##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937776_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1783113 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30894228240162 33.0 31.0 34.0 31.0 34.0 2 32.536766879048045 34.0 31.0 34.0 31.0 34.0 3 32.62939869767087 34.0 31.0 34.0 31.0 34.0 4 36.218241917365866 37.0 35.0 37.0 35.0 37.0 5 36.07370705053466 37.0 35.0 37.0 35.0 37.0 6 36.16988379311911 37.0 36.0 37.0 35.0 37.0 7 36.13428874109493 37.0 36.0 37.0 35.0 37.0 8 36.1316282254686 37.0 36.0 37.0 35.0 37.0 9 37.814842917975476 39.0 38.0 39.0 35.0 39.0 10-11 37.770318258012814 39.0 37.5 39.0 35.0 39.0 12-13 37.69537712977248 39.0 37.0 39.0 35.0 39.0 14-15 38.944042525627935 40.0 38.0 41.0 36.0 41.0 16-17 38.92264175069107 40.0 38.0 41.0 35.5 41.0 18-19 38.92921200170713 40.0 38.0 41.0 35.5 41.0 20-21 38.860867202471184 40.0 38.0 41.0 35.0 41.0 22-23 38.717743911911356 40.0 38.0 41.0 34.5 41.0 24-25 38.66010426708795 40.0 38.0 41.0 34.5 41.0 26-27 38.58824090228718 40.0 38.0 41.0 34.0 41.0 28-29 38.47371843511881 40.0 38.0 41.0 34.0 41.0 30-31 38.52900993935886 40.0 38.0 41.0 34.5 41.0 32-33 38.43659543730543 40.0 38.0 41.0 34.0 41.0 34-35 38.30494337711632 40.0 38.0 41.0 34.0 41.0 36-37 38.15408053219285 40.0 38.0 41.0 34.0 41.0 38-39 38.019584288825214 40.0 38.0 41.0 33.5 41.0 40-41 37.839311642055215 40.0 37.0 41.0 33.0 41.0 42-43 37.62091017226614 40.0 37.0 41.0 33.0 41.0 44-45 37.29826012148417 40.0 37.0 41.0 32.5 41.0 46-47 36.89877141830047 40.0 37.0 41.0 31.5 41.0 48-49 36.52748311520358 40.0 36.0 41.0 30.5 41.0 50-51 36.26860131691036 39.5 36.0 41.0 30.0 41.0 52-53 36.04729789979659 39.0 36.0 41.0 29.5 41.0 54-55 35.837173807829345 39.0 35.0 40.5 29.0 41.0 56-57 35.57566065639138 39.0 35.0 40.0 28.0 41.0 58-59 35.326770372937666 38.5 35.0 40.0 28.0 41.0 60-61 34.99404748885797 38.0 34.5 40.0 27.0 41.0 62-63 34.6457064134466 38.0 34.0 40.0 26.0 41.0 64-65 34.256075470259034 37.0 34.0 40.0 26.0 41.0 66-67 33.84287955951193 37.0 33.5 39.0 25.5 41.0 68-69 33.3984901685984 36.0 33.0 39.0 24.5 40.0 70-71 32.98842585971837 35.5 33.0 39.0 23.5 40.0 72-73 32.54945339975649 35.0 32.0 38.0 22.5 40.0 74-75 32.062061125683 35.0 32.0 37.0 21.5 39.0 76-77 30.822189900471816 34.0 30.5 36.0 20.0 38.5 78-79 31.36591904158626 34.5 31.0 36.0 20.0 39.0 80-81 31.158721012072707 35.0 31.0 36.0 19.5 37.5 82-83 30.81181478683628 34.0 31.0 35.5 18.0 37.0 84-85 30.434662862084455 34.0 31.0 35.0 16.5 37.0 86-87 30.02621847297395 34.0 30.5 35.0 9.5 36.0 88-89 29.61510880129302 34.0 30.0 35.0 7.0 36.0 90-91 29.136964959595943 34.0 29.0 35.0 2.0 35.0 92-93 28.701199250972877 34.0 29.0 35.0 2.0 35.0 94-95 28.269069879474827 34.0 29.0 35.0 2.0 35.0 96-97 27.77136446203914 33.0 28.0 35.0 2.0 35.0 98-99 27.018937666878095 33.0 26.5 35.0 2.0 35.0 100-101 24.663099029618426 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14598529132810967 1101 2 0.1374215955846836 1101 3 0.08766695153966708 1101 4 0.080111203606549 1101 5 0.006160596634501303 1101 6 0.031733518902797186 1101 7 0.04274028245706063 1101 8 0.06878455853716048 1101 9 0.19225599857331588 1101 10-11 