FastQCFastQC Report
Thu 26 May 2016
SRR937779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550381
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148300.5814817472369814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125310.4913383529754966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82550.32367712902503587No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29750.1166492378981807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95550.042.1750641
GTATCAA150100.036.092311
ATCAACG190300.028.3131483
TATCAAC191850.028.1586972
TCAACGC192950.027.8996684
CAACGCA194850.027.6032335
AACGCAG199100.026.933446
GTACATG133100.023.8500331
ACGCAGA225950.023.6707617
CGCAGAG227450.023.3475888
TACATGG133250.023.177152
GAGTACT128200.023.10120412-13
ACATGGG133000.022.291893
TAGCGCG1503.0285993E-522.172814
GCAGAGT237950.021.9380579
AGTACTT134250.021.49404512-13
GTACTTT137200.021.3599514-15
AGAGTAC211150.020.5158710-11
CATGGGG51850.019.5184254
CAGAGTA233650.019.10945310-11
Produced by FastQC (version 0.11.5)