##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937779_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2550381 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16187267706276 33.0 31.0 34.0 31.0 34.0 2 32.44421755024053 34.0 31.0 34.0 31.0 34.0 3 32.529339341847354 34.0 31.0 34.0 31.0 34.0 4 36.15995217969394 37.0 35.0 37.0 35.0 37.0 5 36.078128718807115 37.0 35.0 37.0 35.0 37.0 6 36.11975975354271 37.0 35.0 37.0 35.0 37.0 7 36.0649871528999 37.0 35.0 37.0 35.0 37.0 8 36.0421984009448 37.0 35.0 37.0 35.0 37.0 9 37.737346694474276 39.0 37.0 39.0 35.0 39.0 10-11 37.659155632040864 39.0 37.0 39.0 35.0 39.0 12-13 37.557327513026486 39.0 37.0 39.0 35.0 39.0 14-15 38.778172751443805 40.0 38.0 41.0 35.0 41.0 16-17 38.74088106835802 40.0 38.0 41.0 35.0 41.0 18-19 38.736754822122656 40.0 38.0 41.0 35.0 41.0 20-21 38.65139424266413 40.0 38.0 41.0 34.0 41.0 22-23 38.59166689212318 40.0 38.0 41.0 34.0 41.0 24-25 38.61255828050789 40.0 38.0 41.0 34.0 41.0 26-27 38.48377732581916 40.0 38.0 41.0 34.0 41.0 28-29 38.3305443382773 40.0 38.0 41.0 34.0 41.0 30-31 38.36052554500681 40.0 38.0 41.0 34.0 41.0 32-33 38.275334744102935 40.0 38.0 41.0 34.0 41.0 34-35 38.13140134748494 40.0 38.0 41.0 34.0 41.0 36-37 37.98506928964731 40.0 37.0 41.0 33.0 41.0 38-39 37.85303411529493 40.0 37.0 41.0 33.0 41.0 40-41 37.663464400024935 40.0 37.0 41.0 33.0 41.0 42-43 37.43197486963712 40.0 37.0 41.0 33.0 41.0 44-45 37.113751435569824 40.0 36.5 41.0 31.5 41.0 46-47 36.71079889632176 40.0 36.0 41.0 31.0 41.0 48-49 36.352240312329805 39.5 36.0 41.0 30.0 41.0 50-51 36.08023977593936 39.0 36.0 41.0 29.5 41.0 52-53 35.84133782364282 39.0 35.0 40.5 29.0 41.0 54-55 35.61766928157009 39.0 35.0 40.0 28.0 41.0 56-57 35.353145275156926 38.5 35.0 40.0 28.0 41.0 58-59 35.096010556854054 38.0 34.5 40.0 27.0 41.0 60-61 34.773692832561096 38.0 34.0 40.0 26.0 41.0 62-63 34.41229839776881 37.5 34.0 40.0 26.0 41.0 64-65 34.00410762156713 37.0 33.5 40.0 25.5 41.0 66-67 33.57656307038046 36.5 33.0 39.0 24.5 40.5 68-69 33.130687532568665 36.0 32.5 39.0 23.0 40.0 70-71 32.73248742050697 35.5 32.0 38.5 22.5 40.0 72-73 32.244871060441554 35.0 31.5 38.0 21.5 40.0 74-75 31.769317211820507 35.0 31.0 37.0 20.0 39.0 76-77 30.530762266500574 34.0 29.5 36.0 19.5 38.5 78-79 31.12189570891565 34.0 31.0 36.0 20.0 38.5 80-81 30.93127693470113 34.0 31.0 36.0 18.5 37.5 82-83 30.562469881950975 34.0 31.0 35.0 17.5 37.0 84-85 30.1804001049255 34.0 30.0 35.0 12.5 36.5 86-87 29.81180929437602 34.0 30.0 35.0 7.5 36.0 88-89 29.386766918354553 34.0 29.5 35.0 4.5 36.0 90-91 28.8487400509963 34.0 29.0 35.0 2.0 35.0 92-93 28.404502503743558 34.0 29.0 35.0 2.0 35.0 94-95 27.94132033605959 33.0 28.0 35.0 2.0 35.0 96-97 27.380089876767432 33.0 27.0 35.0 2.0 35.0 98-99 26.491173671698462 33.0 25.5 34.5 2.0 35.0 100-101 23.72196820788737 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2958138259948626 1101 2 0.1876306004006807 1101 3 0.22367064527035296 1101 4 0.06384281876788123 1101 5 0.06650993691314255 1101 6 0.1148766358235136 1101 7 0.18698248611698887 1101 8 0.10120527243239508 1101 9 0.18994926075708918 1101 10-11 0.20909593539209936 