0.1275683042958704 1101 12-13 0.12251064464637551 1101 14-15 0.17604076032960592 1101 16-17 0.04570703724606773 1101 18-19 0.20704096520156412 1101 20-21 0.20892988293909553 1101 22-23 0.14365313875897812 1101 24-25 0.07194514325245649 1101 26-27 0.05841068403035621 1101 28-29 0.07825768427450441 1101 30-31 -3.284190699233136E-4 1101 32-33 0.15411221678010634 1101 34-35 0.009373009386123954 1101 36-37 -0.03602640911022803 1101 38-39 0.10953626655133064 1101 40-41 0.07508716121033387 1101 42-43 0.07137033900500711 1101 44-45 0.1077433718057037 1101 46-47 0.06635925272672694 1101 48-49 0.16555190996457725 1101 50-51 0.19784810448438606 1101 52-53 0.08655723596944398 1101 54-55 0.07086244360941407 1101 56-57 0.13157796054495918 1101 58-59 0.12284086479404976 1101 60-61 0.1888536713426987 1101 62-63 0.048433982308417 1101 64-65 0.12995279812049176 1101 66-67 0.24932423187392772 1101 68-69 0.023931219818500438 1101 70-71 0.01583404133064903 1101 72-73 0.062136328106014105 1101 74-75 0.14392992614996913 1101 76-77 0.01162339661827616 1101 78-79 -0.04446587045522321 1101 80-81 -0.04151327699155516 1101 82-83 -0.16607217418724574 1101 84-85 0.09087553793418479 1101 86-87 0.2580581456999482 1101 88-89 0.3460064824393534 1101 90-91 0.33006877849449623 1101 92-93 0.510773018279604 1101 94-95 0.46108733455358575 1101 96-97 0.5523260410542719 1101 98-99 0.6897038071985229 1101 100-101 1.3105013724102612 1102 1 0.08698987476994802 1102 2 0.2295553677256521 1102 3 0.11451527147205098 1102 4 0.01941565516054311 1102 5 -0.10713371099308944 1102 6 -0.10546990307303616 1102 7 -0.07233460799719182 1102 8 -0.02590328858925517 1102 9 0.11496332451980606 1102 10-11 0.06603949090521866 1102 12-13 0.03307165585975014 1102 14-15 0.010774176935584023 1102 16-17 0.03251615269284258 1102 18-19 0.08400417812128325 1102 20-21 0.010203463303120941 1102 22-23 0.11095403237828094 1102 24-25 -0.10153486711246984 1102 26-27 -0.0639007522159929 1102 28-29 -0.07364418884305479 1102 30-31 0.020374450734614413 1102 32-33 0.11421824400935776 1102 34-35 0.04498886616924125 1102 36-37 0.11100543589150647 1102 38-39 0.013383501526874397 1102 40-41 -0.013071720670232878 1102 42-43 0.0861288860287388 1102 44-45 0.03331413729154775 1102 46-47 0.019380266253463674 1102 48-49 -0.10986509406831146 1102 50-51 -0.061604073086662936 1102 52-53 -0.15686097256585185 1102 54-55 -0.0675714900149984 1102 56-57 0.072101453834577 1102 58-59 0.09943739590465839 1102 60-61 0.18344811137508543 1102 62-63 0.18127318317534247 1102 64-65 0.0840763772244415 1102 66-67 0.12243710809515562 1102 68-69 0.03611565989611876 1102 70-71 0.07914860708620353 1102 72-73 0.12416335574430803 1102 74-75 0.08869647949605408 1102 76-77 -0.016776107881483426 1102 78-79 0.10093240046347418 1102 80-81 0.11606571839915247 1102 82-83 0.14518475380173257 1102 84-85 0.15784512877169732 1102 86-87 0.05173993606612726 1102 88-89 0.16671284168219813 1102 90-91 0.2871071694240257 1102 92-93 0.49637262332688437 1102 94-95 0.16774398739389085 1102 96-97 0.4499740303946851 1102 98-99 0.638700658303545 1102 100-101 1.3938535530184168 1103 1 0.17812138500731578 1103 2 