##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937776_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1783113 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.850737446252705 33.0 31.0 34.0 30.0 34.0 2 31.949318971932794 33.0 31.0 34.0 30.0 34.0 3 31.981539027532186 34.0 31.0 34.0 30.0 34.0 4 35.58704972707843 37.0 35.0 37.0 33.0 37.0 5 35.59750167263656 37.0 35.0 37.0 33.0 37.0 6 35.6590462859056 37.0 35.0 37.0 33.0 37.0 7 35.58246000113285 37.0 35.0 37.0 33.0 37.0 8 35.59009215904993 37.0 35.0 37.0 33.0 37.0 9 37.16515498456912 39.0 37.0 39.0 34.0 39.0 10-11 37.09894661751667 39.0 37.0 39.0 33.5 39.0 12-13 37.00321432236768 39.0 37.0 39.0 33.0 39.0 14-15 38.14004608793722 40.0 38.0 41.0 33.0 41.0 16-17 38.07105494716263 40.0 38.0 41.0 33.0 41.0 18-19 38.03124143001593 40.0 38.0 41.0 33.0 41.0 20-21 38.05243498308856 40.0 38.0 41.0 33.5 41.0 22-23 38.047028427250545 40.0 38.0 41.0 33.0 41.0 24-25 37.975887394685586 40.0 38.0 41.0 33.0 41.0 26-27 37.87255294532652 40.0 38.0 41.0 33.0 41.0 28-29 37.722038928547995 40.0 37.5 41.0 33.0 41.0 30-31 37.46429979479708 40.0 37.0 41.0 32.5 41.0 32-33 37.30981323113005 40.0 37.0 41.0 32.0 41.0 34-35 37.28766572841991 40.0 37.0 41.0 32.0 41.0 36-37 37.13084616622727 40.0 36.5 41.0 31.5 41.0 38-39 36.96074533694724 40.0 36.0 41.0 31.0 41.0 40-41 36.72147586832691 39.5 36.0 41.0 30.5 41.0 42-43 36.43626399448605 39.0 36.0 41.0 30.0 41.0 44-45 36.13673446382815 39.0 35.0 41.0 29.5 41.0 46-47 35.70555259257266 39.0 35.0 41.0 28.0 41.0 48-49 35.29638979694501 39.0 35.0 40.0 26.0 41.0 50-51 34.40796965755956 38.0 34.0 39.5 24.5 40.5 52-53 34.50488723933929 38.0 34.5 39.5 24.0 40.5 54-55 34.77278977832589 39.0 35.0 40.0 24.0 41.0 56-57 34.545592175033214 38.0 34.0 40.0 23.5 41.0 58-59 34.28379889552709 38.0 34.0 40.0 23.0 41.0 60-61 33.9508023327742 38.0 34.0 40.0 22.0 41.0 62-63 33.60492380460464 37.5 33.5 40.0 20.5 41.0 64-65 33.18098628634304 37.0 33.0 40.0 18.5 41.0 66-67 32.66319044278181 36.0 32.0 39.0 13.5 41.0 68-69 32.21842866941131 36.0 31.5 39.0 12.0 40.0 70-71 31.760049138781447 35.0 31.0 38.5 8.0 40.0 72-73 31.293628895084048 35.0 31.0 38.0 7.0 40.0 74-75 30.759179031278443 35.0 30.0 37.0 2.0 39.0 76-77 30.22451493539669 34.5 30.0 36.5 2.0 39.0 78-79 29.716421225127068 34.0 29.0 36.0 2.0 38.5 80-81 29.183191699011786 34.0 29.0 35.5 2.0 37.5 82-83 28.650283521010728 34.0 28.5 35.0 2.0 37.0 84-85 28.116842006087108 34.0 27.0 35.0 2.0 36.5 86-87 27.547668599802705 33.0 26.0 35.0 2.0 36.0 88-89 27.020758919933847 33.0 25.5 35.0 2.0 35.5 90-91 26.47008350003617 32.5 25.0 35.0 2.0 35.0 92-93 25.817713459550795 32.0 23.5 34.5 2.0 35.0 94-95 25.06917200424202 32.0 19.0 34.0 2.0 35.0 96-97 24.215177893941664 31.0 9.0 34.0 2.0 35.0 98-99 23.059486695459007 31.0 2.0 34.0 2.0 35.0 100-101 19.894069248555756 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15971055296259706 1101 2 0.055592839604095445 1101 3 0.11288399863439835 1101 4 0.03889835926919716 1101 5 0.030202222707295334 1101 6 0.0042070089098587005 1101 7 -0.04890972636695068 1101 8 0.06610142258983132 1101 9 0.19979445714004385 1101 10-11 0.3449673984845134 1101 12-13 0.26013251422877204 1101 14-15 0.38992268087841353 1101 16-17 0.44082293601506706 1101 