FastQCFastQC Report
Thu 26 May 2016
SRR937778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468546
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138710.5619097233756227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106840.4328053842221291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68640.27805841981474116No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31050.1257825456766858No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.10573025578619966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79550.042.253581
GTATCAA135550.041.2118951
ATCAACG167200.032.9694673
TATCAAC169200.032.6954232
TCAACGC170250.032.353544
CAACGCA172100.031.9774935
AACGCAG176150.031.4434226
ACGCAGA199400.027.5871077
CGCAGAG200000.027.433098
GTACATG123900.025.7858961
GCAGAGT209900.025.5055249
TACATGG124350.024.7282162
GAGTACT113950.024.28212512-13
ACATGGG124000.023.914053
GTACTTT120100.022.62272514-15
AGTACTT117600.021.65023212-13
AGAGTAC187050.021.38255110-11
CAGAGTA205100.020.73982210-11
CATGGGG48550.020.2631344
CATGGGA78300.019.9691544
Produced by FastQC (version 0.11.5)