##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937778_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2468546 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.700712889287864 33.0 31.0 34.0 30.0 34.0 2 31.823126245166183 33.0 31.0 34.0 30.0 34.0 3 31.857638059003154 33.0 31.0 34.0 30.0 34.0 4 35.456066040495095 37.0 35.0 37.0 33.0 37.0 5 35.446553963345224 37.0 35.0 37.0 33.0 37.0 6 35.5260436710517 37.0 35.0 37.0 33.0 37.0 7 35.460832814134314 37.0 35.0 37.0 33.0 37.0 8 35.44793615350899 37.0 35.0 37.0 33.0 37.0 9 37.05564814267184 39.0 37.0 39.0 33.0 39.0 10-11 36.945868944714825 39.0 37.0 39.0 33.0 39.0 12-13 36.81919133773485 39.0 37.0 39.0 33.0 39.0 14-15 37.95124842721181 40.0 38.0 41.0 33.0 41.0 16-17 37.89979222586899 40.0 38.0 41.0 33.0 41.0 18-19 37.87573514935512 40.0 38.0 41.0 33.0 41.0 20-21 37.71053729604391 40.0 38.0 41.0 32.0 41.0 22-23 37.580857719483454 40.0 37.0 41.0 32.0 41.0 24-25 37.43556672632391 40.0 37.0 41.0 32.0 41.0 26-27 37.52644856526878 40.0 37.0 41.0 32.0 41.0 28-29 37.46407784177407 40.0 37.0 41.0 32.0 41.0 30-31 37.24569463157664 39.5 37.0 41.0 31.5 41.0 32-33 36.998403311098926 39.0 36.0 41.0 31.0 41.0 34-35 36.73691861524962 39.0 36.0 41.0 30.5 41.0 36-37 36.75700067975237 39.0 36.0 41.0 30.0 41.0 38-39 36.712798343640344 39.0 36.0 41.0 30.0 41.0 40-41 36.51732335552994 39.0 35.5 41.0 30.0 41.0 42-43 36.26237914950744 39.0 35.0 41.0 30.0 41.0 44-45 36.00082214388551 39.0 35.0 41.0 29.5 41.0 46-47 35.60390367447072 39.0 35.0 41.0 27.5 41.0 48-49 35.241406884862585 39.0 35.0 40.0 26.0 41.0 50-51 34.3353848783859 38.0 34.0 39.5 24.0 40.5 52-53 34.432991728734244 38.0 34.0 39.5 24.0 40.5 54-55 34.74608858818106 38.0 34.0 40.0 24.0 41.0 56-57 34.49883028308972 38.0 34.0 40.0 23.5 41.0 58-59 34.27545081193544 38.0 34.0 40.0 23.0 41.0 60-61 33.983815168929404 38.0 34.0 40.0 22.0 41.0 62-63 33.63040591506093 37.5 33.0 40.0 21.5 41.0 64-65 33.266624361061126 37.0 33.0 40.0 19.5 41.0 66-67 32.86293145843747 36.5 32.0 39.0 18.0 41.0 68-69 32.391898307748775 36.0 32.0 39.0 15.5 40.5 70-71 31.94903194025957 35.0 31.0 39.0 12.0 40.0 72-73 31.487390350433007 35.0 31.0 38.0 8.0 40.0 74-75 30.99950922526864 35.0 30.5 37.0 7.0 39.0 76-77 30.545195633380946 35.0 30.0 37.0 3.5 39.0 78-79 30.015241765800596 34.0 29.5 36.0 2.0 39.0 80-81 29.49059020978341 34.0 29.0 36.0 2.0 37.5 82-83 28.98389639083088 34.0 29.0 35.0 2.0 37.0 84-85 28.51800067732179 34.0 28.5 35.0 2.0 36.5 86-87 28.0014380935174 34.0 27.0 35.0 2.0 36.0 88-89 27.52766162753297 33.0 26.0 35.0 2.0 36.0 90-91 27.025376476678986 33.0 25.5 35.0 2.0 35.0 92-93 26.478254608178254 32.5 25.0 35.0 2.0 35.0 94-95 25.854115742627442 32.0 23.5 34.5 2.0 35.0 96-97 25.102166214443642 32.0 19.0 34.0 2.0 35.0 98-99 24.20057535893599 31.5 6.0 34.0 2.0 35.0 100-101 21.71365269271871 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1412913533593141 1101 2 0.21992998453623258 1101 3 0.21868383458546603 1101 4 0.16257072263356775 1101 5 0.12040926571298627 1101 6 0.18931204433877724 1101 7 0.19106394908835966 1101 8 0.3521123431091695 1101 9 0.33893220298326554 1101 10-11 0.23168653383135052 1101 12-13 0.3552520971297497 1101 14-15 0.5350813072304561 1101 16-17 0.5141260213983614 1101 18-19 