18-19 0.3931010848395786 1101 20-21 0.3806469041579206 1101 22-23 0.32977024111578856 1101 24-25 0.28835473643095355 1101 26-27 0.3652058939428855 1101 28-29 0.35172812098979733 1101 30-31 0.3572815903143791 1101 32-33 0.48247891134300147 1101 34-35 0.5477658486555441 1101 36-37 0.7614671416611358 1101 38-39 0.5167306702417278 1101 40-41 0.6087948685194675 1101 42-43 0.6161876190303062 1101 44-45 0.7657044748743758 1101 46-47 0.8647689853216534 1101 48-49 1.041537083871681 1101 50-51 0.915852566838673 1101 52-53 0.9516884779202996 1101 54-55 0.967744454303407 1101 56-57 1.1769090305044756 1101 58-59 1.207319052404884 1101 60-61 1.166672429733346 1101 62-63 1.1907297364293186 1101 64-65 1.1929409930279675 1101 66-67 1.2330148635197489 1101 68-69 1.3845928752335048 1101 70-71 1.3249602297588545 1101 72-73 1.307549817949333 1101 74-75 1.4034027724576816 1101 76-77 1.3302933899800173 1101 78-79 1.43345207108365 1101 80-81 1.4403100697208124 1101 82-83 1.416716549916142 1101 84-85 1.6983980270598487 1101 86-87 2.087342190064561 1101 88-89 1.8987286279868911 1101 90-91 1.9461047426923272 1101 92-93 1.9649498641315226 1101 94-95 2.395260880338249 1101 96-97 2.376465082963559 1101 98-99 2.6063547607845443 1101 100-101 3.569134070118615 1102 1 0.015532777712149226 1102 2 0.0742755507942725 1102 3 -0.04494426542820307 1102 4 -0.044599053053815396 1102 5 -0.05019023571185244 1102 6 -0.031025978947546662 1102 7 0.0387847048125991 1102 8 -0.043603933116401095 1102 9 0.12264749944560549 1102 10-11 0.19998204443253087 1102 12-13 0.19422267872994325 1102 14-15 0.2978040942560156 1102 16-17 0.2882898945883241 1102 18-19 0.2347786452310885 1102 20-21 0.32156845189182803 1102 22-23 0.1178322587736389 1102 24-25 0.24274293127343327 1102 26-27 0.3082170659612089 1102 28-29 0.26310656543696354 1102 30-31 0.35814054140214324 1102 32-33 0.3506253152506602 1102 34-35 0.29288273514605123 1102 36-37 0.3433670051206974 1102 38-39 0.4847763004633663 1102 40-41 0.565105808490145 1102 42-43 0.584380419543642 1102 44-45 0.7610947006991964 1102 46-47 0.83517111723922 1102 48-49 0.8149273058193458 1102 50-51 0.5969847861795898 1102 52-53 0.6409187789102049 1102 54-55 0.7578515089783835 1102 56-57 0.8770746818953796 1102 58-59 0.8419419055118169 1102 60-61 0.8810892056603521 1102 62-63 1.0125766250458028 1102 64-65 1.040206016513821 1102 66-67 0.9946519787364778 1102 68-69 0.9753428266075339 1102 70-71 0.9768852042504115 1102 72-73 0.845119113993924 1102 74-75 0.8620936385944269 1102 76-77 0.8287275045382749 1102 78-79 1.2032704652053674 1102 80-81 1.4082833264071226 1102 82-83 1.433286858544033 1102 84-85 1.5883514818299425 1102 86-87 1.482304604290615 1102 88-89 1.505736130925449 1102 90-91 1.588349595286175 1102 92-93 1.695258931436971 1102 94-95 1.9040956211860696 1102 96-97 1.8518462879441486 1102 98-99 2.001130748537907 1102 100-101 2.60539821930632 1103 1 0.06218717250206751 1103 2 0.010873554352983206 1103 3 0.10173470011603669 1103 4 0.02457842069463112 1103 5 0.014309681935621654 1103 6 0.14458066217633814 1103 7 0.1264111699450794 1103 8 0.08890398487667994 1103 9 0.00699417388850776 1103 10-11 0.06213589380570994 1103 12-13 0.0688161474994331 1103 14-15 0.23598181506203986 1103 16-17 0.23074454669614397 