0.1747794611053166 1103 3 0.14281112765483783 1103 4 0.11033419635975861 1103 5 -0.04606526119167853 1103 6 0.03312400175983754 1103 7 0.044692384731483514 1103 8 0.11003238463591458 1103 9 0.07733598186038648 1103 10-11 0.12855045999196335 1103 12-13 0.08292902398094526 1103 14-15 0.2248109291138789 1103 16-17 0.17667627840869926 1103 18-19 0.13315969344559875 1103 20-21 0.08140853054867847 1103 22-23 0.20164509159379662 1103 24-25 0.16095571634844674 1103 26-27 0.24807610672356617 1103 28-29 0.166865526055183 1103 30-31 0.14522105227527504 1103 32-33 0.11113137424245423 1103 34-35 0.09882480643260294 1103 36-37 -0.02294162628330554 1103 38-39 -0.03225211823664864 1103 40-41 0.003785065583095104 1103 42-43 0.01410923752553117 1103 44-45 0.059322010733261266 1103 46-47 0.18471477952301996 1103 48-49 0.09496201287387862 1103 50-51 0.044607906371105344 1103 52-53 0.06158568999669001 1103 54-55 -0.014506929682994496 1103 56-57 0.19882849435472139 1103 58-59 0.12477109239913631 1103 60-61 0.08749408433644845 1103 62-63 0.14127550524663235 1103 64-65 0.04079995488827848 1103 66-67 0.16014730954833567 1103 68-69 0.0400603122469505 1103 70-71 0.025721391870696664 1103 72-73 0.04832764633803066 1103 74-75 0.005277249775698323 1103 76-77 -0.08097597881751994 1103 78-79 -0.05734004600063258 1103 80-81 0.08155022127339251 1103 82-83 0.024334443697210162 1103 84-85 0.03211610639070983 1103 86-87 0.07228047916079916 1103 88-89 0.28525255585142517 1103 90-91 0.3450274913279081 1103 92-93 0.5141030551511427 1103 94-95 0.6877435013789679 1103 96-97 0.6459072330440065 1103 98-99 0.7922008770102558 1103 100-101 1.4838208212635529 1104 1 0.13990549785777517 1104 2 0.18596956115798235 1104 3 0.1471056526839547 1104 4 0.06942350971447553 1104 5 -0.002258313356293229 1104 6 0.04117050151250368 1104 7 0.09830114213171015 1104 8 0.09723458036891941 1104 9 0.18118449862359398 1104 10-11 0.12340022142940654 1104 12-13 0.10124807547535397 1104 14-15 0.14053710876185477 1104 16-17 0.10811148354058986 1104 18-19 0.13984483075869747 1104 20-21 0.09909626891177226 1104 22-23 0.11032709328528512 1104 24-25 0.048216954320587035 1104 26-27 0.03434419838266223 1104 28-29 0.08835630269999228 1104 30-31 0.13496146589010038 1104 32-33 0.1254531065736657 1104 34-35 0.16923666594399123 1104 36-37 0.2096907799273069 1104 38-39 0.03396195775230382 1104 40-41 -0.041698402589936734 1104 42-43 0.23465992608570474 1104 44-45 0.10983902925975286 1104 46-47 0.2360117141138005 1104 48-49 0.4310370204536227 1104 50-51 0.314136725366744 1104 52-53 0.1600416157630491 1104 54-55 0.21180137452815018 1104 56-57 0.3930076476571003 1104 58-59 0.43911217303168826 1104 60-61 0.34995578666782734 1104 62-63 0.32134236659311455 1104 64-65 0.30501896170206777 1104 66-67 0.3842694551752217 1104 68-69 0.29026875528714413 1104 70-71 0.4361646873850802 1104 72-73 0.4496682642070837 1104 74-75 0.6691204607160621 1104 76-77 0.3570875155255955 1104 78-79 0.4245363801885915 1104 80-81 0.41303848463369164 1104 82-83 0.4836032773736285 1104 84-85 0.49181379416896576 1104 86-87 0.5834306858086364 1104 88-89 0.6783880976836478 1104 90-91 