FastQCFastQC Report
Thu 26 May 2016
SRR937776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783113
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107320.6018687542516935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89320.5009217026627029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.3269562837576755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21870.12265066768062372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70200.039.7432631
GTATCAA107500.034.9728131
ATCAACG135550.027.6485273
TATCAAC136300.027.4615382
TCAACGC138050.027.1478274
CAACGCA140550.026.6311475
AACGCAG145250.025.8006736
GTACATG92350.024.034911
GAGTACT91100.023.8830812-13
TACATGG91900.023.6208322
ACGCAGA162400.023.021417
CGCAGAG163100.022.8060998
TAAGGTG6900.022.7174385
GTACTTT95850.022.52225714-15
ACATGGG92200.022.2044933
GTAAGGT6500.021.9231224
AGTACTT93700.021.62333512-13
GCAGAGT171550.021.295069
GTACCGT1404.85495E-420.3560436
AGAGTAC149950.020.33910410-11
Produced by FastQC (version 0.11.5)