0.4252216540862648 1101 20-21 0.4482781740514312 1101 22-23 0.427253074293759 1101 24-25 0.38740428432902974 1101 26-27 0.4597641887719419 1101 28-29 0.3683663100695469 1101 30-31 0.32613651899119134 1101 32-33 0.3180587227271232 1101 34-35 0.2808871876029855 1101 36-37 0.32960007074093767 1101 38-39 0.3454884811734402 1101 40-41 0.39308780353902506 1101 42-43 0.4188186279973962 1101 44-45 0.5115549423851178 1101 46-47 0.39187370587574577 1101 48-49 0.5497859958478628 1101 50-51 0.5427191680347434 1101 52-53 0.39483045831745045 1101 54-55 0.45086899398175007 1101 56-57 0.5954802299060518 1101 58-59 0.6247943038137649 1101 60-61 0.6723313025658015 1101 62-63 0.5500565161942248 1101 64-65 0.4622012180292785 1101 66-67 0.6186773610413638 1101 68-69 0.6740115773616608 1101 70-71 0.7321547975110541 1101 72-73 0.5289708896376091 1101 74-75 0.5636670359934364 1101 76-77 0.5810893023275057 1101 78-79 0.6095961415550626 1101 80-81 0.6265302320772612 1101 82-83 0.8456155931064764 1101 84-85 0.9642036372042213 1101 86-87 0.9512499830923709 1101 88-89 0.9735798710297416 1101 90-91 0.9999809684544303 1101 92-93 1.0738053342611629 1101 94-95 1.2776531278321848 1101 96-97 1.416431743530019 1101 98-99 1.7119765083956366 1101 100-101 2.008895296499208 1102 1 0.08916189182183842 1102 2 0.1482002696454856 1102 3 0.1818943803327251 1102 4 0.11115743473403938 1102 5 0.14370542568275368 1102 6 0.10458464096588926 1102 7 0.044732072839529735 1102 8 0.07666001366887798 1102 9 0.10061744048335441 1102 10-11 0.16042632662574619 1102 12-13 0.22637779309673078 1102 14-15 0.339371951143832 1102 16-17 0.39055929890411534 1102 18-19 0.31478264553766877 1102 20-21 0.2787249179781739 1102 22-23 0.37657058531284804 1102 24-25 0.2728071482793979 1102 26-27 0.38418363231257047 1102 28-29 0.3638496306346184 1102 30-31 0.370409982592534 1102 32-33 0.3131461788047645 1102 34-35 0.38042493571079916 1102 36-37 0.4395302435528379 1102 38-39 0.5007163523485971 1102 40-41 0.5247397739519286 1102 42-43 0.5262305135237142 1102 44-45 0.494245018320818 1102 46-47 0.4192240659987334 1102 48-49 0.6645092814032196 1102 50-51 0.49416943773938726 1102 52-53 0.4568428886690796 1102 54-55 0.5628004013120389 1102 56-57 0.5950311872779608 1102 58-59 0.581346109141883 1102 60-61 0.650780047106899 1102 62-63 0.6315155569329178 1102 64-65 0.597065427207788 1102 66-67 0.5947509186579438 1102 68-69 0.5795346414809543 1102 70-71 0.680530244073065 1102 72-73 0.6156371504484319 1102 74-75 0.5352785393399877 1102 76-77 0.653234416532861 1102 78-79 0.6667895160858599 1102 80-81 0.686283030725491 1102 82-83 0.6800433591036388 1102 84-85 0.8999733321343015 1102 86-87 0.9340984565024719 1102 88-89 0.8379557157301392 1102 90-91 1.0454960822091408 1102 92-93 1.317221270628476 1102 94-95 1.200559810892944 1102 96-97 1.4014854818757883 1102 98-99 1.5372696238264147 1102 100-101 1.8132069739434513 1103 1 0.2076098887234643 1103 2 0.27048705832223163 1103 3 0.1324998377960469 1103 4 0.1377460623243607 1103 5 0.12286019926545322 1103 6 0.22874197534953566 1103 7 0.1977048801564507 1103 8 0.17822983503494072 1103 9 0.21504778384974088 1103 10-11 0.26062713810830473 1103 12-13 0.2813944328264242 1103 14-15 0.4341630432367225 1103 16-17 0.371307782248401 1103 