1103 18-19 0.1106850293070849 1103 20-21 0.15415335599838187 1103 22-23 0.014737018749769959 1103 24-25 0.018718066210084316 1103 26-27 -0.028845925622640323 1103 28-29 0.10724681179252826 1103 30-31 0.12355278330796438 1103 32-33 0.19380551338273477 1103 34-35 0.30730286534405593 1103 36-37 0.4504971815171217 1103 38-39 0.4477276193188544 1103 40-41 0.38691687558989685 1103 42-43 0.4258354334625736 1103 44-45 0.6712761057683281 1103 46-47 0.6249615638059254 1103 48-49 0.6664833298392878 1103 50-51 0.7162580791196831 1103 52-53 0.7360712188401095 1103 54-55 0.8039586307170197 1103 56-57 0.8630204129293162 1103 58-59 1.034078454103856 1103 60-61 1.0282368043416383 1103 62-63 1.0397851915987886 1103 64-65 0.8857590812429095 1103 66-67 1.1223444676458811 1103 68-69 1.2397759509544564 1103 70-71 1.124781569668837 1103 72-73 0.9469308021127496 1103 74-75 1.1751005791575047 1103 76-77 1.342181848836642 1103 78-79 1.4139069692460424 1103 80-81 1.5220903015312608 1103 82-83 1.536504952339513 1103 84-85 1.5471755552289927 1103 86-87 1.8250019938504742 1103 88-89 1.8293216587511552 1103 90-91 1.7814225663763885 1103 92-93 1.9941506824137285 1103 94-95 2.1064145198762603 1103 96-97 2.3417191207901205 1103 98-99 2.6419692660303937 1103 100-101 3.039113884377233 1104 1 0.26308836451700657 1104 2 0.20586054910301144 1104 3 0.22086239092619664 1104 4 0.2378804775985799 1104 5 0.27813075203631143 1104 6 0.21834755703327602 1104 7 0.273677673846656 1104 8 0.17206976590976097 1104 9 0.4188496959336021 1104 10-11 0.2704323257395842 1104 12-13 0.35136617382907787 1104 14-15 0.4197677390730874 1104 16-17 0.44750835380355625 1104 18-19 0.4611695347086524 1104 20-21 0.42188298243653577 1104 22-23 0.28594235808306934 1104 24-25 0.2867817999136264 1104 26-27 0.27714945472109065 1104 28-29 0.2991310969824994 1104 30-31 0.4777433860175293 1104 32-33 0.38577856139844613 1104 34-35 0.6172127860359637 1104 36-37 0.5379255728554639 1104 38-39 0.5844732350336699 1104 40-41 0.666587595426499 1104 42-43 0.8027872182935027 1104 44-45 0.8621421118002104 1104 46-47 1.0241668871818703 1104 48-49 1.0191160950905314 1104 50-51 0.9843487408714822 1104 52-53 0.8937967761509711 1104 54-55 1.1175058325936078 1104 56-57 1.2464080012932044 1104 58-59 1.2395554196519072 1104 60-61 1.2377220597392125 1104 62-63 1.1981450540463356 1104 64-65 1.2787081214075968 1104 66-67 1.3405763520507534 1104 68-69 1.1313187485991527 1104 70-71 1.3251920728044588 1104 72-73 1.2462310717921632 1104 74-75 1.4212658256189599 1104 76-77 1.5028754754662934 1104 78-79 1.561656183345967 1104 80-81 1.6533585527639652 1104 82-83 1.7355790264135855 1104 84-85 1.9437638712485885 1104 86-87 2.181003757138967 1104 88-89 2.169457814941815 1104 90-91 2.231088060176951 1104 92-93 2.2939236889402856 1104 94-95 2.481217047522385 1104 96-97 2.5130184090917744 1104 98-99 2.8885613719234478 1104 100-101 3.523884577086317 1105 1 0.2095744202034986 1105 2 0.26897370607970217 1105 3 0.1910713641834043 1105 4 0.13239053344390328 1105 5 0.09111982405097052 1105 6 0.17593248078129875 1105 7 0.10151227071943225 1105 8 0.1055878794779943 1105 9 0.23759914641448887 1105 10-11 0.2510768443041087 1105 12-13 0.27873286403643505 1105 14-15 0.29188684078304306 