1101 12-13 0.24044296790695086 1101 14-15 0.21721370941027374 1101 16-17 0.23519835278584367 1101 18-19 0.22446068335323588 1101 20-21 0.20970332053359186 1101 22-23 0.1300555946188453 1101 24-25 0.1897053716923125 1101 26-27 0.05261537331914923 1101 28-29 -0.01693009197455808 1101 30-31 0.10987458733682587 1101 32-33 0.17491581836917192 1101 34-35 0.13746645883827568 1101 36-37 0.007759739589580761 1101 38-39 0.04459793371709253 1101 40-41 0.07262781273244912 1101 42-43 0.13053420950257788 1101 44-45 0.1021000813673254 1101 46-47 0.0016137977859429498 1101 48-49 0.056866319023335166 1101 50-51 0.188873355764116 1101 52-53 0.13504240818183888 1101 54-55 -0.0018404391285287147 1101 56-57 0.00895138865504208 1101 58-59 -0.037227087642719425 1101 60-61 0.201694208927222 1101 62-63 -0.14703048325910828 1101 64-65 -0.11839570741284433 1101 66-67 -0.12941166385006397 1101 68-69 -0.06862606768193302 1101 70-71 -0.20934718650985218 1101 72-73 -0.28666779754234284 1101 74-75 -0.33452746510949893 1101 76-77 -0.362774718552334 1101 78-79 -0.38336292353320545 1101 80-81 -0.4461608593729345 1101 82-83 -0.4367352035361378 1101 84-85 -0.35521001198803503 1101 86-87 -0.4233636339556526 1101 88-89 -0.39742168369665976 1101 90-91 -0.31447475544775827 1101 92-93 -0.3017212227584096 1101 94-95 -0.4328032498296679 1101 96-97 -0.41750024652117546 1101 98-99 -0.40951202122759867 1101 100-101 -0.4947959377267921 1102 1 0.3117357809837529 1102 2 0.23088955915659426 1102 3 0.15896089930300406 1102 4 0.06698200547704403 1102 5 0.0791922512181813 1102 6 0.09743628037098517 1102 7 0.08139183606386524 1102 8 0.0937021332456851 1102 9 0.1292422193198206 1102 10-11 0.14504686748907858 1102 12-13 0.23388158473299114 1102 14-15 0.31281884801743587 1102 16-17 0.22244359571060102 1102 18-19 0.10475721575929953 1102 20-21 0.15208717062535726 1102 22-23 0.1644388651684494 1102 24-25 0.07877424061918958 1102 26-27 0.07626938664862593 1102 28-29 0.04194752803896762 1102 30-31 0.22323979548905726 1102 32-33 0.24252037743418242 1102 34-35 0.12813389822782995 1102 36-37 0.1719343749609763 1102 38-39 0.2428042507266568 1102 40-41 0.27473541439379545 1102 42-43 0.26046852805758647 1102 44-45 0.28911640513820913 1102 46-47 0.265874398577985 1102 48-49 0.18521752157332827 1102 50-51 0.1911248770622862 1102 52-53 0.2282067083846826 1102 54-55 0.19375310863540562 1102 56-57 0.3254383972515882 1102 58-59 0.189401908783438 1102 60-61 0.1654612329782239 1102 62-63 0.21306031167956263 1102 64-65 0.10181485957257053 1102 66-67 0.12704250402398287 1102 68-69 0.22848394873842182 1102 70-71 0.1123470084094933 1102 72-73 -0.017774723071177334 1102 74-75 0.0807454026582981 1102 76-77 0.08373933534508993 1102 78-79 0.24639463466249012 1102 80-81 0.2507038175323082 1102 82-83 0.2549870025876899 1102 84-85 0.288550250737746 1102 86-87 -0.009116555814046023 1102 88-89 0.024318874595625317 1102 90-91 0.19338673397559347 1102 92-93 0.1708345581746542 1102 94-95 -0.105569597748147 1102 96-97 -0.060559263231308336 1102 98-99 0.22256177380765507 1102 100-101 0.13947239029406688 1103 1 0.287889327785976 1103 2 0.20231715769239145 