18-19 0.37092607054431426 1103 20-21 0.248271485343885 1103 22-23 0.26840582526362056 1103 24-25 0.2929310678251227 1103 26-27 -0.007926964934561909 1103 28-29 0.2522622911118404 1103 30-31 0.2441377420691424 1103 32-33 0.2775203773222614 1103 34-35 0.29037559702833704 1103 36-37 0.28379197931585765 1103 38-39 0.42225573326127375 1103 40-41 0.40755213361718035 1103 42-43 0.5009296796533249 1103 44-45 0.39223795497900227 1103 46-47 0.5676792173707739 1103 48-49 0.6083121869155761 1103 50-51 0.426732201459167 1103 52-53 0.3882062490182392 1103 54-55 0.5286514963227944 1103 56-57 0.6702671467940817 1103 58-59 0.6930716749605992 1103 60-61 0.8392933871492829 1103 62-63 0.5755357143137445 1103 64-65 0.6898848230515426 1103 66-67 0.7861840975253997 1103 68-69 0.8144051600245277 1103 70-71 0.9095870982353453 1103 72-73 0.8115711724743839 1103 74-75 0.7679640528298677 1103 76-77 0.7547950374164643 1103 78-79 0.7124971295727178 1103 80-81 0.8217043295795037 1103 82-83 0.6578826827542663 1103 84-85 0.8061160342458997 1103 86-87 0.9010990094076554 1103 88-89 0.928132492047574 1103 90-91 0.9086323339062972 1103 92-93 1.0677209992142451 1103 94-95 1.0902642081540073 1103 96-97 1.195455956651351 1103 98-99 1.4477128395439962 1103 100-101 1.6977461765420188 1104 1 0.17452715833932686 1104 2 0.2497975370491332 1104 3 0.23727696630522743 1104 4 0.22001703108114867 1104 5 0.2708456226275473 1104 6 0.25983914089884763 1104 7 0.31290511106369223 1104 8 0.36533246855477586 1104 9 0.3430346021527484 1104 10-11 0.36556292878010055 1104 12-13 0.31485786583537845 1104 14-15 0.41851354984265754 1104 16-17 0.38907479100536335 1104 18-19 0.42061354442008536 1104 20-21 0.543955367494128 1104 22-23 0.4744282724351834 1104 24-25 0.5400258684054648 1104 26-27 0.3782124049973348 1104 28-29 0.39809872637496113 1104 30-31 0.40843061832353555 1104 32-33 0.5141256055806025 1104 34-35 0.6473184084080827 1104 36-37 0.6248617728596599 1104 38-39 0.6702574309340505 1104 40-41 0.6758771805727761 1104 42-43 0.7071945101754267 1104 44-45 0.7856132717468327 1104 46-47 0.7602828399630752 1104 48-49 0.8674166496877191 1104 50-51 0.7435523446038488 1104 52-53 0.8676771830498993 1104 54-55 0.9116884212002816 1104 56-57 0.9305506314356364 1104 58-59 0.9994449101995784 1104 60-61 1.0842275132905215 1104 62-63 0.8745952792801504 1104 64-65 0.7722290014733844 1104 66-67 0.834814421364392 1104 68-69 1.007098872550948 1104 70-71 1.1772709422967758 1104 72-73 1.0523024826157545 1104 74-75 1.0660676875413415 1104 76-77 1.1238058808561107 1104 78-79 1.1088362629582527 1104 80-81 1.1822644651361038 1104 82-83 1.36869634387563 1104 84-85 1.4406082485464182 1104 86-87 1.4953208994932403 1104 88-89 1.4939323991504452 1104 90-91 1.407825543610933 1104 92-93 1.468228013244282 1104 94-95 1.4030322210705641 1104 96-97 1.4407097567523586 1104 98-99 1.6627183104824752 1104 100-101 1.64289556630483 1105 1 0.1879113756240045 1105 2 0.2798969484453977 1105 3 0.24088393565044797 1105 4 0.30649950981579366 1105 5 0.39425024794302743 1105 6 0.32871570395496263 1105 7 0.3125299992333126 1105 8 0.26853901210615305 1105 9 0.3988559483147114 1105 10-11 0.4015122727265492 1105 12-13 0.38917131923334836 1105 14-15 0.5167017759288086 1105 16-17 0.5271333885036071 