1105 16-17 0.2826848806158324 1105 18-19 0.29871395712505233 1105 20-21 0.3516516029804748 1105 22-23 0.29620595708585284 1105 24-25 0.24160069259360029 1105 26-27 0.345758511930633 1105 28-29 0.31966006763038024 1105 30-31 0.310635685942934 1105 32-33 0.37797893521325676 1105 34-35 0.38739538237553006 1105 36-37 0.4444190452773995 1105 38-39 0.30792330749928 1105 40-41 0.35587636878637596 1105 42-43 0.274442313151134 1105 44-45 0.46420766553112713 1105 46-47 0.6014363656846768 1105 48-49 0.6308868654950643 1105 50-51 0.5107465756952578 1105 52-53 0.5735895046303909 1105 54-55 0.6593267191416601 1105 56-57 0.6580155467933935 1105 58-59 0.7502444528102501 1105 60-61 0.8384677040025181 1105 62-63 0.7816453702857231 1105 64-65 0.8002099181578401 1105 66-67 0.9431871705577137 1105 68-69 0.8660442443008414 1105 70-71 0.8236576736167542 1105 72-73 0.7891010080621292 1105 74-75 0.7176736014208309 1105 76-77 0.8311518148039205 1105 78-79 1.1063097523130985 1105 80-81 1.1456202600028682 1105 82-83 1.2848010491544635 1105 84-85 1.556112168519288 1105 86-87 1.4736673578434853 1105 88-89 1.3287598618473453 1105 90-91 1.4025791501676252 1105 92-93 1.344615232160141 1105 94-95 1.3866359441730864 1105 96-97 1.4397660926027989 1105 98-99 1.696678159271606 1105 100-101 2.515293997900258 1106 1 -0.08127873658884255 1106 2 -0.2327409763709234 1106 3 -0.03804854904220889 1106 4 -0.13756383519762494 1106 5 -0.2014678854044476 1106 6 -0.11395521745329518 1106 7 0.027176637116795632 1106 8 0.003952911644361734 1106 9 0.02480904131274997 1106 10-11 -0.40859024781887143 1106 12-13 -0.4423600677783526 1106 14-15 -0.5258980513100155 1106 16-17 -0.32540615300924003 1106 18-19 -0.20200896795723366 1106 20-21 -0.18851486264640016 1106 22-23 -0.12165292232766944 1106 24-25 -0.4243716854734245 1106 26-27 -0.3204435424155676 1106 28-29 0.03217315859387071 1106 30-31 0.020500778888781213 1106 32-33 0.06067735681708797 1106 34-35 0.04786604926661653 1106 36-37 -0.18152946515796486 1106 38-39 -0.2422910570279413 1106 40-41 -0.038956440908414436 1106 42-43 -0.08978627844820153 1106 44-45 -0.06996442453470308 1106 46-47 0.027473006356430574 1106 48-49 0.2540418578274952 1106 50-51 0.38538423652709497 1106 52-53 0.47731832532159046 1106 54-55 0.40293669258571185 1106 56-57 0.23829003624581446 1106 58-59 0.08422181479409119 1106 60-61 0.38457360989719547 1106 62-63 0.25181433722168833 1106 64-65 0.3818133741722036 1106 66-67 0.4136395802300612 1106 68-69 0.3346504774696051 1106 70-71 0.3661799282546916 1106 72-73 0.3601679917171232 1106 74-75 0.4068549720447088 1106 76-77 0.7620116573383271 1106 78-79 0.6835598785473884 1106 80-81 0.813707646859541 1106 82-83 0.6984696514135891 1106 84-85 0.6776436476111485 1106 86-87 0.8091139467124258 1106 88-89 0.9229598637174838 1106 90-91 1.1932458776137622 1106 92-93 1.2364499774473963 1106 94-95 1.4306332698762603 1106 96-97 1.4939083305965148 1106 98-99 1.8222584863586775 1106 100-101 2.135004430463095 1107 1 0.07968356499846152 1107 2 0.12036128884071573 1107 3 0.03908438746572784 1107 4 0.0729189690351788 1107 5 0.05675797438391328 1107 6 0.11018912778479972 1107 7 0.06448235801627078 1107 8 0.028237799210494074 1107 9 0.10523852213285778 1107 10-11 0.12851396018377415 