FastQCFastQC Report
Thu 26 May 2016
SRR937774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415437
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106550.44112100626097883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86020.3561260343366438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.23101409807003867No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30900.12792716183448377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53000.055.96941
GTATCAA98400.043.4799771
ATCAACG126750.033.5568923
TCAACGC129100.032.8772134
TATCAAC129600.032.7456132
CAACGCA130450.032.5727355
AACGCAG135350.031.5904456
CGCAGAG154800.027.40648
ACGCAGA156650.027.1433937
GTACATG109700.026.8674831
TACATGG110500.025.2884222
GCAGAGT169400.024.3146579
ACATGGG111150.024.0716363
GAGTACT99800.022.94166412-13
TGGTATC24550.022.2614292
CATGGGG50850.021.7788414
GGACCGA6350.021.6968026
GTACTTT105850.021.38358114-15
CAGAGTA160900.021.34472810-11
AGTACTT104300.020.6766412-13
Produced by FastQC (version 0.11.5)