1105 18-19 0.48809019932044606 1105 20-21 0.5121580814971836 1105 22-23 0.5881299554892152 1105 24-25 0.49695839208861514 1105 26-27 0.49836407782842684 1105 28-29 0.5072767554773634 1105 30-31 0.5259412280114333 1105 32-33 0.5409794342093974 1105 34-35 0.5850329415439504 1105 36-37 0.47859587996368447 1105 38-39 0.6742681120202931 1105 40-41 0.6854659936399727 1105 42-43 0.6684500827632149 1105 44-45 0.6987103810869328 1105 46-47 0.8299965758172689 1105 48-49 0.7919905042579174 1105 50-51 0.7259749612238764 1105 52-53 0.7543612945351938 1105 54-55 0.8100619179132522 1105 56-57 0.8854130083951688 1105 58-59 0.9512892866593177 1105 60-61 0.9647118673138735 1105 62-63 0.9925812695158882 1105 64-65 1.0841125145106218 1105 66-67 0.9549934094765646 1105 68-69 0.967777511243888 1105 70-71 1.1206978850082514 1105 72-73 1.0572434383368723 1105 74-75 1.1083092146656846 1105 76-77 1.038914382020664 1105 78-79 1.0654117948489805 1105 80-81 1.0466765483346627 1105 82-83 1.1199637999629353 1105 84-85 1.1402765977622806 1105 86-87 1.137717961450786 1105 88-89 0.9171727406293186 1105 90-91 0.9981883473379263 1105 92-93 1.0092339295719022 1105 94-95 1.216946936272251 1105 96-97 1.114083834929236 1105 98-99 1.1629495438025756 1105 100-101 1.3352910190948322 1106 1 0.039625196081132685 1106 2 0.15043120249933395 1106 3 0.043704573973855076 1106 4 -0.036604321866001044 1106 5 0.05675051267569131 1106 6 -0.031665758169445724 1106 7 -0.016785084410443574 1106 8 -0.014449619684825166 1106 9 0.10959315231417577 1106 10-11 -0.2725223943103501 1106 12-13 -0.3438253427818694 1106 14-15 -0.18753521157816522 1106 16-17 -0.10985782472502592 1106 18-19 -0.14935650116924393 1106 20-21 -0.03613415748228732 1106 22-23 -0.18812608335706926 1106 24-25 -0.16026089715711578 1106 26-27 -0.1348320455440586 1106 28-29 0.1556916245111779 1106 30-31 0.32290833291851584 1106 32-33 0.22941776444045558 1106 34-35 -0.03868457575779871 1106 36-37 -0.0335574084866721 1106 38-39 0.089032870572332 1106 40-41 -0.09116714218317412 1106 42-43 0.010744126631180961 1106 44-45 -0.03920604339837297 1106 46-47 0.2476076517614274 1106 48-49 0.4118502783056428 1106 50-51 0.3666783147396373 1106 52-53 0.3306926939473698 1106 54-55 0.2230404663310921 1106 56-57 0.24717946358945397 1106 58-59 0.21256189777056278 1106 60-61 0.05448639155985546 1106 62-63 0.2661364468632357 1106 64-65 0.32067659628830825 1106 66-67 0.5638294211200972 1106 68-69 0.5278499126868539 1106 70-71 0.2453937365885288 1106 72-73 0.34893383289443847 1106 74-75 0.48878756306328697 1106 76-77 0.5907291832829067 1106 78-79 0.5376879604374984 1106 80-81 0.4812128661268176 1106 82-83 0.4499951325448208 1106 84-85 0.5147059517910435 1106 86-87 0.387680457656284 1106 88-89 0.7704215693343421 1106 90-91 0.8505053170548642 1106 92-93 0.7667329235877638 1106 94-95 0.7330042961933714 1106 96-97 0.7543290538572691 1106 98-99 0.9721761243742577 1106 100-101 0.7135148746849254 1107 1 0.4563238595572372 1107 2 0.4346301103998442 1107 3 0.533586152689562 1107 4 0.4711565744991759 1107 5 0.4783099441426444 1107 6 0.4574662523627211 1107 7 0.43998101398040035 1107 8 0.38489116487819075 1107 9 0.5461568710414255 1107 10-11 0.5228968113421644 1107 12-13 0.631620024710692 1107 