##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937777_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2238621 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.805646869211 33.0 31.0 34.0 30.0 34.0 2 31.935921265814983 33.0 31.0 34.0 30.0 34.0 3 31.944705244880666 34.0 31.0 34.0 30.0 34.0 4 35.54331885567052 37.0 35.0 37.0 33.0 37.0 5 35.54182641903208 37.0 35.0 37.0 33.0 37.0 6 35.59382896881607 37.0 35.0 37.0 33.0 37.0 7 35.53917523332444 37.0 35.0 37.0 33.0 37.0 8 35.52417403392535 37.0 35.0 37.0 33.0 37.0 9 37.151590644419045 39.0 37.0 39.0 34.0 39.0 10-11 37.04515547741221 39.0 37.0 39.0 33.0 39.0 12-13 36.92540474694019 39.0 37.0 39.0 33.0 39.0 14-15 38.08356126383162 40.0 38.0 41.0 33.0 41.0 16-17 38.02681338198828 40.0 38.0 41.0 33.0 41.0 18-19 37.99607459234949 40.0 38.0 41.0 33.0 41.0 20-21 37.92705844356861 40.0 38.0 41.0 33.0 41.0 22-23 37.73860693703847 40.0 37.5 41.0 32.5 41.0 24-25 37.57273607278766 40.0 37.0 41.0 32.0 41.0 26-27 37.51898847549451 40.0 37.0 41.0 32.0 41.0 28-29 37.555514979980984 40.0 37.0 41.0 32.0 41.0 30-31 37.358460856035926 40.0 37.0 41.0 32.0 41.0 32-33 37.10867516207522 39.0 36.0 41.0 31.0 41.0 34-35 37.13296377546713 39.5 36.0 41.0 31.0 41.0 36-37 37.047254984206795 40.0 36.0 41.0 31.0 41.0 38-39 36.91929026842864 40.0 36.0 41.0 31.0 41.0 40-41 36.67996592545143 39.5 36.0 41.0 30.5 41.0 42-43 36.41813553969162 39.0 35.5 41.0 30.0 41.0 44-45 36.15165519308539 39.0 35.0 41.0 29.5 41.0 46-47 35.76136693080249 39.0 35.0 41.0 27.5 41.0 48-49 35.37404433354284 39.0 35.0 40.5 26.5 41.0 50-51 34.42860627145015 38.0 34.0 39.5 25.0 40.5 52-53 34.51732852501607 38.0 34.5 39.5 24.0 40.5 54-55 34.881503389810064 39.0 35.0 40.0 25.0 41.0 56-57 34.69649306425697 38.5 34.0 40.0 24.0 41.0 58-59 34.46833832077873 38.0 34.0 40.0 23.0 41.0 60-61 34.16077442318284 38.0 34.0 40.0 22.5 41.0 62-63 33.82507199744843 38.0 34.0 40.0 22.0 41.0 64-65 33.462075760032626 37.0 33.0 40.0 20.5 41.0 66-67 33.06259165798945 36.5 32.5 39.5 19.5 41.0 68-69 32.5857195121461 36.0 32.0 39.0 17.5 40.5 70-71 32.150411570337276 35.5 31.5 39.0 13.5 40.0 72-73 31.704917893649707 35.0 31.0 38.0 9.5 40.0 74-75 31.254688489029633 35.0 31.0 37.0 7.5 39.5 76-77 30.768287932615657 35.0 30.5 37.0 6.0 39.0 78-79 30.25133106497259 34.5 30.0 36.0 2.0 39.0 80-81 29.725197342471102 34.0 29.5 36.0 2.0 37.5 82-83 29.204299879256027 34.0 29.0 35.0 2.0 37.0 84-85 28.752505225315048 34.0 29.0 35.0 2.0 36.5 86-87 28.29379783357701 34.0 27.5 35.0 2.0 36.0 88-89 27.8273709573885 34.0 27.0 35.0 2.0 36.0 90-91 27.347352901630067 33.0 26.0 35.0 2.0 35.0 92-93 26.804345845053717 33.0 25.0 35.0 2.0 35.0 94-95 26.19416841886143 32.5 24.5 35.0 2.0 35.0 96-97 25.49979876004022 32.0 21.5 34.0 2.0 35.0 98-99 24.59490373761347 32.0 10.0 34.0 2.0 35.0 100-101 22.269635637296354 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16849704121911913 1101 2 0.33902662958965735 1101 3 0.24479544511507711 1101 4 0.16594452356630285 1101 5 0.17547921624308316 1101 6 0.2501022625930318 1101 7 0.19351190743284974 1101 8 0.1245843750371094 1101 9 0.3105307324918627 1101 10-11 0.24744703578909366 1101 12-13 0.3867787283356847 1101 14-15 0.5442991614112813 1101 16-17 0.6087395662910282 1101 18-19 0.5401129678054062 1101 20-21 