1103 3 0.1653414949565999 1103 4 0.10175076102557057 1103 5 0.07262644110252836 1103 6 0.12368103587087376 1103 7 0.07958578223857415 1103 8 0.13322274033077264 1103 9 0.17441484698159826 1103 10-11 0.13094661088860704 1103 12-13 0.154078413102539 1103 14-15 0.16896722632679229 1103 16-17 0.1757450465369601 1103 18-19 0.18861868798928327 1103 20-21 0.15522474982490309 1103 22-23 0.07337506772471869 1103 24-25 0.05757381254637295 1103 26-27 -0.06291228891596035 1103 28-29 -0.047346759864332455 1103 30-31 0.04775809195380276 1103 32-33 0.08109460307234428 1103 34-35 0.09935645961128614 1103 36-37 0.1578003711423719 1103 38-39 0.17965846274432096 1103 40-41 0.05365696275264753 1103 42-43 0.025101078617602468 1103 44-45 0.09149160123691047 1103 46-47 -0.05727370002470167 1103 48-49 -0.10820554084990874 1103 50-51 -0.15469235286876426 1103 52-53 -0.1280838338814334 1103 54-55 -0.2713202231575167 1103 56-57 -0.2923484541025658 1103 58-59 -0.3553599149040707 1103 60-61 -0.31378076193841054 1103 62-63 -0.3103573390276111 1103 64-65 -0.2931324218001805 1103 66-67 -0.2880803236888667 1103 68-69 -0.2836713127873196 1103 70-71 -0.3988062951695426 1103 72-73 -0.4086380437974384 1103 74-75 -0.3437901626532103 1103 76-77 -0.3554661760924809 1103 78-79 -0.2959485775862305 1103 80-81 -0.15823518209069576 1103 82-83 -0.22868147203805123 1103 84-85 -0.32748454234012314 1103 86-87 -0.42362329917709474 1103 88-89 -0.5252216159855614 1103 90-91 -0.5209585091867943 1103 92-93 -0.5288232267917223 1103 94-95 -0.5797748659625235 1103 96-97 -0.6865899823206796 1103 98-99 -0.5868528487707536 1103 100-101 -0.459584086131521 1104 1 0.2680100872178741 1104 2 0.18285860840420298 1104 3 0.17120330207806234 1104 4 0.08323298261208834 1104 5 0.0379882987069422 1104 6 0.05058802944270724 1104 7 0.07763377469978394 1104 8 0.05619627043544284 1104 9 0.08532610636301285 1104 10-11 0.10090662418244989 1104 12-13 0.15789644939977876 1104 14-15 0.08307363597913309 1104 16-17 0.05379558418040631 1104 18-19 0.09321431953103598 1104 20-21 0.10908254144810314 1104 22-23 0.085889055709778 1104 24-25 -0.05272486774507712 1104 26-27 0.042304966578264214 1104 28-29 -0.10007068331534441 1104 30-31 0.007866041336235696 1104 32-33 0.1258188189037952 1104 34-35 0.13135239783697727 1104 36-37 0.1323659022230359 1104 38-39 0.0583261019089889 1104 40-41 -0.09881207436914252 1104 42-43 -0.022482786189044646 1104 44-45 -0.07908267391201207 1104 46-47 -0.1073146648380785 1104 48-49 -0.06555875114506904 1104 50-51 -0.012228406111447043 1104 52-53 0.12355896556662316 1104 54-55 -0.07544478563858092 1104 56-57 -0.041537308728486266 1104 58-59 0.0851848382342908 1104 60-61 0.11989015861824726 1104 62-63 0.13919678080209508 1104 64-65 0.07334621339317238 1104 66-67 0.02608088100267736 1104 68-69 0.16963116634639164 1104 70-71 0.1618205769066563 1104 72-73 0.13751310426906826 1104 74-75 -0.04945087934287429 1104 76-77 -0.14831332001938335 1104 78-79 -0.037409596332235395 1104 80-81 -0.04886728125604023 1104 82-83 0.018645823674265927 1104 84-85 -0.01985982841019407 1104 86-87 0.013627178307508814 1104 88-89 -0.03140341141180514 