14-15 0.7506613745714219 1107 16-17 0.6180564165834923 1107 18-19 0.6731998072842416 1107 20-21 0.7374683436255864 1107 22-23 0.7846261204895129 1107 24-25 0.7698693089422903 1107 26-27 0.6670544800969012 1107 28-29 0.7494773633864895 1107 30-31 0.7945268195699455 1107 32-33 0.797149547142844 1107 34-35 0.8916883690637221 1107 36-37 0.9207304511683887 1107 38-39 1.0223093151393172 1107 40-41 1.0043779841814242 1107 42-43 0.9710884672415574 1107 44-45 0.9562163837865683 1107 46-47 1.0086531064601232 1107 48-49 1.0295197468314612 1107 50-51 1.030743375459906 1107 52-53 0.9267303214460654 1107 54-55 1.0709398398928798 1107 56-57 1.1874618983337584 1107 58-59 1.1488679875531673 1107 60-61 1.3351237267981375 1107 62-63 1.2605587942590617 1107 64-65 1.28751616434824 1107 66-67 1.544596404308571 1107 68-69 1.6666682733239213 1107 70-71 1.4566092020419 1107 72-73 1.4725361654337483 1107 74-75 1.4892061749798806 1107 76-77 1.46007515975748 1107 78-79 1.374152485933866 1107 80-81 1.4412802860576903 1107 82-83 1.6593081145338324 1107 84-85 1.7034799788976152 1107 86-87 1.7306092127175567 1107 88-89 1.7488347735716303 1107 90-91 1.7805322280884965 1107 92-93 1.8820012266680344 1107 94-95 1.953149400007252 1107 96-97 2.06515561291976 1107 98-99 2.1414593184262607 1107 100-101 2.152423662987914 1108 1 0.218865171418404 1108 2 0.24205978751092516 1108 3 0.2708816247418149 1108 4 0.3473183142634042 1108 5 0.21942255030636915 1108 6 0.1748204579511139 1108 7 0.23898247514335935 1108 8 0.22498346332249497 1108 9 0.20187987383742723 1108 10-11 0.31468654698061727 1108 12-13 0.398036177423549 1108 14-15 0.3649986394417155 1108 16-17 0.3250185579300293 1108 18-19 0.35533460901661584 1108 20-21 0.41126073155070486 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-0.5910572494809934 2316 94-95 -0.5777516878511584 2316 96-97 -0.2179490560786661 2316 98-99 -0.2962703852919901 2316 100-101 0.032432296504005365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9135.0 3 1853.0 4 1782.0 5 1974.0 6 2335.0 7 2704.0 8 3354.0 9 4194.0 10 5378.0 11 7131.0 12 9605.0 13 12298.0 14 14414.0 15 17776.0 16 22762.0 17 30194.0 18 25911.0 19 17293.0 20 16693.0 21 17604.0 22 19108.0 23 20866.0 24 23270.0 25 26705.0 26 30766.0 27 35974.0 28 42732.0 29 51129.0 30 62586.0 31 77631.0 32 98379.0 33 127957.0 34 172591.0 35 239766.0 36 344498.0 37 466567.0 38 360380.0 39 43244.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70311453347399 19.164225619760515 15.102710831505004 22.029949015260485 2 14.830295115624448 20.132354541571036 41.77354435992769 23.26380598287683 3 18.924906527028387 25.143945127873625 30.475396549539862 25.455751795558122 4 12.23082289517006 16.726643975183933 38.15811594096966 32.88441718867635 5 13.112637804420462 37.74760938670028 34.586249689346154 14.5535031195331 6 30.945311028709842 36.4841401009163 17.965650143841444 14.604898726532415 7 27.683256298880423 33.63541065944711 21.010174716493058 17.67115832517941 8 26.03001126183253 34.98732790195104 20.0088877164859 18.973773119730527 9 26.266187683986697 16.960970974899677 21.065267004703646 35.70757433640998 10-11 24.387376468697287 27.140681595682675 27.5308797815361 20.94106215408394 12-13 25.58179319850647 24.573483563067448 