FastQCFastQC Report
Thu 26 May 2016
SRR937777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238621
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134800.6021564168298251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101800.45474423763558014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65530.2927248515939054No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29400.13133085055487284No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.11055913439568378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72600.044.9122161
GTATCAA120350.039.7309461
ATCAACG151250.031.447033
TCAACGC152600.031.201364
TATCAAC152750.031.14242
CAACGCA154700.030.7778135
AACGCAG159050.030.0447926
ACGCAGA179750.026.4262757
CGCAGAG180400.026.0677498
GTACATG105450.025.0163211
CTATCGG1151.2766142E-424.7987861
TACATGG103650.024.437522
GCAGAGT190050.023.9948449
GAGTACT107550.023.75979412-13
ACATGGG107600.022.2566173
GTACTTT115550.021.92836214-15
AGTACTT111750.021.67671612-13
AGAGTAC166850.021.3084110-11
CAGAGTA184900.020.29437310-11
CATGGGG43700.020.1164174
Produced by FastQC (version 0.11.5)