1107 12-13 0.22258598876926783 1107 14-15 0.23667325325347832 1107 16-17 0.1504174663690634 1107 18-19 0.2675212486432983 1107 20-21 0.2652043420493655 1107 22-23 0.3038183078310581 1107 24-25 0.198912871404886 1107 26-27 0.3318743725976674 1107 28-29 0.1666804337261496 1107 30-31 0.12740078715597036 1107 32-33 0.16389329597051727 1107 34-35 0.35167515971635055 1107 36-37 0.303160721680662 1107 38-39 0.32528293437417233 1107 40-41 0.3670455432185733 1107 42-43 0.2473241299512665 1107 44-45 0.3565815410737585 1107 46-47 0.4339141852585513 1107 48-49 0.384617047973002 1107 50-51 0.21096707382867663 1107 52-53 0.17720878497767956 1107 54-55 0.30762625938464794 1107 56-57 0.28494802235692873 1107 58-59 0.3607078432332429 1107 60-61 0.6013188149236441 1107 62-63 0.7035959179095173 1107 64-65 0.5553418133256471 1107 66-67 0.5550344628358843 1107 68-69 0.5506826368611364 1107 70-71 0.7426266875139511 1107 72-73 0.6186780363599844 1107 74-75 0.6919957210526455 1107 76-77 0.6047179238727196 1107 78-79 0.877110432449502 1107 80-81 1.030447684888646 1107 82-83 1.15290706874163 1107 84-85 1.3816212021746388 1107 86-87 1.366818973167451 1107 88-89 1.2406371950666895 1107 90-91 1.4630303104841325 1107 92-93 1.725692288955333 1107 94-95 1.7697743082360446 1107 96-97 2.159329750900749 1107 98-99 2.3798240038851333 1107 100-101 3.256826727090079 1108 1 0.08425815518014446 1108 2 0.10924241806973001 1108 3 0.2587269739227729 1108 4 0.09360658317436332 1108 5 0.17406147453188936 1108 6 0.30462815851305436 1108 7 0.20590667065476964 1108 8 0.17517598178987726 1108 9 0.2433929501917902 1108 10-11 0.25509521645466293 1108 12-13 0.33739466803906737 1108 14-15 0.3468153091663382 1108 16-17 0.3311187411187717 1108 18-19 0.4178772907114805 1108 20-21 0.2742989341078115 1108 22-23 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-1.0381971382731123 2316 78-79 -1.1909189028875211 2316 80-81 -1.423163269683851 2316 82-83 -1.6895195929158007 2316 84-85 -1.940299291861237 2316 86-87 -2.193363863938462 2316 88-89 -2.0216045162134826 2316 90-91 -2.0980205332590174 2316 92-93 -2.4210227481443205 2316 94-95 -2.2326880442263075 2316 96-97 -2.755870931607628 2316 98-99 -3.3742409126797845 2316 100-101 -3.048749961178917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6582.0 3 1350.0 4 1147.0 5 1267.0 6 1506.0 7 1846.0 8 2298.0 9 2735.0 10 3612.0 11 4896.0 12 6672.0 13 8881.0 14 10318.0 15 13449.0 16 17536.0 17 23336.0 18 20166.0 19 13145.0 20 12116.0 21 12743.0 22 13879.0 23 15254.0 24 17322.0 25 19368.0 26 22116.0 27 26075.0 28 30933.0 29 36810.0 30 45181.0 31 55989.0 32 70698.0 33 93364.0 34 125828.0 35 177890.0 36 257842.0 37 346563.0 38 241470.0 39 20928.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79825339988548 19.08480673891107 15.258845776987313 21.85809408421614 2 14.770861058214802 20.098929686046112 41.98225944922994 23.147949806509143 3 18.871227016872894 25.15826245563057 30.698221511774033 25.27228901572251 4 12.15105282924668 16.737488727316887 38.317283581351106 32.79417486208533 5 12.980986827346538 37.58965573658966 34.8071442346238 14.622213201440001 6 30.674193354435182 36.545155912235344 18.09799041707148 14.682660316257994 7 27.40884246107375 33.73699836383904 21.200083606026826 