28.39292864168804 21.45179459673804 14-15 22.590591768812406 25.615187921097494 27.206692913385826 24.587527396704278 16-17 21.081696942642203 29.215638331870686 27.782297051199457 21.920367674287654 18-19 20.998392059811156 27.202415400511846 30.91989438405988 20.879298155617118 20-21 22.089841990486477 26.14623651026952 31.00182933609059 20.762092163153405 22-23 21.68761977115947 26.239897827743807 30.960920816501453 21.111561584595268 24-25 21.33817111014478 26.383341121907794 31.084305518551652 21.19418224939577 26-27 21.17891914859424 26.570311507629725 30.9680400208919 21.282729322884137 28-29 21.155226862161854 26.70568209628893 30.99912455756064 21.139966483988577 30-31 21.243635292198228 26.46778907928561 31.047821855552282 21.240753772963885 32-33 20.951518388611053 26.55266406965975 30.983634635381364 21.51218290634784 34-35 21.473294594997704 26.534745283775052 30.813048467562986 21.17891165366426 36-37 21.449464739349573 26.306478317682675 30.795241496294974 21.448815446672782 38-39 21.242532622730366 26.708498587477774 30.76806961247142 21.280899177320435 40-41 21.481076762360345 26.68452330110226 30.609121288436924 21.225278648100474 42-43 21.320892961821198 26.574726739893144 30.50369869264906 21.6006816056366 44-45 21.37811012000478 26.750334338598 30.378215022480987 21.49334051891623 46-47 21.5322610262973 27.000572399392336 29.81634724778563 21.650819326524736 48-49 21.621503152368113 27.18108806418541 29.487223716263326 21.710185067183147 50-51 21.724652238760537 27.443034121693604 29.262005499244108 21.570308140301748 52-53 21.72918392620919 27.558772420830707 29.070901152088418 21.641142500871688 54-55 21.57040826451211 27.41157332810643 29.17881830203178 21.839200105349683 56-57 21.72874632577903 27.740490036794956 28.95164392477403 21.57911971265198 58-59 21.852194732900735 27.219297588896985 29.21224803568722 21.71625964251506 60-61 21.63812330779721 27.256287295409052 29.384958206420237 21.7206311903735 62-63 21.52131917019102 27.27678301526586 29.56270574501556 21.639192069527564 64-65 21.603484038927228 27.260888230291304 29.487164921347002 21.648462809434466 66-67 21.52778144084436 27.373575850432193 29.197343440421562 21.901299268301884 68-69 21.575159522678685 27.42408432836139 29.33598272888418 21.664773420075743 70-71 21.535207538480112 27.368277963701438 29.242408718472586 21.85410577934587 72-73 21.55857319539439 27.410597482949694 29.08456659276307 21.946262728892844 74-75 21.564339781328847 27.76654921825621 28.84119710129342 21.82791389912152 76-77 21.630385207812253 27.48458470822533 28.871372598030543 22.013657485931873 78-79 21.636893083328022 27.484223849084593 28.828103157015665 22.050779910571716 80-81 21.601051759170684 27.713083182099 28.743417628936363 21.94244742979396 82-83 21.656003794248825 27.619327948951288 28.790364101018223 21.934304155781664 84-85 21.63797188566013 27.57794572523767 28.727677629593085 22.056404759509117 86-87 21.650096874909348 27.47032033127547 28.85748464414522 22.02209814966996 88-89 21.685137260017363 27.388952780346848 28.6743916612417 22.251518298394092 90-91 21.54803948459682 27.398931085355738 28.89055878624791 22.16247064379953 92-93 21.63899139615231 27.27511010936888 28.857479626470067 22.22841886800874 