1104 90-91 0.0804591152536851 1104 92-93 0.10305796788737354 1104 94-95 0.11361980766518442 1104 96-97 -0.0551815394673838 1104 98-99 0.08379925426495305 1104 100-101 0.056613842805305126 1105 1 0.23993641283466616 1105 2 0.12904248242635674 1105 3 0.08929856861730201 1105 4 0.04864681752058431 1105 5 0.037235559865841594 1105 6 0.035126187384022955 1105 7 0.07138620824196096 1105 8 0.03692482871656466 1105 9 0.061292665068897634 1105 10-11 0.07890684983818375 1105 12-13 0.15994421983268836 1105 14-15 0.10162939506730595 1105 16-17 0.14510485199393486 1105 18-19 0.14393376309055128 1105 20-21 0.013807429505156676 1105 22-23 0.10304386881374228 1105 24-25 0.024541479021401358 1105 26-27 0.08066561089174229 1105 28-29 0.04419661032027733 1105 30-31 0.010832750479274011 1105 32-33 0.02944004027649072 1105 34-35 0.11949800551457912 1105 36-37 0.0948434472656956 1105 38-39 0.12184182922838005 1105 40-41 0.2645622309651756 1105 42-43 0.1969900864526366 1105 44-45 0.059554288053938365 1105 46-47 0.005165037207866874 1105 48-49 -7.886434497592631E-4 1105 50-51 0.013758505856273473 1105 52-53 0.18238210457055004 1105 54-55 0.16540896732591648 1105 56-57 0.041035266136120185 1105 58-59 0.07708446125648294 1105 60-61 -0.02677915176015233 1105 62-63 -0.19406105741602175 1105 64-65 -0.03695878687231158 1105 66-67 -0.09599057222020946 1105 68-69 -0.06847517280974103 1105 70-71 -0.08358113453719085 1105 72-73 -0.0651232515236515 1105 74-75 -0.0018015704688529866 1105 76-77 -0.06115130584289474 1105 78-79 -0.02400852203398074 1105 80-81 -0.08430878111445494 1105 82-83 -0.15074889907891276 1105 84-85 -0.05283598412149715 1105 86-87 -0.09490023001432135 1105 88-89 0.07074258563254787 1105 90-91 0.19548546387851573 1105 92-93 0.3350605408051486 1105 94-95 0.209551975995506 1105 96-97 0.16166283108587365 1105 98-99 0.32754034971290125 1105 100-101 0.09699985790211585 1106 1 0.0926085232753735 1106 2 0.10841541259816267 1106 3 0.09450568810699167 1106 4 0.020044705037022936 1106 5 0.0567922893430719 1106 6 -0.029535173617034616 1106 7 0.07103949853149771 1106 8 -0.052859319312659636 1106 9 0.016900874464298 1106 10-11 -0.010916500845020494 1106 12-13 -0.19663484868348036 1106 14-15 0.024839308375490532 1106 16-17 0.11470265434351035 1106 18-19 0.12016292813876106 1106 20-21 0.2047988034826531 1106 22-23 0.16041605377545665 1106 24-25 0.03570884054612122 1106 26-27 0.031428875775084464 1106 28-29 0.14017837425269164 1106 30-31 0.2146872705093088 1106 32-33 0.11222705405567979 1106 34-35 -0.14338815861002985 1106 36-37 0.17421551425979231 1106 38-39 0.032229597424880296 1106 40-41 -0.19143692599793383 1106 42-43 -0.1692954153269497 1106 44-45 -0.03620304967585497 1106 46-47 0.061588446011704434 1106 48-49 0.07310548205051504 1106 50-51 0.3310270231759347 1106 52-53 0.3958448714003566 1106 54-55 0.32610747949528474 1106 56-57 0.3839477140217724 1106 58-59 0.35776611268806136 1106 60-61 0.4440019233564527 1106 62-63 0.42184428651713546 1106 64-65 0.13439925060686875 1106 66-67 -0.028690764708834138 1106 68-69 0.27770620096634957 1106 70-71 0.265956561871711 1106 72-73 0.33613029829464836 