0.6109299998252062 1104 92-93 0.6565165263560822 1104 94-95 0.6953910670834063 1104 96-97 1.0339184670268047 1104 98-99 1.4128116051446824 1104 100-101 2.0615880272890514 1105 1 0.12968444227055187 1105 2 0.137775670959698 1105 3 0.11064098215435081 1105 4 0.05292588221895045 1105 5 0.011442793623380965 1105 6 0.023320702459230347 1105 7 0.0742775844244079 1105 8 0.06723154560527433 1105 9 0.11778913699038185 1105 10-11 0.09244811397041275 1105 12-13 0.013161933567538142 1105 14-15 0.1477049652260476 1105 16-17 0.19034755128104308 1105 18-19 0.14360035076686728 1105 20-21 0.09726087505901404 1105 22-23 0.17698269866389893 1105 24-25 0.08194750134381934 1105 26-27 -0.007479524393097847 1105 28-29 0.06334112888178112 1105 30-31 0.09109246841150309 1105 32-33 0.08431397825989251 1105 34-35 0.045811126848008143 1105 36-37 0.04632902759238533 1105 38-39 0.10327014509081778 1105 40-41 0.08449529474485473 1105 42-43 0.12492414905857885 1105 44-45 0.1404383808981038 1105 46-47 0.3535024467397747 1105 48-49 0.21376637909745 1105 50-51 0.2563358228790946 1105 52-53 0.29723172243356544 1105 54-55 0.27057723028895 1105 56-57 0.3956017324206016 1105 58-59 0.4062615656535229 1105 60-61 0.4167595849439678 1105 62-63 0.45726680665085695 1105 64-65 0.561192011299184 1105 66-67 0.6333363362606974 1105 68-69 0.4357523462917712 1105 70-71 0.5009381011538352 1105 72-73 0.7147158702890906 1105 74-75 0.8308712357152892 1105 76-77 0.4893464858047629 1105 78-79 0.6213287484670857 1105 80-81 0.608415220759575 1105 82-83 0.5751084917782627 1105 84-85 0.568410377649073 1105 86-87 0.8337476342545216 1105 88-89 0.8020664155737229 1105 90-91 0.7034286029830632 1105 92-93 1.207148863322626 1105 94-95 1.2619913660218103 1105 96-97 1.349286779556408 1105 98-99 1.6111681530017705 1105 100-101 1.998240526998341 1106 1 -0.05533295000952165 1106 2 0.11531765554976658 1106 3 -0.015950972513515183 1106 4 0.040422843245281115 1106 5 -0.13001675529942247 1106 6 -0.14712247340850837 1106 7 -0.09761611695201822 1106 8 0.011238455763859179 1106 9 -0.022779758880353995 1106 10-11 -0.01661539122857647 1106 12-13 -0.04662169150726925 1106 14-15 -0.11912978006120767 1106 16-17 -0.037920864899383844 1106 18-19 0.10399434201462299 1106 20-21 0.12407806442914904 1106 22-23 0.003287820970903965 1106 24-25 -0.022371698424279884 1106 26-27 -0.08333698250201138 1106 28-29 -0.0021060911586161524 1106 30-31 -0.18749083219442042 1106 32-33 -0.18991949907404404 1106 34-35 -0.2912642350236254 1106 36-37 -0.16671003958295927 1106 38-39 -0.23163527269793605 1106 40-41 -0.2749363938024203 1106 42-43 -0.2805824712286764 1106 44-45 -0.3005856597212855 1106 46-47 -0.21924999846519455 1106 48-49 -0.2979383448702322 1106 50-51 -0.3838169673662648 1106 52-53 -0.23042293794944158 1106 54-55 -0.30904738633283557 1106 56-57 -0.46790393409645503 1106 58-59 -0.2909847735520401 1106 60-61 -0.2832346877595029 1106 62-63 -0.1673708541809802 1106 64-65 -0.2630951156083583 1106 66-67 -0.25258351431530457 1106 68-69 -0.5446474423826757 1106 70-71 -0.2865517299727358 1106 72-73 -0.047556323569374115 1106 74-75 -0.20228338258961998 1106 76-77 -0.19640435108182785 1106 78-79 -0.20443929368581593 1106 80-81 -0.11947040162223033 1106 82-83 -0.1044369946445407 1106 84-85 0.019805500330100756 1106 86-87 -0.0016259023627682723 1106 88-89 0.3178375200770098 1106 90-91 0.23129248501477662 1106 92-93 0.4830602047723538 1106 94-95 0.2588845998638192 1106 96-97 0.6724190964699304 1106 98-99 0.9248358201920759 1106 100-101 1.7917405129723072 1107 1 0.11426857115449707 1107 2 0.15758369390955096 1107 3 0.07571156055526984 1107 4 0.10179643782199577 1107 5 -0.11226295238937212 1107 6 0.04340538704122565 1107 7 0.07475491479401342 1107 8 0.08658361118714453 1107 9 0.20630423582863955 1107 10-11 0.20127172021322082 1107 12-13 0.061885252937180724 1107 14-15 0.19486864917160318 1107 16-17 0.005590419822837589 1107 18-19 0.13655675934266043 1107 20-21 0.11231281145295924 1107 22-23 0.12976558221429002 1107 24-25 0.28222796262068783 1107 26-27 0.07419443284189953 1107 28-29 0.08900357319323149 1107 30-31 0.08151855107277584 1107 32-33 0.12042069603177907 1107 34-35 0.2465220166298181 1107 36-37 0.10972431888264822 1107 38-39 0.12710935362482445 1107 40-41 0.14922758602561714 1107 42-43 0.34338412930042495 1107 44-45 0.38159233300358153 1107 46-47 0.35050963281787517 1107 48-49 0.19422848714035013 1107 50-51 0.39177000353320324 1107 52-53 0.26432739273839445 1107 54-55 0.15979561961955113 1107 56-57 0.3391603846866147 1107 58-59 0.20590986103790954 1107 60-61 0.47677378611615495 1107 62-63 0.3496389011100334 1107 64-65 0.5718244859128063 1107 66-67 0.5386646255656515 1107 68-69 0.42630369849033656 1107 70-71 0.5755049416542519 1107 72-73 0.5764361119464922 1107 74-75 0.6030706600691111 1107 76-77 0.4809528331112922 1107 78-79 0.5378980492374623 1107 80-81 0.5512471909703613 1107 82-83 0.46198475634752256 1107 84-85 0.5950369818115817 1107 86-87 0.9248838942642124 1107 88-89 0.9343326424315102 1107 90-91 0.9124577462581627 1107 92-93 1.0641872302353157 1107 94-95 1.4155272916627624 1107 96-97 1.6581426827794559 1107 98-99 1.790938250747633 1107 100-101 2.5458999458426774 1108 1 0.057804413564831236 1108 2 0.1757268614388039 1108 3 0.058843323189080365 1108 4 0.05877724439352505 1108 5 -0.07086877610433362 1108 6 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2316 82-83 -0.2199984314048926 2316 84-85 -0.4774454858631927 2316 86-87 -0.5349580800008376 2316 88-89 -0.4623068647849209 2316 90-91 -0.48137932834814023 2316 92-93 -0.5227402297690666 2316 94-95 -0.249274608716064 2316 96-97 -0.5180583597677391 2316 98-99 -0.9787445237428365 2316 100-101 -1.2147071109182228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 7.0 5 34.0 6 71.0 7 140.0 8 391.0 9 754.0 10 1233.0 11 2226.0 12 3432.0 13 5205.0 14 6640.0 15 9782.0 16 13542.0 17 20834.0 18 18863.0 19 10386.0 20 8249.0 21 8294.0 22 8407.0 23 8855.0 24 10179.0 25 11807.0 26 14066.0 27 17566.0 28 21846.0 29 27639.0 30 34690.0 31 44444.0 32 58023.0 33 77361.0 34 109466.0 35 162015.0 36 261289.0 37 421602.0 38 352995.0 39 30771.