23336331609280592 1107 6 0.2229726278157642 1107 7 0.12832653716569098 1107 8 0.1656119825536635 1107 9 0.23084859827123694 1107 10-11 0.26910506726880357 1107 12-13 0.27048472063918894 1107 14-15 0.31697399266052884 1107 16-17 0.32263152690220664 1107 18-19 0.36350241430209707 1107 20-21 0.2902191541737409 1107 22-23 0.26785338785643376 1107 24-25 0.43643478146093884 1107 26-27 0.3384183353061374 1107 28-29 0.36058019785205175 1107 30-31 0.49083543530010587 1107 32-33 0.44262842908391775 1107 34-35 0.49068063103818815 1107 36-37 0.517337259995017 1107 38-39 0.5580273716106703 1107 40-41 0.5016156799864291 1107 42-43 0.49069206670999677 1107 44-45 0.545056614419984 1107 46-47 0.4655282747118008 1107 48-49 0.47829633746380296 1107 50-51 0.5066236636945618 1107 52-53 0.46623211134748743 1107 54-55 0.4397247844267014 1107 56-57 0.4945398430303314 1107 58-59 0.592461251411514 1107 60-61 0.6123053210850173 1107 62-63 0.7707249680744965 1107 64-65 0.648765783668992 1107 66-67 0.7901713486635416 1107 68-69 0.9542226782605141 1107 70-71 0.826516778503219 1107 72-73 0.8426754715649771 1107 74-75 0.9400596593123893 1107 76-77 0.8587435914308266 1107 78-79 0.8596630705089048 1107 80-81 1.0503891158334397 1107 82-83 1.0875482321235168 1107 84-85 1.1241848117660354 1107 86-87 1.0746331212464604 1107 88-89 1.1542806598447761 1107 90-91 1.4042356213521927 1107 92-93 1.475333500841998 1107 94-95 1.4108322273617908 1107 96-97 1.481802662511118 1107 98-99 1.7889957033517447 1107 100-101 1.7228944081897701 1108 1 0.02658408095375364 1108 2 0.016169139960094725 1108 3 -0.005210831539820049 1108 4 -0.0444427690047533 1108 5 -0.05797066352904068 1108 6 0.02637842246774369 1108 7 0.02485210674585403 1108 8 -0.1006602709717157 1108 9 0.009082401305562371 1108 10-11 -0.0403809670606492 1108 12-13 0.03316918717369788 1108 14-15 0.04723316544135514 1108 16-17 0.08012357196790987 1108 18-19 0.14508196247015093 1108 20-21 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-0.5257714935773024 2316 80-81 -0.5460849258444966 2316 82-83 -0.6731687222896632 2316 84-85 -0.7676067887533087 2316 86-87 -0.8522521612944374 2316 88-89 -0.9151673599262189 2316 90-91 -0.7226530354503815 2316 92-93 -0.8223868588542977 2316 94-95 -0.7493247447426796 2316 96-97 -0.9475916375626987 2316 98-99 -1.2188747620079212 2316 100-101 -1.1026164432362258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9469.0 3 1889.0 4 1844.0 5 1922.0 6 2268.0 7 2679.0 8 3405.0 9 4158.0 10 5498.0 11 7200.0 12 9850.0 13 12708.0 14 14567.0 15 18640.0 16 23720.0 17 31750.0 18 26757.0 19 17963.0 20 17831.0 21 18662.0 22 19886.0 23 22341.0 24 24719.0 25 28267.0 26 32477.0 27 38236.0 28 45416.0 29 54131.0 30 66380.0 31 81855.0 32 103536.0 33 133978.0 34 179810.0 35 250800.0 36 360754.0 37 483790.0 38 355575.0 39 35647.