1106 74-75 0.2659320557694471 1106 76-77 0.3629041619562621 1106 78-79 0.2175017656056042 1106 80-81 0.02486737331379274 1106 82-83 0.0351723848691492 1106 84-85 -0.09710009635761452 1106 86-87 0.19188660537481184 1106 88-89 0.13301833925861573 1106 90-91 -0.18590636700770702 1106 92-93 -0.08099686626658098 1106 94-95 0.11162194805732284 1106 96-97 0.13280787946698425 1106 98-99 -0.08838038755594368 1106 100-101 -0.42690836616730365 1107 1 0.19583069513218732 1107 2 0.13303460803744827 1107 3 0.08818563569978721 1107 4 0.07494022313531445 1107 5 0.06884113139297199 1107 6 0.07922698128540162 1107 7 0.07614090754850622 1107 8 0.055609115205470516 1107 9 0.07274634964158366 1107 10-11 0.1427749356674255 1107 12-13 0.11659376705839719 1107 14-15 0.15711132440952724 1107 16-17 0.18330900940539863 1107 18-19 0.17404896157205485 1107 20-21 0.11550723335323454 1107 22-23 0.19671049116620054 1107 24-25 0.1618933726229912 1107 26-27 0.1530819539723538 1107 28-29 0.07640627069493888 1107 30-31 0.02607960832482803 1107 32-33 0.003239879145098712 1107 34-35 0.021275559259407828 1107 36-37 0.03830190053974292 1107 38-39 0.0071002610838917235 1107 40-41 -0.07823695611525494 1107 42-43 0.08249824734522093 1107 44-45 -0.03752975999990582 1107 46-47 -0.05779283782730005 1107 48-49 -0.18753180136652503 1107 50-51 -0.1022867924983828 1107 52-53 -0.007376650717155542 1107 54-55 -0.08427283202795621 1107 56-57 -0.04745051731141103 1107 58-59 -0.002268671296555169 1107 60-61 -0.0441335503455349 1107 62-63 -0.06639504992322287 1107 64-65 0.02841759771595065 1107 66-67 -0.1714255900474484 1107 68-69 -0.014122486476182416 1107 70-71 0.008844305265959918 1107 72-73 -0.01758749087947109 1107 74-75 -0.12658376920454373 1107 76-77 -0.11449245878784708 1107 78-79 -0.12415017363689174 1107 80-81 -0.03863298633602241 1107 82-83 -0.11611195863043733 1107 84-85 -0.07090633416067149 1107 86-87 -0.031145391610394313 1107 88-89 -0.06982262672791606 1107 90-91 -0.03012487204166092 1107 92-93 -0.10480446087817441 1107 94-95 0.05018345964140991 1107 96-97 0.10972980832129409 1107 98-99 -0.12288651306876375 1107 100-101 0.11501558452282978 1108 1 0.16872146484156048 1108 2 0.08259536262237077 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2316 86-87 -0.5493290338065933 2316 88-89 -0.5959775993102241 2316 90-91 -0.3078324131718233 2316 92-93 -0.011856704063756496 2316 94-95 -0.16894617314277127 2316 96-97 -0.05436407452325298 2316 98-99 -0.12021966716712029 2316 100-101 0.2384320172134764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 9.0 5 45.0 6 98.0 7 236.0 8 568.0 9 1137.0 10 2016.0 11 3401.0 12 5492.0 13 7583.0 14 9896.0 15 14436.0 16 20122.0 17 29272.0 18 26352.0 19 15052.0 20 12315.0 21 12155.0 22 12878.0 23 13679.0 24 15600.0 25 18726.0 26 22862.0 27 28264.0 28 35391.0 29 44328.0 30 55763.0 31 70702.0 32 90313.0 33 120403.0 34 167458.0 35 244454.0 36 380854.0 37 583770.0 38 449611.0 39 35131.