0.5644251177362989 1101 22-23 0.5523406132325448 1101 24-25 0.47362865009370836 1101 26-27 0.4793813133725706 1101 28-29 0.29886241137939606 1101 30-31 0.4478815765474238 1101 32-33 0.5149228131121717 1101 34-35 0.645852299691704 1101 36-37 0.6452604208508745 1101 38-39 0.6018519702731879 1101 40-41 0.6595005122718263 1101 42-43 0.6323298986472068 1101 44-45 0.7714488189320363 1101 46-47 0.7789167417004847 1101 48-49 0.9337483906923936 1101 50-51 0.7695239136287597 1101 52-53 0.8264870228552894 1101 54-55 0.8790382895956412 1101 56-57 0.889322005362196 1101 58-59 0.9913464470720612 1101 60-61 0.9598907027300001 1101 62-63 1.0781501985367896 1101 64-65 0.9777171740516337 1101 66-67 1.0261770528164718 1101 68-69 0.8421576652431071 1101 70-71 0.9320559975970824 1101 72-73 1.062642961633756 1101 74-75 1.1206264029898279 1101 76-77 1.141076223472428 1101 78-79 1.2518439050233745 1101 80-81 1.0734560876547974 1101 82-83 1.2844738348741842 1101 84-85 1.4197441657265202 1101 86-87 1.5350885762029591 1101 88-89 1.406426854483886 1101 90-91 1.3464177635618917 1101 92-93 1.4043184493782555 1101 94-95 1.490550152277958 1101 96-97 1.6236494958660792 1101 98-99 1.699557375898216 1101 100-101 2.2021675778650476 1102 1 0.1512827097220395 1102 2 0.012516389377516646 1102 3 0.16135424042088786 1102 4 0.19779909156857656 1102 5 0.1682513601406299 1102 6 0.04827003887510983 1102 7 0.05431282901584211 1102 8 0.14474868905060845 1102 9 0.18687222225519662 1102 10-11 0.09633157562893047 1102 12-13 0.14659578205378665 1102 14-15 0.3360688077084646 1102 16-17 0.2321110950146945 1102 18-19 0.3479908762952846 1102 20-21 0.31519421172705364 1102 22-23 0.36703135645767304 1102 24-25 0.25629638515227526 1102 26-27 0.2852518077089954 1102 28-29 0.16460648187401716 1102 30-31 0.4046351975993545 1102 32-33 0.3172709449904332 1102 34-35 0.4902853332648647 1102 36-37 0.44969987034789227 1102 38-39 0.4249247013430164 1102 40-41 0.5140874482299935 1102 42-43 0.49344002824608424 1102 44-45 0.663631536236629 1102 46-47 0.6109808214048513 1102 48-49 0.6726144641710619 1102 50-51 0.653327872580526 1102 52-53 0.5946376853633879 1102 54-55 0.5687937334741022 1102 56-57 0.5483019747871367 1102 58-59 0.5638613309135749 1102 60-61 0.7403643463402503 1102 62-63 0.7627260440399226 1102 64-65 0.6916221882367068 1102 66-67 0.6854669268143638 1102 68-69 0.7906710161524728 1102 70-71 0.8143610290567622 1102 72-73 0.8168312643128814 1102 74-75 0.8782201098626601 1102 76-77 0.8175100767032966 1102 78-79 0.8931795139453307 1102 80-81 1.0224978720871043 1102 82-83 0.9409742510986945 1102 84-85 1.0144495214525193 1102 86-87 1.055294556993939 1102 88-89 1.0910924292023587 1102 90-91 1.1480360302771366 1102 92-93 1.162212245701678 1102 94-95 1.401755273264225 1102 96-97 1.585602345538046 1102 98-99 1.5817465255907344 1102 100-101 2.097639564302696 1103 1 0.1934432399805388 1103 2 0.17638631131799443 1103 3 0.21885381141667537 1103 4 0.19405278654571845 1103 5 0.17343524402163268 1103 6 0.1557078239737919 1103 7 0.09663155665238321 1103 8 0.2380745248191687 1103 9 0.21665380768224907 1103 10-11 0.22057557597345578 1103 12-13 0.31049098637723915 1103 14-15 0.3429357285438144 1103 16-17 0.36518116862292516 1103 18-19 0.18813348751553605 1103 20-21 0.21199139167298853 