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72434865188488 19.151787557584658 15.07969933090706 22.044164459623396 2 14.845867571552851 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22.060849049099808 26.14435912609058 30.987878386746615 20.806913438062992 22-23 21.76595174705028 26.270330272908605 30.85466295131576 21.10905502872536 24-25 21.39079570980515 26.420141580593757 30.96369903115319 21.2253636784479 26-27 21.22486439287543 26.567742875654666 30.88405495105692 21.323337780412988 28-29 21.175097050040186 26.768938801709936 30.916340154792948 21.13962399345693 30-31 21.3077687329624 26.470620588000738 30.94360065889522 21.27801002014164 32-33 21.025662884684472 26.58671081871098 30.90328160501507 21.48434469158947 34-35 21.47596666069526 26.57539057712071 30.72860193662138 21.22004082556265 36-37 21.48306043381126 26.36055022953725 30.715159356353045 21.441229980298445 38-39 21.32322633083486 26.693322540591097 30.708375823864646 21.275075304709397 40-41 21.52944528901495 26.710706965412236 30.53136457303737 21.228483172535444 42-43 21.341350003545084 26.641280849846126 30.38638279017597 21.630986356432818 44-45 21.43708162079627 26.803727245336916 30.277033272354377 21.482157861512434 46-47 21.608605754414747 26.97995459304919 29.702014675649064 21.709424976887 48-49 21.62197304044326 27.221943971418078 29.40280830352776 21.753274684610897 50-51 21.773708366716036 27.420657843745953 29.207188312245673 21.59844547729233 52-53 21.770431088542182 27.58075150132097 29.00449178261532 21.64432562752152 54-55 21.665912177455862 27.40292375207321 29.068659172837602 21.862504897633325 56-57 21.761593929682345 27.797172997769188 28.84704810381123 21.594184968737235 58-59 21.946738920404112 27.239994156024714 29.089702664192927 21.723564259378243 60-61 21.681335212383825 27.27436421520221 29.315583283572007 21.728717288841963 62-63 21.613411970833194 27.310526416045477 29.44849080765645 21.627570805464877 64-65 21.59756829881066 27.242036272862986 29.43976143281697 21.720633995509388 66-67 21.572952513733366 27.37325786324073 29.147564322217367 21.906225300808536 68-69 21.617137151888592 27.424623648480928 29.221228865085326 21.737010334545158 70-71 21.60748532003329 27.40785426821677 29.14280227024492 21.84185814150502 72-73 21.610918016691212 27.44962199312715 28.979970544919 21.95948944526264 74-75 21.609333316314157 27.78377921938929 28.741697014803734 21.865190449492815 76-77 21.603849080688317 27.5146427081032 28.810781353560643 22.070726857647838 78-79 21.656124006040837 27.49853201388057 28.77720193008262 22.068142049995974 80-81 21.641742977300826 27.73677504897548 28.614681176291313 22.00680079743238 82-83 21.72756157238855 27.607739406721414 28.696424854390283 21.96827416649975 84-85 21.6941665568687 27.59778875081072 28.63148667883968 22.0765580134809 86-87 21.681758677963767 27.436842598212287 28.7734757075135 22.107923016310448 88-89 21.70224514244972 27.437503424059706 28.608880385984552 22.25137104750602 90-91 21.570530746004167 27.36761198860084 28.83217498405437 22.229682281340626 92-93 21.64262425903279 27.265492272419877 28.842622373969796 22.249261094577538 94-95 21.533554431583813 27.316401493424493 28.698445563703334 22.451598511288363 96-97 21.618500991747318 27.196478290640385 28.647290431646216 22.537730285966084 98-99 21.489069275736792 27.24579760108664 28.67174915349161 22.593383969684954 100-101 21.76786791674528 