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70162963381281 20.096450916860203 15.444657001966352 21.757262447360638 2 15.380564707782876 21.07387092359926 40.29648903438349 23.249075334234377 3 19.186152970869845 25.382050760258956 30.53504554809654 24.89675072077466 4 12.777110557206942 17.50918784291445 37.2031080846352 32.51059351524341 5 13.12739525778671 38.36428823852188 34.10243064612097 14.405885857570436 6 31.15832497183754 37.09069350814643 17.253618184890808 14.497363335125222 7 27.765341727373283 34.12960651761443 20.499290106066507 17.605761648945787 8 26.45220459217662 35.497088474231894 19.307507388111816 18.743199545479676 9 26.361473050497157 17.093798926513333 20.5839441244269 35.96078389856261 10-11 24.62243876503158 27.635753246279677 26.873886685950062 20.86792130273869 12-13 25.755270009567578 25.04189736028985 27.899505152375426 21.303327477767148 14-15 22.611601952806268 26.154954887132547 26.764118772842178 24.469324387219007 16-17 21.168856731602062 29.72475485035373 27.34132664884188 21.765061769202326 18-19 21.116197932779453 27.666532961153646 30.50146233053022 20.715806775536674 20-21 22.079583403420898 26.469731385232247 30.777362284301834 20.673322927045017 22-23 21.793745006837266 26.618608139039058 30.64370261090335 20.94394424322033 24-25 21.492084410215973 26.76302752709059 30.78882212973486 20.95606593295858 26-27 21.334626229293505 26.959449047521765 30.69156863752266 21.01435608566207 28-29 21.247431735127588 27.173046158189436 30.715604855785067 20.863917250897913 30-31 21.392627219227244 26.895354066706112 30.702373488510148 21.009645225556493 32-33 21.18473671188736 26.97051930672319 30.632403550685172 21.212340430704273 34-35 21.59455783273166 26.920389541797874 30.585724250611968 20.899328374858502 36-37 21.668429148429194 26.68881629842757 30.511323602238257 21.131430950904985 38-39 21.472262379620926 27.056859347681776 30.456919966075656 21.01395830662164 40-41 21.65698113251936 27.159993051980365 30.235746463156087 20.947279352344193 42-43 21.521203940877427 27.090650062821265 30.06181737756912 21.326328618732187 44-45 21.602811501497225 27.281159167983137 29.903963368610416 21.21206596190922 46-47 21.79219497008486 27.54204175768248 29.308091614547006 21.357671657685657 48-49 21.867713098552727 27.728955007114624 28.946420162320845 21.456911732011804 50-51 21.930017515030105 27.997130624796846 28.72498265945363 21.347869200719423 52-53 22.05756708507474 28.186965006404925 28.45057267914088 21.304895229379454 54-55 21.837610314551473 28.126992964766977 28.49768318611656 21.537713534564983 56-57 21.890690057681578 28.40012923559264 28.34956816256081 21.359612544164968 58-59 22.123635645027154 27.853936333434103 28.559909284142254 21.46251873739649 60-61 21.84969618264879 27.917848352853948 28.782327032706096 21.45012843179117 62-63 21.72479327598504 27.943432765535814 28.917875407635172 21.41389855084397 64-65 21.78623507624939 27.876678033595763 28.85319879657196 21.48388809358288 66-67 21.80076231747335 27.99679734125999 28.611195738989586 21.591244602277072 68-69 21.814544571967875 28.029968855633726 28.619057309476503 21.536429262921892 70-71 21.77682471756181 28.058690054544794 28.552714280729035 21.611770947164366 72-73 21.81260368548856 28.046221329283743 28.39309107149089 21.74808391373681 74-75 