1103 22-23 0.33425662886041607 1103 24-25 0.3706892144579683 1103 26-27 0.21861474554047788 1103 28-29 0.19429151646841092 1103 30-31 0.24739058715973528 1103 32-33 0.25759184677919933 1103 34-35 0.14017456192126332 1103 36-37 0.22578130271366348 1103 38-39 0.41084951432305417 1103 40-41 0.44711687095156805 1103 42-43 0.49136236050012627 1103 44-45 0.3061230467309386 1103 46-47 0.3606472067580029 1103 48-49 0.39866325505602873 1103 50-51 0.463458702608456 1103 52-53 0.36971246746706754 1103 54-55 0.43096957649558476 1103 56-57 0.34131888770598806 1103 58-59 0.44127574872000963 1103 60-61 0.4942382909002987 1103 62-63 0.6415053787376692 1103 64-65 0.6056308978517393 1103 66-67 0.48031983178670856 1103 68-69 0.5841033299045861 1103 70-71 0.7079016257289652 1103 72-73 0.6506191054375847 1103 74-75 0.6849877857321545 1103 76-77 0.7292599613399808 1103 78-79 0.7674731832233626 1103 80-81 0.6883330513883017 1103 82-83 0.8911852760491534 1103 84-85 0.957098791362327 1103 86-87 0.9956219618628346 1103 88-89 1.0557661716626008 1103 90-91 1.156418500574322 1103 92-93 0.9646405090965935 1103 94-95 1.0003824465040232 1103 96-97 1.3372461981166701 1103 98-99 1.5437948816983393 1103 100-101 1.758135913884928 1104 1 0.22415063563655835 1104 2 0.31849741220441885 1104 3 0.3096670984111647 1104 4 0.23242449536960663 1104 5 0.2211737463963459 1104 6 0.17984508801288257 1104 7 0.24656356248521405 1104 8 0.2188039315646364 1104 9 0.4090567813573216 1104 10-11 0.401281437926464 1104 12-13 0.3894666772544255 1104 14-15 0.4861188108050669 1104 16-17 0.5854610505151854 1104 18-19 0.6146976860128461 1104 20-21 0.5953765277516609 1104 22-23 0.45375261342982753 1104 24-25 0.5710997286294557 1104 26-27 0.5662016424222642 1104 28-29 0.4764077829226281 1104 30-31 0.5954643472098624 1104 32-33 0.5521565293383901 1104 34-35 0.5794246553790998 1104 36-37 0.6345338867231263 1104 38-39 0.7598815936475773 1104 40-41 0.6739801905600089 1104 42-43 0.7804924819652044 1104 44-45 0.7796660393679105 1104 46-47 0.7487863319702157 1104 48-49 0.835340229047361 1104 50-51 0.8940591312329289 1104 52-53 0.9103036336458175 1104 54-55 0.9199896635230758 1104 56-57 0.992716315258825 1104 58-59 0.8841953842225294 1104 60-61 1.0926112631033007 1104 62-63 1.0686656904813674 1104 64-65 0.9534053407008614 1104 66-67 0.9433462438334672 1104 68-69 0.9312425115626723 1104 70-71 0.9623115121326933 1104 72-73 0.9901296902994758 1104 74-75 1.1737051239856626 1104 76-77 1.1898857429857728 1104 78-79 1.2155520836297278 1104 80-81 1.196724017639415 1104 82-83 1.3552753356506777 1104 84-85 1.3481589555203755 1104 86-87 1.457135050232285 1104 88-89 1.541351184120611 1104 90-91 1.4249300508987552 1104 92-93 1.624477645243065 1104 94-95 1.5500982352272565 1104 96-97 1.5907397144747009 1104 98-99 1.9001388726569175 1104 100-101 2.3854322839950584 1105 1 0.19222771621756962 1105 2 0.03589529058535845 1105 3 0.12477872144838287 1105 4 0.10137579870110613 1105 5 0.041248965042804286 1105 6 0.045569623054937836 1105 7 0.050510688263678105 1105 8 0.11535688679575884 1105 9 0.13658032821860644 1105 10-11 0.2544817098040042 1105 12-13 0.20228227236323448 1105 14-15 0.22036819536671004 1105 16-17 0.2594374138701738 1105 18-19 0.18170236913829996 1105 20-21 