94-95 21.468526772717894 27.367493614143967 28.776895809567897 22.38708380357024 96-97 21.514363237318733 27.240080323925014 28.720875172092263 22.524681266663986 98-99 21.485531960997626 27.33925562102661 28.71565593218207 22.45955648579369 100-101 21.69137195767056 26.716770391222504 29.264769160201336 22.3270884909056 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3011.0 1 1786.0 2 589.0 3 615.5 4 511.5 5 499.0 6 891.0 7 1896.0 8 3357.0 9 4186.5 10 3928.5 11 3706.5 12 3936.0 13 4134.5 14 4269.5 15 4499.5 16 4649.0 17 4777.5 18 5041.0 19 5322.0 20 5829.0 21 6622.5 22 7915.5 23 9708.5 24 11512.5 25 14010.0 26 17609.5 27 21583.0 28 24988.5 29 28330.0 30 32286.0 31 36736.0 32 41658.5 33 46764.5 34 52188.0 35 56938.5 36 62681.0 37 68811.5 38 72494.5 39 74254.5 40 76455.0 41 81380.0 42 85767.5 43 88431.0 44 92847.0 45 96775.0 46 97955.5 47 99871.0 48 102286.5 49 99111.5 50 92417.5 51 85753.0 52 78643.5 53 74435.0 54 71103.0 55 64996.0 56 57658.5 57 50374.0 58 44444.0 59 39203.0 60 32798.5 61 26399.0 62 20654.5 63 15315.0 64 11182.5 65 8268.0 66 5897.5 67 4277.0 68 3132.0 69 2241.5 70 1555.5 71 1071.5 72 763.5 73 583.5 74 432.5 75 292.5 76 209.5 77 142.5 78 96.0 79 68.5 80 49.0 81 37.0 82 31.0 83 27.0 84 18.5 85 12.0 86 11.5 87 9.0 88 9.5 89 7.5 90 5.5 91 4.5 92 3.0 93 3.5 94 2.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27664058113561585 2 0.2397362658018121 3 0.23503714332242542 4 0.24095155609820518 5 0.24297703992552702 6 0.19128669265227385 7 0.18217201542932562 8 0.18111876383911824 9 0.19092210556335593 10-11 0.19436542806980303 12-13 0.19118541846090775 14-15 0.19225892488938834 16-17 0.17858690905496596 18-19 0.18326577669607938 20-21 0.17273326079400586 22-23 0.1811592735156647 24-25 0.18093647029465929 26-27 0.17957939613035365 28-29 0.18832948626438398 30-31 0.1850482024641226 32-33 0.18974732494350927 34-35 0.18547355406786017 36-37 0.17524486073988493 38-39 0.16852025443317645 40-41 0.16619094803175635 42-43 0.17680448328692275 44-45 0.17631836716836552 46-47 0.1765209155510977 48-49 0.18974732494350927 50-51 0.18516973149376192 52-53 0.19983423440357198 54-55 0.1770880510227478 56-57 0.16519846095636864 58-59 0.1674872576812423 60-61 0.1593245578571353 62-63 0.16313246745250037 64-65 0.1640641900130684 66-67 0.16224125456847877 68-69 0.16546177385392047 70-71 0.1670416512392315 72-73 0.16503642225018292 74-75 0.17595378007944756 76-77 0.16649477060585463 78-79 0.17115338340869482 80-81 0.16623145770830278 82-83 0.1524379128442411 84-85 0.15207332575532317 86-87 0.15270122574179293 88-89 0.15646862566061154 90-91 0.16163360942028224 92-93 0.1600537320349712 94-95 0.15877767722375843 96-97 0.1630514480994075 98-99 0.1694519769937445 100-101 0.15902073528303706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2468546.