FastQCFastQC Report
Thu 26 May 2016
SRR937778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468546
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144210.5841900454761629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123190.499038705375553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80200.3248876059024219No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.11849080389832718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96450.038.6212351
GTATCAA149150.035.5829731
ATCAACG188700.027.9700263
TATCAAC190200.027.8743292
TCAACGC191250.027.6716164
CAACGCA194000.027.2793645
AACGCAG197950.026.7541356
ACGCAGA226700.023.2368897
CGCAGAG229600.022.7985748
GAGTACT126400.022.66043312-13
GTACATG129300.022.3785081
TACATGG131000.021.7585872
AGTACTT131450.021.46465112-13
GCAGAGT239300.021.3384939
GTACTTT135100.020.98894114-15
ACATGGG129300.020.905693
AGAGTAC208200.020.1112710-11
TACGACC1250.00602847819.00254
TACTTTT148900.018.852314-15
CATGGGG48900.018.458464
Produced by FastQC (version 0.11.5)