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95197515042833 20.2027331835596 15.320853934702544 21.52443773130952 2 15.351354625309781 21.174260969439402 40.40618850291597 23.06819590233485 3 19.197885944412945 25.528051222777243 30.547699444735134 24.726363388074677 4 12.736153008810996 17.645768944536886 37.26039796692638 32.35768007972574 5 13.083025501178419 38.52441362321344 34.040870537742485 14.351690337865655 6 30.993773249367816 37.29729972245169 17.272152690266964 14.436774337913524 7 27.737165283411652 34.28823635966986 20.445759747138855 17.528838609779637 8 26.39238231116031 35.5514204652201 19.335398261355284 18.72079896226431 9 26.469494642235237 17.196554564965876 20.603910127961605 35.73004066483728 10-11 24.608956358907147 27.757242530338793 26.866553045151935 20.76724806560212 12-13 25.77074234277181 25.17846652131545 27.92552035501216 21.12527078090057 14-15 22.6404327712265 26.28372402646383 26.813275434591077 24.26256776771859 16-17 21.082763683513047 29.913611756517955 27.3586418808006 21.644982679168397 18-19 21.05374140618121 27.790527016515497 30.587657652655775 20.56807392464751 20-21 21.984906164054756 26.59345010296605 30.870130423663795 20.5515133093154 22-23 21.69634847946547 26.81682221448915 30.7311705722569 20.755658733788476 24-25 21.463093872766887 26.91372633692351 30.82311473525115 20.80006505505845 26-27 21.249096717730918 27.148718187429004 30.77401721346849 20.82816788137159 28-29 21.184013424030788 27.398404213853517 30.759655672712977 20.65792668940272 30-31 21.31012255006427 27.10032146117902 30.72710682695608 20.86244916180063 32-33 21.142406566493545 27.139362452071182 30.686950294232613 21.03128068720266 34-35 21.49190769177276 27.05709060502615 30.70601807064387 20.74498363255722 36-37 21.574600151532742 26.910240685811836 30.57756294749688 20.937596215158546 38-39 21.388380882198714 27.2956901777958 30.513826100757495 20.80210283924799 40-41 21.502142601169975 27.409956632025 30.332373775526282 20.755526991278735 42-43 21.475637314481766 27.36118067587221 30.1012221025054 21.061959907140626 44-45 21.489832669045654 27.506389107140155 29.939914071626422 21.063864152187776 46-47 21.71788888309378 27.712573460010663 29.391350968783247 21.17818668811231 48-49 21.759922113741528 27.99127144493927 28.94306193718514 21.30574450413406 50-51 21.812162212938834 28.31211482390628 28.731045088000588 21.144677875154294 52-53 21.945334352151082 28.487237905628497 28.44080224887661 21.126625493343802 54-55 21.695820735982522 28.45321636934956 28.47203177813184 21.37893111653608 56-57 21.789280881245325 28.6894324700678 28.403359742203666 21.117926906483213 58-59 21.9567408234924 28.134924707519936 28.58612998727506 21.3222044817126 60-61 21.72007606921154 28.236208249280892 28.803474597515695 21.240241083991872 62-63 21.591845272845863 28.207494421273356 28.988796559724484 21.2118637461563 64-65 21.64419753543382 28.229085873974334 28.840067903716704 21.286648686875147 66-67 21.542605544348564 28.37296346333631 28.662345011224748 21.422085981090376 68-69 21.66993903358901 28.32035883311938 28.669495427378973 21.34020670591264 70-71 21.66489168100956 28.374421587414815 28.572081522595592 21.388605208980024 72-73 21.640822797676982 28.307119324843118 28.437845866621352 