21.8080988307431 28.479083807298554 27.996724410337986 21.716092951620354 76-77 21.84810818462026 28.119661336874763 28.277422079289327 21.754808399215648 78-79 21.90890302272484 28.204668243685944 28.091606705037403 21.794822028551813 80-81 21.8872003830016 28.461806294824182 27.95427428294047 21.69671903923375 82-83 21.842098968889452 28.389535268983913 28.017430500906343 21.75093526122029 84-85 21.793482107270705 28.34366414880609 27.97971831250996 21.883135431413248 86-87 21.74808391373681 28.174653120455336 28.112681987514808 21.964580978293046 88-89 21.739281307381134 28.26956050880241 27.927886068787373 22.063272115029086 90-91 21.728883454598357 28.23531349433857 27.999319232238275 22.0364838188248 92-93 21.646951617355516 28.15650565258852 28.029306726643078 22.16723600341288 94-95 21.52241174946018 28.215176477553744 27.940982151294257 22.32142962169182 96-97 21.620553948606112 28.091783149262795 27.907810636920523 22.37985226521057 98-99 21.48319424768473 28.19731981569567 27.744597107766122 22.574888828853485 100-101 21.332712396994012 28.047472217183923 28.220615413273986 22.39919997254808 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 724.0 1 504.5 2 362.0 3 461.5 4 465.5 5 499.0 6 877.5 7 1870.5 8 3099.0 9 3928.0 10 3968.5 11 3854.5 12 4106.0 13 4513.0 14 4803.0 15 4933.5 16 5105.5 17 5337.5 18 5630.5 19 5940.0 20 6365.5 21 7076.5 22 8272.5 23 9896.0 24 12000.5 25 14698.0 26 18248.5 27 22230.0 28 25720.5 29 29322.0 30 33495.5 31 37871.5 32 42788.5 33 47765.0 34 53345.5 35 58135.5 36 64147.0 37 70950.5 38 74720.5 39 76453.0 40 78451.5 41 83862.0 42 88936.0 43 91571.0 44 96380.0 45 100044.0 46 99864.0 47 102294.0 48 106217.0 49 102925.0 50 95647.0 51 88879.0 52 81529.0 53 77403.5 54 74664.5 55 68503.5 56 60381.5 57 52612.5 58 46548.0 59 41305.0 60 34340.0 61 27241.0 62 21460.5 63 15862.5 64 11066.0 65 7999.0 66 5791.0 67 4337.0 68 3201.5 69 2215.5 70 1513.0 71 945.0 72 648.5 73 509.5 74 361.5 75 238.0 76 167.0 77 103.5 78 66.5 79 47.5 80 35.5 81 28.5 82 22.5 83 14.5 84 9.0 85 6.0 86 2.5 87 2.0 88 2.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018938346858763454 2 0.0 3 0.0 4 0.0 5 0.03183838022632697 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003960192614358404 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.253818155012918E-4 24-25 9.802456966233672E-5 26-27 0.0013331341474077795 28-29 8.234063851636285E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.489375901090857E-4 42-43 0.011331640252966126 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.3723439752727143E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.9604913932467345E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.410358687584325E-4 84-85 5.881474179740203E-5 86-87 0.0 88-89 0.0 90-91 0.012507935088914166 92-93 0.0017644422539220613 94-95 0.0 96-97 0.0 98-99 0.0013331341474077795 100-101 0.0183698043547219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2550381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.665041147414804 #Duplication Level Percentage of deduplicated Percentage of total 1 68.72535255552627 32.07071404874222 2 15.568759001913307 