17.654075569060375 8 25.917963580949984 34.82819596633764 20.138193926637086 19.1156465260753 9 26.259468808350363 16.914082978817852 21.155120166279044 35.67132804655274 10-11 24.24068056240015 27.130311695915964 27.719437150305097 20.909570591378788 12-13 25.417110720309566 24.628533264476335 28.614212657418364 21.34014335779574 14-15 22.443483689486346 25.624782607962715 27.481809308621674 24.449924393929262 16-17 20.900268969303113 29.1597954102723 28.05020573033285 21.889729890091736 18-19 20.793299686435486 27.025626216038706 31.379459136323874 20.80161496120194 20-21 21.734071472775838 25.971940251044945 31.636871127022292 20.657117149156925 22-23 21.487710805057663 26.103796900078073 31.491646741165706 20.916845553698558 24-25 21.100338807823483 26.217825898852098 31.61557392247313 21.06626137085129 26-27 20.887230677166592 26.364377042133626 31.58976678588185 21.158625494817933 28-29 20.930095345983197 26.537909131367076 31.576529648959557 20.955465873690173 30-31 21.02872481682795 26.290605081672343 31.578277499970497 21.10239260152921 32-33 20.68302468312021 26.43896367699965 31.5894209981415 21.28859064173865 34-35 21.204977222755105 26.376285592430328 31.375310669033457 21.043426515781118 36-37 21.200774134752148 26.16486126059006 31.389090925436015 21.245273679221775 38-39 21.033643311901514 26.465890371861917 31.363194052726325 21.137272263510248 40-41 21.204619465451902 26.592684956849467 31.16633553175454 21.036360045944093 42-43 21.064294326587806 26.46318838820887 30.997148067866547 21.47536921733678 44-45 21.13897558495262 26.666928861782907 30.89535886551919 21.298736687745283 46-47 21.32598060286097 26.912814012328717 30.270959861148803 21.490245523661507 48-49 21.37413373506636 27.086439094122746 29.93884097924111 21.600586191569786 50-51 21.49294774328909 27.394457761190843 29.72224750718374 21.39034698833633 52-53 21.510308991589508 27.523245875532364 29.469734985499247 21.496710147378877 54-55 21.336723008926135 27.34256213624855 29.563953390368585 21.756761464456726 56-57 21.468210998802004 27.676690219276622 29.389387702363546 21.465711079557824 58-59 21.686399473342455 27.151510657850096 29.594828690253916 21.567261178553533 60-61 21.408649863638903 27.187332252257214 29.858852431985884 21.545165452118002 62-63 21.275527816187644 27.203792507505725 30.0073055990212 21.51337407728543 64-65 21.30476892446394 27.15952144465468 29.941113153530512 21.594596477350866 66-67 21.347283834689424 27.281593186279867 29.589526843404602 21.781596135626106 68-69 21.37685535622896 27.31169198151287 29.70623280595085 21.605219856307325 70-71 21.290536606083723 27.2982786908961 29.645145551025937 21.766039151994242 72-73 21.29833712481087 27.281108890598126 29.50762106649422 21.912932918096782 74-75 21.334598637999537 27.631849156805355 29.255366518364195 21.778185686830913 76-77 21.3299363787374 27.418749942059055 29.286204099887097 21.965109579316447 78-79 21.416026505023627 27.297404501067202 29.27474690837462 22.011822085534547 80-81 21.33515104253282 27.533715453037704 29.21746551483907 21.9136679895904 82-83 21.44192983885239 27.40116120659871 29.272743104809145 21.88416584973975 84-85 21.398384707470488 27.35455822698697 29.22042501843986 