0.19478217004268572 1105 22-23 0.25444914457071377 1105 24-25 0.20165034116326552 1105 26-27 0.23908471677454912 1105 28-29 0.2092202495941038 1105 30-31 0.23820542711677462 1105 32-33 0.3213733867746882 1105 34-35 0.27728152453038746 1105 36-37 0.21972790656692354 1105 38-39 0.34865296947471336 1105 40-41 0.520647126502233 1105 42-43 0.5958360147908834 1105 44-45 0.35636101173614776 1105 46-47 0.5759354042781126 1105 48-49 0.7352918048312631 1105 50-51 0.5997305887352766 1105 52-53 0.619603881787782 1105 54-55 0.702130688576446 1105 56-57 0.6252464813065401 1105 58-59 0.6604450232344519 1105 60-61 0.5744467360426952 1105 62-63 0.6297173416170381 1105 64-65 0.6179610685625079 1105 66-67 0.6463973325160381 1105 68-69 0.7932015002907704 1105 70-71 0.8228530978843267 1105 72-73 0.7997628416902813 1105 74-75 0.8140605435116797 1105 76-77 0.7567493590024625 1105 78-79 0.8124488492823936 1105 80-81 0.8005545531543596 1105 82-83 0.7665349718957088 1105 84-85 0.8737675901284234 1105 86-87 1.0074197637465439 1105 88-89 0.8477576428611648 1105 90-91 0.9534641828610226 1105 92-93 1.1232649219429085 1105 94-95 1.181077948944985 1105 96-97 1.262952322954547 1105 98-99 1.4005513332167467 1105 100-101 1.4928481932485944 1106 1 0.316642870182239 1106 2 0.36687946199293364 1106 3 0.36491119795906357 1106 4 0.35369362578079233 1106 5 0.3279397729935951 1106 6 0.19753706421400352 1106 7 0.27238344765812883 1106 8 0.12508827596430905 1106 9 0.32330602120430996 1106 10-11 0.14705647343426875 1106 12-13 0.2152019411050219 1106 14-15 0.17474931870420818 1106 16-17 0.336672723667796 1106 18-19 0.520980300101364 1106 20-21 0.5229735699759885 1106 22-23 0.3860635696507657 1106 24-25 0.3434894085437463 1106 26-27 0.47030379125015287 1106 28-29 0.4459435604752784 1106 30-31 0.4620896867606561 1106 32-33 0.6244001163499107 1106 34-35 0.5713421898423476 1106 36-37 0.486122002727555 1106 38-39 0.585261506228612 1106 40-41 0.6572463513929776 1106 42-43 0.7991153655669905 1106 44-45 0.9271019368038083 1106 46-47 0.9620447227954116 1106 48-49 0.859454573836814 1106 50-51 0.878166223286577 1106 52-53 0.8883065522253943 1106 54-55 0.8992078069465705 1106 56-57 0.8592485822833567 1106 58-59 0.8279060856688218 1106 60-61 0.8153826314442867 1106 62-63 0.9972016541790722 1106 64-65 1.058559354785956 1106 66-67 1.1067456276969878 1106 68-69 0.9786575906233281 1106 70-71 1.260307793602955 1106 72-73 1.1942470294379177 1106 74-75 1.338183584526007 1106 76-77 1.3746044727115354 1106 78-79 1.4842225052537366 1106 80-81 1.4936578880613247 1106 82-83 1.3155148799267877 1106 84-85 1.1976157835285406 1106 86-87 1.3964721625651784 1106 88-89 1.3711966655132066 1106 90-91 1.6077919746186282 1106 92-93 1.806301109063714 1106 94-95 2.0453393331406495 1106 96-97 2.280795270212394 1106 98-99 2.4769448348355922 1106 100-101 2.770455111352625 1107 1 0.2437341650767202 1107 2 0.2826038288197914 1107 3 0.249414030093579 1107 4 0.2905656440125455 1107 5 0.18114080044942682 1107 6 0.21802859573043776 1107 7 0.2294132662130579 1107 8 0.2194857799167309 1107 9 0.26152561545698205 1107 10-11 0.35985613350833034 1107 12-13 0.2837688688236497 1107 14-15 0.4943115506286304 1107 16-17 0.4854171527562343 1107 18-19 0.508254671639726 1107 20-21 0.4926504920059074 1107 22-23 