21.614212010858548 74-75 21.644069469556594 28.816221302980406 28.003372755048478 21.53633647241452 76-77 21.68586623506194 28.394610997732617 28.324817327897893 21.59470543930755 78-79 21.742480706494767 28.464180340786026 28.113922112619893 21.679416840099307 80-81 21.7178669321201 28.75345778799711 27.99276545927416 21.535909820608627 82-83 21.66679098137018 28.679906836878732 28.102852938322897 21.55044924342819 84-85 21.613803519187396 28.674611023826063 28.02669828930961 21.684887167676933 86-87 21.569833207429927 28.5937290569919 28.045193994996392 21.79124374058178 88-89 21.49782431062978 28.61719924648634 27.855721987333386 22.02925445555049 90-91 21.487834371112534 28.561493325197112 27.975730436899248 21.974941866791102 92-93 21.37728728597924 28.50732139021448 28.089220627650285 22.026170696155997 94-95 21.27184311930876 28.606319397592863 27.957145733332663 22.16469174976572 96-97 21.342618218811708 28.53335711197215 27.900923833767127 22.223100835449014 98-99 21.223906888500117 28.63991246751812 27.67850207741028 22.457678566571488 100-101 20.92385078142068 28.61620377654176 28.14791506637175 22.312030375665813 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 529.0 1 366.5 2 263.0 3 369.5 4 355.5 5 366.0 6 644.0 7 1395.5 8 2317.5 9 2911.5 10 3029.5 11 2999.0 12 3150.0 13 3409.0 14 3685.0 15 3792.0 16 3888.0 17 3977.0 18 4099.0 19 4277.5 20 4576.5 21 5166.5 22 5962.0 23 7213.0 24 8652.0 25 10552.0 26 13206.5 27 16072.0 28 18744.0 29 21498.5 30 24354.5 31 27277.0 32 30854.0 33 34436.0 34 38410.0 35 41769.0 36 45904.5 37 50313.0 38 53038.5 39 54488.5 40 55522.5 41 59193.5 42 62752.0 43 64689.5 44 67604.0 45 69551.5 46 69668.0 47 71616.0 48 74108.5 49 70896.0 50 65735.0 51 60980.5 52 55622.5 53 53094.0 54 50778.0 55 46584.5 56 40880.0 57 35239.0 58 31265.5 59 27564.0 60 22877.0 61 18295.5 62 14400.5 63 10434.0 64 7196.0 65 5224.5 66 3769.0 67 2861.5 68 2143.0 69 1456.5 70 996.0 71 637.0 72 413.5 73 316.0 74 242.5 75 158.0 76 111.5 77 76.0 78 52.0 79 27.0 80 12.5 81 8.5 82 5.5 83 3.5 84 2.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048734993239351626 2 0.0 3 0.0 4 0.0 5 0.01205756449535167 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004234167997204889 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.608169532721706E-5 22-23 3.6453101962691096E-4 24-25 4.486535626177365E-4 26-27 0.0018226550981345544 28-29 0.002355431203743117 30-31 2.804084766360853E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011328502456097846 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.804084766360853E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.41225429908256E-5 74-75 5.608169532721706E-5 76-77 0.0 78-79 0.0 80-81 2.804084766360853E-5 82-83 2.2432678130886825E-4 84-85 1.4020423831804266E-4 86-87 0.0 88-89 0.0 90-91 0.007795355650483172 92-93 0.0028040847663608533 94-95 0.0 96-97 0.0 98-99 1.9628593364525975E-4 100-101 0.01337548433554127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1783113.