FastQCFastQC Report
Thu 26 May 2016
SRR937776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783113
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110690.6207682855769656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.476694410281345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.3091783863389477No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23240.13033385994045246No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21380.11990266460959009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59750.042.810211
GTATCAA97600.038.9711461
TATCAAC123100.030.6571032
ATCAACG123100.030.6571033
TCAACGC124150.030.4386734
CAACGCA126100.030.002295
AACGCAG129300.029.3560186
ACGCAGA144700.025.9683657
CGCAGAG144900.025.7030328
GTACATG85750.024.6179491
TACATGG84750.024.3398952
GCAGAGT152000.023.8441039
GAGTACT85450.023.51013412-13
ACATGGG85750.022.8920443
AGTACTT88950.022.37148912-13
AGAGTAC133300.021.57422410-11
GTACTTT93850.021.25405514-15
CATGGGG32950.020.6293684
GTATAGG3750.020.2857821
CAGAGTA147950.020.22445510-11
Produced by FastQC (version 0.11.5)