22.02663204710268 86-87 21.426053445052883 27.31161019127245 29.249839541249482 22.01249682242518 88-89 21.387791150398723 27.208579926536107 29.12956298084972 22.274065942215447 90-91 21.259234269473392 27.149026130254363 29.413565777043814 22.178173823228434 92-93 21.331842740593327 27.085240463413296 29.370004536383053 22.21291225961032 94-95 21.22792026633319 27.161822355403796 29.2226911292307 22.387566249032318 96-97 21.261203509613683 27.066212861204857 29.145698857976605 22.526884771204852 98-99 21.23851569360351 27.18779794025492 29.056833165817288 22.516853200324285 100-101 21.48048824193917 26.387062453691556 29.85198712716614 22.28046217720313 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2280.0 1 1364.0 2 455.0 3 474.0 4 419.0 5 421.5 6 719.0 7 1510.0 8 2684.5 9 3349.5 10 3105.0 11 2813.5 12 2996.0 13 3255.0 14 3423.5 15 3574.0 16 3655.5 17 3831.5 18 4008.0 19 4198.5 20 4571.5 21 5168.5 22 6065.5 23 7289.5 24 8745.0 25 10688.5 26 13412.0 27 16415.5 28 18946.5 29 21314.0 30 24222.0 31 27684.0 32 31152.5 33 34614.0 34 38734.0 35 41985.5 36 46034.0 37 50290.0 38 52825.0 39 54241.0 40 55654.0 41 59111.5 42 62305.0 43 64274.0 44 67228.5 45 69959.0 46 70629.5 47 71522.5 48 73033.5 49 71131.0 50 66083.5 51 60887.5 52 55895.5 53 52595.0 54 49960.0 55 45772.0 56 40390.0 57 35016.0 58 30828.0 59 26986.0 60 22427.0 61 18001.0 62 14122.0 63 10415.5 64 7463.0 65 5510.0 66 3961.5 67 2914.0 68 2152.5 69 1482.5 70 1044.5 71 724.5 72 511.5 73 377.5 74 271.0 75 199.0 76 143.5 77 93.5 78 66.5 79 47.0 80 30.5 81 22.5 82 16.0 83 10.0 84 7.5 85 6.5 86 5.5 87 6.0 88 4.5 89 3.0 90 4.0 91 3.5 92 0.5 93 1.0 94 1.5 95 0.5 96 2.0 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29493363572583453 2 0.2510216682846236 3 0.2568541645986541 4 0.2510777499799508 5 0.2525919557537857 6 0.19572511669198756 7 0.18714461730692333 8 0.1929210319256267 9 0.19370617566020773 10-11 0.19774405772376735 12-13 0.20074442842377346 14-15 0.1959494434732964 16-17 0.18821016951814046 18-19 0.18260199998541876 20-21 0.18128408014522915 22-23 0.1883503737564585 24-25 0.18748110747888663 26-27 0.19168723462842793 28-29 0.19566903499666036 30-31 0.19639809703591418 32-33 0.19379029820319857 34-35 0.19550078991067868 36-37 0.18616318763869705 38-39 0.1797418335237307 40-41 0.1751711753545625 42-43 0.18321889863401813 44-45 0.18327498032934536 46-47 0.18877098647141263 48-49 0.19572511669198756 50-51 0.1906216824172108 52-53 0.19909001841162058 54-55 0.19039735563590193 56-57 0.17135762007231176 58-59 0.15865511608069707 60-61 0.17365696958072765 62-63 0.1666467576648255 64-65 0.17323635686577352 66-67 0.17146978346296615 68-69 0.17183431448259306 70-71 0.17393737805736373 72-73 0.18192901964149216 74-75 0.18532196220878877 76-77 0.18414424660691722 78-79 0.1808073857349478 80-81 0.18041481386765731 82-83 0.17205864126390197 84-85 0.16835724937230562 86-87 0.17163802854894783 88-89 0.17895668978914964 90-91 0.17533942044054415 92-93 0.17163802854894783 94-95 0.1731241934751191 96-97 0.17957358843774904 98-99 0.17856411792185914 100-101 0.164319367308746 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1783113.