14.530335588769383 3 6.15156996287488 8.611897963162717 4 3.143620713795736 5.867887598445741 5 1.8203179808892962 4.247260673978903 6 1.0551062450545188 2.9541945804218064 7 0.7234157888275171 2.3630759286627936 8 0.5060358757775784 1.8891347972183024 9 0.3623779788018688 1.5219344962535593 >10 1.7667526316315698 14.56883124128509 >50 0.11422912471660154 3.682101073678168 >100 0.05868019124592037 4.965314299403123 >500 0.002941515846124369 0.8980723348941997 >1k 5.88303169223399E-4 0.4324269865288314 >5k 8.404330988905698E-5 0.32375156664207355 >10k+ 1.6808661977811397E-4 1.0730668219132373 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14830 0.5814817472369814 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12531 0.4913383529754966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8255 0.32367712902503587 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2975 0.1166492378981807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9604913932467345E-5 0.0 20-21 0.0 0.0 0.0 3.920982786493469E-5 0.0 22-23 0.0 0.0 0.0 2.156540532571408E-4 0.0 24-25 0.0 0.0 0.0 2.9407370898701016E-4 0.0 26-27 0.0 0.0 0.0 6.861719876363571E-4 0.0 28-29 0.0 0.0 0.0 0.0014507636310025834 0.0 30-31 0.0 0.0 0.0 0.0032152058849246445 0.0 32-33 0.0 0.0 0.0 0.005665820126483062 0.0 34-35 0.0 0.0 0.0 0.00856734738848823 0.0 36-37 0.0 0.0 0.0 0.012703984228238839 0.0 38-39 0.0 0.0 0.0 0.018428619096519307 0.0 40-41 0.0 0.0 0.0 0.02879961856679453 0.0 42-43 0.0 0.0 0.0 0.03926864260673209 0.0 44-45 0.0 0.0 0.0 0.05101198605228004 0.0 46-47 0.0 0.0 0.0 0.0706561098126123 0.0 48-49 0.0 0.0 0.0 0.08728107682734462 0.0 50-51 0.0 0.0 0.0 0.1078270266285704 0.0 52-53 0.0 0.0 0.0 0.13098042998281434 0.0 54-55 0.0 0.0 0.0 0.1629952544345335 0.0 56-57 0.0 0.0 0.0 0.225142831600455 0.0 58-59 0.0 0.0 0.0 0.28548675668458945 0.0 60-61 0.0 0.0 0.0 0.33667518696226173 0.0 62-63 0.0 0.0 0.0 0.39452928797697284 0.0 64-65 0.0 0.0 0.0 0.45453992952425537 0.0 66-67 0.0 0.0 0.0 0.5458792235356207 0.0 68-69 0.0 0.0 0.0 0.6536866452502587 0.0 70-71 0.0 0.0 0.0 0.723088040571193 0.0 72-73 0.0 0.0 0.0 0.8030760894156599 0.0 74-75 0.0 0.0 0.0 0.9328018049067963 0.0 76-77 0.0 0.0 0.0 1.0878570691986806 0.0 78-79 0.0 0.0 0.0 1.251558100534783 0.0 80-81 0.0 0.0 0.0 1.3985949550282881 0.0 82-83 0.0 0.0 0.0 1.6014077896596626 0.0 84-85 0.0 0.0 0.0 1.884032228910112 0.0 86-87 0.0 0.0 0.0 2.132583327745933 0.0 88-89 0.0 0.0 0.0 2.438478799834221 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9555 0.0 42.175064 1 GTATCAA 15010 0.0 36.09231 1 ATCAACG 19030 0.0 28.313148 3 TATCAAC 19185 0.0 28.158697 2 TCAACGC 19295 0.0 27.899668 4 CAACGCA 19485 0.0 27.603233 5 AACGCAG 19910 0.0 26.93344 6 GTACATG 13310 0.0 23.850033 1 ACGCAGA 22595 0.0 23.670761 7 CGCAGAG 22745 0.0 23.347588 8 TACATGG 13325 0.0 23.17715 2 GAGTACT 12820 0.0 23.101204 12-13 ACATGGG 13300 0.0 22.29189 3 TAGCGCG 150 3.0285993E-5 22.17281 4 GCAGAGT 23795 0.0 21.938057 9 AGTACTT 13425 0.0 21.494045 12-13 GTACTTT 13720 0.0 21.35995 14-15 AGAGTAC 21115 0.0 20.51587 10-11 CATGGGG 5185 0.0 19.518425 4 CAGAGTA 23365 0.0 19.109453 10-11 >>END_MODULE