0.5601292531845985 1107 24-25 0.5007034118173692 1107 26-27 0.44771389759043956 1107 28-29 0.5397246432824971 1107 30-31 0.3839027938024273 1107 32-33 0.3198581874970401 1107 34-35 0.5639638948466583 1107 36-37 0.4685023044729064 1107 38-39 0.4665962914017001 1107 40-41 0.5399524882079305 1107 42-43 0.5914130677513825 1107 44-45 0.46492589599873213 1107 46-47 0.5136059740299999 1107 48-49 0.6594640840852009 1107 50-51 0.6488795878902991 1107 52-53 0.6238383234349882 1107 54-55 0.615186856480129 1107 56-57 0.7092944321505641 1107 58-59 0.6742019311735277 1107 60-61 0.8651009448671729 1107 62-63 0.8860200887049388 1107 64-65 0.760116418021255 1107 66-67 0.7638202491192985 1107 68-69 0.7607540245419244 1107 70-71 0.920039177073626 1107 72-73 0.9168204328504288 1107 74-75 0.8608626912955586 1107 76-77 0.8999719696490587 1107 78-79 1.0100042214958442 1107 80-81 0.9727519191153426 1107 82-83 1.0191897184270644 1107 84-85 1.0615171491653044 1107 86-87 1.1447821303524783 1107 88-89 1.2796066479585129 1107 90-91 1.4217470862462598 1107 92-93 1.5121521803190312 1107 94-95 1.6901577310215181 1107 96-97 1.828687441445723 1107 98-99 2.010128403569386 1107 100-101 2.625621662849312 1108 1 0.17707002251525594 1108 2 0.23134682442784893 1108 3 0.24104662133998644 1108 4 0.21164444657703996 1108 5 0.24214085093152704 1108 6 0.18041942818033618 1108 7 0.19906721138949024 1108 8 0.14583743825384943 1108 9 0.25102155164759665 1108 10-11 0.2527394188836638 1108 12-13 0.35305113950868616 1108 14-15 0.3500829171791864 1108 16-17 0.3558630359406507 1108 18-19 0.44400168057505596 1108 20-21 0.4604511129357931 1108 22-23 0.406837188971366 1108 24-25 0.42779210887426444 1108 26-27 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-0.6704531994645357 2316 82-83 -0.685072254261609 2316 84-85 -0.6130631845656538 2316 86-87 -0.7161850832636993 2316 88-89 -0.7098646782063618 2316 90-91 -0.3500822332506708 2316 92-93 -0.48032726388906255 2316 94-95 -0.10489261224699575 2316 96-97 0.1321066058114191 2316 98-99 0.22170481800529274 2316 100-101 0.45994403077643753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8449.0 3 1581.0 4 1327.0 5 1662.0 6 1851.0 7 2180.0 8 2748.0 9 3495.0 10 4480.0 11 6002.0 12 8198.0 13 10800.0 14 12328.0 15 15890.0 16 20202.0 17 27490.0 18 23783.0 19 15325.0 20 14502.0 21 15163.0 22 16216.0 23 18132.0 24 19806.0 25 22666.0 26 26086.0 27 30333.0 28 36835.0 29 43934.0 30 53647.0 31 66759.0 32 84307.0 33 110513.0 34 151136.0 35 213554.0 36 312357.0 37 436640.0 38 353692.0 39 44547.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71813471131274 19.110685506536875 15.120791923108104 22.05038785904227 2 14.866938235910554 20.088331658417484 41.755204330777076 23.28952577489489 3 18.909659357547405 25.16671204267255 30.53346958076153 25.390159019018515 4 12.164170316831143 16.762370151079423 38.18168304707358 32.89177648501585 5 13.047110686069347 37.67158001814188 34.66111127527752 14.62019802051125 6 30.908295043490636 36.48330509149728 17.96807374565519 14.640326119356892 7 27.558312619549934 33.68830954940472 21.085990023370695 17.667387807674647 8 26.018080712414804 34.877656650869845 20.02986298475878 19.07439965195658 9 26.229425282005785 16.975356440456295 21.081169147971465 35.714049129566455 