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.12312948276507 #Duplication Level Percentage of deduplicated Percentage of total 1 71.70138625803531 36.65599253763291 2 14.935675836142345 15.271169793674211 3 5.55402674464731 8.51817685252034 4 2.6250568470384015 5.368044843630529 5 1.470724971048972 3.7594031564236254 6 0.9007347003108254 2.7629026028165953 7 0.5765893247291374 2.0633935494555438 8 0.40506641130888027 1.6566610075570285 9 0.28246192662070524 1.2996303883723441 >10 1.4050831551641159 12.782964084222659 >50 0.09285270258742341 3.209699307224942 >100 0.04803825775271496 4.392842096267553 >500 0.001644903295678353 0.5473867914209459 >1k 3.2898065913567067E-4 0.28249508828043285 >5k 2.193204394237804E-4 0.8275833485206887 >10k+ 1.096602197118902E-4 0.6016545519796795 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10732 0.6018687542516935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8932 0.5009217026627029 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5830 0.3269562837576755 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2187 0.12265066768062372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.804084766360853E-5 0.0 22-23 0.0 0.0 0.0 2.2432678130886825E-4 0.0 24-25 0.0 0.0 0.0 5.047352579449536E-4 0.0 26-27 0.0 0.0 0.0 6.729803439266047E-4 0.0 28-29 0.0 0.0 0.0 0.0018787367934617718 0.0 30-31 0.0 0.0 0.0 0.004794984950477059 0.0 32-33 0.0 0.0 0.0 0.007655151412165129 0.0 34-35 0.0 0.0 0.0 0.010655522112171242 0.0 36-37 0.0 0.0 0.0 0.015618752148629952 0.0 38-39 0.0 0.0 0.0 0.023329985256122298 0.0 40-41 0.0 0.0 0.0 0.03732236824026296 0.0 42-43 0.0 0.0 0.0 0.049211687649632976 0.0 44-45 0.0 0.0 0.0 0.06331623402442807 0.0 46-47 0.0 0.0 0.0 0.09188985779364515 0.0 48-49 0.0 0.0 0.0 0.11070526657592648 0.0 50-51 0.0 0.0 0.0 0.13325010809746773 0.0 52-53 0.0 0.0 0.0 0.16378659120313743 0.0 54-55 0.0 0.0 0.0 0.20287553284620774 0.0 56-57 0.0 0.0 0.0 0.2890730985641403 0.0 58-59 0.0 0.0 0.0 0.36500771403719223 0.0 60-61 0.0 0.0 0.0 0.42479080125600566 0.0 62-63 0.0 0.0 0.0 0.4961267177122257 0.0 64-65 0.0 0.0 0.0 0.5695376568955528 0.0 66-67 0.0 0.0 0.0 0.6857669704612103 0.0 68-69 0.0 0.0 0.0 0.81786740380447 0.0 70-71 0.0 0.0 0.0 0.8898763006046168 0.0 72-73 0.0 0.0 0.0 0.9850188967272405 0.0 74-75 0.0 0.0 0.0 1.1624613807425552 0.0 76-77 0.0 0.0 0.0 1.3574013536999618 0.0 78-79 0.0 0.0 0.0 1.554079859212512 0.0 80-81 0.0 0.0 0.0 1.735952797158677 0.0 82-83 0.0 0.0 0.0 1.9851798511928296 0.0 84-85 0.0 0.0 0.0 2.3555153262861075 0.0 86-87 0.0 0.0 0.0 2.6422329936465045 0.0 88-89 0.0 0.0 0.0 3.020980723038865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7020 0.0 39.743263 1 GTATCAA 10750 0.0 34.972813 1 ATCAACG 13555 0.0 27.648527 3 TATCAAC 13630 0.0 27.461538 2 TCAACGC 13805 0.0 27.147827 4 CAACGCA 14055 0.0 26.631147 5 AACGCAG 14525 0.0 25.800673 6 GTACATG 9235 0.0 24.03491 1 GAGTACT 9110 0.0 23.88308 12-13 TACATGG 9190 0.0 23.620832 2 ACGCAGA 16240 0.0 23.02141 7 CGCAGAG 16310 0.0 22.806099 8 TAAGGTG 690 0.0 22.717438 5 GTACTTT 9585 0.0 22.522257 14-15 ACATGGG 9220 0.0 22.204493 3 GTAAGGT 650 0.0 21.923122 4 AGTACTT 9370 0.0 21.623335 12-13 GCAGAGT 17155 0.0 21.29506 9 GTACCGT 140 4.85495E-4 20.356043 6 AGAGTAC 14995 0.0 20.339104 10-11 >>END_MODULE