FastQCFastQC Report
Thu 26 May 2016
SRR937775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464320
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129990.5274883132060771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112530.4566371250486949No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74010.3003262563303467No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.12068237891182965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.060.1570321
GTATCAA107450.044.4509121
ATCAACG139300.034.112083
TCAACGC141800.033.4771584
TATCAAC143950.033.0101622
CAACGCA144350.032.9186865
AACGCAG149350.031.8342236
TGGTATC23450.029.382212
ACGCAGA172550.027.4449987
CGCAGAG173550.027.0952788
GTGGTAT28100.025.7074091
GTACATG110650.025.6415311
GCAGAGT185150.024.9102769
TACATGG112050.024.6390292
ACATGGG112650.023.4954243
GAGTACT119350.023.222112-13
CAGAGTA180050.021.84340110-11
GTACTTT126550.021.69357314-15
GGACCGA8400.021.48676
AGTACTT123700.021.25352512-13
Produced by FastQC (version 0.11.5)