10-11 24.324086006007725 27.115270734870013 27.60118847529881 20.95945478382345 12-13 25.568901901781604 24.587250572857467 28.420332249497527 21.4235152758634 14-15 22.54350718303508 25.587292422650478 27.3033658832968 24.56583451101764 16-17 20.991084859657818 29.184295317212765 27.871372056166415 21.953247766963003 18-19 20.907226369091646 27.182104322081024 31.09248718548348 20.81818212334385 20-21 21.907730051136273 26.044749217896733 31.23360525607407 20.813915474892923 22-23 21.64405709832449 26.12874621890406 31.158960305177153 21.0682363775943 24-25 21.25750459136001 26.313661488782046 31.266937911924646 21.161896007933297 26-27 21.054590168153066 26.50828122255303 31.177205743318464 21.259922865975437 28-29 21.07854019930462 26.633628267883168 31.245635959336987 21.042195573475226 30-31 21.199248268367658 26.371341820375704 31.213467436002556 21.21594247525408 32-33 20.88067539677815 26.495300519250215 31.175221546310215 21.448802537661418 34-35 21.37458765704613 26.48111709275532 31.000962030993108 21.14333321920544 36-37 21.35275980273698 26.24739324615811 31.047422781909802 21.352424169195107 38-39 21.17037299250784 26.61687406622092 30.977489505089533 21.235263436181704 40-41 21.37886207239893 26.633327851447607 30.80090350426748 21.18690657188598 42-43 21.205356543470224 26.532563620366034 30.67223976090504 21.5898400752587 44-45 21.307904431508174 26.70446408752748 30.538244044422484 21.449387436541862 46-47 21.47402814042331 26.90900848428325 29.978136473959893 21.638826901333537 48-49 21.551404286819757 27.140959488206125 29.65141337228896 21.656222852685158 50-51 21.619208261448417 27.430612825395574 29.411514581718745 21.538664331437264 52-53 21.658715944581147 27.536841379625233 29.195167882813056 21.609274792980564 54-55 21.4618415696819 27.4070679365892 29.28281659468286 21.848273899046035 56-57 21.635986677533808 27.718416920167442 29.112747645670005 21.532848756628738 58-59 21.835495359926473 27.199674963291297 29.314449523888786 21.650380152893437 60-61 21.568364662247138 27.24240598361259 29.52324547753203 21.665983876608237 62-63 21.45497173482649 27.248437270647543 29.690716528413745 21.605874466112223 64-65 21.469478564976953 27.199005891222143 29.657742613263 21.6737729305379 66-67 21.4491304143537 27.30183448673974 29.33191926378886 21.917115835117702 68-69 21.507401434842606 27.351108173474408 29.427430093209058 21.71406029847393 70-71 21.429452027056204 27.3533834687404 29.385507509683144 21.831656994520248 72-73 21.419174080503055 27.378658980530936 29.225416873072707 21.97675006589331 74-75 21.450949733870456 27.736542938281396 29.00812037450562 21.80438695334253 76-77 21.491598669535733 27.45123093007186 29.066043444169555 21.991126956222857 78-79 21.558713875872193 27.442834700645836 28.949877143279135 22.048574280202835 80-81 21.466103549526576 27.6555214286002 28.899956902473832 21.978418119399393 82-83 21.56697509737709 27.49906186373719 28.980880816528742 21.95308222235698 84-85 21.550799614339603 27.481480536747267 28.882329947281594 22.08538990163154 86-87 21.50784293938551 27.44401152873939 28.970913897160976 22.077231634714117 88-89 21.557850417664557 27.343887928821932 28.80425382136609 22.29400783214743 90-91 21.454463923698842 27.316790138705777 