26.56700435647934 29.344085690057963 22.32104203671741 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3196.0 1 1866.5 2 572.0 3 598.0 4 531.5 5 548.5 6 894.0 7 1915.5 8 3281.0 9 3971.0 10 3817.5 11 3620.5 12 3793.0 13 4034.5 14 4264.5 15 4409.5 16 4596.0 17 4825.5 18 5102.5 19 5489.0 20 6035.0 21 6819.5 22 7964.0 23 9726.0 24 11785.5 25 14476.5 26 18150.5 27 22249.0 28 25773.0 29 29227.0 30 33317.5 31 37859.5 32 43027.0 33 48250.0 34 53592.0 35 58436.0 36 64517.5 37 70741.5 38 74549.5 39 76266.5 40 78798.0 41 84053.5 42 88538.5 43 91393.0 44 95898.5 45 100254.5 46 101786.0 47 103398.0 48 105480.5 49 102777.0 50 95818.5 51 88599.5 52 81584.0 53 77148.5 54 73936.5 55 68102.5 56 59991.5 57 52355.5 58 46305.0 59 40720.5 60 34034.5 61 27444.5 62 21590.5 63 16003.0 64 11562.0 65 8608.5 66 6243.0 67 4451.0 68 3286.5 69 2315.5 70 1597.0 71 1067.5 72 722.0 73 561.5 74 421.5 75 292.5 76 212.5 77 147.5 78 92.5 79 65.5 80 49.0 81 38.0 82 31.5 83 26.5 84 23.5 85 18.0 86 10.0 87 5.0 88 4.5 89 5.0 90 5.5 91 6.0 92 4.5 93 1.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2896037886104076 2 0.25729489044970144 3 0.25713805113824173 4 0.2588240737364339 5 0.26701892776020525 6 0.18550169562900604 7 0.18667799046495406 8 0.18906978996471507 9 0.18589379390765537 10-11 0.1940494381035618 12-13 0.18546248580114108 14-15 0.18548209071507354 16-17 0.19110870101369168 18-19 0.1852664366618164 20-21 0.17865958066657492 22-23 0.17828708730185805 24-25 0.1812474293056606 26-27 0.1770519777241126 28-29 0.18769744598944235 30-31 0.19244183516109947 32-33 0.19348089559952023 34-35 0.19279472361188385 36-37 0.17209193449919835 38-39 0.16568112764328152 40-41 0.17171944113448148 42-43 0.1804632327483619 44-45 0.18405093199800343 46-47 0.1856781398543982 48-49 0.184913548211032 50-51 0.18728574279686055 52-53 0.20738077957763956 54-55 0.17677750892905805 56-57 0.16550468341788935 58-59 0.16346577236891272 60-61 0.15917229621770237 62-63 0.1673279404136088 64-65 0.163897080475427 66-67 0.16148567606173353 68-69 0.17083722000752047 70-71 0.16562231290148413 72-73 0.1619757989100452 74-75 0.16599480626620103 76-77 0.16932764163472047 78-79 0.1715822067369542 80-81 0.1650341654835101 82-83 0.16432838858194126 84-85 0.1583096800046738 86-87 0.15470237584109983 88-89 0.15925071587343226 90-91 0.1658183620408088 92-93 0.1586821733693907 94-95 0.1628188102091413 96-97 0.16144646623386857 98-99 0.1641519443565491 100-101 0.1456449056042999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2550381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.691482632945615 #Duplication Level Percentage of deduplicated Percentage of total 1 70.53809748870886 34.346045488324926 2 14.746260137498885 14.360345387718503 3 5.921847491752773 8.65030602898898 4 2.9487931594084316 5.743244436379379 5 1.6092681388590182 3.9178825817503293 6 1.0448883873667352 3.052629886010036 7 0.6444390734351162 2.1965085766510266 8 0.456011989776899 1.776311990450948 9 0.3185084180561177 1.3957782395603793 >10 1.6075883025240865 13.84511356058174 >50 0.10782756944924217 3.611436793601576 >100 0.05365531851574393 4.7494044667559185 >500 0.0021712047285652562 0.7315767071473878 >1k 4.0207494973325985E-4 0.3850135817472755 >5k 8.041498994665198E-5 0.27721917611824576 >10k+ 1.6082997989330395E-4 0.9611830982133753 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13829 0.5422327095441819 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10719 0.420290144884235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7080 0.2776055812837376 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3265 0.12802008797901177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2670 0.10469024039937562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.920982786493469E-5 0.0 9 0.0 0.0 0.0 3.920982786493469E-5 0.0 10-11 0.0 0.0 0.0 3.920982786493469E-5 0.0 12-13 0.0 0.0 0.0 3.920982786493469E-5 0.0 14-15 0.0 0.0 0.0 3.920982786493469E-5 0.0 16-17 0.0 0.0 0.0 3.920982786493469E-5 0.0 18-19 0.0 0.0 0.0 5.881474179740204E-5 0.0 20-21 0.0 0.0 0.0 7.841965572986938E-5 0.0 22-23 0.0 0.0 0.0 2.548638811220755E-4 0.0 24-25 0.0 0.0 0.0 3.3328353685194487E-4 0.0 26-27 0.0 0.0 0.0 7.057769015688245E-4 0.0 28-29 0.0 0.0 0.0 0.001431158717070116 0.0 30-31 0.0 0.0 0.0 0.0030975764013298403 0.0 32-33 0.0 0.0 0.0 0.005430561159293454 0.0 34-35 0.0 0.0 0.0 0.008234063851636286 0.0 36-37 0.0 0.0 0.0 0.012135441724197286 0.0 38-39 0.0 0.0 0.0 0.017644422539220612 0.0 40-41 0.0 0.0 0.0 0.02768213847264389 0.0 42-43 0.0 0.0 0.0 0.03779827406179704 0.0 44-45 0.0 0.0 0.0 0.04930635854015537 0.0 46-47 0.0 0.0 0.0 0.06885245773082532 0.0 48-49 0.0 0.0 0.0 0.08537940017589529 0.0 50-51 0.0 0.0 0.0 0.10559206644026911 0.0 52-53 0.0 0.0 0.0 0.12849060591339098 0.0 54-55 0.0 0.0 0.0 0.16009372717252834 0.0 56-57 0.0 0.0 0.0 0.22204525519912516 0.0 58-59 0.0 0.0 0.0 0.2813893296727038 0.0 60-61 0.0 0.0 0.0 0.33189158796273965 0.0 62-63 0.0 0.0 0.0 0.38925556612913914 0.0 64-65 0.0 0.0 0.0 0.4489917388813671 0.0 66-67 0.0 0.0 0.0 0.5396448609050961 0.0 68-69 0.0 0.0 0.0 0.6470209745132198 0.0 70-71 0.0 0.0 0.0 0.7160890862973023 0.0 72-73 0.0 0.0 0.0 0.7954301729819976 0.0 74-75 0.0 0.0 0.0 0.9240776182068483 0.0 76-77 0.0 0.0 0.0 1.0772900205890807 0.0 78-79 0.0 0.0 0.0 1.2387364868229493 0.0 80-81 0.0 0.0 0.0 1.384008899062532 0.0 82-83 0.0 1.9604913932467345E-5 0.0 1.5848220324727953 0.0 84-85 0.0 3.920982786493469E-5 0.0 1.8628001071212497 0.0 86-87 0.0 3.920982786493469E-5 0.0 2.1082732344696735 0.0 88-89 0.0 3.920982786493469E-5 0.0 2.409169453505182 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8345 0.0 39.886826 1 GTATCAA 13705 0.0 38.96355 1 ATCAACG 16955 0.0 31.40085 3 TATCAAC 17055 0.0 31.271866 2 TCAACGC 17015 0.0 31.264027 4 CAACGCA 17175 0.0 30.88974 5 AACGCAG 17645 0.0 30.071419 6 ACGCAGA 19820 0.0 26.4604 7 CGCAGAG 20060 0.0 26.00174 8 GTACATG 12270 0.0 24.996141 1 TACATGG 12250 0.0 24.52413 2 GCAGAGT 20825 0.0 24.475815 9 ACATGGG 12455 0.0 23.166805 3 GAGTACT 11135 0.0 23.142065 12-13 AGTACTT 11770 0.0 21.590858 12-13 GTACTTT 12190 0.0 20.983957 14-15 AGAGTAC 18425 0.0 20.94656 10-11 CCGTAAG 280 1.5570549E-9 20.378914 1 CAGAGTA 20330 0.0 20.070242 10-11 CATGGGA 7735 0.0 19.544006 4 >>END_MODULE