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.59861389608027 #Duplication Level Percentage of deduplicated Percentage of total 1 73.5173289542108 39.40426929287867 2 14.384907488121476 15.420222047733137 3 5.123120151051857 8.237764167583709 4 2.3771587545584274 5.096496570210568 5 1.284916003280533 3.443485837436395 6 0.8108155916306838 2.607515510204095 7 0.5147103236117244 1.931143193251394 8 0.34438916344771814 1.4767025441302661 9 0.25959760680902083 1.2522664706132849 >10 1.2568541718090298 12.024521097101223 >50 0.08316276736343721 3.0206832421117973 >100 0.04085096045428959 3.822653247588576 >500 0.0015629026077963975 0.5385814820950711 >1k 3.125805215592795E-4 0.32295901191992904 >5k 2.08387014372853E-4 0.7825738602860047 >10k+ 1.041935071864265E-4 0.6181624248559042 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11069 0.6207682855769656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8500 0.476694410281345 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5513 0.3091783863389477 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2324 0.13033385994045246 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2138 0.11990266460959009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.804084766360853E-5 0.0 22-23 0.0 0.0 0.0 1.9628593364525972E-4 0.0 24-25 0.0 0.0 0.0 4.486535626177365E-4 0.0 26-27 0.0 0.0 0.0 6.449394962629962E-4 0.0 28-29 0.0 0.0 0.0 0.001794614250470946 0.0 30-31 0.0 0.0 0.0 0.004542617321504582 0.0 32-33 0.0 0.0 0.0 0.00726257954487461 0.0 34-35 0.0 0.0 0.0 0.010206868549553506 0.0 36-37 0.0 0.0 0.0 0.01488969010937613 0.0 38-39 0.0 0.0 0.0 0.022432678130886827 0.0 40-41 0.0 0.0 0.0 0.03611661179072779 0.0 42-43 0.0 0.0 0.0 0.04772552272346172 0.0 44-45 0.0 0.0 0.0 0.06157770146928433 0.0 46-47 0.0 0.0 0.0 0.08925401811326596 0.0 48-49 0.0 0.0 0.0 0.1077890184189112 0.0 50-51 0.0 0.0 0.0 0.1294645936628806 0.0 52-53 0.0 0.0 0.0 0.15941221896761448 0.0 54-55 0.0 0.0 0.0 0.19735148585647685 0.0 56-57 0.0 0.0 0.0 0.2827639078398284 0.0 58-59 0.0 0.0 0.0 0.35763297110166326 0.0 60-61 0.0 0.0 0.0 0.41649071034757756 0.0 62-63 0.0 0.0 0.0 0.48676107459258056 0.0 64-65 0.0 0.0 0.0 0.5583213178301094 0.0 66-67 0.0 0.0 0.0 0.6731485890125863 0.0 68-69 0.0 0.0 0.0 0.8035946123436932 0.0 70-71 0.0 0.0 0.0 0.874173425912996 0.0 72-73 0.0 0.0 0.0 0.9678298571094485 0.0 74-75 0.0 0.0 0.0 1.1421598070341028 0.0 76-77 0.0 0.0 0.0 1.3344919811587936 0.0 78-79 0.0 0.0 0.0 1.52873093292461 0.0 80-81 0.0 0.0 0.0 1.708024112885723 0.0 82-83 0.0 0.0 0.0 1.955204185040432 0.0 84-85 0.0 0.0 0.0 2.3213896146794957 0.0 86-87 0.0 0.0 0.0 2.60303188861278 0.0 88-89 0.0 0.0 0.0 2.974292711678957 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5975 0.0 42.81021 1 GTATCAA 9760 0.0 38.971146 1 TATCAAC 12310 0.0 30.657103 2 ATCAACG 12310 0.0 30.657103 3 TCAACGC 12415 0.0 30.438673 4 CAACGCA 12610 0.0 30.00229 5 AACGCAG 12930 0.0 29.356018 6 ACGCAGA 14470 0.0 25.968365 7 CGCAGAG 14490 0.0 25.703032 8 GTACATG 8575 0.0 24.617949 1 TACATGG 8475 0.0 24.339895 2 GCAGAGT 15200 0.0 23.844103 9 GAGTACT 8545 0.0 23.510134 12-13 ACATGGG 8575 0.0 22.892044 3 AGTACTT 8895 0.0 22.371489 12-13 AGAGTAC 13330 0.0 21.574224 10-11 GTACTTT 9385 0.0 21.254055 14-15 CATGGGG 3295 0.0 20.629368 4 GTATAGG 375 0.0 20.285782 1 CAGAGTA 14795 0.0 20.224455 10-11 >>END_MODULE