29.057258088335985 22.171487849259393 92-93 21.465961205924366 27.263614264591634 29.061277168562334 22.209147360921662 94-95 21.36039553271433 27.36252749987022 28.87025431450364 22.40682265291181 96-97 21.40738974031512 27.212163166568743 28.896201672941054 22.484245420175082 98-99 21.349000679742726 27.29066362848368 28.848343982934388 22.511991708839208 100-101 21.539147207299564 26.748669378213847 29.405729062605506 22.306454351881083 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2956.0 1 1730.0 2 536.5 3 581.5 4 495.0 5 456.0 6 854.5 7 1830.0 8 3221.0 9 4092.0 10 3873.5 11 3499.0 12 3585.5 13 3811.5 14 3994.5 15 4195.0 16 4337.5 17 4512.0 18 4721.5 19 4982.5 20 5466.5 21 6166.0 22 7192.0 23 8736.0 24 10573.5 25 12869.0 26 16163.0 27 19928.0 28 23072.5 29 25879.0 30 29423.5 31 33604.5 32 38037.0 33 42535.0 34 47598.5 35 52116.5 36 57175.0 37 62486.5 38 65767.5 39 67529.0 40 69524.0 41 74331.5 42 78323.5 43 80013.0 44 83837.5 45 87760.0 46 88795.0 47 90440.0 48 92791.0 49 90264.0 50 84095.0 51 77221.5 52 70530.0 53 66864.5 54 63806.5 55 58715.5 56 52119.0 57 44896.5 58 39773.0 59 35153.0 60 29002.5 61 23443.0 62 18581.5 63 13730.5 64 9847.0 65 7265.0 66 5139.5 67 3783.5 68 2835.5 69 1935.0 70 1370.5 71 956.5 72 648.5 73 493.5 74 358.0 75 241.5 76 170.5 77 114.5 78 86.5 79 62.0 80 37.5 81 30.0 82 30.5 83 26.5 84 15.0 85 10.0 86 10.5 87 7.5 88 6.0 89 4.5 90 3.5 91 3.0 92 3.0 93 3.0 94 3.0 95 2.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26712873684290467 2 0.2534149371421067 3 0.24251536995319886 4 0.2304990438310013 5 0.22848887775107982 6 0.19418204332041913 7 0.1875708304353439 8 0.1973089683336304 9 0.19864907905357806 10-11 0.20092726727748914 12-13 0.19773333672828047 14-15 0.19491910421639036 16-17 0.1935119879604453 18-19 0.19380234528310064 20-21 0.18321547059551394 22-23 0.17798903878771796 24-25 0.17819005539571012 26-27 0.1875484952566781 28-29 0.19891710119756761 30-31 0.1926632511711451 32-33 0.19824704583759375 34-35 0.1914348163445264 36-37 0.18051291397695277 38-39 0.1825007448782085 40-41 0.17816772021704433 42-43 0.18739214900601753 44-45 0.18212104684089 46-47 0.1821433820195558 48-49 0.19136781080852902 50-51 0.19818004030159642 52-53 0.2078065023065539 54-55 0.18790585811533084 56-57 0.16749150481479447 58-59 0.16959101160937917 60-61 0.17499612484650148 62-63 0.18176368398223727 64-65 0.17222656269194295 66-67 0.1758225264571359 68-69 0.1795971716516552 70-71 0.17597887270779644 72-73 0.17635857074511496 74-75 0.17845807753969967 76-77 0.18819621543798615 78-79 0.19257391045648192 80-81 0.18556066435542237 82-83 0.18482360345945115 84-85 0.17923980879300247 86-87 0.18008854558230267 88-89 0.1837068445261614 90-91 0.1834611575608377 92-93 0.18104895826493186 94-95 0.1815850025529109 96-97 0.17305296430257736 98-99 0.17653725217444133 100-101 0.1723159034066061 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2238621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.26044003509524 #Duplication Level Percentage of deduplicated Percentage of total 1 71.43936985502661 35.905741647435555 2 14.751149848884381 14.827985648571158 3 5.617548398537677 8.470213632868449 4 2.7698442922307582 5.568543718248594 5 1.567969642659978 3.940342210088078 6 0.9195518078183254 