FastQCFastQC Report
Thu 26 May 2016
SRR937775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464320
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109590.4447068562524348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87430.35478346967926244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56850.23069244253992988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30970.1256736138163875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53500.055.816321
GTATCAA97650.042.9001661
ATCAACG125200.033.032213
TCAACGC126400.032.646724
CAACGCA127950.032.2512365
TATCAAC129050.032.230272
AACGCAG132200.031.3352996
ACGCAGA151950.026.9519827
CGCAGAG152700.026.6634988
GTACATG111000.025.4459551
GCAGAGT164000.024.4492249
TACATGG112300.024.3389572
TGGTATC23100.023.870412
ACATGGG112850.023.3783653
GTACCTG18650.023.2015951
GAGTACT95450.021.8488512-13
CAGAGTA157850.021.65362510-11
AGTACTT98750.021.3351912-13
TACCTGG19350.020.8810272
GTACTAG4850.020.5888881
Produced by FastQC (version 0.11.5)