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.394112117135585 #Duplication Level Percentage of deduplicated Percentage of total 1 71.08054681773228 35.10960498862373 2 14.709668116511176 14.531419923056152 3 5.615155467227599 8.320668561101607 4 2.8346269930707493 5.600555340239819 5 1.622080387418024 4.0060610259566305 6 1.0111276802859575 2.9966252402870284 7 0.6303356623684447 2.1794409264919117 8 0.4444731446827453 1.7563485073212277 9 0.32467648627888396 1.4433396088551145 >10 1.570728697459718 13.62515319533697 >50 0.10265397180000159 3.480816139342771 >100 0.05113983075121522 4.573663023363885 >500 0.002131039845306676 0.7155306735198443 >1k 4.0981535486612315E-4 0.3887767940366686 >5k 8.196307097322464E-5 0.27788856755885594 >10k+ 1.6392614194644928E-4 0.9941074849078826 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13871 0.5619097233756227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10684 0.4328053842221291 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6864 0.27805841981474116 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3105 0.1257825456766858 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2610 0.10573025578619966 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.0509676546436645E-5 0.0 12-13 0.0 0.0 0.0 4.0509676546436645E-5 0.0 14-15 0.0 0.0 0.0 4.0509676546436645E-5 0.0 16-17 0.0 0.0 0.0 6.076451481965497E-5 0.0 18-19 0.0 0.0 0.0 8.101935309287329E-5 0.0 20-21 0.0 0.0 0.0 1.2152902963930994E-4 0.0 22-23 0.0 0.0 0.0 2.633128975518382E-4 0.0 24-25 0.0 0.0 0.0 4.6586128028402145E-4 0.0 26-27 0.0 0.0 0.0 8.507032074751696E-4 0.0 28-29 0.0 0.0 0.0 0.0020862483421414876 0.0 30-31 0.0 0.0 0.0 0.0039902031398240095 0.0 32-33 0.0 0.0 0.0 0.006967664365987104 0.0 34-35 0.0 0.0 0.0 0.01004639978351629 0.0 36-37 0.0 0.0 0.0 0.01415813195297961 0.0 38-39 0.0 0.0 0.0 0.02092324793623453 0.0 40-41 0.0 0.0 0.0 0.03315717025325839 0.0 42-43 0.0 0.0 0.0 0.04393274421461055 0.0 44-45 0.0 0.0 0.0 0.05507290526488062 0.0 46-47 0.0 0.0 0.0 0.07883183055936571 0.0 48-49 0.0 0.0 0.0 0.09566360116441014 0.0 50-51 0.0 0.0 0.0 0.11464238462641571 0.0 52-53 0.0 0.0 0.0 0.14066985180750124 0.0 54-55 0.0 0.0 0.0 0.1745359414003223 0.0 56-57 0.0 0.0 0.0 0.24303780444034667 0.0 58-59 0.0 0.0 0.0 0.31180298037792287 0.0 60-61 0.0 0.0 0.0 0.36551881147849785 0.0 62-63 0.0 0.0 0.0 0.42672893274016366 0.0 64-65 0.0 0.0 0.0 0.4915444152144623 0.0 66-67 0.0 0.0 0.0 0.5897803808395712 0.0 68-69 0.0 0.0 0.0 0.7069951299266856 0.0 70-71 0.0 0.0 0.0 0.780418918667102 0.0 72-73 0.0 0.0 0.0 0.8678388006543123 0.0 74-75 0.0 0.0 0.0 1.0103923524212228 0.0 76-77 0.0 0.0 0.0 1.1776365520431866 0.0 78-79 0.0 2.0254838273218323E-5 0.0 1.344394635546593 0.0 80-81 0.0 4.0509676546436645E-5 0.0 1.4943006936066818 0.0 82-83 0.0 4.0509676546436645E-5 0.0 1.700839279478689 0.0 84-85 0.0 4.0509676546436645E-5 0.0 1.9945141796020813 0.0 86-87 0.0 4.0509676546436645E-5 0.0 2.241704225888438 0.0 88-89 0.0 4.0509676546436645E-5 0.0 2.556484667492524 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7955 0.0 42.25358 1 GTATCAA 13555 0.0 41.211895 1 ATCAACG 16720 0.0 32.969467 3 TATCAAC 16920 0.0 32.695423 2 TCAACGC 17025 0.0 32.35354 4 CAACGCA 17210 0.0 31.977493 5 AACGCAG 17615 0.0 31.443422 6 ACGCAGA 19940 0.0 27.587107 7 CGCAGAG 20000 0.0 27.43309 8 GTACATG 12390 0.0 25.785896 1 GCAGAGT 20990 0.0 25.505524 9 TACATGG 12435 0.0 24.728216 2 GAGTACT 11395 0.0 24.282125 12-13 ACATGGG 12400 0.0 23.91405 3 GTACTTT 12010 0.0 22.622725 14-15 AGTACTT 11760 0.0 21.650232 12-13 AGAGTAC 18705 0.0 21.382551 10-11 CAGAGTA 20510 0.0 20.739822 10-11 CATGGGG 4855 0.0 20.263134 4 CATGGGA 7830 0.0 19.969154 4 >>END_MODULE