FastQCFastQC Report
Thu 26 May 2016
SRR937777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238621
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132990.5940710821528075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114140.5098674585827615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74000.3305606442537616No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.12114600908327046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92400.039.968751
GTATCAA140250.033.4830971
ATCGGCG350.00839627627.14142850-51
ATCAACG174950.026.5808333
TCAACGC177100.026.258144
TATCAAC178700.026.1293622
CAACGCA179250.025.9696885
AACGCAG183450.025.45116
GTACATG115800.023.970431
TACATGG115750.023.8426742
GAGTACT116600.022.83373312-13
ACGCAGA207400.022.4677757
ACATGGG117700.022.3983673
CGCAGAG209550.022.1692528
AGTACTT121200.021.88872112-13
GTACTTT125050.021.11843914-15
GCAGAGT219400.020.8492059
AGAGTAC188750.020.33406610-11
TAGTACG1200.004764489419.791954
TACTTTT138900.018.9100914-15
Produced by FastQC (version 0.11.5)