2.7730247097609824 7 0.5982801145712595 2.104887527581909 8 0.4040827865597584 1.6247502930480742 9 0.29961140102139516 1.3552740769380034 >10 1.485096311517038 13.317836500918053 >50 0.09661040730066342 3.3412485895603723 >100 0.04812940025117977 4.3349762941089445 >500 0.002133471190386112 0.7211610544221716 >1k 3.5557853173099116E-4 0.36413249448859114 >5k 8.889463293274779E-5 0.2927803971022991 >10k+ 1.7778926586549558E-4 1.057101204858904 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13480 0.6021564168298251 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10180 0.45474423763558014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6553 0.2927248515939054 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2940 0.13133085055487284 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2475 0.11055913439568378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.4670357331589405E-5 0.0 12-13 0.0 0.0 0.0 4.4670357331589405E-5 0.0 14-15 0.0 0.0 0.0 4.4670357331589405E-5 0.0 16-17 0.0 0.0 0.0 6.70055359973841E-5 0.0 18-19 0.0 0.0 0.0 8.934071466317881E-5 0.0 20-21 0.0 0.0 0.0 1.340110719947682E-4 0.0 22-23 0.0 0.0 0.0 3.1269250132112583E-4 0.0 24-25 0.0 0.0 0.0 7.593960746370198E-4 0.0 26-27 0.0 0.0 0.0 0.001183764469287119 0.0 28-29 0.0 0.0 0.0 0.0022781882239110594 0.0 30-31 0.0 0.0 0.0 0.004489370911824735 0.0 32-33 0.0 0.0 0.0 0.007191927530385894 0.0 34-35 0.0 0.0 0.0 0.010184841471602384 0.0 36-37 0.0 0.0 0.0 0.01411583291678225 0.0 38-39 0.0 0.0 0.0 0.020838721695186455 0.0 40-41 0.0 0.0 0.0 0.03390480121467636 0.0 42-43 0.0 0.0 0.0 0.04455868143826043 0.0 44-45 0.0 0.0 0.0 0.05666434827512116 0.0 46-47 0.0 0.0 0.0 0.0796472471222239 0.0 48-49 0.0 0.0 0.0 0.09628695522824096 0.0 50-51 0.0 0.0 0.0 0.11721501763809059 0.0 52-53 0.0 0.0 0.0 0.14441926525302853 0.0 54-55 0.0 0.0 0.0 0.17917280325700508 0.0 56-57 0.0 0.0 0.0 0.2555814494726888 0.0 58-59 0.0 0.0 0.0 0.3258479215552789 0.0 60-61 0.0 0.0 0.0 0.38079246107313386 0.0 62-63 0.0 0.0 0.0 0.448222365465168 0.0 64-65 0.0 0.0 0.0 0.5160766382518523 0.0 66-67 0.0 0.0 0.0 0.6199575542264635 0.0 68-69 0.0 0.0 0.0 0.7427116961736712 0.0 70-71 0.0 0.0 0.0 0.8143629493335407 0.0 72-73 0.0 0.0 0.0 0.9002863816608528 0.0 74-75 0.0 0.0 0.0 1.0489716660390482 0.0 76-77 0.0 0.0 0.0 1.2218906192696308 0.0 78-79 0.0 0.0 0.0 1.3952562760735292 0.0 80-81 0.0 0.0 0.0 1.5517812081634186 0.0 82-83 0.0 0.0 0.0 1.7709786515895276 0.0 84-85 0.0 0.0 0.0 2.0773502973482336 0.0 86-87 0.0 2.2335178665794702E-5 0.0 2.330072843951701 0.0 88-89 0.0 4.4670357331589405E-5 0.0 2.661303543565436 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7260 0.0 44.912216 1 GTATCAA 12035 0.0 39.730946 1 ATCAACG 15125 0.0 31.44703 3 TCAACGC 15260 0.0 31.20136 4 TATCAAC 15275 0.0 31.1424 2 CAACGCA 15470 0.0 30.777813 5 AACGCAG 15905 0.0 30.044792 6 ACGCAGA 17975 0.0 26.426275 7 CGCAGAG 18040 0.0 26.067749 8 GTACATG 10545 0.0 25.016321 1 CTATCGG 115 1.2766142E-4 24.798786 1 TACATGG 10365 0.0 24.43752 2 GCAGAGT 19005 0.0 23.994844 9 GAGTACT 10755 0.0 23.759794 12-13 ACATGGG 10760 0.0 22.256617 3 GTACTTT 11555 0.0 21.928362 14-15 AGTACTT 11175 0.0 21.676716 12-13 AGAGTAC 16685 0.0 21.30841 10-11 CAGAGTA 18490 0.0 20.294373 10-11 CATGGGG 4370 0.0 20.116417 4 >>END_MODULE